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Nucleic Acids Res. 1986 June 11; 14(11): 4683–4690.
PMCID: PMC311474

A new method for predicting signal sequence cleavage sites.


A new method for identifying secretory signal sequences and for predicting the site of cleavage between a signal sequence and the mature exported protein is described. The predictive accuracy is estimated to be around 75-80% for both prokaryotic and eukaryotic proteins.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • von Heijne G. Signal sequences. The limits of variation. J Mol Biol. 1985 Jul 5;184(1):99–105. [PubMed]
  • von Heijne G. Patterns of amino acids near signal-sequence cleavage sites. Eur J Biochem. 1983 Jun 1;133(1):17–21. [PubMed]
  • Perlman D, Halvorson HO. A putative signal peptidase recognition site and sequence in eukaryotic and prokaryotic signal peptides. J Mol Biol. 1983 Jun 25;167(2):391–409. [PubMed]
  • Staden R. Computer methods to locate signals in nucleic acid sequences. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):505–519. [PMC free article] [PubMed]
  • Kuhn A, Wickner W. Conserved residues of the leader peptide are essential for cleavage by leader peptidase. J Biol Chem. 1985 Dec 15;260(29):15914–15918. [PubMed]
  • von Heijne G. How signal sequences maintain cleavage specificity. J Mol Biol. 1984 Feb 25;173(2):243–251. [PubMed]
  • Flinta C, Persson B, Jörnvall H, von Heijne G. Sequence determinants of cytosolic N-terminal protein processing. Eur J Biochem. 1986 Jan 2;154(1):193–196. [PubMed]
  • McGeoch DJ. On the predictive recognition of signal peptide sequences. Virus Res. 1985 Oct;3(3):271–286. [PubMed]
  • Klapper MH. The independent distribution of amino acid near neighbor pairs into polypeptides. Biochem Biophys Res Commun. 1977 Oct 10;78(3):1018–1024. [PubMed]

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