PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

Logo of narLink to Publisher's site
 

Figure 6

An external file that holds a picture, illustration, etc.
Object name is gke26306.jpg

HDAC-1 associates with XGrg-4-QGP, but not with mGrg-5. 293T cells were transfected with either Myc-tagged XGrg-4 QGP or Myc-tagged Grg-5 alone (lanes 1 and 2), or with a Flag-tagged HDAC-1 (lanes 3 and 4). Cell lysates were prepared 24 h after transfection. (A) Extracts from transfected cells were immunoprecipitated (IP) with an anti-Myc antibody against Myc-XGrg-4-QGP and Myc-Grg-5, and the presence of Flag-HDAC-1 in the immunoprecipitate was assayed on immunoblot (IB) with anti-Flag antibody. In lanes 1 and 2, only Myc-tagged XGrg-4-QGP or Myc-Grg-5 were transfected. In lanes 3 and 4, Myc-tagged XGrg-4-QGP or Myc-Grg-5 were co-transfected with Flag-tagged HDAC-1. Lane 5 shows the presence of HDAC-1 in the co-transfection of Myc-Grg-5 and Flag-HDAC-1. Lanes 6 and 7 show an immunoblot with anti-Myc antibody, indicating the expression of the Myc-Grg-4-QGP and Myc-Grg-5 when co-transfected with Flag-HDAC-1. (B) Extracts from transfected cells were immunoprecipitated (IP) with an anti-Flag antibody against Flag-HDAC-1. The presence of Myc-XGrg-4-QGP and Myc-Grg-5 was assayed on immunoblot (IB) with anti-Myc antibody. In lanes 1 and 2 only Myc-tagged XGrg-4-QGP or Myc-Grg-5 were transfected. In lanes 3 and 4, Myc-tagged XGrg-4-QGP or Myc-Grg-5 were co-transfected with Flag-tagged HDAC-1. Lanes 5 and 6 show an immunoblot with anti-Myc antibody, indicating the expression of Myc-Grg-4-QGP and Myc-Grg-5 when co-transfected with Flag-HDAC-1. Bands corresponding to HDAC, Grg-5 and XGrg-4 QGP are indicated with arrows. Bands labelled ‘Ig’ indicate the heavy and light chains of the antibodies used for the immune precipitation.

Images in this article

  • Figure 1
  • Figure 2
  • Figure 3
  • Figure 4
  • Figure 5
  • Figure 6
  • Figure 7
Click on the image to see a larger version.