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Infect Immun. 1989 May; 57(5): 1427–1431.
PMCID: PMC313294

Purification and partial characterization of a type-specific antigen of Rickettsia tsutsugamushi.


A type-specific antigen (54- to 56-kilodalton polypeptide) in the envelope of Rickettsia tsutsugamushi was purified from each of three prototype strains (Gilliam, Karp, and Kato) by a combination of mild anionic detergent treatment, gel filtration, and reverse-phase high-performance liquid chromatography. The purified antigens from the three strains were shown to have similar amino acid compositions: primarily aspartic acid, glutamic acid, and glycine, with lesser amounts of cysteine, methionine, and tyrosine. The N-terminal amino acid sequences of the antigens were 74.3% homologous among the three strains.

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Selected References

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  • Amano K, Tamura A, Ohashi N, Urakami H, Kaya S, Fukushi K. Deficiency of peptidoglycan and lipopolysaccharide components in Rickettsia tsutsugamushi. Infect Immun. 1987 Sep;55(9):2290–2292. [PMC free article] [PubMed]
  • Barbour AG, Amano K, Hackstadt T, Perry L, Caldwell HD. Chlamydia trachomatis has penicillin-binding proteins but not detectable muramic acid. J Bacteriol. 1982 Jul;151(1):420–428. [PMC free article] [PubMed]
  • Bavoil P, Ohlin A, Schachter J. Role of disulfide bonding in outer membrane structure and permeability in Chlamydia trachomatis. Infect Immun. 1984 May;44(2):479–485. [PMC free article] [PubMed]
  • Chen R, Krämer C, Schmidmayr W, Henning U. Primary structure of major outer membrane protein I of Escherichia coli B/r. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5014–5017. [PubMed]
  • Heckels JE. Structural comparison of Neisseria gonorrhoeae outer membrane proteins. J Bacteriol. 1981 Feb;145(2):736–742. [PMC free article] [PubMed]
  • Klapper DG, Wilde CE, 3rd, Capra JD. Automated amino acid sequence of small peptides utilizing Polybrene. Anal Biochem. 1978 Mar;85(1):126–131. [PubMed]
  • LOWRY OH, ROSEBROUGH NJ, FARR AL, RANDALL RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed]
  • Merril CR, Goldman D, Sedman SA, Ebert MH. Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science. 1981 Mar 27;211(4489):1437–1438. [PubMed]
  • Murata M, Yoshida Y, Osono M, Ohashi N, Oyanagi M, Urakami H, Tamura A, Nogami S, Tanaka H, Kawamura A., Jr Production and characterization of monoclonal strain-specific antibodies against prototype strains of Rickettsia tsutsugamushi. Microbiol Immunol. 1986;30(7):599–610. [PubMed]
  • Newhall WJ, Jones RB. Disulfide-linked oligomers of the major outer membrane protein of chlamydiae. J Bacteriol. 1983 May;154(2):998–1001. [PMC free article] [PubMed]
  • Tamura A. [Pathogenic agents of Rickettsia tsutsugamushi]. Nihon Saikingaku Zasshi. 1988 May;43(3):629–639. [PubMed]
  • Tamura A, Ohashi N, Urakami H, Takahashi K, Oyanagi M. Analysis of polypeptide composition and antigenic components of Rickettsia tsutsugamushi by polyacrylamide gel electrophoresis and immunoblotting. Infect Immun. 1985 Jun;48(3):671–675. [PMC free article] [PubMed]
  • Tamura A, Takahashi K, Tsuruhara T, Urakami H, Miyamura S, Sekikawa H, Kenmotsu M, Shibata M, Abe S, Nezu H. Isolation of Rickettsia tsutsugamushi antigenically different from Kato, Karp, and Gilliam strains from patients. Microbiol Immunol. 1984;28(8):873–882. [PubMed]
  • Tamura A, Urakami H, Tsuruhara T. Purification of Rickettsia tsutsugamushi by Percoll density gradient centrifugation. Microbiol Immunol. 1982;26(4):321–328. [PubMed]
  • Urakami H, Ohashi N, Tsuruhara T, Tamura A. Characterization of polypeptides in Rickettsia tsutsugamushi: effect of preparative conditions on migration of polypeptides in polyacrylamide gel electrophoresis. Infect Immun. 1986 Mar;51(3):948–952. [PMC free article] [PubMed]
  • Urakami H, Tsuruhara T, Tamura A. Penetration of Rickettsia tsutsugamushi into cultured mouse fibroblasts (L cells): an electron microscopic observation. Microbiol Immunol. 1983;27(3):251–263. [PubMed]
  • Yamamoto S, Kawabata N, Tamura A, Urakami H, Ohashi N, Murata M, Yoshida Y, Kawamura A., Jr Immunological properties of Rickettsia tsutsugamushi, Kawasaki strain, isolated from a patient in Kyushu. Microbiol Immunol. 1986;30(7):611–620. [PubMed]

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