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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
FEBS Lett. Author manuscript; available in PMC Nov 6, 2011.
Published in final edited form as:
PMCID: PMC3109430
NIHMSID: NIHMS296545

Recognition of an ERAD-L substrate analyzed by site-specific in vivo photocrosslinking

Abstract

Misfolded, luminal endoplasmic reticulum (ER) proteins must be recognized before being degraded by a process called ERAD-L. Using site-specific photo-crosslinking in S. cerevisiae, we tested luminal interactions of a glycosylated ERAD-L substrate with potential recognition components. Major interactions were observed with Hrd3p. These are independent of the glycan and of other ERAD components, and can occur throughout the length of the unfolded substrate. The lectin Yos9p only interacts with a polypeptide segment distant from the degradation signal. Hrd3p may thus be the first substrate-recognizing component. Der1p appears to have a role in a pathway that is parallel to that involving Hrd3p.

Keywords: ER-associated degradation, misfolded proteins, Hrd3p, Yos9p, Der1p, endoplasmic reticulum

1. Introduction

Proteins that misfold in the lumen of endoplasmic reticulum (ER) are retro-translocated across the ER membrane into the cytosol, poly-ubiquitinated, and ultimately degraded by the proteasome [1, 2]. The process is known as luminal ER-associated protein degradation (ERAD-L) [35]. In S. cerevisiae, ERAD-L requires a hetero-tetrameric membrane protein complex, the Hrd1 complex, which consists of Hrd1p, Hrd3p, Usa1p, and Der1p [35]. Hrd1p is a ubiquitin ligase that spans the ER membrane six times and has a cytosolic RING finger domain [68]. Hrd3p is a single-spanning protein with a luminal domain that recruits the lectin Yos9p. Usa1p spans the membrane twice with N- and C-termini in the cytosol. Finally, Der1p spans the membrane four times. Recent evidence suggests that Hrd1p is the central component of the complex, required for moving misfolded proteins across the membrane [69]. To do so, Hrd1p needs to oligomerize, which is facilitated by Usa1p [911].

How misfolded proteins are recognized and distinguished from folding intermediates is only poorly understood. One mechanism by which misfolded glycoproteins are recognized is through a terminal α1,6-mannose residue in a carbohydrate chain [2, 1214], which is generated by the glycosidase Htm1p when a misfolded protein spends excessive time in the ER. The α1,6-mannose residue is specifically recognized by the lectin Yos9p [12, 15]. However, this cannot be the only recognition mechanism, as the same α1,6-mannose signal does not trigger degradation when placed at other locations in the polypeptide chain [2]. It has therefore been proposed that the unstructured nature of the polypeptide segment around the carbohydrate attachment site serves as an additional signal [16]. Both Yos9p and the luminal domain of Hrd3p have been implicated in recognizing these unfolded polypeptide segments [1719], but evidence for direct interactions is lacking. In addition, it is uncertain whether Yos9p and Hrd3p are the only components of the Hrd1p complex involved in substrate recognition. Transfer of substrate to Hrd1p is only completely abolished when both Yos9p and Der1p are absent, suggesting that there is an additional pathway of substrate delivery involving Der1p [9, 20].

Here we use an in vivo site-specific photocrosslinking method to analyze interactions of an ERAD-L substrate with the potential recognition proteins Hrd3p, Yos9p, and Der1p.

2. Materials and Methods

Yeast Strains and Plasmids

Standard PCR-based recombination methods were used to make gene deletions and to tag proteins [21]. Strains with multiple gene deletions and genomically encoded protein tags were made either by PCR-based homologous recombination or by crossing haploid cells of opposite mating types, followed by sporulation and tetrad dissection [22]. The strains used were isogenic to FY251 (Mata ura3-52 his3Δ200 leu2Δ1 trp1Δ63). Supplementary Tables 1 and 2 list the yeast strains and plasmids used, respectively.

Site-specific in vivo photocrosslinking

Yeast cells were transformed with two plasmids, one encoding a CPY*-derived construct with an amber codon at a selected position, and another encoding both a tRNA that suppresses the amber stop codon and a modified tRNA-synthetase that charges this tRNA with photoreactive benzoyl amino acid [23, 24]. The protocol for photocrosslinking and analysis by immunoblotting has been described [9].

3. Results

To analyze interactions of a luminal misfolded protein, we employed a shortened version of the well-characterized ERAD-L substrate CPY* (sCPY*). This protein contains the last 180 amino acids, including the N-glycosylation site that is part of the degradation signal. This protein is degraded more rapidly than the original CPY* but requires the same ERAD-L components [9]. We also used a version of sCPY* in which dihydrofolate reductase (DHFR) is fused to the C-terminus (sCPY*-DHFR), which slows the degradation of the protein [9, 17]. Both sCPY* and sCPY*-DHFR also contain three hemagglutinin (HA)-tags at the C-terminus for detection with HA antibodies.

To analyze direct interactions of sCPY*-HA or sCPY*-DHFR-HA with potential recognition components, we employed a site-specific photocrosslinking method in intact yeast cells [23, 24]. Single amber stop codons were introduced at various positions of the polypeptide chain (Fig. 1A; positions upstream and downstream of the glycosylation site are given negative and positive numbers, respectively). These constructs were expressed together with a suppressor tRNA and a modified tRNA synthetase that charges the tRNA with a phenylalanine derivative containing a photoreactive benzophenone (Bpa). The photoreactive amino acid is then incorporated at the position specified by the amber codon. Irradiation of intact cells leads to crosslinks between the ERAD-L substrate and neighboring proteins (see scheme in Fig. 1B).

Figure 1
Experimental design of in vivo site-specific photocrosslinking experiments

We first tested interactions of sCPY*-HA and sCPY*-DHFR-HA with the soluble luminal domain of Hrd3p (1-767) fused to 13 Myc-tags, a construct that fully complements a HRD3 deletion [9]. After irradiation, cell extracts were subjected to immunoprecipitation with HA antibodies, followed by SDS-PAGE and immunoblotting with HA- or Myc- antibodies. The HA blot demonstrates that sCPY*-DHFR-HA is degraded less efficiently than the protein lacking the DHFR moiety, resulting in elevated steady-state levels. The Myc-blot shows that the most prominent crosslinks occurred with position +7 near the N-glycosylation site, although crosslinks were also observed with other positions. As expected, without irradiation, no crosslinks occurred ([9] and Supplementary Fig. S1A). When similar experiments were performed with a Hrd1p construct containing 13 Myc tags at its C-terminus, crosslinks were only observed with sCPY*-DHFR-HA, and not with sCPY* (Fig. 2D). As reported before [9], the crosslinks with sCPY*-DHFR-HA were observed primarily at positions +38 to +42. Given that the presence of the DHFR moiety increases the substrate population contacting Hrd1p but not that contacting Hrd3p, it appears that the rate-limiting step in the degradation of this substrate occurs after Hrd3p- function.

Figure 2
Photocrosslinking to Hrd3p

Consistent with the idea that substrate-Hrd3p interaction occurs early in ERAD-L, the crosslinking efficiency seen with sCPY* containing a probe at position +7 was not affected by deletion of ERAD components (Fig. 2B). Even the simultaneous deletion of several ERAD components had no effect (Fig. 2B). The glycosidase Htm1p was also not required, indicating that Hrd3p interaction is independent of an α1,6-mannose signal in the carbohydrate chain. Indeed, sCPY* lacking a N-glycosylation site crosslinked with the same efficiency as the glycosylated protein (Fig. 2C). Substrate interaction requires residues 1-392 of Hrd3p (Fig. S1B), consistent with previous suggestions [25]; the putative Yos9p-/Hrd1p-binding domain can be deleted without affecting crosslinking to substrate. Collectively, these data suggest that Hrd3p is involved in the early recognition of unstructured polypeptide segments. Such a chaperone-like function of Hrd3p is supported by the observation that Hrd3p interacts with many positions in sCPY* when substrate accumulates in the ER lumen. For example, positions that interacted only weakly with Hrd3p in wild type cells, crosslinked significantly stronger in ERAD-deletion mutants (Fig. 3A, B) or in a Hrd1p mutant with a defective RING finger domain (C399S) (Fig. S2). Thus, weaker binding sites for Hrd3p appear to exist throughout the unfolded polypeptide chain and become more prominent at elevated substrate levels.

Figure 3
Photocrosslinking to Hrd3p in different ERAD mutants

To investigate whether Yos9p contributes to polypeptide binding or is exclusively involved in sugar recognition, we performed similar crosslinking experiments in a strain expressing Yos9p containing 13 Myc-tags and an HDEL ER-retention signal at its C-terminus. As with Hrd3p, the tagged protein complements a deletion strain (data not shown). Using sCPY*-DHFR-HA, crosslinks were only observed with position −69 (Fig. 4A); no crosslinks were seen around the carbohydrate attachment site. The crosslinks at position −69 were significantly reduced when the substrate was non-glycosylated or when Hrd3p was absent (Fig. 4B). When both effects were combined, essentially no crosslinking to Yos9p was observed (Fig. 4C). Deletion of HTM1 had only a moderate effect on Yos9p crosslinking, while deletion of HRD1 did not affect it at all (Fig. 4B). The specificity for position −69 was not altered in a hrd1Δ strain (Supplementary Fig. S3). These results are consistent with the idea that Yos9p does not interact with the polypeptide segment around the carbohydrate attachment site that signals degradation. Rather, it appears that a distant segment comes in contact with Yos9p once Yos9p binds to the α1,6-signal in the carbohydrate chain. It should be noted that sCPY* without the attached DHFR domain did not give crosslinks at all (data not shown), consistent with the assumption that Yos9p acts downstream of Hrd3p.

Figure 4
Photocrosslinking of sCPY*-DHFR-HA to Yos9p

Finally, we studied the interaction of sCPY*-DHFR-HA with Der1p containing 13 myc-tags (Der1p-13myc), which retains partial activity [9]. Previous work has shown that position +24 of the substrate gives the most prominent crosslinks [9]. These crosslinks were still present in yos9Δ or hrd3Δ cells, but disappeared when USA1 was deleted (Fig. 5A). The interaction could be restored when fragments of Usa1p were expressed that are defective in either Usa1p-Hrd1p or Usa1p-Usa1p interaction (Fig. 5B). All constructs retain the C-terminal segment (residues 584-838) that has been proposed to interact with Der1p [11]. These data therefore support the idea that there are parallel pathways of substrate recruitment, one involving Hrd3p/Yos9p and another involving Der1p. In addition, it appears that Der1p does not need to be recruited to the Hrd1p complex to interact with substrate. The role of Usa1p may be in stabilizing Der1p, as the levels of Der1p are reduced in a usa1Δ strain [11].

Figure 5
Photocrosslinking to Der1p

4. Discussion

Our results suggest that there are two distinct pathways by which a misfolded luminal substrate is recognized for ERAD-L. The first pathway involves Hrd3p and Yos9p. Hrd3p appears to function upstream of Yos9p and interacts directly with the polypeptide substrate, including a segment around the carbohydrate attachment site. Yos9p may recognize exclusively the α1,6-mannose residue in the carbohydrate chain, as it crosslinks only far away from the degradation signal in the substrate, probably with segments that are irrelevant for actual recognition. The simultaneous interactions of Hrd3p with an unfolded polypeptide segment and of Yos9p with the α1,6-mannose signal would direct the complex of the two proteins to the region around the critical N-glycosylation site. This would explain why the degradation signal is bipartite, with neither an unfolded segment nor an α1,6-mannose signal alone being capable of directing a substrate to ERAD-L. Hrd3p may recognize many unfolded polypeptide segments, as it can crosslink throughout a polypeptide chain, particularly when the substrate is accumulated in the ER lumen. Hrd3p thus appears to be a general chaperone.

The second pathway of substrate recognition involves Der1p. Substrate delivery to Der1p does not require Yos9p or Hrd3p. It is not clear what feature in the substrate is recognized by Der1p, but it is interesting that Der1p interacts close to the degradation signal. Usa1p’s role in substrate interaction appears to be restricted to stabilizing Der1p. Both substrate delivery pathways subsequently converge at Hrd1p, which is the major component required for movement of the substrate across the membrane. Although Hrd3p is not required for substrate recognition in the Der1p pathway, it is involved in the transfer of substrate from Der1p to Hrd1p; substrate crosslinks to Hrd1p are abolished in the absence of Hrd3p or when Yos9p and Der1p are simultaneously absent [9]. Furthermore, because both Yos9p and Der1p are essential in ERAD-L, they must have non-redundant functions in addition to providing parallel pathways of substrate delivery to Hrd1p.

It is surprising that so many ERAD components are involved in substrate recognition. One reason may be the need to distinguish between ERAD substrates and folding intermediates. However, one would think that this could be achieved by the dual recognition of a processed carbohydrate signal (the terminal α1,6-mannose residue) and of an associated unfolded polypeptide segment by the Hrd3p/Yos9p complex. Perhaps, this type of recognition alone would not be specific enough, and it would not explain how non-glycosylated substrates are recognized. Der1p may serve as an additional recognition device, ensuring that only proper substrates are passed on to the central Hrd1p component.

Supplementary Material

01

Figure S1: UV-dependence of crosslinking to Hrd3p:

(A) sCPY*-HA with amber codons at the indicated positions were expressed together with Hrd3p-13myc and subjected to in vivophotocrosslinking. Membranes were isolated and SDS-denatured proteins were immunoprecipitated with HA antibodies. The samples were analyzed by SDS-PAGE and immunoblotting with either HA or Myc antibodies. Where indicated, UV irradiation was omitted. The arrow indicates the position of crosslinked bands. Some non-crosslinked Hrd-13myc is seen in the immunoprecipitations (asterisk). The open arrow indicates the position of substrate and of co-migrating immunoglobulin light chain. The double asterisk (**) denotes the position of immunoglobulin heavy chain. (B) sCPY*-HA with a photoreactive probe at position +7 was expressed together with Hrd3p-13myc comprising either the entire luminal domain of Hrd3 (residues 1-767) or residues 1-392.

02

Figure S2: Crosslinking to Hrd3p in a RING finger mutant of Hrd1p:

sCPY*-HA with amber codons at the indicated positions were expressed together with Hrd3p-13myc in hrd1Δ cells containing a plasmid coding for either wild type (WT) Hrd1p or a Hrd1p mutant with the C399S mutation in the RING finger domain. Cells were UV-irradiated as indicated, membranes were isolated, and SDS-denatured proteins were immunoprecipitated with HA antibodies. The samples were analyzed by SDS-PAGE and immunoblotting with either HA or Myc antibodies. The prominent UV-independent band in the Myc blot is non-specifically precipitated non-crosslinked Hrd3-13myc. The prominent UV-dependent band seen with some positions in the HA blot corresponds to crosslinkedsCPY*-HA dimers.

03

Figure S3: Photocrosslinking to Yos9p in a HRD1 deletion strain:

sCPY*-HA with amber codons at the indicated positions were expressed together with Yos9p-13myc in cells lacking HRD1. After in vivophotocrosslinking, membranes were isolated and SDS-denatured proteins were immunoprecipitated with HA antibodies. The samples were analyzed by SDS-PAGE and immunoblotting with either HA or Myc antibodies.

Acknowledgments

We thank P. Schultz and J. Weissman for reagents. A.M.S. was supported by a Ruth L. Kirschstein National Research Service Award postdoctoral fellowship. P.C. is supported by a Leukemia and Lymphoma Society special fellow award. T.A.R. is supported by NIH grant GM052586 and is a Howard Hughes Medical Institute Investigator.

Abbreviations

CPY
carboxypeptidase Y
DHFR
dihydrofolate reductase
ER
endoplasmic reticulum
ERAD
endoplasmic reticulum-associated degradation
HA
hemagglutinin
sCPY
short carboxypeptidase Y

Footnotes

Structured summary of protein interactions:

sCPY physically interacts with sCPY by cross-linking study (View interaction)

Hrd3 and sCPY physically interact by cross-linking study (View interaction)

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