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Nucleic Acids Res. 1993 November 25; 21(23): 5345–5350.
PMCID: PMC310569

Structure of the gene encoding the 14.5 kDa subunit of human RNA polymerase II.

Abstract

The structure of the gene encoding the 14.5 kDa subunit of the human RNA polymerase II (or B) has been elucidated. The gene consists of six exons, ranging from 52 to over 101 bp, interspaced with five introns ranging from 84 to 246 bp. It is transcribed into three major RNA species, present at low abundance in exponentially growing HeLa cells. The corresponding messenger RNAs contain the same open reading frame encoding a 125 amino acid residue protein, with a calculated molecular weight of 14,523 Da. This protein (named hRPB14.5) shares strong homologies with the homologous polymerase subunits encoded by the Drosophila (RpII15) and yeast (RPB9) genes. Cysteines characteristic of two zinc fingers are conserved in all three corresponding sequences and, like the yeast protein, the hRPB14.5 subunit exhibits zinc-binding activity.

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Selected References

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  • Sawadogo M, Sentenac A. RNA polymerase B (II) and general transcription factors. Annu Rev Biochem. 1990;59:711–754. [PubMed]
  • Zawel L, Reinberg D. Advances in RNA polymerase II transcription. Curr Opin Cell Biol. 1992 Jun;4(3):488–495. [PubMed]
  • Ham J, Steger G, Yaniv M. How do eukaryotic activator proteins stimulate the rate of transcription by RNA polymerase II? FEBS Lett. 1992 Jul 27;307(1):81–86. [PubMed]
  • Sentenac A. Eukaryotic RNA polymerases. CRC Crit Rev Biochem. 1985;18(1):31–90. [PubMed]
  • Young RA. RNA polymerase II. Annu Rev Biochem. 1991;60:689–715. [PubMed]
  • Manley JL, Fire A, Cano A, Sharp PA, Gefter ML. DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3855–3859. [PubMed]
  • Dignam JD, Lebovitz RM, Roeder RG. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res. 1983 Mar 11;11(5):1475–1489. [PMC free article] [PubMed]
  • Wintzerith M, Acker J, Vicaire S, Vigneron M, Kedinger C. Complete sequence of the human RNA polymerase II largest subunit. Nucleic Acids Res. 1992 Feb 25;20(4):910–910. [PMC free article] [PubMed]
  • Ahearn JM, Jr, Bartolomei MS, West ML, Cisek LJ, Corden JL. Cloning and sequence analysis of the mouse genomic locus encoding the largest subunit of RNA polymerase II. J Biol Chem. 1987 Aug 5;262(22):10695–10705. [PubMed]
  • Jokerst RS, Weeks JR, Zehring WA, Greenleaf AL. Analysis of the gene encoding the largest subunit of RNA polymerase II in Drosophila. Mol Gen Genet. 1989 Jan;215(2):266–275. [PubMed]
  • Bird DM, Riddle DL. Molecular cloning and sequencing of ama-1, the gene encoding the largest subunit of Caenorhabditis elegans RNA polymerase II. Mol Cell Biol. 1989 Oct;9(10):4119–4130. [PMC free article] [PubMed]
  • Smith JL, Levin JR, Ingles CJ, Agabian N. In trypanosomes the homolog of the largest subunit of RNA polymerase II is encoded by two genes and has a highly unusual C-terminal domain structure. Cell. 1989 Mar 10;56(5):815–827. [PubMed]
  • Allison LA, Moyle M, Shales M, Ingles CJ. Extensive homology among the largest subunits of eukaryotic and prokaryotic RNA polymerases. Cell. 1985 Sep;42(2):599–610. [PubMed]
  • Leffers H, Gropp F, Lottspeich F, Zillig W, Garrett RA. Sequence, organization, transcription and evolution of RNA polymerase subunit genes from the archaebacterial extreme halophiles Halobacterium halobium and Halococcus morrhuae. J Mol Biol. 1989 Mar 5;206(1):1–17. [PubMed]
  • Pühler G, Leffers H, Gropp F, Palm P, Klenk HP, Lottspeich F, Garrett RA, Zillig W. Archaebacterial DNA-dependent RNA polymerases testify to the evolution of the eukaryotic nuclear genome. Proc Natl Acad Sci U S A. 1989 Jun;86(12):4569–4573. [PubMed]
  • Ovchinnikov YuA, Monastyrskaya GS, Gubanov VV, Guryev SO, Salomatina IS, Shuvaeva TM, Lipkin VM, Sverdlov ED. The primary structure of E. coli RNA polymerase, Nucleotide sequence of the rpoC gene and amino acid sequence of the beta'-subunit. Nucleic Acids Res. 1982 Jul 10;10(13):4035–4044. [PMC free article] [PubMed]
  • Acker J, Wintzerith M, Vigneron M, Kédinger C. Primary structure of the second largest subunit of human RNA polymerase II (or B). J Mol Biol. 1992 Aug 20;226(4):1295–1299. [PubMed]
  • Falkenburg D, Dworniczak B, Faust DM, Bautz EK. RNA polymerase II of Drosophila. Relation of its 140,000 Mr subunit to the beta subunit of Escherichia coli RNA polymerase. J Mol Biol. 1987 Jun 20;195(4):929–937. [PubMed]
  • Sweetser D, Nonet M, Young RA. Prokaryotic and eukaryotic RNA polymerases have homologous core subunits. Proc Natl Acad Sci U S A. 1987 Mar;84(5):1192–1196. [PubMed]
  • Lisitsyn NA, Monastyrskaya GS, Sverdlov ED. Genes coding for RNA polymerase beta subunit in bacteria. Structure/function analysis. Eur J Biochem. 1988 Nov 1;177(2):363–369. [PubMed]
  • Kolodziej P, Young RA. RNA polymerase II subunit RPB3 is an essential component of the mRNA transcription apparatus. Mol Cell Biol. 1989 Dec;9(12):5387–5394. [PMC free article] [PubMed]
  • Woychik NA, Young RA. RNA polymerase II subunit RPB4 is essential for high- and low-temperature yeast cell growth. Mol Cell Biol. 1989 Jul;9(7):2854–2859. [PMC free article] [PubMed]
  • Woychik NA, Liao SM, Kolodziej PA, Young RA. Subunits shared by eukaryotic nuclear RNA polymerases. Genes Dev. 1990 Mar;4(3):313–323. [PubMed]
  • McKune K, Richards KL, Edwards AM, Young RA, Woychik NA. RPB7, one of two dissociable subunits of yeast RNA polymerase II, is essential for cell viability. Yeast. 1993 Mar;9(3):295–299. [PubMed]
  • Woychik NA, Lane WS, Young RA. Yeast RNA polymerase II subunit RPB9 is essential for growth at temperature extremes. J Biol Chem. 1991 Oct 5;266(28):19053–19055. [PubMed]
  • Treich I, Carles C, Riva M, Sentenac A. RPC10 encodes a new mini subunit shared by yeast nuclear RNA polymerases. Gene Expr. 1992;2(1):31–37. [PubMed]
  • Woychik NA, Young RA. RNA polymerase II subunit RPB10 is essential for yeast cell viability. J Biol Chem. 1990 Oct 15;265(29):17816–17819. [PubMed]
  • Woychik NA, McKune K, Lane WS, Young RA. Yeast RNA polymerase II subunit RPB11 is related to a subunit shared by RNA polymerase I and III. Gene Expr. 1993;3(1):77–82. [PubMed]
  • Carles C, Treich I, Bouet F, Riva M, Sentenac A. Two additional common subunits, ABC10 alpha and ABC10 beta, are shared by yeast RNA polymerases. J Biol Chem. 1991 Dec 15;266(35):24092–24096. [PubMed]
  • Pati UK, Weissman SM. Isolation and molecular characterization of a cDNA encoding the 23-kDa subunit of human RNA polymerase II. J Biol Chem. 1989 Aug 5;264(22):13114–13121. [PubMed]
  • Pati UK, Weissman SM. The amino acid sequence of the human RNA polymerase II 33-kDa subunit hRPB 33 is highly conserved among eukaryotes. J Biol Chem. 1990 May 25;265(15):8400–8403. [PubMed]
  • Harrison DA, Mortin MA, Corces VG. The RNA polymerase II 15-kilodalton subunit is essential for viability in Drosophila melanogaster. Mol Cell Biol. 1992 Mar;12(3):928–935. [PMC free article] [PubMed]
  • Zajchowski DA, Boeuf H, Kédinger C. The adenovirus-2 early EIIa transcription unit possesses two overlapping promoters with different sequence requirements for EIa-dependent stimulation. EMBO J. 1985 May;4(5):1293–1300. [PubMed]
  • Zajchowski DA, Boeuf H, Kédinger C. E1a inducibility of the adenoviral early E2a promoter is determined by specific combinations of sequence elements. Gene. 1987;58(2-3):243–256. [PubMed]
  • Studier FW, Rosenberg AH, Dunn JJ, Dubendorff JW. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 1990;185:60–89. [PubMed]
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. [PubMed]
  • Mazen A, Gradwohl G, de Murcia G. Zinc-binding proteins detected by protein blotting. Anal Biochem. 1988 Jul;172(1):39–42. [PubMed]
  • Higgins DG, Sharp PM. CLUSTAL: a package for performing multiple sequence alignment on a microcomputer. Gene. 1988 Dec 15;73(1):237–244. [PubMed]
  • Treich I, Riva M, Sentenac A. Zinc-binding subunits of yeast RNA polymerases. J Biol Chem. 1991 Nov 15;266(32):21971–21976. [PubMed]
  • Kozak M. A consideration of alternative models for the initiation of translation in eukaryotes. Crit Rev Biochem Mol Biol. 1992;27(4-5):385–402. [PubMed]
  • Smale ST, Baltimore D. The "initiator" as a transcription control element. Cell. 1989 Apr 7;57(1):103–113. [PubMed]

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