Single fiber electromyography (SFEMG) was established by Stalberg and Eskedt in the 1960s, and is of proven value in the diagnosis of neuromuscular disorders, especially myasthenia gravis. It has proved to be the most sensitive technique in detecting a neuromuscular transmission defect in comparison with the tensilon test, repetitive stimulation, and acetyl choline receptor antibody estimation.[2,3]
Single fiber electromyography typically requires the use of a specially contracted single fiber EMG needle electrode[Figure 1] or facial concentric needle electrode [Figure 2] with a small recording surface (25 micrometers), which is exposed at a port on the side of the electrode, 3 mm from the tip.
The validity of the technique has been proven by examining a large number of myasthenia patients with a sensitivity of up to 99% in detecting a neuromuscular transmission defect. in generalized myasthenia gravis has been reported.[2,4] When a motor axon is depolarized the action potentials travel distally and excite the muscle fiber more or less at the same time. The variation in the time interval between the two action potentials of the same motor unit is called as “jitter”. SFEMG measures the variation of this inter potential interval (jitter)[Figure 3].
Most of the modern nerve conduction and EMG machines have a software to perform and analyze the SFEMG examination. There are two methods to perform this. One is stimulated and the other is under a volitional effort. However, most physicians, including the author, prefer to perform SFEMG under the volitional effort. The goal of SFEMG is to study the adjacent action potentials from the same motor unit, known as ‘pairs’. This is achieved by using a specially constructed single fiber needle electrode or facial concentric needle electrode. This identifies and selectively records the action potentials from individual muscle fibers. The selectivity of the recording is further strengthened by adjusting the filter settings. (Low frequencies filter 500 HZ – high frequencies filter 10 KHZ). This filter setting selectively abolishes low frequency components from distant muscle fibers. Action potentials should be greater than 200 mictovolts in amplitude and the rise time should be less than 300 microseconds. Around 20 potential pairs are collected from the same muscle by three-to-four insertions. The subject is asked to maintain a steady contraction if volitional SFEMG is undertaken, until 100 consecutive discharges are recorded from each pair.
Stimulation SFEMG is particularly useful in children, uncooperative, comatose patients, and those who have tremors. A branch of the motor nerve is stimulated by using a mono polar needle electrode and recording is made by SFEMG or a concentric needle electrode. Stimulation is delivered at 2 - 10 HZ and the stimulus intensity is adjusted accordingly.
Jitter is the measurement of variation of the inter-potential interval. This is calculated between the triggered potential and the time-locked, second single muscle fiber action potential [Figures [Figures44–7]. This is expressed as a mean consecutive difference (MCD). Most modern EMG machines have a program that automatically performs the MCD calculation. Mean MCD is calculated using the following formula:
When neuromuscular transmission is sufficiently impaired, nerve impulses fail to elicit an action potential and this is called ‘blocking’ [Figure 8]. This usually happens when the jitter value is markedly prolonged, usually when MCD is more than 100 microseconds.
The normal jitter values have been determined for many muscles in a multicenter collaborative study[6,7][Figure 9].
The study is considered abnormal if one of the following criteria is met,
- Mean jitter value exceeds the upper limit of the normal value
- More than 10% of the pairs have increased jitter (two out of twenty pairs)
Normal values apply only if the inter-spike interval is up to four microseconds. Errors may be encountered if this is higher and may produce a false jitter.