According to the criteria of Waldenstrom Macroglobulinemia (WM) revised in the 4th edition of the WHO classification in 2008, the definition of WM combines the morphological diagnosis of bone marrow involvement of lymphoplasmacytic lymphoma (LPL) with IgM paraproteinemia (9
). Owing to histological overlapping between lymphoplasmacytic lymphoma and other mature B-cell malignancies with plasmacytic differentiation, immunophenotyping is invaluable in the differential diagnosis of B-cell lymphoproliferative disorders, and in all cases of suspected WM it is needed to apply (5
). The special immunophenotypic profile for lymphoplasmacytic cells in WM include the expression of monoclonal immunoglobulin light chain (κ and λ and the pan B-cell antigens CD19+CD20+ (1
). The majority of cases have CD5-CD10-CD23-, but 5%-20% of patients appear to express the CD5 antigen (10
). Although the expression of CD5+ in LPL/WM is uncommon, this should not preclude a diagnosis of WM. However, the attention should be paid to diagnose the CD5+ cases to exclude chronic lymphocytic leukemia and mantle cell lymphoma.
In bone marrow examination of our patient, small lymphoid cells expressed CD5+CD20+ and positive reaction in small numbers of plasma cells with anti-cytoplasmic IgM antibody was observed. Because of CD5+ small lymphoid cells, the careful attention for symptoms and signs was important to differentiate from other B-cell lymphoproliferative disorders. Comparing to chronic lymphocytic leukemia, peripheral blood lymphocyte count of this patient was not compatible with the diagnostic criteria of the International Workshop on CLL (IW-CLL) (11
). In mantle cell lymphoma, swelling of lymph nodes are usually present and gastrointestinal tract involvement occurs in a very high percentage and the majority of patients have genetic abnormalities such as t(11:14)(q13;q32) (3
). This patient did not have lymphadenopathy and extranodal involvement in PET-CT and also did not have chromosome abnormalities. Instead of these characteristics of other B-cell lymphoproliferative disorders, this patient complained of hematuria, pretibial pitting edema, numbness of hand and foot in cold weather, and visual disturbance (13
Renal involvement of WM is a rare manifestation, but a wide spectrum of renal lesions is observed. After the first description of human renal lesions associated with deposition of monoclonal lights chains, several renal complications have been reported (7
). WM-related nephropathies are characteristic intracapillary deposits of IgM. In renal biopsy of our patient, we found pseudothrombi and subendothelial hyaline deposits with stained IgM and subendothelial and mesangial electron dense deposits revealing microtubular structures on electron microscopy compatible with cryoglobulinemic nephropathy (14
). Serum cryoglobulin of this patient was positive. Although there are a number of systemic conditions associated with cryoglobulinemia, including hepatitis C, lymphoproliferative disorders, infections, systemic lupus erythematosus (SLE) and other collagen vascular diseases, this patient was not associated with them. In WM, cryoglobulinemic glomerulonephropathy is a very rare renal disease which was reported in only 2 cases worldwide (7
In addition to renal disease, the patient complained of visual disturbance. Ophthalamic examination showed vascular segmentation and dilatation of the retinal veins. These revealed hyperviscosity syndrome (6
). Hyperviscosity is a main feature in WM patients, and is due to the excess of IgM paraproteins. The relationship between serum viscosity and clinical manifestations is not precise, and a general absolute value for viscosity at which hyperviscosity becomes clinically relevant does not exist (15
). And it is difficult to use commercial check of viscosity in Korea, so we couldn't run the tape over serum viscosity level.
WM usually follows a relatively indolent course with a median survival ranging from 60 to 120 months in different series (16
). However, in some patients the disease may be more aggressive (16
). Several analyses have identified variables that may be associated with a worse prognosis. These include advanced age, cytopenia(s), hypoalbuminemia, elevated serum β2-microglobulin, high IgM, poor performance status, B-symptoms or splenomegaly. An International Prognositc Scoring System for WM (IPSSWM) was formulated based on 5 adverse covariates: age > 65 yr, hemoglobin less than or equal to 11.5 g/dL, platelet count less than or equal to 100,000/µL, β2-microglobulin more than 3 mg/L, and serum monoclonal protein concentration more than 7.0 g/dL. The risk group is categorized as having low, intermediate, high risk and five-year survival rates of these groups are 87%, 68%, and 36% respectively (17
). Meanwhile, the indications for immediate therapy are disease-related cytopenias, bulky adenopathy or organomegaly, symptomatic hyperviscosity, severe neuropathy, amyloidosis, cryoglobulinemia, cold agglutinin disease, or evidence of disease transformation (13
). There is no standard therapy for the treatment of symptomatic WM. Recent studies recommended the combination of rituximab with nucleoside analogs and/or alkylating agents or with cyclophosphamide-based therapies, or the combination of rituximab with thalidomide (13
). An alternative frontline regimen can be considered along with bortezomib, alemtuzumab and stem cell transplantation. Newer agents, such as bendamustine and everolimus, can also be considered in the treatment of WM (6
). According to IPSSWM, our patient is high risk group based on hemoglobin, platelet count and β2-microglobulin level. However, at present, no data suggest that new targeted therapies may overcome an adverse prognostic factor in WM and rituximab-based chemotherapy has economic problem in Korea. So we treated this patient with CHOP chemotherapy and after the treatment, the patient was in complete remission.
In conclusion, WM associated with CD5+ LPL and presented as cryoglobulinemic glomerulonephropathy is very rare, so clinical features are important for differential diagnosis in CD5+ B-cell lymphoproliferative disorders. Conventional chemotherapy with CHOP is effective in symptomatic high risk group with cryoglobulinemia in WM.