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Nucleic Acids Res. 1986 November 25; 14(22): 8805–8817.
PMCID: PMC311912

Gene organization of the small subunit of human calcium-activated neutral protease.


The gene for the small subunit of human calcium-activated neutral protease was isolated and sequenced. It is 11 kb long and comprises 11 exons. No TATA or CAT box was found upstream of the possible transcription initiation sites, but there are three so-called G-C box sequences and one G-C box-like sequence, which are usually found in "house-keeping" genes. The first exon (exon 1) contains only the 5'-noncoding sequence and exon 2 encodes the Gly-rich hydrophobic domain. Each of the four calcium-binding loop regions is encoded by one exon (exons 7-10). The intron breakpoints in the C-terminal calcium-binding domain (exons 4-11) completely coincide with those of the chicken large subunit gene. These findings suggest that the small and large subunits have evolved from the same ancestral calcium-binding protein and have retained the original gene organization.

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Selected References

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  • Ohno S, Emori Y, Imajoh S, Kawasaki H, Kisaragi M, Suzuki K. Evolutionary origin of a calcium-dependent protease by fusion of genes for a thiol protease and a calcium-binding protein? Nature. 1984 Dec 6;312(5994):566–570. [PubMed]
  • Imajoh S, Kawasaki H, Suzuki K. The amino-terminal hydrophobic region of the small subunit of calcium-activated neutral protease (CANP) is essential for its activation by phosphatidylinositol. J Biochem. 1986 Apr;99(4):1281–1284. [PubMed]
  • Sakihama T, Kakidani H, Zenita K, Yumoto N, Kikuchi T, Sasaki T, Kannagi R, Nakanishi S, Ohmori M, Takio K, et al. A putative Ca2+-binding protein: structure of the light subunit of porcine calpain elucidated by molecular cloning and protein sequence analysis. Proc Natl Acad Sci U S A. 1985 Sep;82(18):6075–6079. [PubMed]
  • Emori Y, Kawasaki H, Imajoh S, Kawashima S, Suzuki K. Isolation and sequence analysis of cDNA clones for the small subunit of rabbit calcium-dependent protease. J Biol Chem. 1986 Jul 15;261(20):9472–9476. [PubMed]
  • Kretsinger RH, Nockolds CE. Carp muscle calcium-binding protein. II. Structure determination and general description. J Biol Chem. 1973 May 10;248(9):3313–3326. [PubMed]
  • Goodman M, Pechère JF. The evolution of muscular parvalbumins investigated by the maximum parsimony method. J Mol Evol. 1977 Apr 29;9(2):131–158. [PubMed]
  • Emori Y, Ohno S, Tobita M, Suzuki K. Gene structure of calcium-dependent protease retains the ancestral organization of the calcium-binding protein gene. FEBS Lett. 1986 Jan 6;194(2):249–252. [PubMed]
  • Simmen RC, Tanaka T, Ts'ui KF, Putkey JA, Scott MJ, Lai EC, Means AR. The structural organization of the chicken calmodulin gene. J Biol Chem. 1985 Jan 25;260(2):907–912. [PubMed]
  • Maniatis T, Hardison RC, Lacy E, Lauer J, O'Connell C, Quon D, Sim GK, Efstratiadis A. The isolation of structural genes from libraries of eucaryotic DNA. Cell. 1978 Oct;15(2):687–701. [PubMed]
  • Vieira J, Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. [PubMed]
  • Yanisch-Perron C, Vieira J, Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. [PubMed]
  • Sanger F, Nicklen S, Coulson AR. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. [PubMed]
  • Maxam AM, Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. [PubMed]
  • Southern EM. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. [PubMed]
  • Mount SM. A catalogue of splice junction sequences. Nucleic Acids Res. 1982 Jan 22;10(2):459–472. [PMC free article] [PubMed]
  • Ohno S, Emori Y, Suzuki K. Nucleotide sequence of a cDNA coding for the small subunit of human calcium-dependent protease. Nucleic Acids Res. 1986 Jul 11;14(13):5559–5559. [PMC free article] [PubMed]
  • Breathnach R, Chambon P. Organization and expression of eucaryotic split genes coding for proteins. Annu Rev Biochem. 1981;50:349–383. [PubMed]
  • Valerio D, Duyvesteyn MG, Dekker BM, Weeda G, Berkvens TM, van der Voorn L, van Ormondt H, van der Eb AJ. Adenosine deaminase: characterization and expression of a gene with a remarkable promoter. EMBO J. 1985 Feb;4(2):437–443. [PubMed]
  • Melton DW, Konecki DS, Brennand J, Caskey CT. Structure, expression, and mutation of the hypoxanthine phosphoribosyltransferase gene. Proc Natl Acad Sci U S A. 1984 Apr;81(7):2147–2151. [PubMed]
  • Melton DW, McEwan C, McKie AB, Reid AM. Expression of the mouse HPRT gene: deletional analysis of the promoter region of an X-chromosome linked housekeeping gene. Cell. 1986 Jan 31;44(2):319–328. [PubMed]
  • Gilbert W. Why genes in pieces? Nature. 1978 Feb 9;271(5645):501–501. [PubMed]
  • Stone EM, Rothblum KN, Schwartz RJ. Intron-dependent evolution of chicken glyceraldehyde phosphate dehydrogenase gene. Nature. 1985 Feb 7;313(6002):498–500. [PubMed]
  • Rieger M, Jorcano JL, Franke WW. Complete sequence of a bovine type I cytokeratin gene: conserved and variable intron positions in genes of polypeptides of the same cytokeratin subfamily. EMBO J. 1985 Sep;4(9):2261–2267. [PubMed]
  • Tyner AL, Eichman MJ, Fuchs E. The sequence of a type II keratin gene expressed in human skin: conservation of structure among all intermediate filament genes. Proc Natl Acad Sci U S A. 1985 Jul;82(14):4683–4687. [PubMed]
  • Plutzky J, Hoskins JA, Long GL, Crabtree GR. Evolution and organization of the human protein C gene. Proc Natl Acad Sci U S A. 1986 Feb;83(3):546–550. [PubMed]
  • Emori Y, Kawasaki H, Sugihara H, Imajoh S, Kawashima S, Suzuki K. Isolation and sequence analyses of cDNA clones for the large subunits of two isozymes of rabbit calcium-dependent protease. J Biol Chem. 1986 Jul 15;261(20):9465–9471. [PubMed]

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