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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
J Neurosci. Author manuscript; available in PMC 2012 January 6.
Published in final edited form as:
PMCID: PMC3139999

A role for nyctalopin, a small leucine rich repeat protein, in localizing the TRPM1 channel to retinal depolarizing bipolar cell dendrites


Expression of channels to specific neuronal sites can critically impact their function and regulation. Currently, the molecular mechanisms underlying this targeting and intracellular trafficking of TRP channels remains poorly understood and identifying proteins involved in these processes will provide insight into underlying mechanisms. Vision is dependent on the normal function of retinal depolarizing bipolar cells (DBCs), which couple a metabotropic glutamate receptor 6 (mGluR6) to the TRP melastatin 1 (TRPM1) channel to transmit signals from photoreceptors. We report that the extracellular membrane attached protein, nyctalopin, is required for the normal expression of TRPM1 on the dendrites of DBCs in mus musculus. Biochemical and genetic data indicate that nyctalopin and TRPM1 interact directly suggesting that nyctalopin is acting as an accessory TRP channel subunit critical for proper channel localization to the synapse.

Keywords: TRPM1, nyctalopin, depolarizing bipolar cell, dendritic targeting, retina


Being in the right place at the right time is fundamental for signaling proteins. Though the importance of regulating the spatial distribution of signaling proteins is well recognized, the proteins mediating this coordinated process remain largely unknown. This is especially true for the transient receptor potential (TRP) superfamily of ion channels. Although a few TRP channels have been shown to interact with cellular components involved in trafficking, such as cytoskeletal or vesicular proteins, specific accessory proteins required for TRP channel cellular localization have not been identified.

TRPM1 is the initial member of the melastatin TRP sub-family and was identified in a screen analyzing mRNA levels in malignant verse benign melanocytes (Duncan et al., 1998). TRPM1 in melanocytes is now believed to play a largely intracellular role, possibly regulating melanin production (Oancea et al., 2009; Patel and Docampo, 2009). Recently, TRPM1 was identified in the retina, where it is required for light driven depolarizing bipolar cell (DBC) responses (Koike et al., 2010). These results established that TRPM1 also functions in the plasma membrane.

The question of whether TRPs have intrinsic targeting information encoded in their sequence or whether their residence in the plasma membrane is mediated by extrinsic proteins remains open. The dichotomy of TRPM1 channel localization in the intracellular membranes of melanocytes versus dendritic tips of DBCs suggests that extrinsic factors, specific to each cell type, are likely influencing TRPM1 localization and ultimately function. With this in mind, a protein expressed exclusively on the DBC dendritic tips and critical for generating a TRPM1 response is a strong candidate for regulating TRPM1 targeting to the plasma membrane.

Retinal DBCs detect changes in photoreceptor glutamate release via the metabotropic glutamate receptor, mGluR6. Glutamate activates mGluR6 in the dendrites, which results in closure of TRPM1. Mutations in either mGluR6 or TRPM1 in humans results in congenital stationary night blindness (Audo et al., 2009; Li et al., 2009; Nakamura et al., 2010; van Genderen et al., 2009). In mouse, primates, and humans TRPM1 is localized in the dendritic tips of DBCs (Koike et al., 2010; Morgans et al., 2009; van Genderen et al., 2009) and some mutations prevent TRPM1 delivery to the DBC dendrites (Nakamura et al., 2010). The small leucine-rich repeat protein, nyctalopin, is critical for generating a DBC response because spontaneous mutations in the Nyx gene are linked to night blindness in humans and mice (Bech-Hansen et al., 2000; Gregg et al., 2003). In a nyctalopin mutant mouse, Nyxnob, the DBCs do not have a glutamate response, which is believed to be due to either malfunction of the TRPM1 channel or its absence from the dendrites (Gregg et al., 2007).

In this study we used genetic, biochemical and immunohistochemical approaches to show that nyctalopin directly interacts with TRPM1 and that the lack of nyctalopin leads to an absence of TRPM1 from the dendrites of DBCs. These data indicate that nyctalopin is required for localization of TRPM1 to the dendritic tips of DBCs.

Materials and Methods


All experiments were performed using protocols approved by the Animal Care and Use Committee at each institution, and the guidelines of the National Institutes of Health and the Society for Neuroscience. Mice of either sex were used in experiments. The Nyxnob mice, originally discovered on the BALBc/ByJ background (Pardue et al., 1998), were back-crossed onto the C57Bl/6J background for more than seven generations. Controls were either littermates or age-matched C57Bl/6J mice (The Jackson Laboratory, Bar Harbor, ME). Trpm1−/− mice were generated by Lexicon Genetics (Trpm1tm1Lex) and obtained from the European Mouse Mutant Archive. Molecular details of the targeted allele are available at Transgenic mice expressing a YFP-nyctalopin in retinal bipolar cells were generated as previously described (Gregg et al., 2007) and will be referred to as TgEYFP-NYX mice. Mice were housed in a 12:12 light:dark cycle and killed by anesthetic overdose or carbon dioxide exposure.

Plasmid Construction

All cloning utilized the infusion cloning system (Clonetech, Mountain View, CA). PCR of full length Trpm1 was performed using retinal cDNA generated using Superscript III (Invitrogen, Carlsbad, CA) and total RNA isolated from C57Bl/6J retinas. A flag-tag (5’-GACTACAAGG ACGACGAC-3’) followed by GFP cDNA that was fused to the 5’ end of a full length TRPM1 cDNA. For expression of a tagged nyctalopin Strep-tag II (5’-TGGAGCCACCCGCAGTTCGAAAAG-3’) was fused to EYFP and both inserted after amino acid 19 of the nyctalopin. Both fusion constructs, Flag-GFP-TRPM1 (FG-TRPM1) and Strep-tagII-EYFP-nyctalopin (SY-NYX) were inserted into the EcoR1 site of pcDNA3.1+ (Invitrogen, Carlsbad, CA).

Membrane Yeast Two-Hybrid (MYTH)

Full length Nyx cDNA was cloned into the pCCW-SUC bait vector (Dualsystems Biotech), such that the C-terminal ubiquitin moiety and a LexA-VP16 transcription factor were fused to either the N- or C-terminus to create Cub-NYX and NYX-Cub, respectively. Trpm1 cDNA was cloned into the pDLS-Nx prey vector. Interactions were tested by co-transformation of bait and prey vectors into NYM32 yeast. Incorporation of both plasmids was tested by growth on plates lacking leucine and tryptophan (double dropout) and interaction by growth on plates lacking leucine, tryptophan, histidine and adenine (quadruple dropout). Interactions were confirmed by using a colony lift assay that tests for expression of β-galactosidase and was performed as previously described (Iyer et al., 2005).

Strep-tactin Magnetic Bead Pull Down

HEK293T (human embryonic kidney cells; ATCC, Manassas, VA) cells were grown in high glucose DMEM supplemented with 10% fetal bovine serum, L-glutamine (2 mM), penicillin (50 IU/ml) and streptomycin (50 µg/ml). Two 10 cm plates were transiently transfected using the CalPhos Mammalian Transfection Kit (Clonetech, Mountain View, CA). Cells were harvested by sonicating in 400 µl NP-T lysis buffer (50 mM NaH2PO4, 300 mM NaCl, 0.05% Tween-20, pH to 8.0 with 1 M NaOH). Homogenate cleared by centrifuging at 15,000 × g for 10 minutes at 4°C. 40 µl of total lysate was saved, while the remaining was incubated with 200 µl of strep-tactin magnetic beads (Qiagen, Valencia, CA) on a rocker at 4°C for 2–3 hrs. Beads washed three times in NP-T Lysis buffer before proteins eluted with 10 mM D-Biotin (Sigma Aldrich, Saint Louis, MO) in NP-T Lysis buffer. SDS sample buffer (62 mM Tris, 10% glycerol, 2% SDS, and 5% β-mercaptoethanol) was added and samples were incubated for 10 minutes at 70°C before western blotting.

Western Blotting

Protein fractions were analyzed on 4–12% NuPAGE gels (Invitrogen, Carlsbad, CA) and proteins were transferred onto PVDF membranes (GE Healthcare, Piscataway, NJ). Immunoblot analysis was performed using primary antibodies; anti-GFP (1:2000, Cell Signaling, Danvers, MA), anti-Flag (1:2000, Sigma Aldrich, Saint Louis, MO) and anti-TRPM1 (1:500, Koike et al., 2010). Anti-mouse (1:100,000, Sigma Aldrich, Saint Louis, MO) and anti-Rabbit (1:200,000, Pierce, Rockford, IL) HRP-conjugated secondary antibodies were used to detect bands with the advanced ECL detection system (Pierce, Rockford, IL).

Retinal Preparation & Immunohistochemistry

Retinal sections, whole mounts and immunohistochemistry were performed as described previously (Gregg et al., 2007). Antibodies and their dilutions are as follows: anti-GFP conjugated to Alexa-488 (1:1000, Invitrogen, Carlsbad, CA), anti-ctbp2/ribeye (1:1000, BD Biosciences Pharmingen, San Diego, CA), anti-Na+/K+-ATPase (1:300, Santa Cruz Biotechnology, Santa Cruz, CA), and anti-TRPM1 (1:100, Koike et al., 2010). Secondary antibodies were Alexa-488 goat anti-rabbit, Alexa-555 goat anti-mouse and Alexa-643 conjugated PNA (Invitrogen, Carlsbad, CA) all used at the 1:1000 dilution. Images were collected using the Olympus FV300 confocal microscope with 60X oil objective (1.45 NA). Images shown are maximum projections of confocal stacks, adjusted for contrast and brightness with Fluoview software.

Patch clamp recordings

Retinal slices from 4–6 week old C57Bl/6J and Nyxnob mice were collected for patch clamp recordings as previously described (Shen et al., 2009; Snellman and Nawy, 2004). Picrotoxin (100 µM), strychnine (10 µM) and 1,2,5,6-Tetrahydropyridin-4-yl) methylphosphinic acid (TPMPA, 50 µM) were included in all experiments to block inhibitory conductances. The metabotropic receptor antagonist LY341495 (Tocris Bioscience, Ellisville, MO), or TRP channel agonist Capsaicin (Sigma Aldrich, Saint Louis, MO) were delivered to the retina from a pipette using positive pressure (2–4 PSI) with a computer-controlled solenoid valve (Picospritzer, General Valve Corp, Fairfield, NJ), and the mGluR6 agonist L-AP4 (4 µM, Tocris Bioscience, Ellisville, MO) was added to the bath. Whole-cell recordings were obtained as previously described (Shen et al., 2009; Snellman and Nawy, 2004). Data were analyzed offline with Axograph X and Kaleidagraph (Synergy Software, Reading, PA). Holding potential for all cells was +40 mV.


TRPM1 currents are absent from DBCs lacking nyctalopin

In mice lacking nyctalopin, Nyxnob, the DBCs do not respond to glutamate (Gregg et al., 2007). We employed whole cell patch clamp to further examine TRPM1 currents in Nyxnob DBCs. In wildtype DBCs, the application of the mGluR6 antagonist, LY341495, inactivates the mGluR6 mediated cascade resulting in closure of TRPM1 and generation of an outward current (Fig. 1A, Shen et al., 2009). Consistent with our previous data, LY341495 failed to induce a current in rod DBCs from the Nyxnob mice (Fig. 1A, Gregg et al., 2007). Application of capsaicin, which has been shown to directly gate the DBC channel (Shen et al., 2009), was then used to test if any functional TRPM1 remained in the membrane in the absence of nyctalopin. In wildtype cells, application of capsaicin produces a robust outward current similar to LY341495 (Fig. 1B). However, this current was absent from rod DBCs in Nyxnob mice (Fig. 1B). As a negative control, DBC recordings were performed in TRPM1−/− mice and show no response to either LY341495 or capsaicin (Fig. 1A and 1B). The average peak response to LY341495 and capsaicin for each mutant is summarized in Fig. 1C. These data indicate that in the Nyxnob mice TRPM1 channels responsive to capsaicin are absent from DBC plasma membranes.

Fig. 1
Rod bipolar cells in Nyxnob mice lack functional TRPM1 channels

TRPM1 localization to DBC dendrites is dependent on nyctalopin

Given the patch clamp data, we examined the relationship between nyctalopin and TRPM1 in the OPL to determine whether the TRPM1 channel was correctly localized in the Nyxnob mice. TRPM1 is expressed on the DBC somas and as puncta on the dendritic tips of human, mouse and monkey DBCs (Koike et al., 2010; Morgans et al., 2009; van Genderen et al., 2009). To visualize nyctalopin in DBCs, we used a line of mice, TgEYFP-NYX, that express a functional EYFP-nyctalopin fusion protein in all DBCs (Gregg et al., 2007). Immunohistochemical staining of retinal cross-sections of TgEYFP-NYX mice show that EYFP-nyctalopin expression is restricted to DBC dendrites (Fig. 2A, Gregg et al., 2007) and TRPM1 also is expressed as discrete puncta on the dendritic tips of DBCs, and additionally in the DBC cell somas (Fig. 2A). The merged image shows that TRPM1 and nyctalopin colocalize at the characteristic synaptic puncta of DBCs (Fig. 2). The same co-localization pattern of EYFP-NYX and TRPM1was observed on the small rod DBC dendrites (arrows, Fig. 2B) and large cone DBC dendrites (arrowheads, Fig. 2B) in retina flat-mounts.

Fig. 2
The TRPM1 channel co-localizes with nyctalopin in DBC dendrites

To determine if the expression and localization of nyctalopin and TRPM1 to the tips of DBC dendrites was mutually dependent, we examined expression of TRPM1 in retinas from Nyxnob mice and nyctalopin in retinas from Trpm1−/−/TgEYFP-NYX mice. Both these mouse lines have normal retinal morphology (Ball et al., 2003, Koike et al., 2010; Morgans et al., 2009). We first analyzed the expression pattern of TRPM1in Nyxnob mice. Fig. 3 shows retinal cross-sections immunostained for TRPM1 (green) and peanut agglutinin (PNA, red), a marker for the cone synapses. In wildtype mice, TRPM1 colocalizes with PNA in a pattern consistent with expression on the tips of cone DBC dendrites (Fig. 3Ai, arrows). The small TRPM1 puncta observed above the cone terminals represent staining at the tips of rod BC dendrites (Fig. 3Ai). As previously shown, there was no TRPM1 staining in the retinas of Trpm1−/− mice (Fig. 3Aii, Morgans et al., 2009; Koike et al., 2010). Fig. 3Aiii shows that the characteristic punctate staining of TRPM1 in the OPL of wildtype mice is absent in the Nyxnob retinas. The extent of colocalization of TRPM1 and PNA on cone terminals was quantified by measuring fluorescence intensity in the green and red channels (Fig. 3B). These data reinforce the qualitative data for the images, namely that TRPM1 expression in the OPL is dependent on nyctalopin expression.

Fig. 3
The TRPM1 channel is absent from DBC dendrites in Nyxnob mice

Interestingly, in the absence of nyctalopin TRPM1 staining remains in the DBC cell bodies. To determine the localization of this staining, either in the plasma or biosynthetic membranes, we co-labeled for TRPM1 and Na+/K+-ATPase, the latter of which is a marker for the plasma membrane. The merged image of TRPM1 and Na+/K+-ATPase show robust staining of TRPM1 (green), indicating there is little co-localization (Fig.3C). The staining for Na+/K+-ATPase in wildtype mice was indistinguishable from that in Nyxnob mice (data not shown). These results indicate that the TRPM1 remaining in the Nyxnob DBCs is located in biosynthetic membranes. This location also is consistent with the patch clamp data showing a lack of functional TRPM1 channels in the Nyxnob DBC plasma membranes (Fig. 1B). To determine if the decrease in TRPM1 expression in the OPL decreased overall levels of TRPM1 we used quantitative western blotting and qRT-PCR. Western blot analyses of TRPM1 in whole retina lysates from wildtype, Nyxnob, and Trpm1−/− mice show that the level of TRPM1 is decreased in Nyxnob animals (Fig. 4). The level of TRPM1 mRNA in the Nyxnob retinas was not different from controls (data not shown), arguing that the reduction in protein expression is a posttranscriptional effect.

Fig. 4
TRPM1 protein levels are reduced in Nyxnob mice

To examine whether nyctalopin expression is dependent on the presence of TRPM1 we examined the expression pattern of nyctalopin in Trpm1−/−/TgEYFP-NYX mice. Fig. 5 shows that in the absence of Trpm1−/− nyctalopin staining in the DBC dendrites is indistinguishable from wildtype. Just as in wildtype retinas, EYFP-nyctalopin colocalizes with mGluR6 and is closely associated with ribeye, which is present as part of the photoreceptor ribbon synapse (Schmitz et al., 2000). Further, these data show that mGluR6 also is localized and expressed in a normal pattern in the Trpm1−/− mice (Koike et al., 2010). In summary, the data presented support the hypothesis that nyctalopin is required for the localization of TRPM1 at the tips of DBC dendrites in the mouse retina.

Fig. 5
Trpm1−/− mice have normal expression of nyctalopin at the dendrites of the DBCs

Nyctalopin interacts with TRPM1

Given the dependence of TRPM1 expression on the tips of DBC dendrites on nyctalopin we examined whether the two proteins interacted directly, using a genetic and a biochemical approach.

A membrane yeast two hybrid (MYTH) approach was used to determine if nyctalopin and TRPM1 interacted (Johnsson and Varshavsky, 1994; Stagljar et al., 1998). This system utilizes a split-ubiquitin as the interaction sensor (Fig. 6A). Bait proteins are fused to the C-terminus of ubiquitin that is, in turn, fused to a LexA-VP16 transcription factor. Prey proteins are fused to the N-terminus of ubiquitin containing an I13G mutation, which prevents the two ubiquitin fragments from interacting. Only if bait and prey proteins interact is a functional ubiquitin formed, allowing recruitment of cellular ubiquitinases, which cleave the fusion bait protein freeing LexA-VP16 to enter the nucleus and activate reporter genes (Fig. 6A).

Fig. 6
TRPM1 interacts with Nyctalopin

As a positive control we tested interaction between mGluR6 and GαO (Tian and Kammermeier, 2006). We also tested interaction between mGluR6 and TRPM1, nyctalopin and TRPM1, and nyctalopin and Fur4 (Fig. 6B). The incorporation of both bait and prey plasmids was confirmed by growth on double dropout plates (Fig. 6B, column 1). Interactions were tested by growth on quadruple dropout plates (Fig. 6B, column 2) and by a β-galactosidase assay (Fig. 6B, column 3). As expected, mGluR6 and GαO show interaction by growth on quadruple dropout plates and a positive β-galactosidase assay (Fig. 6B, row 1). There was no indication that mGluR6 and TRPM1 interacted in this system (Fig. 6B, row 2). In contrast, nyctalopin and TRPM1 showed interactions based on both growth and the β-galactosidase assay (Fig. 6B, row 3). To ensure that this was not a non-specific interaction we tested nyctalopin with Fur4, a yeast plasma membrane protein, and there was no indication of growth on the quadruple dropout plates (Fig. 6B, row 4). Combined, these data indicate that in the MYTH system nyctalopin and TRPM1 interact.

To further validate this interaction was real we used immunoprecipitation assays. Nyctalopin was tagged with YFP and a Strep tag. The Strep tag was used to pull down complexes with Strep-tactin magnetic beads (Schmidt and Skerra, 2007). A FLAG tag and GFP was added to TRPM1 (FG-TRPM1). Both tagged constructs were cloned into pcDNA3.1 and co-transfected either alone or in combination into HEK293T cells. Samples from whole cell lysates (L) and proteins bound and eluted from the Strep-tactin beads (E) were analyzed by western blot (Fig. 6). The presence of SY-NYX and FG-TRPM1 was detected by antibodies to GFP and FLAG, respectively. In the mock transfected cells there were no bands when using either anti-GFP or FLAG indicating the antibodies were specific (Fig. 6C, lane 1 & 2). Expression of SY-NYX alone resulted in the expression of the predicted 90 kDa SY-NYX protein (Fig. 6C, lane 3), which was greatly enriched after pull-down with strep-tactin beads, as seen by the increase in band intensity (Fig. 6B, lane 4). When FG-TRPM1 was transfected alone a band corresponding to the 200 kDa FG-TRPM1 was present in the lysate (Fig. 6B, lane 5) and FG-TRPM1 did not bind to the strep-tactin beads (Fig. 6B, lane 6). When SY-NYX and FG-TRPM1 were co-transfected, both fusion proteins were present in the lysate (Fig. 6B, lane 7). Following strep-tactin purification of SY-NYX a band for FG-TRPM1 was present in the eluted fraction (Fig. 6B, lane 8) indicating the two proteins interact. In the eluted fraction, the SY-NYX band is more intense than FG-TRPM1suggesting that only a fraction of the two proteins are interacting. This situation also is consistent with what we see in the retina, namely nyctalopin is only required for the expression of TRPM1 at the tips of the DBCs and not in the intracellular compartments. Combined with yeast two hybrid data, these results demonstrate that nyctalopin and TRPM1 bind to one another.


The TRPM1 channel is the non-selective cation channel mediating the retinal DBC light-response (Koike et al., 2010). How TRPM1 is localized to the DBC cell membrane in close proximity to other members of the signal transduction cascade, such as mGluR6, is an important question. In this study, we establish that the interaction between TRPM1 and nyctalopin is essential for TRPM1 localization to the tips of the DBC dendrites. Interestingly, nyctalopin is an extracellular protein that is attached to the membrane either by a GPI anchor in humans, or a single transmembrane domain in mice (O'Connor et al., 2005). Human nyctalopin has no intracellular region and mouse nyctalopin contains only three intracellular amino acids, which suggests that an entirely extracellular protein anchored to the membrane is required for TRPM1 subcellular localization. The localization of membrane proteins to the synapses is generally thought to involve cytoskeletal scaffolding proteins, many of which contain PDZ domains (Feng and Zhang, 2009). Given that nyctalopin is extracellular; another ancillary transmembrane protein would be needed to interact with intracellular scaffolding complexes to hold the TRPM1 channel in the DBC synapse. Currently, no such protein has been identified in the DBC dendrite. In addition to mGluR6 and TRPM1, a number of secondary proteins including Gβ5, R9AP, RGS7, RGS11, and nyctalopin have been localized to the DBC dendritic tip (Cao et al., 2009; Gregg et al., 2007; Morgans et al., 2007; Rao et al., 2007). Nyctalopin is the only extracellular protein in this group, and therefore unlikely to be a direct component of the transduction cascade, suggesting it is acting as an accessory protein to regulate localization of the TRPM1 channel to the synapse. Exactly how nyctalopin positions the TRPM1 channel to the region of the postsynaptic density (PSD) is unknown.

The role of accessory targeting proteins has been studied most extensively for ionotropic AMPA and NMDA channels (see review Diaz, 2010). For example, transmembrane AMPA receptor regulatory proteins (TARPs) are accessory proteins that mainly alter channel properties though some also promote surface expression and targeting and/or stability of AMPA receptors. This targeting function appears restricted to TARPs that contain intracellular domains that interact with PDZ domains of proteins that are part of the PSD. The PSD serves as a scaffold for synaptic proteins to bind the PDZ domains or proteins already bound to the scaffold. This provides for a dynamic association of channels as well as a large number of signaling molecules localized to the synapse. Many channels bind directly to PDZ domains, and this has been shown to be the case for mGluR6 (Hirbec et al., 2002). This interaction may be the reason mGluR6 is correctly localized in the absence of nyctalopin (Ball et al., 2003). TRPM1 has not been shown to bind to PDZ domains and our data argues that its localization to the PSD is dependent on nyctalopin.

Nyctalopin’s main extracellular domain is a curved structure, formed by the LRRs, which is a known interaction domain (Bella et al., 2008). This structure is critical to nyctalopin’s function because mutations that are predicted to disrupt it cause CSNB1 (Matsushima et al., 2005). Nyctalopin also contains a number of potential glycosylation sites, which could be functionally important. The exact nyctalopin motif mediating the TRPM1 interaction is currently not known. Several LRR containing proteins, densin-180, Erbin, LGI, NGL and SALM families of proteins and LRRTM2, have been shown to be important in synapse function (de Wit et al., 2009; Ko and Kim, 2007), although none are exclusively extracellular and required for targeting of binding partners to the synapse.

One model for TRPM1 targeting to the DBC dendrites by nyctalopin would be that positioning only occurs in the dendritic tips after trans-Golgi trafficking of TRPM1 and insertion into the membrane. Nyctalopin expression is restricted to the DBC dendritic tips (Gregg et al., 2007) and its leucine-rich repeat domain is postulated to interact with integrins or other cell matrix proteins (Heinegard, 2009). Therefore, a plausible model is that nyctalopin is localized to the DBC dendritic tips, after which TRPM1 interacts, thereby establishing its location. This would argue separate trafficking mechanisms for TRPM1 and nyctalopin, which is consistent with our results that the dendritic targeting of nyctalopin is not dependent on interactions with TRPM1, since nyctalopin is localized correctly in TRPM1 null mice (Fig. 5). This post-processing model of interaction also is consistent with our heterologous expression data, where only a small fraction of TRPM1 is co-precipitated with nyctalopin (Fig. 6).

In conclusion, this is the first report showing an auxiliary extracellular protein is required for localizing a TRP channel to a specific neuronal compartment or synapse. While the expression of nyctalopin and TRPM1 is somewhat restricted in tissue distribution, there are a large number of leucine-rich repeat proteins and TRP family members that could have a similar relationship. Elucidating the detailed mechanism of TRPM1 dependence on nyctalopin will lead to new findings with respect to the mechanisms controlling the targeting of TRP channels to specific neuronal compartments.


We thank Dr. Vadim Arshavsky for many valuable discussions. This work was supported by funding from the NEI EY12354 (RGG) and National Natural Science Foundation of China 81000395 (YS).


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