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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Int J Gynaecol Obstet. Author manuscript; available in PMC Jun 3, 2011.
Published in final edited form as:
PMCID: PMC3107851
NIHMSID: NIHMS293625
Effect of estradiol on oocyte development
Katherine Bianco,a* Neal G. Mahutte,b Aydin Arici,b Denny Sakkas,b and Hugh S. Taylorb
aDepartment of Obstetrics, Gynecology and Reproductive Sciences, University of California, San Francisco, California, USA
bDepartment of Obstetrics, Gynecology and Reproductive Sciences, Yale University School of Medicine, New Haven, Connecticut, USA
* Corresponding author. Department of Obstetrics and Gynecology, University of, California, San Francisco, 505 Parnassus Avenue, Box 0132, San Francisco, CA 94143, USA. Tel.: +1 415 443 4031., biancok/at/obgyn.ucsf.edu (K. Bianco)
Objective
To determine whether elevated serum estradiol (E2) concentrations in oocyte donors affect assisted reproduction outcome.
Method
In a retrospective cohort study of 58 consecutive oocyte donation cycles, donors were stratified into 2 groups according to E2 concentration, group 1 (n=32; E2≤2000 pg/mL [range 456–2000 pg/mL]) and group 2 (n=27; E2>2000 pg/mL [range, 2062–6957 pg/mL]). Data were analyzed using the t test and χ2 test.
Results
Donors in group 1 produced significantly less oocytes than donors in group 2 (19.3±1.7 vs 12.0±1.4; P<0.001), and recipients of oocytes from group 1 had significantly fewer numbers of embryos available for transfer (10.4±1.1 vs 6.4±0.8; P=0.003). However, the mean number (3.3) of embryos transferred and the pregnancy rate were the same in both groups.
Conclusion
Elevated estradiol concentration in oocyte donors did not affect pregnancy outcome, suggesting that estradiol levels in donors do not affect oocyte development.
Keywords: Oocyte donation, Estradiol (E2), In vitro fertilization, Oocyte biology
Assisted reproductive technologies (ART) aim at stimulating the ovaries to produce a large number of oocytes and maximize the chances of pregnancy. The effect of supraphysiologic estradiol (E2) concentration on ART outcome is controversial [16]. Recently, it has been suggested that elevated estradiol concentrations may have adverse effects, not only on the endometrium but also on the oocyte and/or embryo [5,6]. More generally, the success of oocyte donation is influenced by multiple factors such as donor age, oocyte and embryo quality, and the recipient's endometrial receptivity [79].
In oocyte donation, the donor's gametogenesis and ovarian steroidogenesis are dissociated from the recipient's endometrial development and receptivity, allowing the effects of elevated estradiol concentrations on the embryo to be distinguished from those on the endometrium. One study of oocyte donation cycles that examined the effects of elevated E2 peak concentrations in donors found a detrimental effect on endometrial receptivity; however, the lack of donor uniformity was certainly a limitation, with recipients receiving supernumerary oocytes from infertile donors who themselves were undergoing in vitro fertilization and embryo transfer [5]. Other studies, as well as the present study, used a protocol where each recipient received all the oocytes retrieved from a designated donor, and oocytes from infertile women were not included [1].
To differentiate the effects of elevated estradiol concentration in the donor on the oocyte from the effects of elevated estradiol concentration in the recipient on the latter's endometrium in oocyte donation cycles, we examined the relationships between ART outcome and donor estradiol concentration, quantity of oocytes, and quantity of embryos.
We conducted a retrospective cohort study of 58 consecutive oocyte donation cycles from January 2000 to December 2002 within the framework of the Yale University Oocyte Donation and Surrogacy Program. Specifically, we examined the relationship between cycle characteristics and ART outcomes. Inclusion criteria for oocyte donors were age less than 35 years; serum levels of follicle stimulating hormone less than 10 IU/mL and serum levels of E2 less than 50 pg/mL on day 3 of the cycle; no history of infertility; and no significant ongoing medical problems. None of the men had severe oliogspermia (sperm count <10 ×1 0−6/mL). All transfers were made on day 3 of the cycle with embryos of more than 7 cells. The donors were stratified into 2 groups based on their peak estradiol level, group 1 (n=32; E2≤2000 pg/mL [range 456–2000 pg/mL]) and group 2 (n=27; E2>2000 pg/mL [range, 2062–6957 pg/mL]). Oocytes from infertile women were excluded. The outcomes examined in each group were number of oocytes, number of embryos, clinical pregnancy rates, and live birth rates.
A standard synchronization regimen was used for oocyte donor and recipient, as previously described [1012]. Each recipient underwent a mock cycle followed with an endometrial biopsy, and all the oocytes retrieved from a designated donor were available to her. Recipients with gonadal function first underwent pituitary downregulation with leuprolide acetate. In all cases, recipient and donor were synchronized by taking oral micronized E2 approximately 5 days before the donor was administered human chorionic gonadotrophin (hCG) to provoke ovulation. The recipient started taking progesterone twice daily on the morning of the day before the donor underwent oocyte retrieval, and continued the treatment thereafter. All embryos were transferred 72 hours following oocyte retrieval. Peak donor E2 serum levels were measured on the morning of hCG administration. Before transfer, the embryos were graded from 1 to 5 according to established criteria [13].
A serum pregnancy test was done 12 to 14 days after treatment, but clinical pregnancy was confirmed by the presence of a gestational sac on ultrasound. Chemical pregnancy was defined as the presence of the beta subunit of hCG without any evidence of a gestational sac. Abortion was defined as a pregnancy loss prior to 20 weeks of gestation, with fetal heartbeat previously documented. Serum levels of E2 were measured by chemiluminescent enzyme immunoassays (Immulite; Diagnostic Products, Los Angeles, CA, USA). Intra-assay and interassay coefficients of variation did not exceed 9.3% and 10.5%, respectively. The protocol was approved by the Yale University School of Medicine institutional review board. Statistical analyses were performed using the t test or the χ2 test, as indicated. P<0.05 was considered significant.
The relevant baseline characteristics of the 2 groups are outlined in Table 1. As expected, the donors' population was younger than the recipients' (27.9 years [range, 21–34 years] vs 42.5 years [range, 31– 55 years]). The percentage of recipients undergoing intracytoplasmic sperm injection was 24.4%.
Table 1
Table 1
Relevant characteristics of donors and recipients a
Significantly more oocytes were retrieved from donors with E2 levels higher than 2000 pg/mL than from donors with E2 values of 2000 pg/mL or less (19.3±1.7 vs 12.0±1.4; P<0.001). Similarly, more embryos were produced with oocytes from donors with peak E2 levels higher than 2000 pg/mL than those with E2 values of 2000 pg/mL or less (10.4±1.1 vs 6.4±0.8; P<0.001) (Table 2).
Table 2
Table 2
Assisted reproduction outcomes according to donor E2 level a
However, the mean number of embryos transferred in each group was the same, 3.3, and all embryos comprised more than 7 cells on day 3, with no differences in embryo quality (ie, cell number or grade). Moreover, there were no differences in clinical pregnancy rates (58.1% [18/31] vs 59.2% [16/27]; P=0.93) or live birth rates (54.8% [17/31] vs. 55.6% [15/27]; P=0.95) between the 2 groups (Table 2). The study had more than 80% power to detect a difference in pregnancy rates greater than 15%.
There were 6 chemical pregnancies, 3 spontaneous abortions, and 28 clinical pregnancies for which we were able to obtain complete obstetric outcomes. The mean±SD gestational age at delivery was 36.7±3.1; 53.5% of the pregnancies were multiple; 25% were spontaneous vaginal deliveries and 75% were cesarean deliveries. No differences in gestational age at delivery or mode of delivery were found between the 2 groups.
The success of oocyte donation is influenced by multiple factors, including donor age, embryo quality, and the recipient's endometrial receptivity. Elevated E2 levels have been thought to inhibit implantation in animals and humans [1416]. Oocytes (and thus, the embryos) obtained from young donors may have superior potential compared with oocytes obtained from patients with impaired fertility who are undergoing in vitro fertilization and embryo transfer [2,8]. It has been postulated that increased implantation rates in oocyte donation resulted from a more physiologic hormonal milieu that uncoupled endometrial receptivity from oocyte production [16]. Previous reports comparing natural cycles with ovarian stimulation cycles found a higher incidence of dys-synchrony between endometrial glands and stroma in the ovarian stimulation cycles [17]. There is evidence of a significant reduction in nuclear receptors for progesterone and estrogen in both gland and stroma after ovarian stimulation. Implantation could be affected if these endometrial responses to ovarian stimulation changed the endometrial morphology and affected the window of receptivity [6,18,19]. In contrast, a large amount of evidence suggests that high levels of estradiol are not detrimental to oocyte quality, fertilization, and embryo cleavage [1,3,8,2022].
To discern the impact of estradiol levels on folliculogenesis and endometrial receptivity is difficult in conventional in vitro fertilization cycles. However, the oocyte donation model allows to study isolated parameters that may affect outcome, and compare their effects on embryo quality and endometrium receptivity [23].
In the present study, the donors were typically young, healthy, not infertile—a more homogeneous cohort than infertile populations. Similarly, endometrial receptivity was relatively constant in the recipients because the uniform artificial preparation, as confirmed by endometrial biopsy in a mock cycle.
As expected, our study demonstrated that greater numbers of oocytes, and therefore greater numbers of embryos, were obtained when donors had higher levels of estradiol. Other studies also indicate that high peak E2 levels are not detrimental to oocyte quality, fertilization, and embryo cleavage; and that to the contrary, elevated E2 levels result in a greater number of oocytes and embryos for selection at the time of embryo transfer or cryopreservation [1,3,14,20,21]. These findings are in agreement with observations for high responder (those with peak E2>2000) undergoing conventional in vitro fertilization. The lesser embryo implantation rates must therefore be imputed to an endometrial effect rather than to egg quality [3,14,2022].
In conclusion, elevated E2 levels in donors were not found to affect pregnancy outcome in oocyte donation cycles. This suggests elevated E2 levels do not compromise oocyte quality or embryo development in vitro, but that elevated E2 levels may diminish endometrial receptivity.
1. Pena JE, Chang PL, Thornton MH, II, Sauer MV. Serum estradiol levels after 4 days of ovarian hyperstimulation in oocyte donors are predictive of embryo quality and clinical outcomes. Gynecol Obstet Invest. 2002;54(4):207–212. [PubMed]
2. Levi AJ, Drews MR, Bergh PA, Miller BT, Scott RT., Jr Controlled ovarian hyperstimulation does not adversely affect endometrial receptivity in in vitro fertilization cycles. Fertil Steril. 2001;76(4):670–674. [PubMed]
3. Ng EH. What is the threshold value for serum estradiol levels associated with adverse IVF outcomes? Fertil Steril. 2000;73(5):1071–1072. [PubMed]
4. Sharara FI, McClamrock HD. High estradiol levels and high oocyte yield are not detrimental to in vitro fertilization outcome. Fertil Steril. 1999;72(3):401–405. [PubMed]
5. Simon C, Cano F, Valbuena D, Remohi J, Pellicer A. Clinical evidence for a detrimental effect on uterine receptivity of high serum oestradiol concentrations in high and normal responder patients. Hum Reprod. 1995;10(9):2432–2437. [PubMed]
6. Valbuena D, Martin J, de Pablo JL, Remohi J, Pellicer A, Simon C. Increasing levels of estradiol are deleterious to embryonic implantation because they directly affect the embryo. Fertil Steril. 2001;76(5):962–968. [PubMed]
7. Stolwijk AM, Zielhuis GA, Sauer MV, Hamilton CJ, Paulson RJ. The impact of the woman's age on the success of standard and donor in vitro fertilization. Fertil Steril. 1997;67(4):702–710. [PubMed]
8. Noyes N, Hampton BS, Berkeley A, Licciardi F, Grifo J, Krey L. Factors useful in predicting the success of oocyte donation: a 3-year retrospective analysis. Fertil Steril. 2001;76(1):92–97. [PubMed]
9. Taylor HS. The role of HOX genes in the development and function of the female reproductive tract. Semin Reprod Med. 2000;18(1):81–89. [PubMed]
10. De Ziegler D, Fanchin R, Massonneau M, Bergeron C, Frydman R, Bouchard P. Hormonal control of endometrial receptivity: the egg donation model and controlled ovarian hyperstimulation. Ann N Y Acad Sci. 1994;734:209–220. [PubMed]
11. Devroey P, Pados G. Preparation of endometrium for egg donation. Hum Reprod Update. 1998;4(6):856–861. [PubMed]
12. Marcus SF, Brinsden PR. In-vitro fertilization and embryo transfer in women aged 40 years and over. Hum Reprod Update. 1996;2(6):459–468. [PubMed]
13. Veek LL. Atlas of Human Oocyte and Early Conceptus. Baltimore, Maryland, USA: Williams & Wilkin; 1991.
14. Fossum GT, Davidson A, Paulson RJ. Ovarian hyperstimulation inhibits embryo implantation in the mouse. J In Vitro Fert Embryo Transf. 1989;6(1):7–10. [PubMed]
15. Morris JM, Van Wagenen G. Interception: the use of postovulatory estrogens to prevent implantation. Am J Obstet Gynecol. 1973;115(1):101–106. [PubMed]
16. Paulson RJ, Sauer MV, Lobo RA. Embryo implantation after human in vitro fertilization: importance of endometrial receptivity. Fertil Steril. 1990;53(5):870–874. [PubMed]
17. Benadiva CA, Metzger DA. Superovulation with human menopausal gonadotropins is associated with endometrial gland-stroma dyssynchrony. Fertil Steril. 1994;61(4):700–704. [PubMed]
18. Horcajadas JA, Riesewijk A, Polman J, van Os R, Pellicer A, Mosselman S, et al. Effect of controlled ovarian hyperstimulation in IVF on endometrial gene expression profiles. Mol Hum Reprod. 2005;11(5):195–205. [PubMed]
19. Develioglu OH, Hsiu JG, Nikas G, Toner JP, Oehninger S, Jones HW. Endometrial estrogen and progesterone receptor and pinopod expression in stimulated cycles in oocyte donation. Fertil Steril. 1999;71(6):1040–1047. [PubMed]
20. Chenette PE, Sauer MV, Paulson RJ. Very high serum estradiol levels are not detrimental to clinical outcome of in vitro fertilization. Fertil Steril. 1990;54(5):858–863. [PubMed]
21. Jain A, Robins JC, Williams DB, Thomas MA. The effect of multiple cycles in oocyte donors. Am J Obstet Gynecol. 2005;192(5):1382–1384. [PubMed]
22. Pena JE, Chang PL, Chan LK, Zeitoun K, Thornton MH, II, Sauer MV. Supraphysiological estradiol levels do not affect oocyte and embryo quality in oocyte donation cycles. Hum Reprod. 2002;17(1):83–87. [PubMed]
23. Lindheim SR, Morales AJ. GnRH antagonists followed by a decline in serum estradiol results in adverse outcomes in donor oocyte cycles. Hum Reprod. 2003;18(10):2048–2051. [PubMed]