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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Neurobiol Learn Mem. Author manuscript; available in PMC Jul 1, 2012.
Published in final edited form as:
PMCID: PMC3111759
NIHMSID: NIHMS275794
Emerging roles of epigenetic mechanisms in the enduring effects of neonatal stress and experience on learning and memory
Shawn McClelland,* Aniko Korosi,* Jessica Cope, Autumn Ivy, and Tallie Z. Baram
Departments of Anatomy/Neurobiology, Pediatrics and Neurology, UC-Irvine, Irvine, CA, 92697 USA
Corresponding author: Tallie Z. Baram, MD, PhD, tallie/at/uci.edu, Tel.: +1-949-824-1063; fax: +1-949-824-1106;, Address: Med Sci I; ZOT 4475, University of California at Irvine, Irvine, California 92697-4475, USA
*Equal contributors
Epigenetic mechanisms are involved in programming gene expression throughout development. In addition, they are key contributors to the processes by which early-life experience fine-tunes the expression levels of key neuronal genes, governing learning and memory throughout life. Here we describe the long-lasting, bi-directional effects of early-life experience on learning and memory. We discuss how enriched postnatal experience enduringly augments spatial learning, and how chronic early-life stress results in persistent and progressive deficits in the structure and function of hippocampal neurons. The existing and emerging roles of epigenetic mechanisms in these fundamental neuroplasticity phenomena are illustrated.
Keywords: CRH, epigenetic, hippocampus, neonatal, early-life experience, maternal care, learning and memory, stress, corticotropin releasing hormone, glucocorticoids, GR, neuroplasticity, programming, resilience
Numerous clinical reports demonstrate a strong association between early-life experience and subsequent cognitive functions. Chronic childhood stress (such as extreme poverty, loss of parent, social deprivation or abuse) correlates with learning and memory impairments later in life (Kaplan et al., 2001; Nelson et al., 2007; Wilson et al., 2007). As examples, lower socioeconomic level early in life correlates with cognitive function in adulthood, and post-institutionalized orphans have abnormal neuronal function in limbic areas including the hippocampus, as shown by functional MRI studies (Chugani et al., 2001), and by worse cognitive performance when compared to never-institutionalized children (Nelson et al., 2007). Improving the experience of these institutionalized infants by placing them in families significantly improves learning and memory long-term. Remarkably, the timing of the placement into foster care is crucial, and placement before the age of two years is associated with improved outcome (Bos, Zeanah, Smyke, Fox, & Nelson, 2010). These data suggest that critical developmental periods exist for the processes by which early-life experience shapes cognitive function throughout life.
The impact of early-life experience, and especially of chronic stress, on the integrity of the hippocampus, a region subserving certain learning and memory processes (Andersen, Moser, Moser & Trommald, 1996; Eichenbaum, Yonelinas, & Ranganath, 2007; Morris et al., 2003; Squire, Wixted, & Clark, 2007), is supported also by clinical studies suggesting that hippocampal volumes in adults that have experienced early-life abuse are smaller (Bremner, et al., 1997). Whereas this view is not universally endorsed (Lenze, Xiong, & Sheline, 2008; Lyons, Yang, Sawyer-Glover, Moseley, & Schatzberg, 2001), this association and similar findings in other human studies (Buss et al., 2007) suggest that chronic early-life stress is associated with impairments in hippocampal structure and function in adulthood. In addition these correlational studies demonstrate the complexity of human research: elucidating the potential causal relationship between early-life stress and later life cognitive outcomes is difficult, because of uncontrollable variables such as genetics and subtle environmental influences that may not be measurable. Such studies lead to the realization that use of animal models benefits our understanding of the causal relationship between early-experiences and life-long learning and memory. These enable prospective longitudinal studies as well as control of genetic background (Nestler & Hyman, 2010). In addition, parameters of interest can be manipulated and subsequent experiences can be controlled throughout the entire period of investigation.
Notably, uncovering the biological mechanisms involved in the long-term consequences of enhanced early-life experience is of paramount importance, because these mechanisms may be employed for therapeutic interventions and improved outcome. This has happened, for example, when infant position was found to govern sudden infant death, and care of infants was changed (Ponsonby, Dwyer, Gibbons, Cochrane, & Wang, 1993). In addition, discovering if chronic early-life stress directly impacts cognitive function is extremely important because over 50% of the world’s children are exposed to chronic stress (UNICEF, 2005), and such stress cannot currently be prevented. Therefore, establishing causality and defining the molecular and cellular mechanisms for potential long-lasting effects of early-life experience and stress on learning and memory are prerequisites to preventive and therapeutic approaches in the future.
For the past six decades, scientists have employed models in primates and rodents to manipulate environmental and genetic variables for the study of early-life experience on later-life cognitive functions (for review, see Korosi & Baram, 2009; Levine, 2000). In these animal models, early-life experience was modulated in a bi-directional manner (Figure 1). Acute-intermittent stress, such as daily maternal separation, or chronic stress imposed via alteration of maternal behavior, were designed to mimic human conditions of poverty, illness or neglect/ abuse (Avishai-Eliner, Brunson, Sandman, & Baram, 2002; Brunson, Chen, Avishai-Eliner, & Baram, 2003; Fenoglio, Brunson, & Baram, 2006a; Heim, Plotsky, & Nemeroff, 2004; Levine, 2000). In contrast, enhanced early-life experience was generated via a naturalistic selection of high-caring dams (Hofer, 1994), or via procedures, such as brief daily handling of the pups, that augment maternal care and thus maternal-derived sensory input (Brunson, Avishai-Eliner, Hatalski, & Baram, 2001; Fenoglio, Chen, & Baram, 2006b; Korosi & Baram, 2009; Korosi et al., 2010; Meaney, Aitken, van Berkel, Bhatnagar, & Sapolsky, 1988).
Figure 1
Figure 1
Bidirectional effects of early-life experience on cognitive function throughout life
A majority of studies employing these structured alterations of early-life experience have focused on ‘emotional’ outcomes- the vulnerability or resilience of the adult ‘graduates’ of these early-life manipulations to depressive-like or anxiety-like behaviors. However, existing and emerging evidence indicates that early-life experience and stress also contribute significantly to learning and memory throughout life, as suspected from the clinical correlational studies of infants and children. In this paper, we review data that support a causal relationship of enhanced early-life experience (derived from augmented quality of maternal care) and learning and memory function during adulthood. We also discuss the causal relationship of chronic stress during the neonatal / infancy period and cognitive decline commencing in middle age. In both cases we describe the molecular and cellular processes that are involved, and discuss the role of epigenetics in the persistently altered expression of key genes that contribute to these phenotypes.
A key determinant of early-postnatal existence involves the interaction of the immature individual with his / her parent(s). Both clinical and experimental studies have confirmed the fundamental role of the presence and sensory input from a mother on the essence of the neonatal and infancy experience. Considered along a continuum, frequent and consistent nurturing care suppresses stress in the immature rat, monkey and human (Dent, Smith, & Levine, 1999; Gunnar, Larson, Hertsgaard, Harris, & Brodersen, 1992; Harlow & Suomi, 1971), whereas absence of the mother or abnormal quality or quantity of maternal care is a major provoker of the newborn / infant “stress system” (Figure 1). Based on this body of information, many manipulations of early-life experience have utilized modulation of mother-infant interactions. In addition, the principal ‘read-outs’ of the effects of these modulations on the infant brain have included acute and persistent alterations in the expression and function of genes that are involved in regulation of the stress response (Avishai-Eliner, Eghbal-Ahmadi, Tabachnik, Brunson, & Baram, 2001a; Fenoglio et al., 2005; Korosi et al., 2010; Plotsky & Meaney, 1993; Weaver et al., 2004).
As shown in Figure 2, the response to stress is governed by a number of neurotransmitters, neuromodulators and steroids (Joels & Baram, 2009; McEwen, 1999; Ulrich-Lai & Herman, 2009). In essence, external (or internal) signals that are interpreted as indicating potential or existing threat result in release of the neuropeptide corticotropin releasing hormone (CRH) from cells within the hypothalamus (Vale, Spiess, Rivier, & Rivier, 1981), as well as within the hippocampus (Chen, Bender, Frotscher, & Baram, 2001a; Chen et al., 2004b; Chen, Fenoglio, Dube, Grigoriadis, & Baram, 2006; Chen et al., 2010), and the amygdala (Roozendaal, Brunson, Holloway, McGaugh, & Baram, 2002). Hypothalamic CRH reaches the pituitary gland releasing corticotropin, and the latter promotes secretion of glucocorticoids. Glucocorticoids prepare the body for stress but, importantly, cross the blood brain barrier and interact with cognate receptors throughout the brain including in principal cells of the hippocampal formation (McEwen, 1999; Reul & de Kloet, 1985). Neurons within the hippocampal formation are activated by salient stresses, as measured by immediate early-gene expression and a number of electrophysiological parameters (Alfarez, Joels, & Krugers, 2003; Kim & Diamond, 2002; Pavlides, Watanabe & McEwen, 1993; Chen, Fenoglio, Dube, Grigoriadis, & Baram, 2006) reviewed by (Joels & Baram, 2009). In contrast, pleasurable or non-stressful experiences often cause minimal and rapidly-decaying release of stress hormones with transient activation of distinct cellular and molecular cascades (Feder, Nestler, & Charney, 2009; Fenoglio et al., 2006b; Sweatt, 2009). Whereas these processes were first uncovered in the adult organism, they function also in the neonatal period (Brunson et al., 2003; Dallman, 2000; Gunnar & Quevedo, 2007). Specifically, stress and other experiences activate hypothalamic (Chen, Hatalski, Brunson & Baram, 2001b) and hippocampal (Hatalski, Brunson, Tantayanubutr, Chen, & Baram, 2000) neurons. CRH is released from the hypothalamus to induce release of peripheral hormones (Yi & Baram, 1994), and CRH and glucocorticoid receptor (GR) signaling are functional in the periphery and within the hippocampus (Avishai-Eliner, Brunson, Sandman, & Baram, 2002; Lupien, McEwen, Gunnar, & Heim, 2009; Rosenfeld, van Eekelen, Levine, & de Kloet, 1993; Yi, Masters & Baram, 1993).
Figure 2
Figure 2
A schematic of the sequence of cells and molecules activated during stress, and their effects on the brain
The ‘tone’ of the system, i.e., the magnitude of the neuronal and hormonal response to a given stressor, is governed, at least in part, by levels of expression of key ligands and receptors (Heim et al., 2009; Holsboer & Ising, 2008). CRH levels in hypothalamus and in the hippocampus are typically correlated with magnitude of release during stress (Chen et al., 2004b; Kovacs & Sawchenko, 1996). GR levels in hippocampus are generally correlated with efficient shut-off of the stress response, via negative feedback, as well as with resilience to depression and other stress-related disorders (Holsboer, 2001; McEwen, 1999; Schmidt et al., 2005). Thus, augmented early-life experience has been found to be associated with enduring attenuation of stress response, as well as augmented learning and memory (Fenoglio et al., 2005; Meaney et al., 1991). At the molecular level, reduction of the expression of CRH in the hypothalamus and increased expression of GR in hippocampus have been documented (Plotsky & Meaney, 1993). In contrast, as will be described below, chronic early stress has recently been found to upregulate expression of CRH in hippocampus (Ivy et al., 2010). Taken together, these data suggest that, among the many enduring changes in the expression of key neuronal genes (Roth, Lubin, Funk, & Sweatt, 2009), persistent alteration in the expression levels of genes governing the stress-response, specifically GR and CRH, might contribute crucially to enduring phenotypic alteration of learning and memory that are induced by early-life experience and stress. The mechanism by which these changes are initiated and the bases of their persistence involve epigenetic processes (Bale et al., 2010; Borrelli, Nestler, Allis, & Sassone-Corsi, 2008).
Enriched early-life experience during the first week(s) of life leads to a phenotype of improved hippocampus-dependent learning and memory (assessed using the Morris water maze and the novel object recognition tests) that last throughout adulthood (Fenoglio et al., 2005; Korosi & Baram, 2009; Meaney, 2001; Figure 3 left panel). What might the cellular and molecular basis of this enduring effect be? When the early postnatal experience is enriched by augmented sensory input from the mother, hypothalamic CRH expression is repressed and glucocorticoid receptor expression in hippocampal area CA1 is augmented, and both of these changes appear to be life-long. Time course analyses demonstrated that CRH repression (Figure 3 right panel), and the resulting chronic reduction in stress-induced release of hypothalamic CRH and of plasma glucocorticoid levels preceded changes in GR expression (Avishai-Eliner et al., 2001a). In addition, blocking the binding of CRH to its receptor in non-enriched pups led to improved spatial learning in these non-enriched animals as adults (Fenoglio, et al., 2005). Low plasma glucocorticoid levels are the major inducer of GR expression in hippocampus (Herman & Spencer, 1998), so the above data together suggest that the enduring changes in gene expression set in motion by enriched early-life experience might commence at the level of regulation of the Crh gene within the hypothalamus (Korosi et al., 2010; Weaver et al., 2004; Figure 3). If so, then how might the Crh gene be regulated persistently, and by what putative epigenetic processes?
Figure 3
Figure 3
Augmented spatial learning after enriched early-life experience correlates with early and enduring repression of CRH expression in hypothalamus
The Crh gene is composed of two exons and an 800bp intron (Figure 4). The entire protein-coding region is contained in the second exon. The promoter region of the Crh gene contains several putative cis-regulatory elements (Figure 4 top panel) including a cAMP response element (CRE), AP-1 sequence and glucocorticoid response element (GRE), and these contribute to tissue-specific (Seasholtz, Thompson & Douglass, 1988; Vamvakopoulos, et al., 1990), circadian (Watts, Tanimura, & Sanchez-Watts, 2004) and stress-provoked (Hatalski & Baram, 1997; Shepard, Liu, Sassone-Corsi, & Aguilera, 2005) expression of this gene. There are a number of CpG sequences in both the promoter region and in the first intron (Elliott, Ezra-Nevo, Regev, Neufeld-Cohen, & Chen, 2010; McGill et al., 2006; Mueller & Bale, 2008; Figure 4 bottom panel). Indeed, the Crh gene is regulated also by methyl CpG-binding protein 2 (MeCP2; Chahrour et al., 2008; McGill et al., 2006) a transcriptional repressor that binds methylated CpG dinucleotides and recruits corepressors and chromatin remodeling proteins (Nan et al., 1998).
Figure 4
Figure 4
Architecture and epigenetic changes in the Crh gene
In addition, the Crh gene intron contains a functional restrictive silencing element-1/neuron restrictive silencing element (RE-1/NRSE) sequence, that binds restrictive element silencing transcription factor/neuron restrictive silencing factor (REST/NRSF; Seth & Majzoub, 2001). Whereas NRSF was originally described in non-neuronal cells, where it restricts neuronal gene expression, the repressor is now known to be expressed in neurons (Calderone, et al., 2003; Palm, Belluardo, Metsis, & Timmusk, 1998) and to regulate neuronal gene expression (Andres et al., 1999; Gillies, Haddley, Vasiliou, Bubb, & Quinn, 2009; Korosi et al., 2010; Seth & Majzoub, 2001; McClelland et al., 2011, in revision). NRSF functions by recruiting chromatin-modifying cofactors (Naruse, Aoki, Kojima, & Mori, 1999; Roopra, Huang, & Dingledine, 2001; Roopra et al., 2000; Zheng, Zhao, & Mehler, 2009).
The specific epigenetic mechanisms by which early-life experience attenuates CRH expression acutely and long-term are not fully elucidated. At the end of the enriched-experience epoch (postnatal day 9), NRSF levels have been found to be augmented in hypothalamic tissue, and the repressor was bound to the Crh gene chromatin, as found using chromatin immunoprecipitation (Korosi et al., 2010). Both of these findings support a role for NRSF in an epigenetic down-regulation of CRH expression that initiates augmented spatial learning for life (Fenoglio et al., 2005; Korosi et al., 2010; Figure 3). In this study, methylation of CpG dinucleotides of the Crh gene promoter was modestly yet significantly lower in rats immediately following enriched early-life experience (P9) than in controls(Figure 4). Notably, reduced methylation is generally associated with enhanced transcription, whereas Crh gene expression was reduced in this group that performed better in learning and memory tests later in life. Thus, whereas altered methylation of the Crh gene after early-life (Mueller & Bale, 2008) or adult manipulations is clearly operant, its role in the effects of early-life enriched experience on lifelong memory function remains to be fully studied. Additional remaining questions include the nature of the ‘pathways’ from the maternal-derived sensory input to the hypothalamus (Fenoglio et al., 2006b; Korosi & Baram, 2009), and the nature of the signals that converge on the CRH-expressing hypothalamic neuron and provoke it to initiate epigenetic mechanisms that suppress the Crh gene. It has already been established that enriched early-life experience results in reduced numbers of excitatory synapses and excitatory input to CRH-expressing hypothalamic neurons, without altered GABAergic neurotransmission (Korosi, et al., 2010), and this phenomenon might be the signal that sets in motion the epigenetic machinery within the cell (Korosi et al., 2010).
As mentioned above, epidemiological and experimental evidence suggests that adverse early-life experience, and particularly chronic psychological stress, may predispose to cognitive dysfunction (Ammerman, Kolko, Kirisci, Blackson, & Dawes, 1999; Huot, Plotsky, Lenox, & McNamara, 2002; Kaplan et al., 2001; Meaney et al., 1988; Poeggel et al., 2003; van Oers, de Kloet, & Levine, 1999) that appears much later in life. This implies that early-life stress can have effects that impact neuronal function significantly and enduringly throughout adulthood and aging.
The hippocampus is critical for a variety of memory processes (Hollup, Kjelstrup, Hoff, Moser & Moser, 2001) and is unusually vulnerable to stress (Bremner et al., 1997; Kim & Diamond, 2002; McEwen, 1999; Sapolsky, 2002). The bases for this vulnerability include high expression of glucocorticoid receptors, for example in CA1 pyramidal cells (Herman, Patel, Akil, & Watson, 1989; de Kloet, Karst, & Joels, 2008), direct and powerful afferent input from stress-activated brain regions (see Segal, Richter-Levin, & Maggio, 2010; Ulrich-Lai & Herman, 2009; Joels & Baram, 2009 for review), and stress-induced release of endogenous neuromodulators within the hippocampus including CRH (Chen et al., 2004b; Chen et al., 2006; Chen et al., 2010). This susceptibility of hippocampal function to stress likely plays an adaptive role, by enhancing synaptic plasticity (Blank, Nijholt, Eckart, & Spiess, 2002) and learning and memory during acute stress that lasts for seconds to minutes (Joels & Baram, 2009). However, it also renders the hippocampus vulnerable to potentially deleterious effects of severe or chronic stress (McEwen, 1999; Brunson et al., 2003). Chronic stress (or long-term elevation of plasma stress hormones) in adult and aging rats, affects hippocampus-dependent cognitive function (Alfarez et al., 2003; Bodnoff et al., 1995; Dachir, Kadar, Robinzon, & Levy, 1993; Kerr, Campbell, Applegate, Brodish, & Landfield, 1991; Kim & Diamond, 2002; Landfield, McGaugh, & Lynch, 1978; Luine, Villegas, Martinez, & McEwen, 1994), but these disturbances are generally transient (Pavlides, Nivon, & McEwen, 2002). In contrast, as mentioned above, clinical studies have suggested that when occurring early in life, chronic stress might impact learning and memory in hippocampus in an enduring and potentially progressive manner (Wilson et al., 2007).
To examine if a causal relationship exists between chronic early-life stress and enduring and progressive deficits in learning and memory, and to better understand the mechanisms by which such stress might impact cognitive function long-term, a wide variety of experimental manipulations during development have been designed. These stresses have included acute / intermittent separation of dams from the pups (de Kloet, Oitzl, & Joels, 1999; Huot et al., 2002; Oomen et al., 2009; Oomen et al., 2010; Schmidt, Oitzl, Levine, & de Kloet, 2002; van Oers et al., 1999), a single prolonged separation of dams from the pups (Avishai-Eliner, Yi, Newth, & Baram, 1995; Dent, Smith, & Levine, 2000; Levine, 2000), and a chronic alteration of maternal behavior, resulting in stress to the pups (Brunson et al., 2005; Gilles, Schultz, & Baram, 1996; Ivy et al., 2010; Roth & Sullivan, 2005). The timing of the stress has also been an important variable, with some groups aiming to recreate prenatal stress, and others focusing on the immediate neonatal period, or slightly later. In view of this diversity of approaches and of endpoints, it is remarkable that the results have generally been in accord: early-life stress has adverse effects on spatial learning and these results endure, or emerge later in life (Brunson et al., 2005; Huot et al., 2002). Aiming for a naturalistic rodent model of chronic early-life stress, a paradigm involving maintaining the dam with the pups within an impoverished cage environment was designed, and this manipulation led to chronic stress in both dams and pups (Avishai-Eliner, Gilles, Eghbal-Ahmadi, Bar-El, & Baram, 2001b; Brunson et al., 2005; Ivy, Brunson, Sandman, & Baram, 2008; Rice, Sandman, Lenjavi, & Baram, 2008). In essence, chronic stress was generated during postnatal days 2-9, a period when hippocampal development is comparable to that found late in human gestation and the early postnatal period (Avishai-Eliner et al., 2002). For the stress group, cage environment and maternal behavior were altered by placing pups and dams in cages with limited nesting / bedding material on postnatal day 2. Cages were fitted with soft plastic mesh bottom, bedding was essentially removed, and nesting material consisted of one paper towel that was used by the dam to construct a rudimentary nest area. The abnormal cage environment prevented the dam from constructing satisfactory nests. This resulted in maternal stress, promoting fragmented and erratic care to the pups (Ivy et al., 2008). The disrupted maternal care led to chronic stress in the pups, as measured both by plasma glucocorticoid levels and by the presence of hypertrophied adrenal glands (Avishai-Eliner et al., 2001b; Gilles et al., 1996). This model, where stress was constant and persistent, has been adapted to the mouse (Rice et al., 2008; Schmidt et al., 2002) and has been adopted and modified by several groups around the world (Moriceau, Roth, & Sullivan, 2010; Moriceau, Shionoya, Jakubs, & Sullivan, 2009; Roth & Sullivan, 2005; Schmidt, Wang,& Meijer, 2010).
At the end of the stress period, dams and pups were moved to normal-bedded cages. Maternal behavior normalized within hours, and by the time they reached adulthood, the neuroendocrine parameters of the stress system returned to baseline in the early-life stress graduates, and were indistinguishable from those of conventionally raised rats (Brunson et al., 2005). However, despite apparent dissipation of the physiological correlates of stress, the early-life chronic stress led to enduring and profound changes of the structure and function of hippocampal neurons that are likely generated via epigenetic modulation of key stress hormone genes.
Functionally, young-adult graduates of chronic early-life stress performed reasonably well in the Morris water maze (MWM) test of spatial learning and memory. Long-term potentiation (LTP) in response to high frequency stimulation was normal in both areas CA1 and CA3, though subtle changes in the properties of CA3 pyramidal cells were apparent (Brunson et al., 2005). However, by 7–10 months, performance in the MWM as well as in the novel object recognition test declined in comparison to age-matched controls (Figure 5 left panel). Performance in the MWM might be confounded in rats that had experienced stress early-in life, because the MWM includes adverse elements (forced swim), and it could be argued that the ability of the early-stressed group to ‘cope’ with this additional stress was modulated by the early-life experience. Therefore, an independent memory test that interrogates the hippocampal-limbic circuit and is relatively devoid of intrinsic ‘stress’ was also employed. Performance in the novel object recognition test was also impaired in early-stress rats and this was accompanied by profound attenuation of LTP in both CA3 and CA1 (Figure 5 right panel). The structural correlates of these spatial and recognition memory defects included impoverishment (atrophy) of apical dendritic trees of CA1 and CA3 pyramidal neurons, with commensurate loss of dendritic spines and synapses (Brunson et al., 2005; Ivy et al., 2010; Figure 6 left panel) in the stratum radiatum, the site of commissural/ associational synapses. Thus, a single week of chronic stress early in the postnatal period led to enduring and potentially progressive disturbances in synaptic plasticity and in memory processes, at least in part via loss of dendrites, dendritic spines and excitatory synapses (Chen et al., 2010; Ivy et al., 2010).
Figure 5
Figure 5
Reduced spatial learning and memory skills and attenuated long term potentiation in middle aged rats that had experienced chronic early-life stress
Figure 6
Figure 6
Dendritic atrophy and enduring upregulation of CRH expression in hippocampus of middle-aged rats experiencing chronic early-life stress
The chain of events that bridges a single neonatal week of chronic stress with enduring and progressive disturbances of hippocampal structure and function are not yet fully understood. Because, as described above, stress involves the release and subsequent expression of specific mediators, including neurotransmitters, neuropeptide and steroid hormones (Joels & Baram, 2009; McEwen, 1999; Ulrich-Lai & Herman, 2009), it has been a general assumption that these stress mediators were involved in the processes by which stress impacts learning and memory. Indeed, much work has identified the actions of glucocorticoid receptor activation in the consequences of early stress on cognitive function (Alfarez et al., 2009). However, a key enigma remains: how does a transient elevation of stress hormones result in enduring or progressive disturbance in learning and memory? In the paragraphs below, we describe recent studies that support the idea that chronic early-life stress results in epigenetic ‘programming’ of stress-related genes, including hippocampal CRH expression, at higher levels. The enduring elevation of CRH expression and release impairs dendritic spines, with eventual dying back of dendrites. Loss of dendritic spines and the synapses that they carry might thus underlie the loss of synaptic plasticity and memory function in ‘graduates’ of early-life stress (Figure 5, Figure 6 left panel).
Ivy (2010) found a major increase in the protein levels of CRH in hippocampal pyramidal cells of middle-aged rats stressed early in life (Figure 6 right panel), and similar augmentation was apparent at the mRNA levels (Fenoglio et al., 2006a). In addition, the group was able to block the consequences of chronic early-life stress by interfering with the interaction of CRH with its receptor, CRHR1 within the brain (Ivy et al., 2010). Hippocampal CRH is released during stress throughout life (Chen et al., 2001a; Chen et al., 2004b), and interacts with CRHR1 to cause rapid retraction of dendritic spines (Chen, Dube, Rice, & Baram, 2008; Chen et al., 2010). Exposure of hippocampal organotypic cultures to stress levels of CRH for a week not only reduced spine number, but also led to dying back of dendrites (Chen et al., 2004a). Thus, exposure of the developing hippocampus to stress-levels of CRH during the week of early-life stress, and / or release of pathological levels of the peptide throughout the months that follow might contribute to the loss of structural integrity of hippocampal neurons that characterizes adult rats after chronic early-life stress. This loss of dendrites, spines and synapsesseems to underlie the disturbances of learning and memory in the same rats (Chen et al., 2008; Chen et al., 2010; Ivy et al., 2010).
This scenario raises the obvious question regarding the nature of the mechanism that increase the ‘set-point’ of hippocampal CRH expression throughout the life of early-stressed rats. Based on a large body of work (Bale et al., 2010; Borrelli et al., 2008; Franklin et al., 2010; Haggarty et al., 2010; McGill et al., 2006; Mueller & Bale, 2008; Sweatt, 2009), epigenetic mechanisms are excellent candidates for this enduring, augmented expression of the Crh gene in hippocampus. For example, regulation of DNA methylation (Levenson & Sweatt, 2006) and of histone acetylation and methylation (Gupta et al., 2010) has been found to play key roles in fundamental learning and memory processes subserved by the hippocampal network. Obviously, disruption of such processes by stress would impair cognitive performance. However, whereas there is clear evidence for the involvement of epigenetic regulation in processes of learning and memory in the mature hippocampus (Abel & Zukin, 2008; Fischer, Sananbenesi, Mungenast, & Tsai, 2010; Miller, Campbell, & Sweatt, 2008; Na & Monteggia, 2010; Stefanko, Barrett, Ly, Reolon, & Wood, 2009; Sweatt, 2009), the involvement of such mechanisms in the upregulation of the Crh gene is under active study (Figure 4).
This review focused on the role of transcriptional, and likely epigenetic regulation of the CRH gene in hypothalamus and hippocampus, and the consequences of these changes in gene expression on learning and memory. Two questions that arise are (1) are these changes selective to CRH? and (2) why regulation of CRH rather than other stress-related genes?
The answers to both these questions are speculative at this point. As mentioned above, early-environment-induced resilience to stress is associated not only with reduced CRH expression in hypothalamus, but also in augmented glucocorticoid receptor expression in hippocampus (Plotsky & Meaney, 1993; Avishai-Eliner et al., 2001a). Temporally, the reduction in CRH expression precedes, and might initiate (Fenoglio et al., 2005), the changes in GR. Unlike the resilience to drug abuse, where large-scale changes in gene expression have been delineated (Feder, Nestler, & Charney, 2009), the extent of epigenetic changes of families of genes that might govern the observed enhancement of hippocampal function after enriched early-life environment remain to be fully studied. Weaver et al. identified over 900 genes with altered expression in hippocampus of adult rats that have experienced augmented maternal care early in life (Weaver, Meaney, & Szyf, 2006). Interestingly, the group found reversal of these alterations of gene expression upon treatment with trichostatin or with a source of methyl group (Weaver et al., 2005; Weaver et al., 2006). Whereas the transcriptome arrays were carried out only in adulthood, these data support the idea that epigenetic regulation of the expression of numerous genes takes place after enriched experience early in life that promotes learning and memory long-term.
As mentioned above, levels of stress hormone (and receptor) gene expression are both influenced by stress, and govern the release and / or signaling of stress mediators in hippocampus and throughout the brain. Interestingly, enduring changes in the expression of receptors to systemic stress hormones (GR and MR) have not been observed after early-life stress that promotes deterioration in learning and memory later in life. This observation is intuitively logical, because this type of adaptation would be expected to result in major changes in the body’s response to stress in general, including disruption of the negative feedback by which the hippocampus restrains the hormonal response to stress (Ulrich-Lai & Herman, 2009). In contrast, CRH belongs to a class of modulators, neuropeptides, that play less prominent roles in the peripheral hormonal response (Joels & Baram, 2009). Similar to other peptides, CRH has a peripheral role in mediating the body’s response to stress and, in parallel is released in hippocampus to modulate hippocampal function during stress. Peptides with similar dual roles include ghrelin (Diano et al., 2006) and somatostatin (Bloom, 1986). These peptides, including CRH, are released in specific brain regions and modulate the function of a limited number of neurons within selective populations and networks. In the temporal domain, peptides bridge neurotransmitter function (seconds), and hormonal effects (up to hours). Thus, selective epigenetic regulation of hippocampal CRH expression enriches the repertoire of hippocampal plasticity (Joels & Baram, 2009), enabling augmented fine-tuning of hippocampal function in the context of graded stress situations.
Early-life experience influences learning and memory processes throughout life in a bidirectional manner. The mechanisms for such enduring neuroplasticity include stable alteration in the expression of key neuronal genes, including those that regulate the salience and stressfulness of experience. Enriched postnatal experience enduringly augments learning and memory, at least in part via persistent suppression of CRH expression. In contrast, chronic early-life stress results in long-lasting and progressive deficits in the structure and function of hippocampal neurons, potentially via epigenetic programming of the expression ‘set-point’ of the same gene in hippocampus at a higher level. Many questions remain about the initiation and nature of the epigenetic mechanisms that are involved in programming the enduring effects of early-life experience on learning and memory throughout life.
Acknowledgments
Supported by NIH grants NS28912; MH 73136. We thank Mrs. B. Cartwright for editorial support.
Footnotes
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