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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Nat Med. Author manuscript; available in PMC 2012 May 1.
Published in final edited form as:
Published online 2011 April 24. doi:  10.1038/nm.2336
PMCID: PMC3140952

Ablation of Fmrp in adult neural stem cells disrupts hippocampus-dependent learning


Deficiency in fragile X mental retardation protein (FMRP) results in fragile X syndrome (FXS), an inherited form of intellectual disability. Despite extensive research, how FMRP deficiency contributes to the cognitive deficits in FXS is unclear. We have previously shown that Fmrp-null mice exhibit reduced adult hippocampal neurogenesis. Since Fmrp is also enriched in mature neurons, we explored the functional significance of Fmrp expression in neural stem and progenitor cells (aNSCs) and its role in adult neurogenesis. Here we show ablation of Fmrp in aNSCs via inducible gene recombination leads to reduced hippocampal neurogenesis in vitro and in vivo, as well as significantly impaired hippocampus-dependent learning in mice. Conversely, restoration of Fmrp expression specifically in aNSCs rescues these learning deficits. These data suggest that defective adult neurogenesis may contribute to the learning impairment seen in FXS, and these learning deficits can be rectified by delayed restoration of Fmrp specifically in aNSCs.


Although the specific purpose of adult neurogenesis is not entirely clear, evidence supports its important roles in adult neuroplasticity12. Ablating aNSCs via either mouse genetics or focal irradiation leads to deficits in hippocampus-dependent learning tasks38. Conversely, treatments that can enhance neurogenesis have a positive impact on hippocampus-dependent learning910. Both adult hippocampal neurogenesis and learning are altered in several pathological conditions12, but their involvement in human intellectual disability, a deficiency in learning and memory, remains elusive.

Fragile X syndrome (FXS), the most common form of inherited intellectual disability, is caused by the functional loss of fragile X mental retardation protein (FMRP)11. Patients with FXS have an array of deficits including impaired cognition and learning, yet their overall brain morphology shows only subtle changes12. Although Fmrp null (Fmr1 KO) mice perform relatively well in standard learning tests1315, they exhibit profound deficits in difficult association tasks16, particularly the hippocampus-dependent trace conditioning task1718. Fmrp is enriched in neurons therefore the learning deficits of FXS patients and mouse models are widely believed to result from a deficiency in the synaptic plasticity of mature neurons1921. We recently reported that Fmrp regulates aNSC fate, and the loss of functional Fmrp leads to impaired adult hippocampal neurogenesis22. However, whether Fmrp regulation of adult neurogenesis has any functional significance in learning remains a question.

In this study, we tested the hypothesis that Fmrp plays an important role in adult neurogenesis, and therefore hippocampus-dependent learning abilities. Using inducible conditional Fmrp deletion and restoration mouse lines, we show that selective deletion of Fmrp from aNSCs leads to impaired performance on two hippocampus-dependent learning tasks. Conversely, restoration of Fmrp specifically in aNSCs rescues these learning deficits. Therefore, our data offer direct evidence in support of a critical role for adult neurogenesis in hippocampus-dependent learning and reveal that defective adult neurogenesis contributes to the learning impairment seen in FXS. Remarkably, these learning deficits can be rectified by specific restoration of Fmrp in aNSCs of adult brains, which may lead to new therapeutic interventions for FXS.


Deletion of Fmrp from aNSCs alters hippocampal neurogenesis

To achieve targeted deletion of Fmrp specifically in aNSCs, we generated inducible Fmrp conditional knockout mice (Fmr1loxP/y:Nes-CreERT2:R26R-YFP or “cKO:Cre:YFP”) by crossing Fmrp conditional knockout (Fmr1loxP/y or “Fmrp cKO”) mice23 with inducible Nes-CreERT2 transgenic driver mice24 and Rosa26-stop-YFP (R26R-YFP) reporter mice (Supplementary Fig. S1). Tamoxifen (Tam) injection leads to efficient Cre:LoxP recombination in Nestin+ cells25. The male littermates, without the cKO allele (Fmr1+/y:Nes-CreERT2:R26R-YFP or “Cre:YFP Control”), were used as wild-type (WT) controls. At 1 day (d) post-Tam injection of adult mice, YFP+ cells could be found in the DG of both cKO:Cre:YFP and Cre:YFP Control mice, and more YFP+ cells could be found at 56 d post-Tam injections, indicating efficient Cre-mediated recombination (Fig. 1 and Supplementary Fig. S2). Fmrp expression was undetectable in the YFP+GFAP+ type 1 radial glia-like aNSCs at 1 d post-Tam (Fig. 1a) and remained absent at 56 d post-Tam (Supplementary Fig. S2a–c) in the cKO:Cre:YFP mice, compared to Cre:YFP Control mice (Fig. 1b and Supplementary Fig. S2d). Moreover, Fmrp was undetectable in both the YFP+doublecortin+ (YFP+DCX+) immature neurons (Supplementary Fig. S2e) and the YFP+NeuN+ mature neurons (Fig. 1c) of cKO:Cre:YFP mice, compared to Cre:YFP Control mice (Fig. 1d and Supplementary Fig. S2f). Therefore, Tam injection leads to Fmrp deletion specifically in aNSCs and in their progenies.

Figure 1
Fmrp deletion in Nestin-expressing cells resulted in fewer YFP+ cells in the DG. (a, b) Immunohistological analyses of brain sections from cKO:Cre:YFP mice (a) and Cre:YFP Control mice (b) at 1 d post-Tam. Red, Fmrp; green, YFP; white, GFAP; blue, DAPI. ...

To determine the effects of Fmrp ablation from Nestin-expressing cells on adult DG neurogenesis, we assessed the number of YFP+ cells and their phenotypes in cKO:Cre:YFP mice compared with Cre:YFP Control mice at 1, 14, 21, 28 and 56 d post-Tam (Supplementary Fig. S3a), representing critical developmental stages of new DG neurons in the adult1. The cKO:Cre:YFP mice had a significant lower number of YFP+ cells in general, compared to Cre:YFP Control mice (Fig. 1e, f, genotype F1,40 = 15.2, p < 0.0001). While the number of YFP+ cells in the DG of Cre:YFP Control mice showed a continuous increase from 1 to 56 d post-Tam, the number of YFP+ cells in cKO:Cre:YFP mice decreased initially until 14 d post-Tam before increasing (Fig. 1f, genotype x day F4,40 = 6.68, p < 0.0001). This reduction in the number of YFP+ cells did not affect the overall volume of the DG (Supplementary Fig. S3b, c) and was not a result of increased apoptosis of YFP+ cells (Supplementary Fig. S3d–f), as determined at 56 d post-Tam. Therefore, although more aNSCs were generated in the absence of Fmrp, fewer of these cells survived beyond 14 d post-Tam.

We next performed fate mapping of YFP+ cells in the SGZ (Fig. 2a–d). We used GFAP and S100β to distinguish the type 1 radial glia-like aNSCs (GFAP+S100β) from astrocytes (GFAP+S100β+)26. At 1 and 7 d post-TAM, there was no difference in the number of GFAP+S100β aNSCs between genotypes (Fig. 2e). However, starting from 14 d post-TAM, the number of YFP+GFAP+S100β aNSCs was 21–32% higher in cKO:Cre:YFP mice compared with Cre:YFP Control mice (Fig. 2f, genotype F1,40 = 5.6, p = 0.023). Using Ki67 as a marker for proliferating cells, we found that the numbers of YFP+Ki67+(DCX) immature cells (type 1 aNSCs + TAP cells) were higher in cKO:Cre:YFP mice compared with Cre:YFP Control mice from 14 d post-Tam and beyond (Fig. 2g, genotype F1,40 = 11.99, p = 0.001). Therefore, the deletion of Fmrp from Nestin-expressing aNSCs leads to increased production of aNSCs and immature cells, which is consistent with our published observation in Fmr1 KO mice22.

Figure 2
Selective deletion of Fmrp in Nestin-expressing cells alters cell proliferation and fate specification of aNSCs. (a) Schematic diagram showing the cell lineage-specific markers across stages of neurogenesis, which were used for fate mapping. (b–d ...

Young neurons in the DG first express DCX from 3 d post-cell cycle exit. As the neurons become mature, starting from ~2 weeks post-cell cycle exit, they lose expression of DCX but gain expression of a mature neuronal marker, NeuN1. Consistent with litareature25, we observed that ~20% of DCX+ cells was proliferating (DCX+Ki67+ neuroblasts), whereas the majority of DCX+ cells were post-mitotic (DCX+Ki67 immature neurons). We found that the numbers of neuroblasts (Fig. 2g, F1,40 = 36.1, p < 0.0001), and to a less extent, post-mitotic young neurons (Fig. 2h, F1,40 = 25.32, p < 0.0001) were both reduced in cKO:Cre:YFP mice compared with Cre:YFP Control mice at as early as 7 d post-Tam. Therefore reduced proliferation of neuroblasts may underlie the reduced DCX+ cells in cKO:Cre:YFP mice. Since new cells needed 3–4 weeks to reach the NeuN+ mature stage, the differences between genotypes became significant at 28 d post-Tam (31% reduction), and more profound at 56 d (45% reduction) post-Tam (Fig. 2i genotype × day F4,40 = 20.7, p < 0.0001). Furthermore, this reduction of NeuN+ neurons contrasted with the increase in the number of S100β+GFAP+ astrocytes at day 28 (30% increase) and day 56 (50% increase) post-Tam (Fig. 2j, genotype x day F4,40 = 3.5, p = 0.015). The changes in the percentage of each cell lineage among YFP+ cells closely resembled the numbers of each cell lineage in the DG (Supplementary Fig. S4), suggesting that the altered numbers of each lineage type in cKO:Cre:YFP mice were largely due to the changes in fate specification of YFP+ cells. Thus, selective deletion of Fmrp in Nestin+ cells leads to increased proliferation, decreased neuronal differentiation and increased astrocytic differentiation in vivo, consistent with our previous report22. These phenotypic changes result from a cell-autonomous effect of Fmrp-deficiency in aNSCs.

Because Fmrp is known to regulate the maturation and synaptic plasticity of neurons27, we analyzed the dendritic morphology of YFP+ neurons at 56 d post-Tam (Supplementary Fig. S5a–f). The YFP+ newborn neurons in cKO:Cre:YFP mice exhibited significant reductions in dendritic complexity as assessed by Sholl analysis (F1,38 = 11.967, p = 0.001), total dendritic length (p < 0.05), number of branching points (Nodes, p < 0.05), and number of dendritic ends (p < 0.05). Therefore, Fmrp expression in aNSCs is critical for multiple stages of neurogenesis.

Fmrp deletion alters aNSC proliferation and differentiation

To further confirm the cell-autonomous function of Fmrp in aNSCs, we isolated aNSCs from the DG of Fmrp cKO mice (Fmr1loxP/y) and WT littermates (Fmr1+/y). These primary DG-aNSCs possessed the same essential properties of NSCs as demonstrated previously22. Infection by retrovirus expressing a Cre-GFP fusion protein resulted in marked reduction of Fmrp expression in cKO cells, but not in WT cells (Supplementary Fig. S5g). While cKO cells without Cre exhibited no differences in either proliferation (Fig. 3a, b-left) or differentiation (Fig. 3c, d-left, e-left) compared with WT cells, Cre-GFP virus-infected cKO cells exhibited a 29% increase in BrdU incorporation (Fig. 3b-right, n = 3, p < 0.05), a 40% decrease in neuronal differentiation (Fig. 3d-right, n = 3, p < 0.05), and a 39% increase in astrocyte differentiation (Fig. 3e-right, n = 3, p < 0.05) compared with virus-infected WT cells. These results are consistent with our previous findings in Fmr1 KO aNSCs22.

Figure 3
Selective deletion of Fmrp in primary aNSCs isolated from adult DG results in altered proliferation and differentiation of aNSCs and reduced neurite extension of aNSC-differentiated neuron. (a, b) Proliferation analysis. (a) Sample image of aNSCs with ...

Furthermore, compared with virus-infected WT aNSCs, Cre-GFP virus-infected cKO aNSCs differentiated into neurons with reduced neurite complexity as assessed by Sholl analysis (Fig. 3g, WT+Cre vs. cKO+Cre F1,38 = 8.993; p = 0.005), neurite length (Fig. 3h, p < 0.05), number of branching nodes (Fig. 3i, p < 0.01), and number of ends (Fig. 3j, p < 0.05). Therefore, Fmrp has important cell-autonomous functions at multiple stages of adult neurogenesis.

Deletion of Fmrp from aNSCs leads to impaired learning

Hippocampal neurogenesis is believed to be involved in hippocampus-dependent learning12. We hypothesized that Fmrp deletion-induced neurogenesis deficits would have a negative impact on hippocampus-dependent learning. Since Fmr1 KO mice exhibit deficits in challenging learning tasks16 and particularly the trace conditioning task1718 that require hippocampal neurogenesis10,28, we decided to use this task to assess learning (Supplementary Fig. S6a, b). We first tested Fmr1 KO mice and found they exhibited significantly less trace learning as shown by reduced freeze to both the training context (Fig. 4a, F1,9 = 267, p < 0.001) and to the tone (Fig. 4b, F1,9 = 92, p < 0.001) compared with WT littermates. Then we tested the cKO:Cre:YFP mice at 30 d post-Tam injection (Supplementary Fig. S6a). cKO:Cre:YFP mice exhibited significantly reduced freezing behavior in both the context test (Fig. 4c, F1,12 = 55, p < 0.001) and tone test (Fig. 4d, F1,12= 50, p < 0.001) compared with Cre:YFP Control mice. We saw no effect of Tam on these behaviors (Supplementary Fig. S7a, b).

Figure 4
Deletion of Fmrp from Nestin-positive aNSCs results in hippocampus-dependent learning deficits. (a, b) Context (a) and Tone (b) trace learning analyses of Fmr1 KO mice and WT control littermates as determined by the percentage of the time that the animal ...

Since adult hippocampal neurogenesis may impact the ability of animals to discriminate between similar stimuli3,9, we tested mice in a delayed nonmatching-to-place radial arm maze (DNMP-RAM) task designed to assess hippocampus-dependent spatial pattern separation3 (Supplementary Fig. S6a, c). We first confirmed that Fmrp deficiency had no effect on olfaction using a buried food test (Supplementary Fig. S7c). We then tested the performance of Fmr1 KO mice on this DNMP-RAM task. Fmr1 KO mice performed significantly worse in both test settings, particularly in separation 2, compared with WT mice (Fig. 4e; genotype F1,18= 12.4, p < 0.002). Similarly, cKO:Cre:YFP mice performed significantly worse in both separation settings compared with Cre:YFP Control littermates (Fig. 4f; genotype F1,24 = 6.1, p < 0.0001). On the other hand, neither Fmr1 KO mice nor Tam-injected cKO:Cre:YFP mice exhibited deficits in tasks that do not require hippocampal neurogenesis, such as olfactory discrimination task for assessing olfactory learning and a novel object recognition task for assessing visual short-term memory (Supplementary Fig. S7d–f, S8a–b). Although anxiety has been reported in Fmr1 KO mice under certain genetic backgrounds17,2930 which could potentially affect behavioral results, we observed no increased anxiety in either Fmr1 KO or Tam-injected cKO:Cre:YFP mice (Supplementary Fig. S8c–d). Therefore, selective deletion of Fmrp from aNSCs leads to specific deficits in highly challenging learning tasks that depend on adult hippocampal neurogenesis.

Restoration of Fmrp in aNSCs rescues learning

Next we test the hypothesis that restoration of Fmrp would rescue neurogenesis by using a Fmrp conditional restoration mouse line (Fmr1loxP-Neo/y or Fmrp cON). These mice express normal levels of Fmrp only after Cre-mediated deletion of the inserted Neo gene (Supplementary Fig. S1c). Indeed, Cre-GFP retrovirus infection could restore Fmrp expression in aNSCs isolated from Fmrp cON mice (Supplementary Fig. S5h). Without Cre-GFP virus-mediated recombination, Fmrp cON aNSCs exhibited a 30% higher proliferation rate (Fig. 5a, b-left, p < 0.05), 40% less neuronal differentiation (Fig. 5c, d-left, p < 0.05), and 40% more astrocyte differentiation (Fig. 5c, e-left, p < 0.05), compared with WT control aNSCs. Furthermore, these Fmrp cON aNSCs differentiated into neurons with reduced neurite complexity as assessed by Sholl analysis (Fig. 5f, g, WT vs. cON F1,26 = 18.077; P < 0.001), neurite length (Fig. 5h, p < 0.05), number of nodes (Fig. 5i, p < 0.01), and number of ends (Fig. 5j, p < 0.05). Therefore, Fmrp cON aNSCs without Cre exhibit deficits that were similar to those seen in Fmr1 KO aNSCs22 and Cre virus-infected Fmrp cKO aNSCs (Fig. 3). Importantly, Cre-mediated restoration of Fmrp in aNSCs rescued the proliferation, differentiation, and neurite extension deficits of cON cells (Fig. 5b-right, 5d-right, 5e-right, 5g-j), demonstrating that selective restoration of Fmrp expression in aNSCs is capable of rescuing the deficits of Fmrp-deficient aNSCs.

Figure 5
Restoration of Fmrp in primary aNSCs rescues proliferation and differentiation deficits of Fmrp-deficient aNSCs and neurite extension deficits of aNSC-differentiated neurons. (a, b) Proliferation analysis. (a) Sample image of aNSCs with (+) or without ...

We then assessed whether restoration of Fmrp expression specifically in aNSCs could rescue learning deficits. We generated inducible conditional Fmrp restoration (Fmr1loxP-Neo/y:Nes-CreERT2:R26R-YFP or “cON:Cre:YFP”) mice by crossing Fmrp cON mice with Nes-CreERT2:R26R-YFP mice24. We designed a specific breeding strategy (Supplementary Fig. S1d) that allowed us to generate cON:Cre:YFP mice together with two essential littermate controls: Fmr1+/Y:Nes-CreERT2+/−:R26R-YFP+/− (Cre:YFP Control) mice that expressed functional Fmrp and Fmr1loxP-Neo/Y:R26R-YFP+/− (cON:YFP Control) mice that did not express functional Fmrp, either before or after Tam injection and all mice received Tam injections. At 28 d post-Tam, Fmrp expression was restored only in Nestin+ aNSCs and their subsequent progenies of the cON:Cre:YFP mice (Fig. 6a, b).

Figure 6
Restoration of Fmrp in Nestin-expressing aNSCs and their progenies rescues hippocampus-dependent learning deficits. (a, b) Immunohistological analyses of brain sections from cON:Cre:YFP mice at 56 d post-Tam. (a) Red, Fmrp; green, YFP; white, GFAP; blue, ...

We found that cON:YFP Control mice, without functional Fmrp, exhibited deficits in both context trace learning (Fig. 6c, F1,10 = 93, p < 0.0001) and tone trace learning (Fig. 6d, F1,10 = 85, p < 0.0001). On the other hand, the Tam-injected cON:Cre:YFP mice performed significantly better compared with cON:YFP Control littermates in both context trace learning (Fig. 6c, F1,10 = 1665, p < 0.0001) and tone trace learning (Fig. 6d, F1,10 = 88, p < 0.0001). Similar results were obtained for DNMP-RAM tests. The cON:YFP Control mice performed poorly in both separation 2 and separation 4 tests compared with Cre:YFP Controls (Fig. 6e. genotype p < 0.01), reminiscent of Fmr1 KO mice and cKO:Cre:YFP mice (Fig. 3). However cON:Cre:YFP mice performed similarly as Cre:YFP Controls (Fig. 6e, t (12) = 0.57, ns). Therefore, restoration of Fmrp specifically in adult aNSCs and their progenies rescue these two hippocampus-dependent learning deficiencies.


In this study we manipulated the intrinsic properties of aNSCs in adult brains both positively and negatively without changing the surrounding niche cells or causing significant aNSC death. Our data suggest that the loss of functional FMRP in aNSCs may contribute to the cognitive deficits seen in FXS patients.

Earlier studies have found inconsistent hippocampus-dependent learning deficits in Fmr1 KO mice14,3132; however, we saw robust behavioral changes, likely for two reasons. First, we minimized variability that might affect behavioral tests by using only littermate males and by performing all tests during the dark cycle when mice were active. Second, we designed tasks that were more challenging than standard tests1618. “Trace conditioning” is more sensitive for detecting hippocampal dysfunctions1718,3335, than the standard “delay fear conditioning” used by others14,3132. The DNMP-RAM is also a much more challenging task compared with standard radial arm maze3.

The role of adult neurogenesis in learning and memory has long been debated. One theory is that adult DG neurogenesis is important for fine spatial distinctions3,3638. Our DNMP-RAM data support this theory. Another theory proposes that adult neurogenesis is important for clearance of memories from the hippocampus and into storage in the cortex thereby maintaining the capacity of the hippocampus for establishing new memories10,3940. Although not addressed directly in our current experiments, our model certainly lays the groundwork to test this theory in the future. It is possible these theories to some extent underestimate the significance of adult neurogenesis in hippocampal function, which becomes more apparent when aNSCs are altered rather than deleted. In Clelland et al3., ablation of neurogenesis only affected the performance of mice on difficult tasks. Our Tam-injected cKO:Cre:YFP mice with partial reduction of neurogenesis were impaired in both easy and difficult tasks. It is possible that Fmrp deficiency may have a more profound negative effect on adult neurogenesis and learning than irradiation. Synaptic competition between old and new neurons is known to occur when newborn neurons form synaptic connections with preexisting boutons in the DG41. Newly generated neurons have transiently enhanced synaptic plasticity4243; therefore, they may have a strong transient ability to deprive preexisting synapses. New Fmrp-deficient neurons may therefore form defective synapses and interfere with existing neural networks, thereby disrupting the learning of even those easier tasks.

Therefore, Fmrp deficiency in aNSCs may impact adult hippocampus-dependent learning through several aspects, including reduced production of new DG neurons; disruption of hippocampal circuitry by introducing abnormal new DG neurons; and overproduction of astrocytes due to altered differentiation of aNSCs. Which aspect of Fmrp’s regulation of adult neurogenesis contributes to the learning deficits is the next question that needs to be answered. Although altered embryonic neurogenesis in Fmrp-deficient mice and humans has been reported4447, the involvement of adult neurogenesis is a previously unrecognized facet in the etiology of FXS, which could point to possible new treatments for this disease. Hence, in addition to altered synaptic plasticity of mature neurons, defective neurogenesis in postnatal and adult brains could also contribute to the pathogenesis of intellectual disability in adult humans.


Detailed methods and any associated references are available in the online version of the paper at (Detailed Methods are provided in the “Supplementary Data and Methods”):


We performed all procedures involving live animals according to protocols approved by the University of New Mexico Animal Care and Use Committee. We generated the inducible conditional mutant and restoration mice by breeding Nes-CreERT2+/+: R26R-YFP+/+ homozygote male mice24 with female Fmr1loxP/y mice23 and female Fmr1loxP-Neo/yNes-CreERT2+/−:R26R-YF−/− mice24, respectively We genotyped the mice as previously described 2324,48. Tamoxifen (Sigma-Aldrich) was performed based on a published procedure 24.

In vivo cell fate mapping

For In vivo fate mapping of YFP+ cells, we euthanized the mice at 1, 7, 14, 28 or 56 d after the last TAM injection, by intraperitoneal injection of sodium pentobarbital and then transcardially perfused with saline followed by 4% PFA. We performed histological analysis of mouse brains as described previously with modifications22,24,49. For quantification of YFP+ SGZ cells, we used 1 in 12 serial sections starting at beginning of hippocampus (relative to bregma, − 1.5 mm) to the end of hippocampus (relative to bregma, − 3.5 mm). We determined the YFP+ cells in the granule layer and the volume (3-dimentional size) of the DG using unbiased stereology (StereoInvestigator, MBF Biosciences, Inc)22,49. We performed phenotypic analysis of YFP+ cells as described24. We analyzed the dendritic complexity, including Sholl analysis, dendritic length, number of branches, and number of ends, using Neurolucida8 (MBF Bioscience, Inc) as described in our publication50.

Isolation and analyses of aNSCs

We isolated aNSCs from the DG of 8 to 10-week-old male mice based on published methods22,51. We maintained aNSCs and carried out cell proliferation and differentiation analyses as described 22,5253. H van Pragg and F Gage cloned the sequence for Cre-GFP fusion protein from a published adeno associate viral vector54 into a retroviral vector. We produced retrovirus using the method described in our previous publications4950,53. For conditional deletion or restoration of Fmr1 genes in aNSCs, we added Cre-GFP retroviruses twice (once per day) under proliferation condition for 2 d before the initiation of proliferation or differentiation assays. We routinely obtained ~100% infection efficiency. We determined the efficiency of Cre-induced recombination by using Western blotting with an anti-Fmrp antibody (1:500, Millipore). For loading controls, we were stripped and reprobed the membranes with the antibody against β-Actin (1: 1000, Sigma).

Behavioral Analyses

We performed these tests based on published methods. Trace Conditioning Tests were performed using a Coulbourn Habitest fear conditioning system as described1718,55 with modifications. DNMP-RAM test was performed using a Coulbourn Instruments radial arm maze based on published method3. Each day mice received 2 trials (sample + choice phase) for each of the two separations (separation 2 and separation 4) per day for 5 consecutive days. Repeated measures ANOVA between group and separations were carried out using SPSS for each experiment Post hoc Student’s t-tests with Bonferroni corrections were used as needed. Object Recognition Test, Buried Food Test, and Elevated Plus Maze Test were performed based on our published papers5658. Data are analyzed using ANOVA (SPSS version 18, SPSS Inc.)

Statistical Analyses

Unless specified otherwise, statistical analyses were performed using unpaired, two-tailed, Student’s t-test or ANOVA. The data bars and error bars indicate mean ± standard error mean. (s.e.m). Scholl analysis was analyzed using multivariate analysis of variance (MANOVA) using SPSS statistical software.

Supplementary Material

Sup Info


We would like to thank C.T. Strauss for editing the manuscript, H. van Praag, D. Schaffer, and T. Xie for critical reading of the manuscript, members of the Zhao Lab for helpful discussions, S. J. von Hoyningen-Huene for technical assistance, D. Lagace and R. Smrt for technical help, and H. van Praag (NIH/NIA) and F.Gage (Salk Institute) for providing Cre-GFP construct. This work was supported by grants from the US NIH to XZ (MH080434 and MH078972) and DLN (HD38038 and HD024064). EGS is supported by an NIH/NIMH Career Opportunity for Research (COR) training grant (MH19101).



W.G. and X.Z. planned the experiments, analyzed data, and wrote the manuscript. A.M.A. developed, carried out and analyzed data for behavioral analyses. W.G., L.Z., E.B.J., E.G.S., A.C.M., S.L.G. and A.M.A. carried out all the experiments. P.J. helped with mouse line acquisition and concept development. R.Z., B.A.O., and D.L.N. made Fmr1 cON mice. A.J.E. provided Nes-CreERT2 mouse line and ROSA26-YFP mouse line and guided histological analysis.


The authors declare no competing financial interests.


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