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Trends Cell Biol. Author manuscript; available in PMC 2012 July 1.
Published in final edited form as:
PMCID: PMC3128673
NIHMSID: NIHMS285942

Rabs and the Exocyst in Ciliogenesis, Tubulogenesis, and Beyond

Abstract

The exocyst -- an octameric protein complex mediating vesicle tethering at the plasma membrane for exocytosis -- is a downstream effector of the Rab proteins Rab8 and Rab11, which are key regulators of membrane trafficking from the trans-Golgi network and recycling endosome to the plasma membrane. Rab11 and Rab8 coordinate their actions via Rabin8, the guanine nucleotide exchange factor of Rab8. A cascade of protein-protein interactions involving the Rabs and the exocyst complex couples the generation of secretory vesicles at donor compartments to their docking and fusion at the plasma membrane. Here, we discuss recent work implicating Rab proteins and the exocyst in primary ciliogenesis and epithelial lumenogenesis. In addition, we discuss early work in the budding yeast Saccharomyces cerevisiae, which provided initial insight into the molecular mechanisms of polarized exocytosis.

Polarized exocytosis

Cells are not created equal. The diversity of cell structure and function is vital for most organisms. Despite this diversity, however, common principles are often found to operate within cells during various processes. One such common principle concerns polarized exocytosis, in which secretory vesicles carrying proteins such as receptors and ion channels are generated from the trans-Golgi network (TGN) or recycling endosomes, and transported to and eventually fused with specific areas of the plasma membrane. Several proteins play key roles in exocytosis. The exocyst, a multi-protein complex consisting of Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70 and Exo84, mediates the tethering of secretory vesicles to the plasma membrane before SNARE-mediated fusion [1,2]. The Rab family of small GTPases are master regulators in exocytosis. In their GTP-bound form, Rab proteins interact with downstream effectors, thereby controlling various steps of exocytosis [1,3]. Polarized exocytosis is important for a wide range of processes from the asymmetric growth of yeast cells to neurite branching and tumor invasion. Recently, several papers published in quick succession highlight the role of membrane trafficking in primary ciliogenesis and epithelial tube formation. We will discuss the role of Rabs and the exocyst in these processes and trace back to early work in yeast, which provided initial insights into the molecular basis of polarized exocytosis.

Primary Ciliogenesis

Most cells have a single microtubule-based membrane projection called the primary cilium. This cell surface “antenna” is enriched with important molecules such as G-protein coupled receptors (GPCRs) like Smoothened, somatostatin receptors, and rhodopsin, which are central to several signaling pathways. Defects in primary ciliogenesis have been implicated in a wide array of pathological disorders ranging from retinal degeneration to Bardet-Biedl syndrome. The generation of primary cilia involves microtubule organization and polarized membrane trafficking. A classic electron microscopy study provided sequential snapshots of the transport of secretory vesicles to the site of ciliogenesis [4]. The small GTPase Rab8 is involved in membrane trafficking from the TGN and recycling endosome to the plasma membrane [5,6]. Disruption of Rab8 in frog photoreceptor cells blocks rhodopsin transport and results in an accumulation of tubulo-vesicular structures at the base of the retinal rod outer segment, a specialized form of cilia [7,8]. Studies using cultured human retinal pigment epithelium (hRPE) cells have further demonstrated that Rab8 and its guanine nucleotide exchange factor (GEF) Rabin8 play important roles in primary ciliogenesis [9,10]. Rabin8 interacts with BBS1, a component of the BBSome, which is a multi-protein complex implicated in Bardet-Biedl syndrome and is involved in cargo transport to the primary cilia [10,11]. Rabin8 is a direct downstream effector of Rab11 [12], which mediates vesicle transport from the TGN and recycling endosomes [13,14]. The GTP-bound form of Rab11 interacts with Rabin8 and kinetically stimulates its GEF activity toward Rab8. This effect is specific, as other Rab GTPases such as Rab3 and Rab5 do not exhibit any stimulatory effect on Rabin8 activity [12]. Fluorescence microscopic studies revealed that Rab11 is localized near the base of the primary cilia, and its disruption inhibits ciliogenesis [12]. It was therefore proposed that Rab11 modulates Rab8 function by activating Rabin8. This cascade of Rab activation couples cargo transport from the TGN and recycling endosomes to vesicle docking and fusion at the plasma membrane. In another interesting study, it was shown that Rab11, Rabin8, and Rab8 are involved in the de novo generation of primary cilium [15]. Using time-lapse video microscopy, the authors observed the process of Rab8 recruitment into the ciliary membrane during primary ciliogenesis in live cells, and found that Rab8 ciliary membrane localization was preceded by the trafficking of Rabin8 to the centrosome, which in turn was dependent on Rab11.

Rab proteins perform their functions through their downstream effectors. The exocyst was shown to be a downstream effector of the exocytic Rabs; the exocyst subunit Sec15 directly interacts with the GTP-bound Rab proteins including Rab8 and Rab11 [1619]. Components of the exocyst are localized at the base of the cilia [2022]. In Madin-Darby Canine Kidney (MDCK) cells, knockdown of the exocyst component Sec10 led to shorter cilia, whereas over expression of Sec10 led to elongated cilia [21]. Together, these studies implicate a role for the exocyst in ciliogenesis [2024]. We propose that a series of protein interactions from Rab11 to the exocyst control the polarized transport and docking of vesicles carrying ciliary proteins and possibly the basal body to the plasma membrane for primary ciliogenesis (Figure 1).

Figure 1
Transport of proteins to the primary cilia

Epithelial lumenogenesis

Similar to polarized trafficking to the primary cilia, the Rabs and exocyst also mediate biogenesis of the epithelial lumen. Epithelial cells form tubes such as the kidney and liver tracts. A functional lumen can be generated de novo when the apical domains of a group of epithelial cells are generated and aligned to face a common hollow space. Lumenogenesis requires polarized exocytosis, through which proteins such as polarity complexes and ion channels are transported from recycling endosomes or TGN to the apical domain of the plasma membrane [25]. In a recent paper [26], the Rab11-Rabin8-Rab8 cascade was shown to be important for proper lumenogenesis. Depletion of any of these proteins led to the formation of multiple rudimentary lumens. Inactive Rab11a, an isoform of Rab11, prevents the vesicular recruitment of Rabin8 or Rab8 and disrupts unilumenogenesis. The authors further demonstrated that the Rab GTPases regulate lumenogenesis through the exocyst complex. Knockdown of the exocyst components Sec15 or Sec10 led to inhibition of single lumen formation. Expression of a Sec15 mutant that is defective in binding Rab11 disrupts unilumenogenesis. The authors proposed a model in which the exocyst transports apical cargos, such as podocalyxin, via Rab11a-positive endosomes to create the polarized site for exocytosis (“AMIS”-apical membrane initiation site) (Figure 2). This study provides important insights into the early stages of lumenogenesis, which is pivotal for organogenesis. Thus, Rab proteins and the exocyst regulate the asymmetric distribution of proteins in epithelial cells to generate polarized tissue architecture.

Figure 2
Rab proteins and exocyst mediate membrane trafficking during the early stages of epithelial lumenogenesis

Conserved themes in Rab and exocyst function

While primary ciliogenesis and lumenogenesis are distinct cellular processes, both require the functions of the Rab11-Rabin8-Rab8 cascade and the exocyst. Cell biological discoveries of fundamental importance often came from studies in simpler model systems. Both Rabs and exocyst were first identified in the budding yeast Saccharomyces cerevisiae [2729]. Genetic and biochemical studies first revealed that the exocyst component Sec15p is a direct downstream effector of Sec4p, the Rab protein that regulates post-Golgi trafficking in yeast [16]. Also, the yeast work first led to the Rab cascade model: Sec4p is activated by its guanine nucleotide exchange factor Sec2p, which in turn is controlled by GTP-bound Ypt31p and Ypt32p, the Rab proteins that regulate vesicle budding from the TGN [30,31] (Figure 3). The mammalian homologues of Sec4p, Sec2p, and Ypt31/32p are Rab8, Rabin8, and Rab11, respectively. In yeast, Ypt32p was proposed to recruit Sec2p to the secretory vesicles. In mammalian cells, Rab11 is not only required for Rabin8 localization, but also kinetically stimulates the GEF activity of Rabin8 towards Rab8 [12,15,26]. The recruitment model and the kinetic activation model are not mutually exclusive. Future studies may reveal that both mechanisms operate in the same cells for optimal outputs. The Rab cascade is not only conserved in exocytic trafficking, but may also exist in other stages of membrane trafficking such as the early to late endosome conversion [32,33]. Altogether, these studies suggest that a highly choreographed series of Rab activation coordinates various stages of transport and may confer directionality to membrane trafficking.

Figure 3
The Rab cascade model

Location, location, location

Membrane trafficking is intimately linked to cell polarity and unilumenogenesis concerns both exocytosis and cell polarity. The intimate connection between exocytosis machinery and polarity regulators is well demonstrated in the context of epithelial lumenogenesis [26]. It was shown that the Par3/aPKC complex and the exocyst complex have a mutual dependence for their localization to the AMIS. In addition, Cdc42, a major regulator of polarity, associates with Rab8/Rab11a-positive vesicles and may act downstream of Rab8. Knockdown of Rab8 led to decreased activation of Cdc42 in cells. This effect is probably through Tuba, a GEF for Cdc42 [26]. Similarly, the polarity factors also operate in the primary cilium. As the lonely projection at the cell surface, it is intriguing how this remarkable membrane asymmetry is achieved. It was previously shown that Crumbs3 and Par3 are involved in primary ciliogenesis [34,35]. Also, it was found that the exocyst co-immunoprecipitated with Par3 in cells; knockdown of exocyst components affects ciliary localization of Par3 [21]. It is very likely that the molecular network that drives lumenogenesis described above functions during primary ciliogenesis. In yeast, the exocyst component Sec3 is a direct downstream effector of Cdc42, which spatially and kinetically regulates exocyst function during asymmetric daughter cell growth (“budding”) [36,37]. Cdc42 polarization, in turn, is in part controlled by membrane trafficking [3840]. As such, a positive feedback loop functions for the establishment and maintenance of yeast cell polarity. It will be interesting to see whether similar positive feedback loops exist in more complex processes such as ciliogenesis and lumenogenesis in higher eukaryotes.

Future perspectives

While the Rab cascade and the exocyst are critical components in the exocytic pathway, they are clearly not the only players. Recently, it was shown that Arf4, Rab11, FIP3, and the Arf GTPase-activating protein ASAP1 are important for the transport of rhodopsins to the retina outer segments [41]. The Arf4-based protein complex is probably involved in the selection and packaging of specific cargos, including GPCRs, for their delivery to the cilia. It will be interesting to know how vesicle packaging and budding are coupled to subsequent transport and docking at the plasma membrane. Once incorporated into the plasma membrane, ciliary cargos are collected by the BBSome, which acts as a planar coat that transports proteins to the cilia [11]. It will be important to determine how vesicle fusion is connected to subsequent cargo entry to the cilia, and how the BBSome is coupled to intraflagellar transport particles (IFTs) for cargo movement within the cilia. The field also awaits a better understanding of the connections between the secretory machinery and cell polarity regulators. While functional analyses [26,34,35] and immunoprecipitation experiments [21] have demonstrated a connection between Rabs and exocyst with Cdc42 and Par proteins, future experiments are called for to elucidate the molecular interactions among these proteins.

The regulation of membrane trafficking by Rab GTPases and the exocyst has profound implications in human physiology and diseases. For example, both the Rab proteins and the exocyst are implicated in tumorigenesis and cancer dissemination [4248]. Also, bacterial pathogens can hijack Rab and exocyst for their invasion and proliferation [49,50]. Elucidation of the molecular mechanism of polarized exocytosis will help us better understand many complex cell biological processes, which should ultimately result in improved treatments of human diseases.

Acknowledgments

We are grateful to Dr. Peter Novick (University of California, San Diego) for insightful discussions and suggestions. We also thank people in Wei Guo’s laboratory including Drs. Shanshan Feng, Kelly Orlando, and John Schmidt for their helpful comments. The work in Wei Guo’s laboratory has been supported by the National Institutes of Health, Pew Scholars Program in Biomedical Sciences, and American Heart Association. Amlan Das is supported by the National Kidney Foundation post-doctoral fellowship.

Footnotes

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