Tumor cell repopulation stimulated by cell death in vitro
To test our hypothesis, we carried out experiments to examine if dying tumor cells could indeed stimulate the growth of living tumor cells. In order to simulate in vivo
scenarios where the vast majority of tumor cells are killed by radiation or chemotherapy, we seeded a small number (about 500) of firefly luciferase (Fluc)-labeled murine breast cancer 4T1 cells onto a bed of a much larger number (2.5×105
) of unlabeled “feeder” 4T1 tumor cells that were irradiated with x-rays at different doses. Growth of the small number of labeled living cells was then monitored through non-invasive bioluminescence imaging10
(see Supplementary Fig. 1
for data validating bioluminescence quantification of tumor cells). Our results indicated that 4T1Fluc cells grew significantly faster when seeded onto dying cells than when seeded alone (). In addition, there was a dose-dependent response from the feeder cells, with non-irradiated feeder cells exhibited no supportive roles and those irradiated with higher radiation doses exhibiting higher growth-enhancing ability (). Additional supporting evidence came from combinations of other dying vs living cell types, which also showed growth-stimulating properties (Supplementary Figs. 2
In vitro and in vivo evidence for the generation of strong growth-stimulating signals in dying cells.
Because in solid tumors stromal cells play important roles in modulating tumor growth, we also evaluated whether dying fibroblast cells could promote tumor cell growth. Lethally irradiated mouse embryonic fibroblast cells stimulated the growth of different Fluc-labeled tumor cells significantly in vitro
Tumor cell repopulation stimulated by cell death in vivo
We next examined whether cell death-stimulated tumor cell proliferation could be observed in vivo. A mix of untreated, Fluc-labeled and lethally irradiated, unlabeled tumor cells (at a ratio of 1:250, or 1000 live 4T1Fluc cells mixed with 2.5×105 unlabeled, lethally irradiated 4T1 cells) were injected subcutaneously into the hind legs of nude mice. Subsequently the growth of the Fluc-labeled tumor cells was followed non-invasively over time through bioluminescence imaging. As controls, an equal number of Fluc-labeled 4T1 tumor cells mixed with live, unlabeled 4T1 tumor cells were injected into contra-lateral hind legs. Our results show that the presence of lethally irradiated tumor cells significantly increased the growth of Fluc-labeled tumor cells when compared with Fluc-labeled tumor cells injected together with live tumor cells. In fact the difference in the intensities of luciferase signals between the two groups grew exponentially larger and reached as great as 700 fold at the end of the experiment ().
Similar in vivo tumor growth-promoting properties were also observed for mouse embryonic fibroblasts (MEF) that were irradiated (). Fluc-labeled 4T1 co-injected with irradiated fibroblast cells grew to signal intensities 400 fold more than those from 4T1-Fluc cells injected alone in contra-lateral hind legs.
Caspase 3 regulates tumor cell repopulation in vitro
What is the molecular mechanism for the observed cell death-stimulated proliferation of surviving cells? We reasoned that among the many cellular processes activated/deactivated in dying cells, factors/processes directly responsible for cell death are most likely to play key roles in regulating the growth-promoting properties of dying cells. Therefore, we hypothesized that caspases, the proteases that were involved in both the initiation and the execution of programmed cell death11
, might be involved in regulating the growth-promoting properties of dying cells. To examine this hypothesis, we obtained mouse embryonic fibroblast (MEF) cells with genetic deletion of their Caspase 3
and evaluated the ability of these cells to support the growth of a small number of Fluc-labeled tumor cells. Our results () indicate clearly that deficiencies in Casp3
significantly compromised the ability of lethally irradiated MEF cells to stimulate the growth of Fluc-labeled murine (4T1) and human (MDA-MB231 and HCT116) tumor cells. The proliferation of Fluc-labeled tumor cells among the irradiated Caspase 3
) cells was close to Fluc-labeled tumor cells seeded alone, suggesting that caspase 3 was largely responsible for growth stimulation by dying cells.
The role of caspase 3 in cell death-induced tumor cell proliferation in vitro and in vivo.
The importance of caspase 3 was also confirmed in lethally irradiated 4T1 cells through shRNA-mediated knockdown of Casp3
expression in feeder cells ()
. It was similarly confirmed in the human breast cancer cell line MCF7, which is deficient in casp3 expression. Exogenous expression of caspase 3 significantly increased the ability of lethally irradiated MCF-7 cells to promote co-seeded MCF-7Fluc cells (Supplementary Fig. 4
and wild type MEF cells showed similar clonogenic survival after irradiation (Supplementary Fig. 5
), we reasoned the observed defect for Casp3−/−
MEF cells was not due to the lack of cell death in these cells after radiation. We obtained further data indicating how caspase 3 status will affect the modes of cell death in 4T1 (Supplementary Figure 6
), MEF (Supplementary Figure 7
), and MCF-7 (Supplementary Figure 8
) after radiation. Please refer to Supplementary Notes
section for additional information on these data.
To examine whether the proteolytic activity of caspase 3 is required, we transduced a dominant-negative version of caspase 3 (C163A)14
to inhibit caspase 3 cleavage activity in 4T1 cells. Our data indicated that 4T1 cells transduced with a dominant-negative Casp3
gene completely lost its ability to support the growth of 4T1Fluc cells (). We also obtained similar results by use of a chemical inhibitor of caspase 3 z-VAD-fmk (Supplementary Fig. 9
To confirm that caspase 3 was activated in irradiated cells, we carried out comprehensive immunoblot analyses of various proteins in the apoptotic pathway in irradiated 4T1 (,
see Supplementary Table 1
for antibody information), and MEF (Supplementary Fig. 10
) cells. Our data indicate caspase 3&9 and downstream cytochrome c were activated in both 4T1 and MEF cells in a dose-dependent manner while caspase 8 was not activated.
Caspase 3 regulation of tumor cell repopulation in vivo
To examine the importance of caspase 3 in cell-death stimulated tumor repopulation in vivo
, lethally irradiated MEF cells with different capase 3 status were mixed together with a small number (500) of Fluc-labeled 4T1 cells and injected subcutaneously into nude mice (). In contrast to potent stimulation of 4T1Fluc tumor cellular growth by lethally irradiated wild type MEF cells, significantly attenuated growth stimulation was observed for lethally irradiated caspase 3-deficient MEF cells. The difference between the two groups was as great as 1000 fold at later stages of observation (). In fact, in separate experiments, 4T1-Fluc cells injected together with lethally irradiated caspase 3-deficient MEF cells grew at a similar rate as those 4T1Fluc cells injected alone in the contra-lateral legs, indicating a lack of growth stimulation from Casp3−/−
cells (Supplementary Fig. 11
The importance of caspase 3 in regulating growth-promoting properties of dying cells in vivo was also confirmed by co-injecting 4T1-Fluc cells with lethally irradiated 4T1 transduced with an shRNA minigene targeted against caspase 3 (). A significant reduction in the ability of lethally irradiated 4T1 cells to stimulate the growth of 4T1-Fluc cells were observed, consistent with the results obtained with Casp3−/− MEF cells.
Caspase 3 activation and growth of injected tumor cells in established tumors
To show that caspase 3 activation is indeed activated in solid tumors during radiotherapy, we established murine 4T1 tumor cells transduced with a novel luciferase/GFP and proteasome-based reporter (,
left panel). Supplementary Fig. 12
shows data supporting the functionality of our caspase 3 reporter. Tumors were irradiated with x-rays (6 Gy) when they reach 5-7 mm in diameter and observed for bioluminescence signals periodically afterwards. It is clear that caspase 3 was significantly activated (as much as 30 fold at its peak) at days 3, 5, and 7 after radiotherapy (,
Relationship between caspase activation and growth of externally injected tumor cells in the irradiated tumor microenvironment.
In another experiment, a small number of Fluc-labeled 4T1 tumor cells (about 1000) were injected into irradiated and non-irradiated tumors in the same group of mice and observed for growth. Our results () demonstrated that cells injected into irradiated tumors grew at a significantly faster rate than those injected into the non-treated tumors, consistent with earlier results.
In still another experiment, 4T1 cells stably transduced with green fluorescence protein (GFP) was injected into irradiated tumors and allowed to grow for 5-8 days. The mice were then sacrificed and tumors excised and examined for expression of various proteins (). Our data show a clear relationship between activated caspase 3 and the proliferation of injected, GFP-labeled tumor cells in irradiated tumor cells, as demonstrated by the complementary pattern of caspase 3 staining and injected tumor cell staining. In contrast, very little injected tumor cell growth and caspase activation was observed in non-irradiate tumors. In addition to caspase activation, irradiated tumors also showed increased, localized neovaculature.
Caspase 3 activates iPLA2 to stimulate tumor repopulation
We next attempted to identify the downstream factors of caspase 3 that were involved in generating growth-promoting factors from the dying cells. We focused on calcium independent phospholipase A2
) because it had been reported that its phosopholipase activity was activated by caspase 3 cleavage15-17
. Importantly, the activation was shown to increase production of arachidonic acid (AA)15
, whose downstream eicosanoid derivatives (i.e. prostaglandin E2
), had been implicated in stimulating tumor growth18
and stem cell proliferation19
. To evaluate the potential involvement of caspase-activated iPLA2
axis in cell death-induced tumor cell proliferation, we transduced shRNA against the iPla2
gene into 4T1 tumor cells or wild type MEF cells and examined whether these cells, when lethally irradiated, could still support Fluc-labeled tumor cell growth as much as their wild-type counterpart. Our results () indicated in both MEF and 4T1 cells, expression of shiPLA2
significantly reduced ability of these cells to stimulate Fluc-labeled 4T1 cellular proliferation. We also have western blot data to indicate that iPLA2
is indeed activated in a capase 3-dependent manner (Supplementary Fig. 13
) in both 4T1 and MEF cells. These results are consistent with multiple earlier reports. 15-17
An important role for caspase 3-activated iPLA2 in facilitating cell death stimulated tumor cell repopulation.
The importance of caspase 3-mediated activation of iPLA2
was further confirmed when a truncated version of the iPla2
), which encoded an iPla2
fragment (containing the catalytic domain) that was a predicted product of caspase 3 cleavage of iPla2
, was transduced into caspase 3-deficient MEF cells. After ΔiPla2
transduction, lethally irradiated Casp3−/−
cells had significantly increased ability to stimulate the growth of Fluc-labeled 4T1-Fluc cells both in vitro
() and in vivo
and Supplementary Fig. 14
) when compared with parental Casp3−/−
We also showed that knocking down iPla2
gene expression significantly reduced the ability of lethally irradiated MEF cells to stimulate the growth of 4T1-Fluc cells in vivo
( and Supplementary Fig. 15
). Furthermore, we show that by use of established wild type or iPla2
knockdown 4T1 tumors, growth of subsequently transplanted 4T1 cells was significantly slowed down (Supplementary Fig. 16
Caspase 3 and iPLA2 stimulate AA and PGE2 release
We next conducted experiments to confirm the role of caspase 3 in regulating the activity of the iPLA2 by examining its enzymatic activities. Because arachidonic acid is one of two main catalytic products of activated iPLA2 (the other being lysophosphatidic choline, or LPC), we measured radiation-induced release of arachidonic acid into the extracellular milieu to examine potential relationship between caspase 3 and arachidonic acid release. Our results indicate that radiation stimulated the release of arachidonic acid into the supernatant significantly in wild type MEF cells (). However, the release was significantly reduced in Casp3−/−MEF cells, indicating a crucial role for caspase 3. In addition, arachidonic acid (AA) release was also significantly reduced in 4T1 cells with effective knockdown of the Casp3 gene expression ().
Regulation of radiation-induced arachidonic acid release and PGE2 production by caspase 3
Because prostaglandin E2 (PGE2), a key regulator of tumor growth, is a downstream product of AA, we measured PGE2 production induced by ionizing radiation in the supernatants of cells. Our results () indicated that exposure to ionizing radiation significantly induced the production of PGE2 in wild type MEF cells as well as in 4T1 tumor cells. However, in Casp3−/− MEF cells and in 4T1 cells transduced with a shRNA against caspase 3, the production of PGE2 in irradiated cells was significantly reduced. In contrast, transduction of a constitutively active, truncated iPla2 (which is equivalent to a caspase-cleaved version of iPLA2), significantly restored PGE2 production in caspase 3 deficient (Casp3−/−) MEF cells.
In an additional experiments (Supplementary Fig. 17
), we showed that cyclooxygenase 2 (Cox 2) and its upstream transcription factor NF-kB, which have crucial roles in PGE2
production, were activated in a caspase 3-independent manner. Consistently, indomethacin (a Cox 1&2 inhibitor) administration effectively suppressed growth of intratumorally injected 4T1 and HCT116 cells (Supplementary Fig. 18
The importance of PGE2
was demonstrated by the fact that treatment of a small number (1000) of tumor cells with PGE2
gave the treated cells a significant head start, allowing them to grow at a faster pace than non-treated cells when injected into mice (). On the other hand, shRNA mediated down-regulation of EP2
, a receptor for PGE2
, in 4T1Fluc cells significantly attenuated the proliferation of the latter when seeded together with lethally irradiated 4T1 cells (Supplementary Fig. 19)
Caspase 3 and radiosensitivities of tumors in mice and man
To examine the intriguing and exciting prospect of enhancing cancer radiotherapy through inhibition of caspase 3, we carried out two experiments in mouse tumor models.
In the first experiment, we used MCF-7 cells, which are naturally deficient in caspase 3 expression. We established tumor xenograft in female nude mice using both parental MCF-7 cells as well as a modified MCF-7 cell line with an exogenously transduced copy of caspase 3 gene. The transduction of CASP3
into parental MCF-7 cells rendered them significantly more susceptible to apoptosis (Supplementary Fig. 8
). Surprisingly, caspase 3 transduction also enabled MCF-7 cells to form tumors at a faster pace (). Mostly importantly, the presence of caspase 3 makes MCF-7CASP3
tumors significantly more resistant to radiotherapy than parental MCF-7 tumors, which disappeared completely after 2×6 Gy or x-rays and did not re-grow during the entire course of observation (). In contrast, MCF-7CASP3
kept growing after irradiation despite being slowed down. These surprising results indicated that deficiency of caspase 3 activity in tumor cells will render the whole tumor significantly more susceptible to radiotherapy.
Deficiency in caspase 3 correlated with tumor response to therapy in mice as well as in human patients.
In the second experiment, we attempted to examine the potential importance of stroma-derived caspase 3 activities in tumor response to radiotherapy because stroma accounted for a significant percentage of tumor mass. We established tumors in wild type as well as Casp3−/− mice with B16F10 cells, which is a metastatic and radioresistant tumor line syngeneic with the C57BL/6 mice. We carried out radiotherapy (2×8 Gy) when tumor reached 5-7 mm in diameter. Our results indicate that B16F10 tumor grown in Casp3−/− mice exhibit significant sensitivity to radiotherapy when compared with those in wild type C57BL/6 mice (). Our results therefore confirmed the importance of caspase 3 in both tumor cells and tumor stroma in stimulating tumor response to radiotherapy.
In order to determine the relevance of our newly discovered pathway for caspase-mediated tumor repopulation in human cancer treatment, we evaluated caspase 3 status in two cohorts of human cancer patients. In the first cohort, 39 head and neck cancer patients treated with radiotherapy, or chemo-radiotherapy at the Princess Margaret Hospital in Toronto, Canada (see Supplementary Table 2
for details of patient characteristics) were examined for activated caspase 3 through immunohistochemical analysis. Consistent with our results in mice (), patients with high cleaved (and thus activated) caspase 3 levels in their tumor samples showed significantly (p=0.011) higher rate of tumor recurrence ( and Supplementary Fig. 20
). In the second cohort, 48 advanced stage breast cancer patients treated at Shanghai No. 1 People’s hospital (see Supplementary Table 3
for details on patient characteristics) were analyzed for activated caspase 3 expression through IHC analysis. Results indicated that patients with high cleaved caspase 3 staining were at a significant (p=0.0002) disadvantage in terms of survival ( and Supplementary Fig. 21
). A further piece of supporting evidence came from our own analysis of public domain microarray data from a previously published study20
. In 249 breast cancer patients from Sweden and Singapore, elevated caspase 3 mRNA levels correlated with significantly elevated risk (p=0.0001) of relapse (Supplementary Fig. 22
). Taken together, we conclude that elevated tumor caspase 3 levels predict for worse treatment outcomes in human cancer patients.