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Infect Immun. 1989 May; 57(5): 1517–1523.
PMCID: PMC313308

Nucleotide sequence of the plasminogen activator gene of Yersinia pestis: relationship to ompT of Escherichia coli and gene E of Salmonella typhimurium.


We have determined the nucleotide sequence of the 1.4-kilobase DNA fragment containing the plasminogen activator gene (pla) of Yersinia pestis, which determines both plasminogen activator and coagulase activities of the species. The sequence revealed the presence of a 936-base-pair open reading frame that constitutes the pla gene. This reading frame encodes a 312-amino-acid protein of 34.6 kilodaltons and containing a putative 20-amino-acid signal sequence. The presence of a single large open reading frame is consistent with our previous conclusion that the two Pla proteins which appear in the outer membrane of pla+ Y. pestis are derived from a common precursor. The deduced amino acid sequence of Pla revealed that it possesses a high degree of homology to the products of gene E of Salmonella typhimurium and ompT of Escherichia coli but does not possess significant homology to other plasminogen activators of known sequence. We also identified a transcription unit that resides on the complimentary strand and overlaps the pla gene.

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  • Sugimura K, Nishihara T. Purification, characterization, and primary structure of Escherichia coli protease VII with specificity for paired basic residues: identity of protease VII and OmpT. J Bacteriol. 1988 Dec;170(12):5625–5632. [PMC free article] [PubMed]
  • Aiba H, Matsuyama S, Mizuno T, Mizushima S. Function of micF as an antisense RNA in osmoregulatory expression of the ompF gene in Escherichia coli. J Bacteriol. 1987 Jul;169(7):3007–3012. [PMC free article] [PubMed]
  • Beesley ED, Brubaker RR, Janssen WA, Surgalla MJ. Pesticins. 3. Expression of coagulase and mechanism of fibrinolysis. J Bacteriol. 1967 Jul;94(1):19–26. [PMC free article] [PubMed]
  • Ben-Gurion R, Shafferman A. Essential virulence determinants of different Yersinia species are carried on a common plasmid. Plasmid. 1981 Mar;5(2):183–187. [PubMed]
  • Brubaker RR, Beesley ED, Surgalla MJ. Pasteurella pestis: Role of Pesticin I and Iron in Experimental Plague. Science. 1965 Jul 23;149(3682):422–424. [PubMed]
  • Chen EY, Seeburg PH. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA. DNA. 1985 Apr;4(2):165–170. [PubMed]
  • Ferber DM, Brubaker RR. Plasmids in Yersinia pestis. Infect Immun. 1981 Feb;31(2):839–841. [PMC free article] [PubMed]
  • Fields PI, Swanson RV, Haidaris CG, Heffron F. Mutants of Salmonella typhimurium that cannot survive within the macrophage are avirulent. Proc Natl Acad Sci U S A. 1986 Jul;83(14):5189–5193. [PubMed]
  • Finegold MJ. Pathogenesis of plague. A review of plague deaths in the United States during the last decade. Am J Med. 1968 Oct;45(4):549–554. [PubMed]
  • Fiss EH, Hollifield WC, Jr, Neilands JB. Absence of ferric enterobactin receptor modification activity in mutants of Escherichia coli K-12 lacking protein a. Biochem Biophys Res Commun. 1979 Nov 14;91(1):29–34. [PubMed]
  • Gayda RC, Henderson GW, Markovitz A. Neuroactive drugs inhibit trypsin and outer membrane protein processing in Escherichia coli K-12. Proc Natl Acad Sci U S A. 1979 May;76(5):2138–2142. [PubMed]
  • Gold L, Pribnow D, Schneider T, Shinedling S, Singer BS, Stormo G. Translational initiation in prokaryotes. Annu Rev Microbiol. 1981;35:365–403. [PubMed]
  • Grodberg J, Dunn JJ. ompT encodes the Escherichia coli outer membrane protease that cleaves T7 RNA polymerase during purification. J Bacteriol. 1988 Mar;170(3):1245–1253. [PMC free article] [PubMed]
  • Grodberg J, Lundrigan MD, Toledo DL, Mangel WF, Dunn JJ. Complete nucleotide sequence and deduced amino acid sequence of the ompT gene of Escherichia coli K-12. Nucleic Acids Res. 1988 Feb 11;16(3):1209–1209. [PMC free article] [PubMed]
  • Henikoff S. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene. 1984 Jun;28(3):351–359. [PubMed]
  • Hopp TP, Woods KR. Prediction of protein antigenic determinants from amino acid sequences. Proc Natl Acad Sci U S A. 1981 Jun;78(6):3824–3828. [PubMed]
  • Jonsson P, Lindberg M, Haraldsson I, Wadström T. Virulence of Staphylococcus aureus in a mouse mastitis model: studies of alpha hemolysin, coagulase, and protein A as possible virulence determinants with protoplast fusion and gene cloning. Infect Immun. 1985 Sep;49(3):765–769. [PMC free article] [PubMed]
  • Leytus SP, Bowles LK, Konisky J, Mangel WF. Activation of plasminogen to plasmin by a protease associated with the outer membrane of Escherichia coli. Proc Natl Acad Sci U S A. 1981 Mar;78(3):1485–1489. [PubMed]
  • Lipman DJ, Pearson WR. Rapid and sensitive protein similarity searches. Science. 1985 Mar 22;227(4693):1435–1441. [PubMed]
  • Oliver D. Protein secretion in Escherichia coli. Annu Rev Microbiol. 1985;39:615–648. [PubMed]
  • Rosenberg M, Court D. Regulatory sequences involved in the promotion and termination of RNA transcription. Annu Rev Genet. 1979;13:319–353. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PubMed]
  • Shine J, Dalgarno L. The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342–1346. [PubMed]
  • Simons RW, Houman F, Kleckner N. Improved single and multicopy lac-based cloning vectors for protein and operon fusions. Gene. 1987;53(1):85–96. [PubMed]
  • Sodeinde OA, Goguen JD. Genetic analysis of the 9.5-kilobase virulence plasmid of Yersinia pestis. Infect Immun. 1988 Oct;56(10):2743–2748. [PMC free article] [PubMed]
  • Sodeinde OA, Sample AK, Brubaker RR, Goguen JD. Plasminogen activator/coagulase gene of Yersinia pestis is responsible for degradation of plasmid-encoded outer membrane proteins. Infect Immun. 1988 Oct;56(10):2749–2752. [PMC free article] [PubMed]
  • Straley SC, Bowmer WS. Virulence genes regulated at the transcriptional level by Ca2+ in Yersinia pestis include structural genes for outer membrane proteins. Infect Immun. 1986 Feb;51(2):445–454. [PMC free article] [PubMed]
  • Straley SC, Brubaker RR. Localization in Yersinia pestis of peptides associated with virulence. Infect Immun. 1982 Apr;36(1):129–135. [PMC free article] [PubMed]
  • Straley SC, Harmon PA. Growth in mouse peritoneal macrophages of Yersinia pestis lacking established virulence determinants. Infect Immun. 1984 Sep;45(3):649–654. [PMC free article] [PubMed]
  • Tilghman SM, Tiemeier DC, Polsky F, Edgell MH, Seidman JG, Leder A, Enquist LW, Norman B, Leder P. Cloning specific segments of the mammalian genome: bacteriophage lambda containing mouse globin and surrounding gene sequences. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4406–4410. [PubMed]
  • Yanisch-Perron C, Vieira J, Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. [PubMed]
  • Yu GQ, Hong JS. Identification and nucleotide sequence of the activator gene of the externally induced phosphoglycerate transport system of Salmonella typhimurium. Gene. 1986;45(1):51–57. [PubMed]

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