PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of narLink to Publisher's site
 
Nucleic Acids Res. 1992 January 25; 20(2): 295–301.
PMCID: PMC310369

A new rRNA processing mutant of Saccharomyces cerevisiae.

Abstract

We have identified from a collection of temperature sensitive yeast mutants strains which fail to process rRNA normally. Characterization of one such mutant is reported here. This strain accumulates increased amounts of the 35S primary transcript, '24S' molecules extending from the transcription start site to the 5.8S region, and two classes of 5.8S rRNA with 5' extensions of 7 and 149 bases, respectively. We show that this pleiotropic change in the rRNA processing pattern is due to a single mutation. Possible models for the function of the mutated gene are discussed.

Full text

Full text is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.5M), or click on a page image below to browse page by page. Links to PubMed are also available for Selected References.

Selected References

These references are in PubMed. This may not be the complete list of references from this article.
  • Warner JR. Synthesis of ribosomes in Saccharomyces cerevisiae. Microbiol Rev. 1989 Jun;53(2):256–271. [PMC free article] [PubMed]
  • Raué HA, Planta RJ. Ribosome biogenesis in yeast. Prog Nucleic Acid Res Mol Biol. 1991;41:89–129. [PubMed]
  • Udem SA, Warner JR. Ribosomal RNA synthesis in Saccharomyces cerevisiae. J Mol Biol. 1972 Mar 28;65(2):227–242. [PubMed]
  • Kass S, Tyc K, Steitz JA, Sollner-Webb B. The U3 small nucleolar ribonucleoprotein functions in the first step of preribosomal RNA processing. Cell. 1990 Mar 23;60(6):897–908. [PubMed]
  • Savino R, Gerbi SA. In vivo disruption of Xenopus U3 snRNA affects ribosomal RNA processing. EMBO J. 1990 Jul;9(7):2299–2308. [PubMed]
  • Tollervey D. A yeast small nuclear RNA is required for normal processing of pre-ribosomal RNA. EMBO J. 1987 Dec 20;6(13):4169–4175. [PubMed]
  • Jarmolowski A, Zagorski J, Li HV, Fournier MJ. Identification of essential elements in U14 RNA of Saccharomyces cerevisiae. EMBO J. 1990 Dec;9(13):4503–4509. [PubMed]
  • Li HD, Zagorski J, Fournier MJ. Depletion of U14 small nuclear RNA (snR128) disrupts production of 18S rRNA in Saccharomyces cerevisiae. Mol Cell Biol. 1990 Mar;10(3):1145–1152. [PMC free article] [PubMed]
  • Tollervey D, Lehtonen H, Carmo-Fonseca M, Hurt EC. The small nucleolar RNP protein NOP1 (fibrillarin) is required for pre-rRNA processing in yeast. EMBO J. 1991 Mar;10(3):573–583. [PubMed]
  • Finley D, Bartel B, Varshavsky A. The tails of ubiquitin precursors are ribosomal proteins whose fusion to ubiquitin facilitates ribosome biogenesis. Nature. 1989 Mar 30;338(6214):394–401. [PubMed]
  • Sachs AB, Davis RW. Translation initiation and ribosomal biogenesis: involvement of a putative rRNA helicase and RPL46. Science. 1990 Mar 2;247(4946):1077–1079. [PubMed]
  • Vijayraghavan U, Company M, Abelson J. Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes Dev. 1989 Aug;3(8):1206–1216. [PubMed]
  • Denhardt DT. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. [PubMed]
  • Hurley DL, Angerer LM, Angerer RC. Altered expression of spatially regulated embryonic genes in the progeny of separated sea urchin blastomeres. Development. 1989 Jul;106(3):567–579. [PubMed]
  • Veldman GM, Brand RC, Klootwijk J, Planta R. Some characteristics of processing sites in ribosomal precursor RNA of yeast. Nucleic Acids Res. 1980 Jul 11;8(13):2907–2920. [PMC free article] [PubMed]
  • Veldman GM, Klootwijk J, van Heerikhuizen H, Planta RJ. The nucleotide sequence of the intergenic region between the 5.8S and 26S rRNA genes of the yeast ribosomal RNA operon. Possible implications for the interaction between 5.8S and 26S rRNA and the processing of the primary transcript. Nucleic Acids Res. 1981 Oct 10;9(19):4847–4862. [PMC free article] [PubMed]
  • Klootwijk J, Verbeet MP, Veldman GM, de Regt VC, van Heerikhuizen H, Bogerd J, Planta RJ. The in vivo and in vitro initiation site for transcription of the rRNA operon of Saccharomyces carlsbergensis. Nucleic Acids Res. 1984 Feb 10;12(3):1377–1390. [PMC free article] [PubMed]
  • Rubin GM. The nucleotide sequence of Saccharomyces cerevisiae 5.8 S ribosomal ribonucleic acid. J Biol Chem. 1973 Jun 10;248(11):3860–3875. [PubMed]
  • McCormick JR, Zengel JM, Lindahl L. Intermediates in the degradation of mRNA from the lactose operon of Escherichia coli. Nucleic Acids Res. 1991 May 25;19(10):2767–2776. [PMC free article] [PubMed]
  • Shuai K, Warner JR. A temperature sensitive mutant of Saccharomyces cerevisiae defective in pre-rRNA processing. Nucleic Acids Res. 1991 Sep 25;19(18):5059–5064. [PMC free article] [PubMed]
  • De Jonge P, Kastelein RA, Planta RJ. Non-ribosomal nucleotide sequences in 7-S RNA, the immediate precursor of 5.8-S ribosomal RNA in yeast. Eur J Biochem. 1978 Feb;83(2):537–546. [PubMed]
  • Rubin GM. Three forms of the 5.8-S ribosomal RNA species in Saccharomyces cerevisiae. Eur J Biochem. 1974 Jan 3;41(1):197–202. [PubMed]
  • Ford PJ, Mathieson T. The nucleotide sequences of 5.8-S ribosomal RNA from Xenopus laevis and Xenopus borealis. Eur J Biochem. 1978 Jun 1;87(1):199–214. [PubMed]
  • Bowman LH, Goldman WE, Goldberg GI, Hebert MB, Schlessinger D. Location of the initial cleavage sites in mouse pre-rRNA. Mol Cell Biol. 1983 Aug;3(8):1501–1510. [PMC free article] [PubMed]
  • Smith SD, Banerjee N, Sitz TO. Gene heterogeneity: a basis for alternative 5.8S rRNA processing. Biochemistry. 1984 Jul 31;23(16):3648–3652. [PubMed]
  • Srivastava AK, Schlessinger D. Mechanism and regulation of bacterial ribosomal RNA processing. Annu Rev Microbiol. 1990;44:105–129. [PubMed]
  • King TC, Sirdeshmukh R, Schlessinger D. RNase III cleavage is obligate for maturation but not for function of Escherichia coli pre-23S rRNA. Proc Natl Acad Sci U S A. 1984 Jan;81(1):185–188. [PubMed]
  • Maden BE, Dent CL, Farrell TE, Garde J, McCallum FS, Wakeman JA. Clones of human ribosomal DNA containing the complete 18 S-rRNA and 28 S-rRNA genes. Characterization, a detailed map of the human ribosomal transcription unit and diversity among clones. Biochem J. 1987 Sep 1;246(2):519–527. [PubMed]
  • Gonzalez IL, Chambers C, Gorski JL, Stambolian D, Schmickel RD, Sylvester JE. Sequence and structure correlation of human ribosomal transcribed spacers. J Mol Biol. 1990 Mar 5;212(1):27–35. [PubMed]
  • Smolik-Utlaut S, Petes TD. Recombination of plasmids into the Saccharomyces cerevisiae chromosome is reduced by small amounts of sequence heterogeneity. Mol Cell Biol. 1983 Jul;3(7):1204–1211. [PMC free article] [PubMed]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press