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Nutr J. 2011; 10: 55.
Published online 2011 May 19. doi:  10.1186/1475-2891-10-55
PMCID: PMC3112405
Copyright ©2011 Infante et al; licensee BioMed Central Ltd.
Modification of stool's water content in constipated infants: management with an adapted infant formula
Dámaso D Infante,corresponding author1 Oscar O Segarra,1 Susana S Redecillas,1 Marina M Alvarez,1 and Mar M Miserachs1
11Unit of Paediatric Gastroenterology, Hepatology and Nutrition. Hospital Universitari Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain
corresponding authorCorresponding author.
Dámaso D Infante: dinfante/at/vhebron.net; Oscar O Segarra: osegarra/at/vhebron.net; Susana S Redecillas: sredecil/at/vhebron.net; Marina M Alvarez: maralvarez/at/vhebron.net; Mar M Miserachs: mar/at/miserachs.com
Received July 16, 2010; Accepted May 19, 2011.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Background
Constipation is a common occurrence in formula-fed infants. The aim of this preliminary study was to evaluate the impact of a formula with high levels of lactose and magnesium, in compliance with the official regulations, on stool water content, as well as a parental assessment of constipation.
Materials and methods
Thirty healthy term-born, formula-fed infants, aged 4-10 weeks, with functional constipation were included. All infants were full-term and fed standard formula. Exclusion criteria were preterm and/or low birth weight, organic constipation, being breast fed or fed a formula specially designed to treat constipation. Stool composition was measured by near-infrared reflectance analysis (NIRA) and parents answered questions about crying associated with defecation and stool consistency at baseline and after two weeks of the adapted formula.
Results
After 2 weeks of the adapted formula, stool water content increased from 71 +/- 8.1% to 84 +/- 5.9%, (p < 0.02). There was no significant change in the stool's fat, protein or carbohydrate content. Parental impressions of constipation were improved with the decrease in stool hardness (100% with hard stools at baseline, 10% after 2 weeks), pain with defecation (90% at baseline, 10% after 2 weeks), and the requirement for rectal stimulation to achieve defecation (70% at baseline, 30% after 2 weeks, p < 0.001 for all three indicators).
Conclusions
This preliminary study suggests that an adapted formula with high levels of lactose and magnesium increases stool water content and improves symptoms of constipation in term-born, formula-fed infants. A larger randomized placebo-controlled trial is indicated.
Keywords: constipation, infant, NIRA, lactose, magnesium
Background
Constipation is usually defined in terms of changes in the frequency, size, and consistency of stools or difficulty in defecating [1]. It is a common cause of consultation, and in an observational study of 3487 infants, its prevalence was 7.8% (95 CI 6.9-8.7) [2]. Fewer bowel movements and/or hard stools may result in distension of the colon, pain, irritability and crying in the infant and this may be a cause of infantile colic [3,4]. Chronic constipation is a source of anxiety for parents who worry that a serious disease may cause this symptom [5]. The term "functional constipation" is used when no specific organic cause can be found. For younger infants, laxatives are not used and few infant formulae have been designed to solve the problem and for maintenance therapy.
Near-infrared reflectance analysis (NIRA) is a simple, rapid, reliable method to measure fat, protein, carbohydrate and water content in stools and has been used for over a decade [6-9]. In adults, it has been demonstrated that reduced stool weights in constipated subjects are linked to a reduction in stool water content [10].
The objective of this study is to analyze stool composition and parental assessment of symptoms of constipation with an adapted anti-constipation formula designed to reduce hard stools.
Patients and method
Patients
In the present study, constipation was characterized by the elimination of hard stools and by at least one of the following characteristics: excessive crying at the time of defecation (painful defecation), difficult passage or the need for external help to defecate [11].
Thirty constipated (according to the aforementioned definition) formula-fed infants aged 4-10 weeks were included. No organic cause of constipation was found in any of them. All were fed standard formula prior to enrolment and visited their paediatrician for constipation. All infants were full-term and had a normal birth weight (> 3.100 kg); none had a relevant medical history or pathological neonatal conditions. Preterm and low birth-weight infants were excluded. None were receiving an infant formula specially composed of modified nutrients or laxatives to treat constipation.
After enrolment, all 30 infants were fed Novalac AE (IT) (United Pharmaceuticals SA, France) for 2 weeks. The composition of this formula (Table ​(Table1)1) has been adapted in compliance with EU regulations to ease constipation [12,13]. Stools at baseline and after 2 weeks of the adapted formula were assessed for faecal fat, water, carbohydrates, and protein by NIRA. A questionnaire about crying during defecation, stool consistency and need for help with defecation was filled out by the parents at inclusion and after 2 weeks of the adapted formula. To evaluate stool consistency, the photographic Bristol stool form scale was used: stool hardness is classified from 1 to 7 according to the stool's appearance, in which 1-2 corresponds to hard stools ("like nuts, lumpy"), type 3 and 4 to intermediate, type 5-6 to soft stools and type 7 to completely liquid stools [14].
Table 1
Table 1
Novalac AE (IT) composition
The study was coordinated and conducted by the Gastroenterology unit at Vall d'Hebron Children's Hospital (Barcelona, Spain) and the protocol was approved by the hospital's medical ethics committee (Comitè Ètic Hospital Vall d'Hebron). All parents granted their informed consent to participate in the study.
Methods
The determination of fat, nitrogen, water and carbohydrates in the faeces was done using NIRA (near-infrared reflectance analysis) [6-9]. The NIRA technique is based on measuring radiation in the near-infrared spectrum (FENIR 8820 infrared analyser, Perten, Hamburg, Germany) on the surface of the sample, i.e. the matrix/substrate ratio, and the infrared reflection on the faecal surface at a given wavelength. The components measured belong to certain functional groups (CH, NH, OH) with specific absorption bands in the near-infrared spectrum (700-2500 nm). The spectroscopic response (reflection) of a faecal sample is thus related to the concentration of the components (functional groups). The correlation of the reflection taken at 12 wavelengths is determined using a computerised algorithm.
Prior to the current study, we compared NIRA measurements of stool fat, nitrogen, water and carbohydrate content in eighty stool samples from infants and children to the same measurements performed using traditional methods [6,8,15-17]. The normal values based on our own results, which are comparable to results from other authors, expressed as % (g/100 g of stools) are: fat: < 5%, nitrogen: < 1.8%, carbohydrates: < 2% and water: between 80 and 85%. However, the fat content for formula-fed infants is usually higher during the first 6 months of life (between 7 and 11%) [18,19].
Statistical analysis
Non-parametric tests were used to test for changes in quantitative measurements over time (Wilcoxon sign-rank test) and categorical changes over time (McNemar test). The statistical analysis was performed using the SPSS 18.0 program (SPSS Inc, Chicago, IL).
Results
Clinical data
The results of the NIRA analysis are presented in table ​table2.2. Water content increased significantly (71% at inclusion, 84% after 2 weeks, p < 0.01). Other parameters (fat, nitrogen, carbohydrates) did not change.
Table 2
Table 2
Stool NIRA at inclusion and follow-up (2 weeks).
For all infants, stools were reported as hard at inclusion (Bristol stool form scale type 1 in 23 infants and type 2 in 7 infants.). Defecation was described as painful (crying, irritability) in 90% of them and 70% required help to defecate. At the end of the follow-up period, stools had a soft consistency in 90% of the infants (Bristol stool form scale type 5 in 15 infants and type 6 in 12 infants) (p < 0.0001), 90% presented no pain or discomfort (p < 0.0001) and 70% did not require help to defecate (p < 0.001) (see Table ​Table33).
Table 3
Table 3
Parental assessments
Discussion
Breast-fed infants have significantly more stools per day than formula-fed ones and only a few have hard stools (1.1% of exclusively breast-fed infants vs. 9.2% of formula-fed infants [20,21]). The frequency of painful defecation in constipated children varies from 89% [22] to 40-50% [23,24] depending on the series reported. Fewer bowel movements due to prolonged transit time may result in distension of the gut, irritability and crying, and this may be a reason for inclusion in the associated group of infantile colic [2-4]. Tunc et al. report that, in the first 2 months of life, the median number of stools is lower in colicky infants than in those who do not have colic (p = 0.0001) [18].
Several hypotheses have been proposed to explain the softer stools associated with breast feeding. First, increased levels of gastric inhibitory polypeptide, motilin, neurotensin, vasoactive intestinal peptide secretions in formula-fed infants compared to breast-fed infants may explain their slower intestinal transit [25]. Second, more frequent breast feedings may stimulate the gastro-colic reflex resulting in more frequent defecation. Third, human milk contains large amounts of prebiotic oligosaccharides. Fourth, fat composition may play a role in softer stools. In breast milk and in most infant formulae, fats represent nearly 50% of energy content. The main saturated fatty acid in breast milk is palmitic acid (C16:0), with more than 70% esterified to the sn-2 position, whereas in regular infant formulae 88-94% of the palmitic acid is esterified to the sn-1,3 position. Lipolysis at the sn-1 and sn-3 positions requires pancreatic lipase, which is deficient in the first 6 months of life. The result is relative fat malabsorption, which may react with luminal calcium to form calcium soaps producing hard stools [25,26].
Previous NIRA studies have shown lower stool fat in breast-fed infants: 2.6% in breast-fed infants versus 7-11% in formula-fed infants younger than 6 months and less than 5% in formula-fed infants older than 6 months [9,10]. Our study confirmed the relative steatorrhoea in formula-fed infants [18,19]. Feeding a formula containing a high concentration of sn-2 palmitic acid caused softer stools but no change in stool frequency in one study [19] and decreased fatty acid soaps in another study [27]. We saw no change in stool fat content in the current study, suggesting that the improvement in constipation was not related to changes in fat metabolism.
A clinical trial by Chao et al. of 93 infants (average age 3.8 months) showed that the same formula we used in the current study (Novalac AE/IT) improved infant constipation. The infants receiving this formula had increased stool weight and decreased abdominal distension and irritability compared to infants receiving placebo [28]. Our study adds to these results and includes objective NIRA data.
The two additives to the adapted formula likely impact stool water content through different mechanisms. Lactose has prebiotic effects in that it is not completely absorbed in young infants and stimulates the growth of commensal bacteria. Non-hydrolyzed lactose reaches the colon, where it is metabolized by anaerobic microorganisms, producing an osmotic laxative effect as it attracts water into the intestinal lumen [29-32]. Magnesium, due to its osmotic properties, increases the laxative effect and stimulates intestinal motility by inducing cholecystokinin secretion [33].
In conclusion, this non-randomized, non-placebo-controlled preliminary study demonstrates that the use of an infant formula with high lactose and magnesium concentrations may increase stool hydration, which softens the stools with a corresponding clinical improvement. Thus, constipated infants who present hard stools may benefit from a change from standard formula to this specifically adapted formula. Larger randomized clinical trials on the efficacy of this formula are needed.
Competing interests
The authors declare no competing interests.
Authors' contributions
DI: designed, performed the research, analysed the data and wrote the paper. OS: performed the research. SR: performed the research. MA: performed the research. MM: revised data and wrote the paper. All authors read and approved the final manuscript.
Acknowledgements
The authors wish to thank Bernard Le Luyer for proofreading the manuscript.
The authors wish to thank United Pharmaceuticals for providing free Novalac AE (IT) formula to the participating infants for up to 1 month.
References
  • Baker SS, Liptak GS, Colleti RB, Croffie JM, Di Lorenzo C, Ector W, Nurko S. Constipation in infants and children: evaluation and treatment. J Pediatr Gastroenterol Nutr. 1999;29:612–626. doi: 10.1097/00005176-199911000-00029. [PubMed] [Cross Ref]
  • Infante D, Badia X, Ariño-Armengol B, Villegas V. Prevalence and dietetic management of mild gastrointestinal disorders in milk-fed infants. World J Gastroenterol. 2008;14:248–254. doi: 10.3748/wjg.14.248. [PMC free article] [PubMed] [Cross Ref]
  • Lucassen PL, Assendelft WJ, van Eijk JT, Gubbels JW, Douwess AC, van Geldrop WJ. Systematic review of the occurrence of infantile colic in the community. Arch Dis Child. 2001;84:398–403. doi: 10.1136/adc.84.5.398. [PMC free article] [PubMed] [Cross Ref]
  • Reijneveld SA, Brugman E, Hirasing RA. Excessive infant crying: the impact of varying definitions. Pediatrics. 2001;4:893–897. [PubMed]
  • Constipation Guideline Committee of the North American Society for Pediatric Gastroenterology Hepatology and Nutrition. Evaluation and treatment of constipation in infants and children: recommendations of the North American Society for Pediatric Gastroenterology, Hepatology and Nutrition. J Pediatr Gastroenterol Nutr. 2006. [PubMed]
  • Benini L, Caliari S, Guidi BC, Bretegani MT, Castelani G, Sembenini C, Bardelli E, Vantini I. Near infrared spectroscopy for fecal fat measurement: comparison with conventional gravimetric and titrimetric methods. Gut. 1989;30:1344–47. doi: 10.1136/gut.30.10.1344. [PMC free article] [PubMed] [Cross Ref]
  • Stein J, Purschian B, Bieniek U, Caspary WF, Lembcke B. Validation of near-infra-red reflectance analysis (NIRA) for the assessment of fecal fat, nitrogen and water. A new approach to malabsorption syndromes. Proceedings of the Annual Meeting of the American Gastroenterological Association (AGA), May 10-13, 1992, San Francisco, California. pp. e1–13.
  • Picarelli A, Greco M, Giovambattista F, Ramazzotti A, Cedrone C, Corazziari E, Torsoli A. Quantitative determination of faecal fat, nitrogen and water by means of a spectrophotometric technique: near infrared reflectance analysis (NIRA). Assessment of its accuracy and reproducibility compared with chemical methods. Clin Chim Acta. 1995;234:147–56. doi: 10.1016/0009-8981(94)05993-3. [PubMed] [Cross Ref]
  • Stein J, Purschian B, Zeuzem S, Lembcke B, Caspary WF. Quantification of fecal carbohydrates by near-infrared reflectance analysis. Clin Chem. 1996;42:309–12. [PubMed]
  • Aichbichler BW, Wenzel HH, Santa Ana CA, Porter JL, Schiller LR, Fordtran JS. A comparison of stool characteristic from normal and constipated people. Dig Dis Sci. 1998;43:2353–2362. doi: 10.1023/A:1026699525487. [PubMed] [Cross Ref]
  • Hyams J, Colletti R, Morais MB, Faure C, Gabriel-Martinez E, Maffei HVL, Morais MB, Hock QS, Vandenplas Y. Functional gastrointestinal disorders: Working group report of the First World Congress of Pediatric Gastroenterology, Hepatology, and Nutrition. J Pediatr Gastroenterol Nutr. 2002;35(suppl 2):110–117. [PubMed]
  • European Commission. Scientific Committee on Food. Report of the Scientific Committee on Food on the revision of essential requirements of infant formulae and follow-on-formulae. 2003. SCF/CS/NUT/IF/65 Final.
  • Koletzko B, Baker S, Cleghorn G, Neto UF, Gopalan S, Hernell O. et al. Global standard for the composition of infant formula: recommendations of an ESPGHAN coordinated international expert group. J Pediatr Gastroenterol Nutr. 2005;41:578–579. doi: 10.1097/01.mpg.0000189329.54774.e0. [PubMed] [Cross Ref]
  • Lewis SJ, Heaton KW. Stool form scale as a useful guide to intestinal transit time. Scand J Gastroenterol. 1997;32:920–924. doi: 10.3109/00365529709011203. [PubMed] [Cross Ref]
  • Neumeister V, Henker J, Kaltenborn G, Sprössig C, Jaross W. Simultaneous determination of fecal fat, nitrogen, and water by near-infrared reflectance spectroscopy. J Pediatr Gastroenterol Nutr. 1997;25(4):388–93. doi: 10.1097/00005176-199710000-00005. [PubMed] [Cross Ref]
  • Van den Neucker AM, Forget PP, van Kreel B. Lipid, nitrogen, water and energy content of a single stool sample in healthy children and children with cystic fibrosis. Eur J Pediatr. 2003;162(11):764–6. doi: 10.1007/s00431-003-1291-y. Epub 2003 Aug 27. [PubMed] [Cross Ref]
  • Neucker AV, Bijleveld CM, Wolthers BG, Swaaneburg JC, Kester AD, Kreel B, Forget PP. Comparison of near infrared reflectance analysis of fecal fat, nitrogen and water with conventional methods, and fecal energy content. Clin Biochem. 2002;35(1):29–33. doi: 10.1016/S0009-9120(01)00283-1. [PubMed] [Cross Ref]
  • Tormo R, Infante D. Valoración no invasiva de la administración en recién nacidos y lactantes afectados por cólicos del lactante de una fórmula de inicio y otra de adicionada. Acta Pediatr Esp. 2003;61:601–611.
  • Rivero-Marcotegui A, Olivera-Olmedo JE, Valverde-Visus FS, Palacios-Sarrasqueta M, Grijalva-Uche A, García-Merlo S. Water, fat, nitrogen, and sugar content in feces: reference intervals in children. Clin Chem. 1998;44:1540–1544. [PubMed]
  • Weaver LT, Steiner H. The bowel habit of young children. Arch Dis Child. 1984;59:649–652. doi: 10.1136/adc.59.7.649. [PMC free article] [PubMed] [Cross Ref]
  • Tunc VT, Camurdan AD, Ilhan MN, Sahin F, Beyazova U. Factors associated with defecation patterns in 0-24-month-old children. Eur J Pediatr. 2008;167:1357–1362. doi: 10.1007/s00431-008-0669-2. [PubMed] [Cross Ref]
  • Bongers MEJ, de Lorijn F, Reitsma JB, Groeneweg M, Taminiau JA, Benninga MA. The clinical effect of a new infant formula in term infants with constipation: a double-blind, randomized cross-over trial. Nutr J. 2007;6:8. doi: 10.1186/1475-2891-6-8. [PMC free article] [PubMed] [Cross Ref]
  • Loening-Baucke V. Prevalence, symptoms and outcome of constipation in infants and toddlers. J Pediatr. 2005;146:359–363. doi: 10.1016/j.jpeds.2004.10.046. [PubMed] [Cross Ref]
  • van den Berg MM, van Rossum CH, de Lorijn F, Reitsma JB, Di Lorenzo C, Benninga MC. Functional constipation in infants: a follow-up study. J Pediatr. 2005;147:700–704. doi: 10.1016/j.jpeds.2005.05.032. [PubMed] [Cross Ref]
  • Quinian PT, Lockton S, Irwin J, Lucas AL. The relationship between stool hardness and stool composition in breast- and formula-fed infants. J Pediatr Gastroenterol Nutr. 1995;20:81–90. doi: 10.1097/00005176-199501000-00014. [PubMed] [Cross Ref]
  • Carnielli VP, Luijendijk IHT, van Goudoever JB, Sulkers EJ, Boerlage AA, Degenhart HJ, Sauer PJ. Structural position and amount of palmitic acid in infant formulas: effects on fat, fatty acid, and mineral balance. J Pediatr Gastroenterol Nutr. 1996;23:554–560. [PubMed]
  • Kennedy K, Fewtrell MS, Morley R, Abbott R, Quinlan PT, Wells JC, Bindels JG, Lucas A. Double-blind, randomized trial of a synthetic triacylglycerol in formula-fed term infants: effects on stool biochemistry, stool characteristics, and bone mineralization. Am J Clin Nutr. 1999;70:920–927. [PubMed]
  • Chao HS, Vandenplas Y. Therapeutic effect of Novalac IT in infants with constipation. Nutrition. 2007;23:469–473. doi: 10.1016/j.nut.2007.03.006. [PubMed] [Cross Ref]
  • Lucas A, Sarson DL, Blackburn AM, Adrian TE, Aynsley-Green A, Bloom SR. Breast vs. bottle: endocrine responses are different with formula feeding. Lancet. 1980;1:1267–1269. [PubMed]
  • Barr RG, Hanley J, Patterson DK, Wooldridge J. Breath hydrogen excretion in normal newborn infants in response to usual feeding patterns: evidence for "functional lactase insufficiency" beyond the first month of life. J Pediatr. 1984;104:527–533. doi: 10.1016/S0022-3476(84)80541-7. [PubMed] [Cross Ref]
  • Roggero P, Mosca F, Motta G, Mangiaterra V, Perazzani M, Offredi ML, Marini A, Careddu P. Sugar absorption in healthy preterm and full-term infants. J Pediatr Gastroenterol Nutr. 1986;5:214–219. [PubMed]
  • Laforgia N, Benedetti G, Altavilla T, Baldassarre ME, Grassi A, Bonsante F, Mautone A. Significance of lactose breath test in the newborn. Minerva Pediatr. 1995;10:433–436. [PubMed]
  • Binder HJ, Donowitz M. A new look at laxative action. Gastroenterology. 1975;69:1001–1005. [PubMed]
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