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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Nat Neurosci. Author manuscript; available in PMC 2012 February 1.
Published in final edited form as:
PMCID: PMC3145042
NIHMSID: NIHMS293757

Axin2 as regulatory and therapeutic target in newborn brain injury and remyelination

Abstract

Permanent damage to white matter tracts, comprising axons and myelinating oligodendrocytes, is an important component of newborn brain injuries that cause cerebral palsy and cognitive disabilities as well as multiple sclerosis (MS) in adults. However, regulatory factors relevant in human developmental myelin disorders and in myelin regeneration are unclear. Here, we report expression of AXIN2 in immature oligodendrocyte progenitor cells (OLP) within white matter lesions of human newborns with neonatal hypoxic-ischemic and gliotic brain damage, as well as active MS lesions in adults. Axin2 is a target of Wnt transcriptional activation that feeds back negatively on the pathway, promoting β-catenin degradation. We show Axin2 function is essential for normal kinetics of remyelination. Small molecule inhibitor XAV939, which targets enzymatic activity of Tankyrase, acts to stabilize Axin2 levels in OLP from brain and spinal cord and accelerates their differentiation and myelination after hypoxic and demyelinating injury. Together, these findings indicate that Axin2 is an essential regulator of remyelination and that it might serve as a pharmacological checkpoint in this process.

Introduction

Oligodendrocytes are the myelinating cells of the central nervous system (CNS) that enable formation of myelin and saltatory nerve conduction. In humans, premyelinating oligodendrocytes are most abundant at gestational age 23-32 weeks and are thought to be selectively vulnerable during injury in the neonatal brain1. Hypoxic ischemic encephalopathy (HIE) causes neuronal apoptosis as well as diffuse primary damage to sub-cortical white matter in the term infant brain, while periventricular leukomalacia (PVL) comprises focal injury to white matter tracts as well as diffuse gliotic lesions typically in premature infants2. Such diffuse and focal injuries, collectively known as white matter injury (WMI) in the newborn brain, can result in cerebral palsy (CP) and cognitive disability. Indeed, WMI is the most reliable prognostic indicator of development of severe CP in premature infants3. In multiple sclerosis (MS), the most common cause of neurological disability in young adults, myelin sheaths are lost through the injury or death of mature oligodendrocytes as a result of autoimmune damage4.

In these conditions, myelin sheaths can be regenerated by OLP that are recruited to lesions and differentiate in a process called remyelination. Conversely, inhibition of remyelination may contribute to ongoing neurological dysfunction, axonal loss and disease progression5,6. Although oligodendrocytes are thought to be cellular targets of excitotoxic damage in newborn brain injuries2,7, several lines of evidence indicate failure of remyelination as contributing to fixed demyelinated lesions8,9. Indeed, both PVL8,9 and MS5,6 lesions show presence of non-myelinating OLP.

The molecular mechanisms that might dysregulate myelination in neonatal brain injury are unknown. We have previously shown that active Wnt signaling can act to inhibit oligodendrocyte OLP differentiation during remyelination in the adult rodent CNS10, and several studies show that Wnt activation inhibits developmental myelination10-12. In the absence of Wnt ligands, β-catenin levels are regulated through a phosphorylation/degradation complex13,14 containing glycogen synthase kinase 3β (GSK-3β) and the scaffolding proteins, Axis inhibition protein 1 (Axin1), adenomatous polyposis coli (APC) and disheveled (Dsh). The presence of Wnt ligand results in stabilization of β-catenin and its translocation to the nucleus, where it forms a nucleoprotein complex with Tcf/LEF transcription factors to activate or repress expression of target genes15,16. While Axin1 is expressed ubiquitously, Axin2/conductin is a transcriptional target of active Wnt signaling that also serves to auto-regulate and repress the pathway by promoting β-catenin degradation in many different tissues and systems14,17-19. Dual roles for signaling targets that also serve as feedback repressors are seen in other pathways such Sonic hedgehog (Shh), where the repressors Patched and Gli3 are activated by Shh20.

We observed expression of AXIN2 mRNA transcripts in situ in OLP in WMI associated with neonatal HIE and PVL. Although Axin2 function has been reported as dispensable during brain development21, we show that Axin2 function is essential for normal myelination and remyelination. Moreover, Axin2 levels can be manipulated pharmacologically in OLP to promote accelerated differentiation, suggesting Axin2 serves as a therapeutic target in situations where OLP differentiation is delayed or stalled.

Results

AXIN2 mRNA marks OLP in human neonatal white matter injury

We have previously shown Wnt pathway activation using the transgenic Bat-Gal reporter in developing OLP in vivo, and that forced activation of the canonical Wnt pathway during development of transgenic Olig2-cre X floxed-Dominant-active β-catenin mice inhibits differentiation of OLPs to mature oligodendrocytes (OL)10. Expression profiling of P4 spinal cord from these mice indicated significant downregulation of genes associated with OL maturation (Suppl. Tab. 1, Suppl. Fig. 1), including MBP, PLP, CNPase, MOG, MAG, Mobp, FA2H, MAL and transcription factor MRF22. This analysis also identified known Wnt-activated targets, such as Naked1, Notum and Axin2 as significantly upregulated. In contrast, changes in expression of the Wnt pathway antagonist adenomatous polyposis coli (APC, also known as CC1) were not significant, indicating that its expression in Olig2-cre X floxed-Dominant-active β-catenin mice is uncoupled from OL differentiation.

Using expression of AXIN2 mRNA transcripts to mark Wnt-activated white matter cells in situ, we investigated two types of human neonatal brain injury characterized by white matter damage and injury to oligodendrocyte lineage cells resulting in hypomyelination (Suppl. Fig.2). As shown, (Fig. 1a, b, c), we observed AXIN2 mRNA expression solely in Olig2-positive cells within affected white matter in neonatal HIE and PVL (Note, in PVL staining localized adjacent to the cystic core of lesion) but not white matter in age-matched controls (Fig. 1a, c). Within the HIE cases, AXIN2 mRNA was expressed in a subset of the Tcf4 positive cells (Fig. 1d), consistent with activation of canonical Wnt signaling. AXIN2 mRNA segregated from the mature OL marker PLP in situ (Fig. 1e), and segregated completely from GFAP (Fig. 1f). Similarly, the independent Wnt activated target Naked1 (Nkd1) was expressed in PDGFRα-positive OLP, but not astroglia (Fig. 1g, h, Suppl. Fig. 3). The expression of these Wnt targets provides strong evidence that the Wnt pathway is activated specifically in immature, pre-myelinating OLPs in human neonatal white matter injury (Suppl. Fig. 4). We thus, went on to address possible functions of Axin2 in OLP in animal models.

Figure 1
AXIN2 mRNA expression identifies Wnt pathway activation in immature oligodendrocytes within neonatal human white matter injury

Axin2 function is required for OLP differentiation

Whilst Axin2 mRNA serves as a marker for pathway activation, Axin2 protein functions as a negative feedback mechanism to control activation of the Wnt pathway via β-catenin degradation17,18. Using wild type and Axin2-lacZ heterozygous mice18 we characterized Axin2 mRNA and reporter gene expression during developmental myelination (Fig. 2a-d). Axin2 mRNA is expressed in immature Nkx2-2+ OLP but not CC1+ differentiated OL (Fig. 2b). In contrast, reporter β-galactosidase proteins are first detectable at the later CC1+ stage (Fig. 2c, d; Suppl. Fig. 6).

Figure 2
Axin2 functions as a negative regulator of Wnt signaling in OLPs and promotes differentiation

Axin2-lacZ homozygous null animals showed a significant delay in OLP differentiation during developmental myelination (Fig. 2e, f; Suppl. Fig. 7). Moreover, as indicated by co-expression of β-galactosidase with PDGFRα (Fig. 2g), the reduction in mature OL number reflects delay in OLP maturation, rather than decreased OLP numbers. Indeed, there is no difference in apoptosis or proliferation of the OLP pool (Suppl. Fig. 8). We also observed a significant (p < 0.005) impairment of Axin2−/− OL differentiation in vitro (Fig. 2h, i), and strong activation of the independent Wnt target Notum (Fig. 2j). Thus, Axin1 is unable to compensate for the loss of early Axin2 functions in OL differentiation in vitro or in vivo.

Axin2 is expressed in MS and functions in myelin repair

Previous studies indicate that lesions of both neonatal PVL and adult MS show OLP, evidently ‘stalled’ in their differentiation5,6,8,9, which has been proposed to result in defective repair and fixed demyelinated lesions23. Thus, we focused on regulatory functions of Axin2 in the context of primary damage to oligodendrocytes. We confirmed that AXIN2 mRNA transcripts are expressed in OLP in active MS lesions (Fig. 3a, b), in which OL are targeted for autoimmune attack. Axin2 mRNA expressing cells were not seen in areas of normal appearing white matter (NAWM), nor in chronic silent plaques (Fig. 3a). A similar pattern of expression was seen for the independent Wnt activated target Naked1 (Nkd1). Naked1 protein expressing cells were seen at active MS lesion edges (Fig. 3c, Suppl. Fig. 5), with the simple bipolar morphology characteristic of OLP (Fig. 3c), and at a similar cellular density to Axin2 mRNA expressing cells (Fig. 3c). Remyelination can be investigated after adult murine lysolecithin injury, which kills resident OL while leaving axons largely intact. Such lesions in spinal cord ventral or dorsal white matter have been extensively characterized24 and show OLP recruitment (5 days post lesion (dpl)), differentiation (10 dpl) and myelination (14 dpl) with stereotyped timing in young adult animals (Fig. 4a), allowing precise assessment of remyelination kinetics.

Figure 3
AXIN2 is expressed in OLP in active MS lesions
Figure 4
Axin2 function is essential for timely myelin repair

In adult animals, we observed robust expression of the β-gal reporter in Axin2-lacZ heterozygote mice 10 days after demyelination (Fig. 4b). Although mature OL numbers normalize by 8 weeks of age in Axin2−/− animals (Fig. 4f, Suppl. Fig. 9), Axin2−/− null mice showed significantly delayed remyelination compared to WT littermates (Fig. 4c, Suppl. Fig. 10), due to a delay in Nkx2.2+ OLP differentiation (with normal OLP recruitment) in lesions at 10 and 14 dpl (Fig. 4c-f). The inflammatory cell and astrocyte response in lesions was not affected in Axin2−/− mice (Suppl. Fig. 11), demonstrating that the delay in remyelination in Axin2 null animals is attributable to a cell-autonomous requirement in OLP.

Axin2 protein stabilization promotes OLP differentiation

We next investigated whether enhanced Axin2 activity might promote accelerated oligodendrocyte maturation. Axin2 and Axin1 were recently identified as substrates for the poly-ADP-ribosylating enzymes Tankyrase 1 and 225, which promote Axin degradation through the ubiquitin-proteosome pathway. Small molecule XAV939 inhibited tankyrase activity in cell lines at 5μM concentration, resulting in stabilized Axin protein levels25.

As shown (Fig 5a, b), Tankyrase is expressed in the rodent OL lineage during development commencing at a mature stage, and continues to be expressed in oligodendrocytes in adult white matter (S. Fancy and D. Rowitch, unpublished observations). Tankyrase is also expressed within early differentiating OLs following demyelination in the adult spinal cord white matter of the mouse (Fig. 5c and inset).

Figure 5
Axin protein stabilization through small molecule Tankyrase inhibition promotes OLP differentiation in vitro

Given these findings, we tested effects of XAV939 to stabilize Axin protein levels in OLP in vitro. As shown (Fig. 5d), treatment with 0.01 or 0.1μM XAV939 for 24 hours produced increases in the levels of both Axin2 and Axin1 proteins in OLP versus vehicle controls, and increased protein levels of Tankyrase 1 and 2, presumably due to reduction in their autoparsylation and self-induced degradation25. XAV939 treatment of OLP resulted in increased levels of phospho-β-catenin and β-catenin degradation, as well as reduced levels of Axin2 mRNA transcripts (Fig. 5e) and activity of a transduced Topflash reporter (data not shown), indicating inhibition of the Wnt pathway. Moreover, in addition to early effects on Wnt pathway per se, XAV939 treatment promoted precocious OL differentiation (Fig. 5f-h), as evidenced by increased expression of the mature oligodendrocyte marker myelin basic protein (MBP).

We next confirmed Tankyrase protein expression within the human OL lineage during neonatal white matter injury. As shown (Fig. 5i), Tankyrase was expressed in later stage OL cells that expressed NOGO-A+ and cytoplasmic Olig1+. Conversely, Tankyrase expression was not observed in inflammatory macrophages, microglia or reactive astrocytes within gliotic areas of white matter damage. These data suggest the potential for pharmacological manipulation of Axin2 levels in OLPs through Tankyrase inhibition in human neonatal white matter injuries.

To address this question, we investigated XAV939 effects on OLP in mouse cerebellar slice cultures (Fig. 6) In this system, slices of P0-P1 neonatal cerebellum are cultured in the presence of factors that can affect remyelination26 of cerebellar white matter. We examined the ratio of NFH-positive axons that showed co-expression of myelin basic protein (MBP) and measured the density of Nodes of Ranvier, indicated by Caspr-positive paranodes, As shown (Fig. 6a, a’), 0.01μM XAV939 treatment significantly increased myelination compared with controls. Moreover, we observed that acute hypoxic exposure (2% oxygen for 24 hrs) reduced myelination to levels significantly below controls (Fig. 6b, a’), We suggest the impact of hypoxia in this system is primarily due to inhibition of OLP maturation because Nkx2.2-Olig2 double positive OLP numbers were significantly increased without detectable reduction in Olig2-positive cells (Fig. 6b’). Treatment with XAV939 reversed the impact of hypoxia, and indeed, increased the extent of myelinated axons to well above control levels. To demonstrate that XAV939 treatment can act on cerebellar OLP to promote remyelination, we also tested its effects after addition of 0.5% lysolecithin to culture medium to induce toxic injury to oligodendrocytes (Fig. 6c). In this paradigm, XAV939 also promoted a significant enhancement of myelin regeneration (Fig. 6c’).

Figure 6
XAV939 treatment increases myelination, myelination following hypoxia, and remyelination in ex vivo mouse cerebellar slice cultures

As illustrated above (Fig. 4a), the kinetics of remyelination in vivo in focal toxic injury models is tightly regulated and to our knowledge no drugs have been reported that accelerate OLP differentiation. To test whether pharmacologic stabilization of Axin might promote OLP differentiation during remyelination in vivo, we co-injected young adult (8-10 week old) mouse spinal cord lysolecithin lesions with 0.1μM XAV939. As shown (Fig. 7a, c), we observed a striking increase in PLP+ differentiated OLs as early as 6 dpl in XAV939-treated dorsal and ventral lesions compared to vehicle treated controls. Moreover, such XAV939 effects are Axin2-dependent, as Axin2 null mice show significantly less OLP differentiation at 6dpl following lysolecithin treatment versus controls (Fig. 7b, c). This increase in mature OL number at 6 dpl was due to a precocious differentiation of recruited OLP rather than an increased survival of existing OL (Fig. 7g). Whilst total Olig2+ cells were similar at 6 dpl in XAV939 treated lesions and controls, XAV939 produced a shift in the proportion of Olig2+ cells that expressed the mature PLP+ OL versus immature Nkx2.2+ OLP (Fig. 7d, e). XAV939 treatment did not affect the inflammatory cell or astrocyte infiltration into lesions (Fig. 7f), nor early OLP recruitment into or proliferation within the lesion (Fig. 7h, i). Such precocious OLP differentiation in XAV939 treated lesions was associated with significantly thicker myelin sheaths (smaller g ratios) compared to controls at 10 dpl when myelin sheath formation is on-going (Fig. 7j). Myelin thickness in XAV939-treated remyelinated lesions was similar to controls when lesions in both groups are fully remyelinated at 28 dpl (Suppl. Fig. 12). These findings indicate that XAV939 treatment significantly accelerates the processes of OLP differentiation and compact myelin formation in vivo.

Figure 7
XAV939 treatment dramatically accelerates OLP differentiation and myelin regeneration during remyelination in vivo

Discussion

Despite advances in neonatal intensive care in developed countries, newborn brain injuries such as HIE in full term infants and PVL in very premature infants remain leading causes of cerebral palsy and cognitive disability. Indeed, the incidence of these conditions is rising27, owing to the increasing survival of extremely low birth weight premature infants born at gestational ages <28 weeks. While WMI and demyelination is primary in MS, it is also a significant component of HIE and PVL. Progression in MS lesions to a stage of chronic demyelination is thought to reflect both autoimmune-mediated damage to oligodendrocytes as well as inefficient repair. In both MS5,6 and PVL8,9 there is evidence for ‘stalled’ OL precursors that fail to engage in remyelination.

The presence of AXIN2-and Nkd1-positive OLP in neonatal HIE and PVL as well as MS, suggests a generalized Wnt pathway activation response in white matter to diverse types (both toxic, auto immune and hypoxic) of injury. However, further work is needed to confirm this proposal in other human neuropathological conditions (e.g., spinal cord injury). Because Axin2 expression serves dual roles as a readout of the Wnt pathway and a feedback inhibitor, the presence of Axin2+ OLP likely signifies cells attempting to myelinate axons. Our studies show Axin2 to be a potent regulator of the timing of remyelination, an important determinant of effective repair and functional recovery in human MS and animal models28. Several lines of evidence indicate that the Wnt pathway acts cell autonomously in OLP. First, we have previously activated β-catenin within OLP in vivo and observed delayed differentiation10. Second, dispersed cultures of Axin2−/− OLP showed delayed differentiation, and third, wild type OLP cultures treated with XAV939 show accelerated differentiation (Suppl. Fig. 13).

It is unclear whether the effect of dysregulated Wnt signaling is sufficient to account for stalled OLP maturation, or as more likely, that Wnt signals are amongst several factors that may be involved. The abnormal environment of a demyelinated axon tract, containing inflammatory cells, myelin debris29 and other factors (e.g., Lingo-130, Notch31 signaling), appear to also inhibit remyelination. Delayed remyelination kinetics might lead to chronic demyelination23, in keeping with recent studies showing that oligodendrocytes initiate new myelin segments only during a temporally restricted developmental window32.

Despite probably complex roles of multiple factors in remyelination, treatment with XAV939 alone was sufficient to stabilize Axin proteins and promote dramatically accelerated oligodendrocyte differentiation after demyelinating injury. Our data in Axin2−/− animals demonstrate that XAV939 acts specifically through its effects on Axin2, and our in vitro studies indicate that this acts to inhibit the canonical Wnt pathway through β-catenin phosphorylation/degradation. XAV939 was non-toxic to OLP at early stages of recruitment and proliferation and acted to accelerate differentiation and formation of compact myelin. These findings indicate that Wnt pathway activity is dispensable for myelin regeneration.

Our therapeutic approach employed targeted injection of XAV939 into spinal cord lesions. Although this approach might seem to present technical challenges, we note that non-invasive imaging currently allows for precise neurosurgical placement of devices, neural progenitor cells and drugs directly into white matter tracts of the brain. In preliminary studies, we observe upregulation of Tcf4, Axin2, Nkd1 and Notum in the white matter of neonatal mice exposed to chronic hypoxia, and that Axin2−/− animals sustain permanent deficits in white matter after such injury (J. Silbereis, E. Harrington, S. Fancy and D. Rowitch, unpublished observations). XAV939 treatment promoted robust myelination of axons after cerebellar cultures were exposed to hypoxia, suggesting efficacy of XAV939 to act on OLP from the brain to promote myelination after a developmental hypoxic insult. Further study of systemic XAV939 (and similar agents that stabilize Axin2) administration is needed to determine possible toxicity and efficacy in promoting remyelination in pre-clinical models of human neonatal hypoxic white matter injury and adult demyelinating diseases33.

Methods

Transgenic Mice and procedures

Animal husbandry and procedures were performed according to UCSF guidelines under IACUC approved protocols.

Axin2-LacZ

The Axin2-LacZ mouse has been described previously (Lustig, B, et al. Mol Cell Biol 22:1184–1193 (2002)). Insertion of β-galactosidase gene into Axin2 locus (Axin2-LacZ) provides a useful tool for visualizing cells that are actively responding to Wnt in vivo. LacZ insert mimics the expression pattern of Axin2 but does not lead to detectable phenotype in heterozygous state. In homozygous state, this effectively acts as an Axin2 null animal.

Olig2-tva-cre

A multi-functional mouse line was constructed previously (Schüller, U, et al. Cancer Cell 14:123-134 (2008)). Olig2-cre allowed for cre mediated activity in oligodendrocyte lineage cells.

DA-Cat

The DA-Cat mouse was produced previously (Harada, N, et al. EMBO J. 18(21):5931-5942 (1999)) and has exon 3 of mouse β-catenin gene located between loxP sequences. Cre recombinase mediated deletion of exon 3 produces dominant stable mutant β-catenin protein.

Induction of demyelination with lysolecithin in mouse spinal cord

Demyelinated lesions were produced in ventrolateral spinal cord white matter of 8-10 week old Axin2 null and wild-type littermate mice. The method has been described previously (Fancy, SPJ, et al. Genes and Development 23, 1571-1585 (2009)). Animals were euthanased at three survival time points: 5 days post lesion (dpl), representing peak OLP recruitment, 10 dpl representing onset of OLP differentiation and 14 dpl representing new myelin sheath formation (n=4 for each survival time).

Immunohistochemistry

The method has been described previously (Fancy, SPJ, et al. Genes and Development 23, 1571-1585 (2009)). The primary antibodies were: Tcf4 (mouse monoclonal 6H5-3, Upstate), Olig2 (rabbit polyclonal from CD Stiles, Harvard), Nkx2.2 (mouse monoclonal, Developmental Studies Hybridoma Bank), PDGFRα (rat 558774, BD Biosciences), APC (CC1)(mouse monoclonal OP80, Calbiochem), NOGO-A (rabbit, Millipore), β-gal (rabbit 55976, MP), Tankyrase (mouse monoclonal 19A449, Abcam), Iba1 (rabbit, Wako), CD3 (rabbit, Dako), GFAP (mouse, Sigma), Naked1 (rabbit, Cell Signaling Technology) and Notum (rabbit, Abcam). Secondary fluorescent antibodies from Alexa were used.

Cell counts and statistical measures

For all cell counts, at least four animals per genotype were used for each time point indicated. Cells were counted on three or more non-adjacent sections per mouse and presented as an average +/− standard deviation. Statistical comparisons of cell counts were established using Student t test.

Human MS tissue

Human post-mortem tissue blocks were provided by the UK Multiple Sclerosis Tissue Bank at Imperial College London. All tissues were collected following fully informed consent by the donors via a prospective donor scheme following ethical approval by the London Multicentre Research Ethics committee (MREC 02/2/39). MS lesions were characterized according to Lock et al. Nat. Med. 8: 500-508 (2002), using Luxol Fast Blue to assess demyelination, SMI-31 immunohistochemistry to assess preservation of axons, and LN3 immunohistochemistry to assess inflammatory cell activity. Lesions with florid parenchymal and perivascular inflammatory cell infiltration, myelin fragmentation, and demyelination with indistinct margins were classified as active plaques (AP). Chronic active plaques (CAP) were classified as those with extensive demyelination well demarcated borders and abundant inflammatory cells at the lesion edge. Chronic plaques (CP) were classified as those with extensive demyelination, well demarcated borders but few or no inflammatory cells in any part of the demyelinated area.

Human Developmental Tissue

All human tissue was collected in accordance with guidelines established by the University of California San Francisco Committee on Human Research (H11170-19113-07). Immediately after procurement, all brains, except Case 1, were immersed in 4% phosphate buffered formalin for one week at room temperature. In Case 1, the brain was immersed in phosphate buffered saline with 4% paraformaldehyde for three days. On day 3, brains were cut in coronal plane at level of Mamillary Body and immersed in fresh 4% paraformaldehyde/PBS for additional three days. Post fixation, all tissue samples were equilibrated in PBS with 30% sucrose for at least 2 days, before OCT embedding. The diagnosis of hypoxic ischemic encephalopathy (HIE) requires clinical and pathological correlations. With respect to the pathological features, all HIE cases in study showed consistent evidence of diffuse white matter injury, including astrogliosis and macrophage infiltration. These findings were confirmed by increase in number and staining intensity of GFAP- or CD68-positive cells, respectively. The HIE cases also showed evidence of neuronal injury, including presence of ischemic neurons and variable degrees of neuronal loss, in cerebral cortex, hippocampus and basal ganglia. Representative images in Supplementary Figure 2 show GFAP immunoreactivity most intense in the Layer 1 of cerebral cortex and in the subcortical white matter.

Case 1, 2, 3, 4 and 5 demonstrated clinical evidence of HIE and pathological evidence of HIE and low output state in multiple organs on post-mortem examination. More specific findings in Case 1 (8 week-old, born at full term) included hypoplastic left heart status-post procedure and diffuse white matter injury in brain with focal neuron dropout in cerebral and cerebellar cortices. Case 2 (five-day old, born full term) with a clinical diagnosis of severe HIE who underwent therapeutic hypothermia showed diffuse white matter injury on post-mortem evaluation. Case 3 (five month old, born preterm at 26 weeks gestation) clinically showed failure to thrive, enterocolitis, hypoxemia and ventriculomegaly. Decreased myelination relative to the patient’s age, diffuse white matter gliosis, and focal loss of cortical neurons were noted in this patient. Case 4 (6 day old male born at 35 and 5/7 weeks) with in utero polyhydramnios showed neuropathological findings of hypoxic ischemic encephalopathy and intraventricular hemorrhage. Case 5 (6 day old female born at 37 and 4/7 weeks) with intrauterine growth retardation showed hypoxic ischemic encephalopathy characterized by widespread neuronal death in the putamen, thalamus, hippocampus, diffuse hypoxic white matter changes, and diffuse cerebral edema. Case 6 (12 week old, born at 35 3/7 weeks) with congenital diaphragmatic hernia, hypoplastic heart, and coarctation of aortic arch showed periventricular leukomalacia adjacent to the lateral ventrical. Case 7 (one-day old term infant) with midgut volvulus with extensive hemorrhagic necrosis of small bowel was not found to have significant neuropathological findings of HIE. Case 8 (1 day old female, born at 37 weeks) with hypoplastic left heart complex died shortly after birth and showed no evidence of white matter damage. Case 9 (four month old male born at 38 5/7 weeks) with congenital diaphragmatic hernia and hypoplastic aortic arch status post procedure was complicated by uncontrolled respiratory syncytial virus (RSV) pneumonia. Neuropathological evaluation of case 9 did not show diffuse white matter injury.

In vitro Oligodendrocyte Progenitor cell culture

OLPs were isolated by immunopanning postnatal day 7 CD-1 mouse cerebral cortices as previously described (Emery, B, et al. Cell 138(1): 172-185 (2009); Harrington, E, et al. Ann Neurol 68:703-16 (2010)). OLPs were plated on poly-D-lysine coated plates and coverslips and maintained in proliferation media in a 10% CO2 37°C incubator with 50% media changes every two days. Proliferation media has been described previously (Harrington, E, et al. Ann Neurol 68:703-16 (2010)). For differentiation, OPC proliferation media was replaced with differentiation media: OPC mitogens in proliferation media replaced with CNTF (10ng/ml) and T3 (40ng/ml).

XAV939 treatment of OLP cultures and differentiation assays

After immunopanning OLPs were plated at a density of 5,000 cells/coverslip in proliferation media and allowed to recover for 24 hours. XAV939 (Tocris) or DMSO was added to media for 24 hours. Differentiation was induced by replacement of media with differentiation media including pre-treatment XAV939 concentration or DMSO. For westerns OPCs were plated at a density of 0.5×106 cells/15cm plate and maintained until 75% confluent ~4 days. XAV939 or DMSO was added to media for indicated time period. For differentiation, cell counts of at least 3 coverslips and 3 separate experiments (n=9 and n=300-1000 cells for each test group) were imaged and counted. Comparison of means between two groups were analyzed with unpaired T-test and statistical significance was set at p<0.01.

Western Blot of OLP

Western blot was performed as previously described (Harrington, E, et al. Ann Neurol 68:703-16 (2010)). The primary antibodies were: beta-actin 1:5000 (Cell Signaling 4970), axin1 1:250 (Cell Signaling 2087), axin2 1:250 (Cell Signaling 2151), phospho-beta catenin 1:1000 (Cell Signaling 9561), tankyrase 1:1000 (Abcam ab13587), MBP 1:10,000 (Sternberger SMI-94).

XAV939 treatment in vivo in mouse spinal cord demyelination model

XAV939 (Tocris) was dissolved in DMSO to a concentration of 10mM, then diluted in sterile water to a concentration of 10μM. This stock was then added to 1% lysolecithin immediately before lesioning to a final concentration of 0.1μM XAV939. DMSO alone was added for control animals. Wild-type young adult mice (8-10 week old) were then co-injected with the mixture in dorsal or ventral funiculus spinal cord white matter as described above, and harvested at the times described post lesioning.

Expression profiling of Olig2cre/DA-cat vs WT spinal cord at P4

Whole spinal cords were harvested, homogenized in Trizol and then RNA was extracted using RNeasy kit (Qiagen). Microarray analysis was performed at the Genetics Core Facility, Gladstone Institute, San Francisco. For each of P4 WT and Olig2cre/DA-cat, RNA from three animals was pooled for use in each array chip, and three Affymetrix Mouse Genome 430 2.0 chips were run for each group, for a total of 9 animals for each group of WT and Olig2cre/DA-cat.

Ex vivo cerebellar slice cultures

Following decapitation, the brains of newborn CD1 wild-type mice (postnatal day 0-1) were dissected out into cold MEM (MEM supplemented with penicillin/streptomycin, Invitrogen) and L-Glutamine (4mM). Excess tissue was trimmed around the brain, ensuring that the cerebellum remained attached to the underlying piece of hindbrain. Using a McIlwain tissue chopper, 350μm sagittal slices of the cerebellum were cut and plated on Millicell-CM™ organotypic culture inserts (Millipore, 0.4μm) in slice culture medium containing 50% MEM with Earle’s salts, 35% Earle’s balanced salt solution, 25% heat-inactivated horse serum, glutamax, fungizone, penicillin-streptomycin (each from Invitrogen), and glucose (Sigma).

For the myelinating slice cultures, XAV or DMSO was added at the time of plating. Cultures were maintained at 37°C and 7.5% CO2. Membranes were transferred into fresh medium supplemented with tested factors every two days. The hypoxic slice cultures were exposed to 2% O2 conditions for 24h between 2-3 days in culture and then returned to normal culture conditions. XAV or DMSO was added following hypoxia exposure. Both the myelinating and hypoxic slices were fixed at 12 days in vitro. Remyelinating slice cultures were adapted from previous described methods (Birgbauer et al., 2004). After culturing slices in medium with no factors added for 14 days, demyelination was induced by the addition of 0.5% lysolecithin (Sigma) to the slice culture medium for 18 hours. Following demyelination, slices were then transferred to medium containing XAV or DMSO and maintained for an additional 14 days to allow remyelination to occur.

Immunostaining of slice cultures

Slices were immersed in 4%PFA for 1h while attached to membranes at the desired timepoints. Slices were then rinsed in PBS, blocked with 3% heat-inactivated horse serum, 2% bovine serum albumin, and 0.25% Triton X-100 in PBS, then incubated overnight at 4°C in primary antibody diluted in block solution. Primary antibodies were: chicken polyclonal anti-neurofilament 200kDa (NFH, Encor Biotech), rat monoclonal anti-MBP (Serotec), and rabbit polyclonal anti-Caspr (AbCam, 1:500).

Slice Culture imaging and quantification

Confocal z-stacks were acquired (12 slices) using Leica SP5 confocal microscope. Upon examination of staining, sections were chosen based on their intact cytoarchitechture and the formation of parallel myelinated tracts in the cerebellum. Any slices that did not meet these criteria were not analyzed. Imaging was focused on the areas of the cerebellum that contained parallel tracts of myelinated axons. Obtained images were processed and analyzed using the public domain NIH Image program (developed at the U.S. National Institutes of Health and available on Internet at http://rsb.info.nih.gov/nih-image/). Following compression of images into one z-stack, NIH Image was used to threshold area of staining for Caspr and NFH. Myelination was then quantified by a ratio of percent area stained for Caspr to percent area stained for NFH. All imaging and subsequent data analysis was done blinded to conditions being tested. Three independent experiments were conducted and analyzed. The data were analyzed by one-way ANOVA with Dunnett’s multiple comparison test (GraphPad Prism).

Supplementary Material

Acknowledgements

We thank Walter Birchmeier for Axin2-lacZ mice. This work was supported by a Promise 2010 grant from the National Multiple Sclerosis Society (to RJMF and DHR), the United Kingdom Multiple Sclerosis Society (to RJMF), the National Institute of Health (to DHR), and the Medical Scientist Training Program at the University of California, San Francisco (to EPH). SEB is a Harry Weaver Neuroscience Scholar of the NMSS. RN and DHR are a Howard Hughes Medical Institute Investigators.

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