Alterations in AMPA receptor subunits and TARPs in the rat nucleus accumbens related to the formation of Ca2+-permeable AMPA receptors during the incubation of cocaine craving

doi:10.1016/j.neuropharm.2011.01.021

Neuropharmacology. Author manuscript; available in PMC 2012 December 1.

Published in final edited form as:

Neuropharmacology. 2011 December; 61(7): 1141–1151.

Published online 2011 January 27. doi: 10.1016/j.neuropharm.2011.01.021

PMCID: PMC3094740

NIHMSID: NIHMS269233

Alterations in AMPA receptor subunits and TARPs in the rat nucleus accumbens related to the formation of Ca²⁺-permeable AMPA receptors during the incubation of cocaine craving

Carrie R. Ferrario,^a¹ Jessica A. Loweth,^a Mike Milovanovic,^a Kerstin A. Ford,^a Gregorio L. Galiñanes,^b² Li-Jun Heng,^b Kuei Y. Tseng,^b and Marina E. Wolf^a

^aDepartment of Neuroscience, Rosalind Franklin University of Medicine and Science, 3333 Green Bay Road, North Chicago, Il 60064-3095

^bDepartment of Cellular and Molecular Pharmacology, Rosalind Franklin University of Medicine and Science, 3333 Green Bay Road, North Chicago, Il 60064-3095

Corresponding author: Marina E. Wolf, Department of Neuroscience, Rosalind Franklin University of Medicine and Science, 3333 Green Bay Road, North Chicago, Il 60064-3095. Tel: (847) 578-8659 Fax: (847) 578-8515, Email: marina.wolf/at/rosalindfranklin.edu

¹Present address: Pharmacology Department, The University of Michigan, 1000 Wall Street, Ann Arbor, MI, 48105, ferrario/at/umich.edu

²Present address: Laboratorio de Fisiología de Circuitos Neuronales, Departamento de Fisiología y Biofísica, Facultad de Medicina, Universidad de Buenos Aires, Ciudad de Buenos Aires C1121ABG, Argentina. neurofis/at/fmed.uba.ar

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Abstract

Cue-induced cocaine seeking intensifies or incubates after withdrawal from extended access cocaine self-administration, a phenomenon termed incubation of cocaine craving. The expression of incubated craving is mediated by Ca²⁺-permeable AMPA receptors (CP-AMPARs) in the nucleus accumbens (NAc). Thus, CP-AMPARs are a potential target for therapeutic intervention, making it important to understand mechanisms that govern their accumulation. Here we used subcellular fractionation and biotinylation of NAc tissue to examine the abundance and distribution of AMPAR subunits, and GluA1 phosphorylation, in the incubation model. We also studied two transmembrane AMPA receptor regulatory proteins (TARPs), γ2 and γ4. Our results, together with earlier findings, suggest that some of the new CP-AMPARs are synaptic. These are probably associated with γ2, but they are loosely tethered to the PSD. Levels of GluA1 phosphorylated at serine 845 (pS845 GluA1) were significantly increased in biotinylated tissue and in an extrasynaptic membrane-enriched fraction. These results suggest that increased synaptic levels of CP-AMPARs may result in part from an increase in pS845 GluA1 in extrasynaptic membranes, given that S845 phosphorylation primes GluA1-containing AMPARs for synaptic insertion and extrasynaptic AMPARs supply the synapse. Some of the new extrasynaptic CP-AMPARs are likely associated with γ4, rather than γ2. The maintenance of CP-AMPARs in NAc synapses during withdrawal is accompanied by activation of CaMKII and ERK2 but not CaMKI. Overall, AMPAR plasticity in the incubation model shares some features with better described forms of synaptic plasticity, although the timing of the phenomenon and the persistence of related neuroadaptations is significantly different.

1. Introduction

Relapse to cocaine use, even after a long period of abstinence, is a critical problem in treating addiction. Relapse is often triggered by craving elicited by cues or contexts previously associated with cocaine (O’Brien et al., 1992). A number of different models exist for studying cue-induced cocaine craving in rats (e.g., Crombag et al., 2008). We are particularly interested in the incubation model. Incubation refers to the progressive intensification of cue-induced cocaine-seeking that occurs after withdrawal from extended access cocaine self-administration. Enhanced drug-seeking in this rat model persists >3 months after the last cocaine exposure (Lu et al., 2004). The incubation model is particularly relevant to human cocaine users who undergo prolonged abstinence due to incarceration or hospitalization, and then, once released, encounter previously drug-paired cues that can trigger relapse (Reichel and Bevins, 2009).

Our studies focus on the role of AMPA-type glutamate receptors (AMPARs) in the incubation of cocaine craving. AMPARs are tetramers composed of GluA1-4 subunits (Shepherd and Huganir, 2007). They are associated with auxiliary subunits, including a family of transmembrane AMPA receptor regulatory proteins (TARPs) that regulate AMPAR trafficking, channel properties, and glutamate affinity (Kato et al., 2010). Medium spiny neurons, the output neurons of the nucleus accumbens (NAc), are excited primarily by AMPARs. This is required for drug seeking in many rat models of cocaine addiction, suggesting AMPAR transmission in the NAc as a key control point for craving and relapse (Kalivas, 2009; Wolf and Ferrario, 2010). Therefore, it is important to compare AMPAR transmission in the NAc of drug-naïve rats and rats that have undergone incubation (“incubated rats”).

While most AMPARs in the brain contain the GluA2 subunit, there has been considerable recent interest in AMPARs that lack this subunit; these receptors are Ca²⁺-permeable (CP-AMPARs) and have higher conductance than GluA2-containing, Ca²⁺-impermeable AMPARs (CI-AMPARs; Cull-Candy et al., 2006; Isaac et al., 2007; Liu and Zukin, 2007). Our recent studies have shown that CP-AMPARs are expressed at very low levels in NAc synapses of adult drug-naïve rats (Conrad et al., 2008; Reimers et al., 2010), but accumulate in association with the incubation of cue-induced cocaine craving and mediate its expression in rats tested on withdrawal day (WD) 45 (Conrad et al., 2008). The presence of CP-AMPARs in NAc synapses of “incubated rats” was demonstrated using electrophysiological techniques, a bis(sulfosuccinimidyl)suberate (BS³) protein crosslinking assay showing increased cell surface levels of GluA1 but not GluA2, and co-immunoprecipitation experiments showing an increase in levels of GluA1 not physically associated with other AMPAR subunits (Conrad et al., 2008). Although we first demonstrated CP-AMPARs in the NAc core of “incubated rats”, we recently showed that they are also present in the rat NAc shell on WD35-49 (McCutcheon et al., 2010). CP-AMPARs have also been detected in the mouse NAc shell after 35 days of withdrawal from extended access cocaine self-administration (Mameli et al., 2009).

The goal of the present study was to learn more about the subcellular distribution of CP-AMPARs in the NAc of “incubated rats” and to understand how these receptors gain access to NAc synapses and accumulate there during withdrawal. To this end, we used subcellular fractionation and biotinylation to examine the abundance and subcellular distribution of AMPAR subunits, as well as GluA1 phosphorylated at serine 845 (pS845 GluA1), in NAc tissue obtained from control rats or “incubated rats” on WD30-45. We also studied two TARPs, γ2 and γ4, that we have previously shown are expressed in the adult rat NAc (Ferrario et al., in press). No prior studies have evaluated the effect of cocaine (or other drugs of abuse) on TARP expression or distribution. Finally, we measured the activation of several signaling pathways previously linked to AMPAR plasticity.

2. Methods

2.1 Subjects and surgical procedures

All procedures were approved by the Rosalind Franklin University of Medicine and Science Animal Care and Use Committee. Ninety-five Male Sprague Dawley rats (Harlan; 250–275g) were individually housed (12:12 dark/light). All self-administration sessions were conducted in the dark phase of the cycle with food and water available continually. For catheter implantation, rats were anesthetized with isoflurane gas (Henry Schein, Melville, NY) and given the analgesic banamine (2 mg/kg s.c.) prior to surgery. A silastic catheter (Plastics one, Roanoke, VA) was inserted into the right jugular vein, passed under the skin over the right shoulder and fixed in the mid-scapular region. Rats were given 7 days to recover from surgery prior to beginning self-administration. During this time, catheters were flushed every 1–2 days with sterile saline (0.1 ml, 0.9%).

2.2 Cocaine self-administration

Seven days after surgery, rats began self-administration sessions. Sessions (6 h) were conducted once per day for 10 consecutive days, as in our previous study (Conrad et al., 2008). Self-administration chambers (MED Associates, St. Albans, VT) were located within sound and light attenuating cabinets and were equipped with 2 nose-poke ports located on opposite sides of the chamber, 2 cm above the floor. Responding in one port (active) resulted in the delivery of an intravenous infusion of cocaine (0.5 mg/kg in 32 µl; Coc-SA) or saline (32 µl/infusion; Sal-SA) and the illumination of a cue light inside the port. Responses in the second port (inactive) had no consequences. Infusions were delivered on a fixed ratio 1 schedule with a 10 sec time out for the first 10 infusions or first hour (whichever occurred first) followed by a 30 sec time out for the remainder of the session. The number of responses in the active and inactive ports and the number of infusions obtained were recorded throughout each session. Additional handled control rats (Han) were treated in an identical manner as self-administration rats, except they were not exposed to the self-administration chambers. Instead, after recovery from catheter implantation surgery, they were brought to holding cages away from their colony room where they remained for 6 h before being returned home. This was repeated on 10 consecutive days. These rats were also flushed with saline before and after being placed in the holding cages. Thus, handled controls rats experienced the same surgery, recovery, time away from the colony and handling as self-administration groups. Comparisons between Coc-SA, Sal-SA and Han groups were made for electrophysiological experiments. Comparisons between Coc-SA and Han groups were used in the lysed synaptosomal membrane (LP1) fraction. For all other subcellular fractionation experiments and biotinylation experiments, comparisons were made between Coc-SA and Sal-SA groups. Sal-SA and Han control groups were used interchangeably because no differences between these groups were found in electrophysiological experiments assessing the presence of CP-AMPARs (see Section 3.2). Furthermore, in biochemical studies, levels of GluA1 and GluA2 proteins in various fractions did not differ between these two groups (data not shown). Similar Han control groups have been used in previous studies (Ferrario et al., 2005; Briand et al., 2008a,b). For biochemical studies, tissue was collected on WD1, WD35 or WD45 following the last self-administration or handling session. Electrophysiological studies were conducted on WD30-33. Details of groups compared, N values and withdrawal times are provided below following the detailed methods for each type of experiment.

2.3 Whole-cell patch-clamp recordings

Whole-cell patch-clamp recordings were conducted in the presence of NMDAR (APV) and GABA_AR (picrotoxin) antagonists as previously described (Conrad et al., 2008). Briefly, the rats were anesthetized with chloral hydrate (400 mg/kg, i.p.) before being decapitated. Brains were rapidly removed into ice-cold artificial cerebral spinal fluid (aCSF) containing (in mM): 125 NaCl, 25 NaHCO₃, 12.5 glucose, 3.5 KCl, 1.25 NaH₂PO₄, 0.5 CaCl₂, 3 MgCl₂, 0.05 APV, and 0.05 picrotoxin (pH 7.45, 295–305 mOsm). Coronal slices (300 µm thick) containing the NAc were cut in ice-cold aCSF with a Vibratome, and incubated in warm (~35°C) aCSF solution constantly oxygenated with 95% O₂-5% CO₂ for at least 60 min before recording. In the recording aCSF (delivered at 2 ml/min), CaCl₂ was increased to 2 mM and MgCl₂ was decreased to 1 mM. Patch pipettes (6–9 MΩ) were pulled from 1.5 mm borosilicate glass capillaries (WPI, Sarasota, FL) with a horizontal puller (Model P97, Sutter Instrument, Novato, CA), and filled with a solution containing 0.125 % Neurobiotin and (in mM): 140 Cs-gluconate, 10 HEPES, 2 MgCl₂, 3 Na₂-ATP, 0.3 GTP, 0.1 spermine, 1 QX-314 (pH 7.3, 280–285 mOsm). All chemicals and drugs were purchased from Sigma-Aldrich. Experimental groups used for patch-clamp recording studies: Han (N=6 cells from 3 rats), Sal-SA (N=5 cells from 2 rats), Coc-SA WD30-33 (N=5 cells from 2 rats).

2.4 Tissue collection

One, 35, or 45 days after the last self-administration session (or transport to holding cages for Han group), rats were decapitated and bilateral samples of the NAc (core and shell) were rapidly dissected from a 2 mm coronal section obtained using a brain matrix (Activational Systems, Warren, MI).

2.5 Preparation of LP1 fraction and postsynaptic density fraction (PSD)

The subcellular fractionation method used to obtain these fractions was adapted from Goel et al. (2006). NAc tissue was homogenized (Wheaton Potter-Elvehjem Tissue Grinders, Fisher Scientific, Pittsburgh, PA) in HEPES-buffered sucrose (0.32 M sucrose, 4 mM HEPES, pH 7.4) containing 2 mM EGTA, 50 mM NaF, 10 mM PPi, 1 mM NaOV, 1uM okadaic acid, 1 mM phenylmethyl sulfonyl fluoride (PMSF), 1 uM microcystin-LF and 1X protease inhibitor cocktail set 1 (Calbiochem, Darmstadt, Germany). The homogenate was centrifuged (800 × g, 10 min, 4°C) to remove the pelleted nuclear fraction (P1). The resulting supernatant (S1) was centrifuged (10,000 × g, 15 min, 4°C) to yield a crude membrane fraction (P2) which was washed by resuspending in HEPES-buffered sucrose containing protease and phosphatase inhibitors and centrifuging again. The washed P2 fraction was lysed hypo-osmotically with cold 4 mM HEPES (pH 7.4) with inhibitors and centrifuged (25,000 × g, 20 min, 4°C) to yield the LP1 fraction, which was re-suspended in HEPES-buffered sucrose and stored at −80°C. Experimental groups used for analysis of the LP1 fraction: Han (N=10) and Coc-SA WD45 (N=9).

For PSD preparation, the LP1 was re-suspended in HEPES-buffered sucrose with inhibitors and run on a discontinuous sucrose gradient (1.2 M, 1.0 M, and 0.8 M sucrose with inhibitors) and centrifuged (125,000 × g, 2 h, 4° C). The synaptic plasma membrane fraction (SPM) was collected between 1.0 M and 1.2 M sucrose. The SPM was diluted 2.5 times in 4 mM HEPES with inhibitors and pelleted by centrifugation (150,000 × g, 30 min, 4°C). The SPM pellet was re-suspended in a 0.5% Triton X-100, HEPES-EDTA solution (50 mM HEPES, 2 mM EDTA, pH 7.4 with inhibitors), incubated with rotation (15 min, 4°C) and centrifuged (32, 000 × g, 20 min, 4°C) to pellet the insoluble postsynaptic density fraction (PSD). The PSD fraction was resuspended in Laemmli sample treatment buffer containing 100mM dithiothreitol (DTT). Samples were then stored at −80°C until used for SDS-PAGE and Western blotting. Experimental groups used for analysis of PSD fraction: Sal-SA WD1 (N=9), Coc-SA WD1 (N=9), Sal-SA WD45 (N=9), and Coc-SA WD45 (N=7).

2.6 Preparation of a fraction enriched for extrasynaptic membranes

We used a previously described subcellular fractionation procedure (Davies et al., 2007; 2008; Goebel-Goody et al., 2009) that we have validated in our laboratory (Ferrario et al., in press). Each NAc sample was homogenized in 3 ml of sucrose homogenization buffer (10mM HEPES, 0.32M sucrose, 5mM NaF, 1mM NaVO, 2 mM EDTA, pH 7.4) in a glass grinding vessel with a rotating Teflon pestle (Wheaton Overhead Stirrer; 3000 RPM for 12 passes). The homogenate was then processed as described above to yield the P2 fraction. The P2 fraction was washed twice with sucrose homogenization buffer and re-suspended in 4 ml of HEPES-buffered sucrose, containing 0.5% Triton X-100 (0.5% v/v), using a motorized pellet pestle mixing/grinding rod (Kontes, Vineland, NJ) in an ultracentrifuge tube. The suspension was then incubated with gentle rotation (20 min, 4°C) and centrifuged (32,000 × g, 20 min) to yield the insoluble pellet (enriched for synaptic membranes) and the soluble fraction (enriched for extrasynaptic membranes). Both fractions were used for initial validation studies in tissue from drug-naïve rats (Ferrario et al., in press) but the insoluble fraction was not analyzed for the present experimental groups because it is redundant with the PSD fraction described in Section 2.5. The extrasynaptic membrane fraction was concentrated by adding 8 volumes of cold acetone, incubating overnight (−20°C), and centrifuging (3000 × g). The concentrated pellet was solubilized in sucrose-Triton buffer containing 1% SDS. The samples were stored at −80°C. For SDS-PAGE analysis of extrasynaptic membrane fractions, 5 ug of protein per lane was loaded onto gels. Samples were stored at −80°C until analysis. Experimental groups used for analysis of extrasynaptic membranes: Sal-SA WD45 (N=12); Coc-SA WD45 (N=8).

2.7 Biotinylation

Bilateral pieces of NAc tissue from each rat were dissected using a brain matrix and chopped into 400 µm slices using a McIllwain tissue chopper (The Vibratome Company, O’Fallon, MO). The tissue was added to eppendorf tubes containing ice-cold aCSF with 1 mM sulfo-NHS-S-S-Biotin (Thermo Scientific, Rockford, IL) and incubated at 4°C with gentle agitation for 30 min. Samples were then incubated at 4°C for 10 min in glycine (100 mM) to quench the reaction. The tissue was pelleted, re-suspended in ice-cold lysis buffer [25 mM HEPES pH 7.4, 500 mM NaCl, 2 mM EDTA, 20 mM NaF, 10 mM NaPPi,1 mM PMSF, 0.1% NP-40 (v/v), 1mM NaOV, 1 uM okadaic Acid, 1 uM microsystin-LF, 1X protease inhibitor cocktail (Calbiochem; 539131)], sonicated, and stored at −80°C as described previously for protein crosslinking experiments (Boudreau and Wolf, 2005; Ferrario et al., 2010). Biotinylation and protein crosslinking experiments are identical except for the fact that different reagents (sulfo-NHS-S-S-Biotin and BS³, respectively), are used to selectively modify cell surface proteins. Procedures for purifying biotinylated proteins were adapted from Thermo Scientific product instructions (http://www.piercenet.com/files/0682as4.pdf). Protein concentrations were determined using the Bradford Assay. An aliquot of each NAc sample (100 µg) was added to 37.5 µl of high capacity NeutrAvidin agarose beads (Cat. No. 29204, Thermo Scientific) and incubated overnight at 4°C on an end-over-end rotator. Biotinylated proteins bound to NeutrAvidin beads (bound fraction) were isolated from the nonbiotinylated (unbound) fraction by centrifugation (3,000 RPM, 1 min) and washed several times in 1X PBS. The biotinylated fraction was then dissolved in Laemmli sample buffer with DTT (100 mM) and heated at 97°C for 3 min to release the biotinylated proteins from the beads. The samples were then spun at 10,000 RPM for 5 min on a centrifugal filter unit (0.45 µm, #UFC30HV00, Millipore, Billerica, MA) to remove the NeutrAvidin beads from the solution. The samples were then used for immunoblotting as described in Section 2.8. Experimental groups used for analysis of biotinylated tissue: Sal-SA WD35 (N=12); Coc-SA WD35 (N=9).

2.8 SDS-PAGE and immunoblotting

All samples were heated at 70°C for 10 min in Laemmli sample treatment buffer with 100mM DTT and then processed for SDS-PAGE and immunoblotting as described previously (Ferrario et al., 2010) using the following primary antibodies: GluA1 (1:1000, PA1-37776, Thermo Scientific or 1:1000, AB1504 or MAB2263, Millipore); pS845 GluA1 (1:500, p1160-845, PhosphoSolutions, Aurora, CO; or 1:500, 2491-1, Epitomics, Burlingame, CA); GluA2 (1:200, 75-002, UC Davis/NIH NeuroMab Facility, Davis, CA or 1:1000, AB1768, Millipore); GluA3 (1:500, 3437, Cell Signaling, Danvers, MA); γ2 (1:1000, 1505-STAR, PhosphoSolutions); γ4 (1:1000, AB5795, Millipore); PSD-95 (1:20,000, 75-028, UC Davis/NIH NeuroMab Facility); CaMKIIα (1:20,000, MAB8699, Millipore); CaMKIIβ (1:500, ab34703; AbCam, Cambridge, MA), pCaMKIIα/β (1:5000, p1005-286, PhosphoSolutions); total ERK1/2 (1:10,000, AB3053, Millipore); pERK1/2 (1:10,000, AB3826, Millipore). Total and phospho-CaMKI antibodies were the generous gift of Dr. Naohito Nozaki (Department of Biochemistry and Molecular Biology, Kanagawa Dental College, Kanagawa, Japan). Membranes were washed in TBS-T solution, incubated at RT for 60 min with HRP-conjugated anti-rabbit or mouse IgG (1:10,000; Invitrogen, Carlsbad, CA), washed with TBS-T, and rinsed with ddH₂O. Membranes were then immersed in chemiluminescence (ECL) detecting substrate (GE Healthcare, Piscataway, NJ). Images of immunoblots were taken on film and quantified using Total Lab (Life Sciences Analysis Essentials). A background value was obtained and diffuse densities for bands of interest in each lane were determined. Because the PSD sample is solubilized directly in SDS, measures of protein concentration in the PSD fraction were unreliable. Therefore, PSD samples were loaded according to volume. All other samples were loaded according to protein concentration. For LP1 and biotinylated (surface) samples, diffuse densities were normalized to total protein in the lane determined by Ponceau-S stain. For data obtained using extrasynaptic membrane fractions, diffuse densities were normalized to µg of protein loaded as described previously (Ferrario et al., in press) because Ponceau-S staining was too weak. Comparisons were made between experimental and control groups using one-way ANOVAs and unpaired t-tests when appropriate. In all cases, Coc-SA groups were compared to a Sal-SA or Han control group killed on the same day, in order to avoid variability due to potential differences between control groups (e.g., related to age or duration of single housing). Data are presented as mean ± S.E.M. expressed as percent of control. For some proteins, we were unable to quantify immunoreactivity for some lanes due to an unclear band, smudge on the blot or air bubble obstructing the signal. Therefore, the N for western blotting data may vary from the total number of rats treated. Degrees of freedom are given throughout so that N can be determined.

3. Results

3.1 Self-administration

Rats self-administered cocaine for 6 h/day for 10 days, as in our previous study (Conrad et al., 2008). All of these rats (Coc-SA groups) readily acquired cocaine self-administration and no significant differences in drug intake were found between different cohorts of rats (data not shown). Controls either self-administered saline (Sal-SA groups) or were treated identically to Sal-SA rats except for exposure to the self-administration chamber (Han groups); see Methods for more details. Fig. 1A shows the average number of infusions of cocaine (closed squares) or saline (open circles) taken per session from all four self-administration cohorts.

Figure 1

Self-administration behavior and whole-cell patch-clamp recordings demonstrating presence of CP-AMPARs. A) Mean number of infusions (± SEM) taken during each 6 hour self-administration session for saline (N=47; open circles) and cocaine (N=48; (more ...)

3.2 Electrophysiological demonstration of CP-AMPARs in NAc of Coc-SA rats on WD30-33

After prolonged (42–47 days) but not short (1 day) withdrawal from the same cocaine self-administration used herein, we demonstrated that CP-AMPARs are incorporated into excitatory synapses on medium spiny neurons of the rat NAc core (Conrad et al., 2008). In the current study, we confirmed these results by randomly selecting Han, Sal-SA and Coc-SA rats from one cohort of rats destined for biochemical studies. NAc slices were obtained from these rats on WD30-33 and used for whole-cell patch-clamp recordings of medium spiny neurons in the NAc core. The presence of CP-AMPARs was evaluated from the rectification index (RI), defined as the ratio of evoked excitatory postsynaptic currents (EPSCs) recorded at −70mV and +40mV. Both CP- and CI-AMPARs contribute to EPSC_−70mV whereas only CI-AMPARs are responsible for EPSC_+40mV due to voltage-dependent block of CP-AMPARs by intracellular polyamines. Thus, an increase in the RI indicates a higher synaptic content of CP-AMPARs. As expected from our past results, neurons from all Coc-SA rats showed a significantly higher RI than neurons from Sal-SA and Han groups (Fig. 1B; one-way ANOVA: F_(1,2) = 16.6, p<.001; post-hoc Han vs COC-SA and SAL-SA vs Coc-SA: p values <.0001). These data confirm that the cocaine self-administration regimen used here reliably leads to incorporation of CP-AMPARs into NAc synapses and that these receptors can be detected as early as WD30. It should be noted that no differences were observed between neurons recorded from Sal-SA and Han control groups.

3.3 Analysis of AMPAR levels in PSD and LP1 fractions prepared from the NAc

Based on electrophysiological results indicating a contribution of CP-AMPARs to synaptic currents after prolonged withdrawal, and biochemical data indicating that these receptors are GluA1-containing (Conrad et al., 2008), we predicted that Coc-SA rats should show an increase in GluA1 protein in the PSD fraction. To test this, we prepared PSD fractions from the NAc of four experimental groups: Sal-SA WD1, Sal-SA WD45, Coc-SA WD1 and Coc-SA WD45. To our surprise, no significant differences in GluA1 total protein levels were found. In fact, a slight decrease in GluA1 protein in the PSD was detected in the Coc-SA WD45 group (Fig. 2A). In addition, no changes in GluA2 or GluA3 protein levels in the PSD were evident (Fig. 2C,D). Next we examined two TARPs, γ2 and γ4, which we have previously shown to be enriched in synaptic and extrasynaptic membranes, respectively, in the NAc of drug-naïve adult rats (Ferrario et al., in press). The Coc-SA WD45 group showed a trend towards decreased γ2 levels in the PSD (Fig. 2B, inset), whereas γ4 could not be detected in the PSD (data not shown), consistent with its primarily extrasynaptic location in the NAc (Ferrario et al., in press). Interestingly, γ2 levels were correlated with GluA1 levels, but not with GluA2 or GluA3 levels, in all groups. Accordingly, the loss of GluA1 in the Coc-SA WD45 group was accompanied by a similar loss of γ2 (Fig. 2B; r=.68, p<.05). Given that CP-AMPARs are readily detected in NAc synapses of “incubated rats” using electrophysiological techniques (Fig. 1B; Conrad et al., 2008) and that increases in cell surface and total GluA1 have also been detected in these rats (Conrad et al., 2008 and below), we propose that the decrease in GluA1 in the PSD fraction is due to weak anchoring of CP-AMPARs at the synapse compared to GluA2-containing AMPARs, resulting in their loss during the preparation of the PSD fraction.

Figure 2

Expression of AMPAR subunits and the TARP γ2 in the NAc postsynaptic density (PSD) fraction after 1 or 45 days of withdrawal from self-administration. Data are presented as mean (± SEM) expressed as percent of saline controls at each withdrawal (more ...)

To evaluate this possibility, we prepared an LP1 fraction, hypothesizing that loosely anchored receptors would be retained in this cruder membrane fraction (which has not been exposed to detergent; see Methods). Consistent with our earlier findings (Conrad et al., 2008), GluA1 protein levels in the LP1 fraction were significantly increased on WD45 in the NAc of the Coc-SA group compared to Han controls (Fig. 3A; t₁₇=1.7, p<.05). Levels of the TARPs γ2 and γ4 were also elevated in the LP1 fraction of the Coc-SA group, although these trends did not reach statistical significance (Fig. 3B). Furthermore, GluA2 levels were unaltered (Fig. 3C) whereas GluA3 levels were decreased (Fig. 3D; t₁₇=2.1, p=0.05). These data are consistent with the addition of GluA1-containing CP-AMPARs (and associated TARPs) to synaptic membranes during incubation. Furthermore, they support our hypothesis that the decrease in GluA1 in the PSD fraction of the Coc-SA WD45 group was a result of steps leading to PSD purification.

Figure 3

Expression of AMPAR subunits and the TARPs γ2 and γ4 in the NAc lysed synaptosomal membrane (LP1) fraction after 45 days of withdrawal from cocaine-administration (Coc-SA WD45) or the last handling session (Han). Data are presented as (more ...)

3.4 Analysis of AMPAR surface expression in the NAc using biotinylation

In our initial study (Conrad et al., 2008), we found an increase in surface expression of GluA1 (but not GluA2) in Coc-SA rats on WD45 using a BS³ protein crosslinking assay. We wanted to confirm this result in the present study and also evaluate surface expression of pS845 GluA1 (based on the role of S845 phosphorylation in the insertion of GluA1 into extrasynaptic regions of the plasma membrane; see Discussion) and the TARPs γ2 and γ4. Unfortunately, despite advantages of the BS³ assay with respect to accuracy and efficiency (Boudreau and Wolf, 2005), we could not accomplish our present goals using this assay because phospho-specific GluA1 antibodies and TARP antibodies do not recognize their targets after BS³ crosslinking (data not shown). As an alternative approach, we biotinylated NAc tissue from Coc-SA and Sal-SA groups killed on WD35. The biotinylated fraction, containing surface-expressed proteins, was immunoblotted using GluA1 and GluA2 antibodies. Cell surface GluA1 was significantly increased in the Coc-SA compared to the Sal-SA group (Fig. 4A; t₁₆= 2.8, p<.05), while total GluA2 was not changed (Fig. 4A). These data confirm previous BS³ crosslinking results indicating an increase in cell surface GluA1 but not GluA2 in the Coc-SA group (WD45; Conrad et al., 2008) and are consistent with electrophysiological evidence for CP-AMPARs on WD30-33 (Fig. 1B) and the selective increase in GluA1 protein in the LP1 fraction on WD45 (Fig. 3). We note that the increase in surface GluA1 detected with biotinylation on WD35 is smaller than the increase found in our prior BS³ crosslinking studies. Thus, GluA1 surface values for individual rats in the biotinylation experiment ranged from 109–164% of control, whereas values in the prior crosslinking experiments ranged from 129–304% of control (4 experimental cohorts; Conrad et al., 2008). Differences in the magnitude of the GluA1 increase could be due to differences in tissue processing between biotinylation and BS³ crosslinking, the GluA1 antibody used (the GluA1 antibody employed in Conrad et al., 2008 was no longer useable at the time the present experiments were conducted), or the withdrawal day (WD30-33 versus WD42-47; however it should be noted that the RI did not differ between these withdrawal times, as shown in Fig. 1B). Total drug intake did not vary significantly between the Coc-SA cohorts in the present study and in Conrad et al. (2008).

Figure 4

Surface expression of AMPAR subunits and the TARPs γ2 and γ4 after 35 days of withdrawal from self-administration, determined using biotinylation. Data are presented as mean (± SEM) expressed as percent of saline self-administering (more ...)

Interestingly, in addition to an increase in surface GluA1 on WD35, immunoblotting analysis of the biotinylated fraction with a phospho-specific antibody revealed a significant increase in pS845 GluA1 levels in the Coc-SA group compared to the Sal-SA group (Fig. 4A; t₁₅=2.7, p<.05). Because both surface GluA1 and pS845GluA1 increased in the Coc-SA group, their ratio (pS845 GluA1/total GluA1) did not differ between groups (Fig. 4A). These results suggest that new GluA1-containing AMPARs added to the cell surface during cocaine withdrawal are phosphorylated on serine 845. Surface expression of γ4 was also significantly increased in the Coc-SA group compared to the Sal-SA group (Fig. 4B; t₁₇= 2.4, p<.05), whereas γ2 levels were unchanged (Fig. 4B). This change in γ4 and not γ2 levels is particularly interesting considering that γ2 moved in parallel to GluA1 in the PSD fraction and that γ4 is not readily detectable in synaptic membranes in the NAc (Ferrario et al., in press; see Discussion)

3.5 Analysis of AMPAR subunits in extrasynaptic membranes from the NAc

The biotinylation studies described in Section 3.4 show that GluA1-containing AMPARs (phosphorylated at S845) increase on the cell surface after cocaine withdrawal, but this approach does not discriminate between GluA1 located at synaptic versus extrasynaptic sites. Electrophysiological data indicate that some of the new CP-AMPARs are found in synapses after cocaine withdrawal, but the extrasynaptic accumulation of CP-AMPARs could contribute to the observed increase in surface and LP1 levels of GluA1. Extrasynaptic CP-AMPARs are also of interest because they may supply the synaptic CP-AMPAR pool (see Discussion). To assess the possibility that some CP-AMPARs accumulate at extrasynaptic sites, we used a previously described subcellular fractionation procedure to isolate an extrasynaptic membrane-enriched fraction (Davies et al., 2007, 2008; Goebel-Goody et al., 2009). We have previously validated this procedure in our own laboratory by analyzing levels of marker proteins for different subcellular compartments. For example, synaptic markers [PSD-95 and Ca²⁺/calmodulin-dependent protein kinase II (CaMKII)] are virtually undetectable in extrasynaptic membranes. In addition, we used this protocol to demonstrate that extrasynaptic membranes from the NAc of drug-naïve rats contain GluA1-3, albeit at lower levels than found in synaptic membranes, and that homomeric GluA1 receptors can be detected in extrasynaptic membranes (Ferrario et al., in press).

Fig. 5 shows immunoblotting results obtained using extrasynaptic membranes from the NAc of Coc-SA and Sal-SA groups killed on WD45. Although GluA1 levels in this fraction did not increase significantly in the Coc-SA WD45 group, we did find a significant increase in the ratio of pS845 GluA1 to total GluA1 protein levels (Fig. 5; t₁₅=2.1, p<.05). Taken along with the increase in GluA1 protein on the surface and in the LP1 fraction, these data may suggest an increase in the fraction of extrasynaptic GluA1-containing AMPARs primed for synaptic insertion by S845 phosphorylation (see Discussion).

Figure 5

Levels of total GluA1 and GluA1 phosphorylated on S845 (pS845 GluA1) in an extrasynaptic membrane-enriched fraction prepared after 45 days of withdrawal from self-administration. Data are presented as mean (± SEM) expressed as percent of saline (more ...)

3.6 Analysis of signaling pathways in NAc PSD fractions

Using the same PSD fractions used for analysis of AMPAR subunits (Fig. 2), we examined several protein kinases that have been linked to AMPAR plasticity. We began with CaMKII because of its critical role in AMPAR synaptic insertion (Hayashi et al., 2000). In the PSD fraction, the ratio of phosphorylated CaMKII (Thr286) to total CaMKII was significantly increased in the Coc-SA WD45 group compared to the Sal-SA WD45 group; this was observed for both α and β CaMKII isoforms (Fig. 6A; t₁₄=2.1, p<.05; Fig. 6B; t₁₄=2.2, p<.05). The increased ratios in the Coc-SA WD45 group reflected small increases in phosphorylated CaMKII combined with small decreases in total CaMKII (data not shown). We also evaluated CaMKI because it has been implicated in synaptic insertion of CP-AMPARs in the hippocampus (Guire et al., 2008; Fortin et al., 2010), but no differences in CaMKI phosphorylation or total levels were observed between experimental groups (Fig. 6C). Next we examined extracellular signal-regulated kinase (ERK) because its activation also plays a role in AMPAR synaptic incorporation (Zhu et al., 2002). In the PSD fraction, total levels of ERK1 and ERK2 were not significantly altered by cocaine exposure, though there was a trend towards an increase in both isoforms in the Coc-SA WD1 group compared to control (Fig. 7A). Similarly, there was a trend towards an increase in pERK2 in the Coc-SA WD1 group, as well as a smaller trend in the Coc-SA WD45 group (Fig. 7B). The ratio of pERK2/total ERK2 protein was significantly increased in PSD fractions from Coc-SA groups on both WD1 and WD45 compared to Sal-SA groups (Fig. 7C; WD1, t₁₆=1.7, p<.05; WD45, t₁₄=1.8, p<.05) The pERK1 signal in the PSD fraction was too weak to reliably quantify.

Figure 6

Phosphorylation state of CaMKIIα/β and CaMKI in the PSD fraction after 1 or 45 days of withdrawal from self-administration. Data are presented as mean (± SEM) expressed as percent of saline controls at each withdrawal time. A) (more ...)

Figure 7

Levels of ERK1 and ERK2 protein and ERK2 phosphorylation in the PSD fraction after 1 or 45 days of withdrawal from self-administration. Data are presented as mean (± SEM) expressed as percent of saline controls at each withdrawal time. A) Total (more ...)

4. Discussion

In drug-naïve adult rats, NAc synapses contain very low levels of CP-AMPARs (Conrad et al., 2008; Reimers et al., 2010). However, GluA1-containing CP-AMPARs (homomeric GluA1 or GluA1A3 receptors) accumulate in NAc synapses after withdrawal from extended access cocaine self-administration (Conrad et al., 2008). Most importantly, these CP-AMPARs mediate the expression of incubated cue-induced cocaine seeking on WD45. This CP-AMPAR dependent enhancement of drug seeking behavior may be due to an increase in the reactivity of NAc medium spiny neurons to glutamate as a result of the higher conductance of CP-AMPARs compared to the CI-AMPARs that normally dominate in NAc synapses. Incorporation of only a small number of these high conductance CP-AMPARs can significantly increase synaptic strength (Guire et al., 2008).

In humans, it is relatively common for cocaine users to become abstinent for a month or so due to incarceration or hospitalization (Reichel and Bevins, 2009), a period of time that is sufficient for incubation (Lu et al., 2004) and synaptic incorporation of CP-AMPARs in the NAc in rats (present results). If incubation occurs in humans, as indicated by a recent study (Bedi et al., 2010), and if addition of CP-AMPARs also participates in enhanced drug seeking and craving in human cocaine addicts, then blocking or removing CP-AMPARs should help a recovering addict remain abstinent. Unfortunately, the known CP-AMPAR blockers are neurotoxins, so other strategies must be found. To aid in the development of such strategies, it is important to understand where CP-AMPARs are expressed in NAc neurons and what mechanisms enable them to accumulate in these synapses (from which they are largely excluded under normal conditions). We approached this problem by using subcellular fractionation and biotinylation to analyze AMPAR subunit and TARP distribution in the NAc of “incubated rats” on WD30-45.

Subcellular location of CP-AMPARs in the NAc of “incubated rats”

Consistent with prior protein crosslinking results (Conrad et al., 2008), the LP1 and biotinylation studies presented here indicated increased surface expression of GluA1 but not GluA2 in the NAc of Coc-SA rats on WD35, suggesting increased surface expression of CP-AMPARs. Confirming this, electrophysiological studies detected inwardly rectifying AMPAR excitatory postsynaptic currents (EPSCs) on WD30-33. Inward rectification is a hallmark of CP-AMPARs (Cull-Candy et al., 2006). We had previously used electrophysiological approaches to demonstrate the presence of CP-AMPARs on WD42-47, although biochemical results suggested that they were present even earlier in withdrawal (Conrad et al., 2008). In a mouse study, Mameli et al. (2009) detected CP-AMPARs in the NAc shell on WD35 after a similar cocaine self-administration regimen. Because these electrophysiological experiments assess synaptic responses, they establish that at least some of the new CP-AMPARs detected biochemically are located in synapses. However, our results suggest that synaptic CP-AMPARs are more loosely anchored to the PSD than GluA1A2-containing AMPARs, because increased GluA1 levels were detectable in a LP1 fraction from Coc-SA rats on WD45 (i.e., a fraction not exposed to detergent) whereas levels of GluA1 (and γ2) were slightly decreased in a PSD fraction from identically treated rats.

To determine if extrasynaptic CP-AMPAR levels are also increased, we measured GluA1 in a fraction enriched for extrasynaptic membranes. Starting with NAc tissue from adult drug-naïve rats, we showed previously that GluA1-3 subunits are detected in this fraction, albeit at lower levels than in synaptic membranes, and that some extrasynaptic AMPARs are homomeric GluA1 receptors (Ferrario et al., in press). Here we found that GluA1 levels in extrasynaptic membranes were not significantly increased in the Coc-SA group compared to the Sal-SA group on WD45 (although GluA1 phosphorylation in extrasynaptic membranes of the Coc-SA group was significantly increased, as discussed below). It is well established that AMPARs diffuse in the extrasynaptic membrane, and between extrasynaptic and synaptic areas, although their mobility decreases substantially once they enter synapses (e.g., Borgdoff and Choquet, 2002; Heine et al., 2008; Opazo et al., 2010). Thus, our finding of no significant change in total GluA1 in extrasynaptic membranes, but an increase in CP-AMPAR levels in synapses, may indicate more efficient transfer of CP-AMPARs from extrasynaptic to synaptic pools in the Coc-SA group (although their anchoring to the synapse may be relatively weak; see previous paragraph). However, we cannot exclude the possibility that a functionally significant increase in extrasynaptic GluA1 occurred which was below the limit of detection of our biochemical techniques. Total surface levels of GluA1, measured with biotinylation, were increased ~40% above control (Fig. 4). If this increase is split between synaptic and extrasynaptic GluA1 pools, then the magnitude of the change in the extrasynaptic pool might be very small, particularly since GluA1 is more abundant in synaptic membranes than extrasynaptic membranes (Ferrario et al., in press). Indeed, a small trend towards an increase in extrasynaptic GluA1 is apparent in Fig. 5. Ultimately, this issue could be further examined using electrophysiological techniques.

Cocaine alters distribution of TARPs in the NAc

Type I TARPs (γ2, γ3, γ4 and γ8) are auxiliary AMPAR subunits that associate with AMPARs early in their biosynthesis and then regulate both their trafficking and channel function (Ziff, 2007; Kato et al., 2010). γ2 and γ4 are expressed in the NAc, although interestingly γ2 is enriched in synaptic membranes but present in extrasynaptic membranes, whereas γ4 is mainly expressed in extrasynaptic membranes (Ferrario et al., in press). In fact, γ4 cannot be detected in PSD fractions from either drug-naïve rats (Ferrario et al., in press) or Coc-SA rats (present results). The presence of γ2 and γ4 in our NAc samples is consistent with prior studies showing γ2 and γ4 in the NAc and other striatal regions (Klugbauer et al., 2000; Sharp et al., 2001; Tomita et al., 2003; Fukaya et al., 2005). These studies also detected γ3 and γ8 in striatal tissue. However, lack of a selective antibody precluded our investigation of γ3, and γ8 does not appear to be expressed by NAc medium spiny neurons, at least in primary cultures (C.R. Ferrario and M.E. Wolf, unpublished observations). Two subfamilies of Type I TARPS are now recognized: Type Ia (γ2 and γ3) and Type Ib (γ4 and γ8). Both subfamilies regulate AMPAR trafficking, but Type Ib TARPs produce a more dramatic enhancement of AMPAR currents (Kato et al., 2010), suggesting functional consequences of the different distribution of γ2 and γ4 in the NAc (Ferrario et al., in press).

Our results provide the first evidence that TARPs are affected by cocaine exposure. First, indirect evidence from the PSD fraction (see Section 3.3) suggests that the new CP-AMPARs in synapses are associated with γ2. However, the Coc-SA group exhibited a trend towards increased γ2 and especially γ4 in the LP1 fraction, suggesting that some new CP-AMPARs contain γ4. These may be extrasynaptic CP-AMPARs, since very little γ4 is detected in synaptic membranes (see previous paragraph). Furthermore, increased γ4 surface expression was found in biotinylated NAc tissue, which includes the extrasynaptic surface AMPAR pool. Further interpretation of our results is difficult because the stoichiometry of TARPs in AMPAR tetramers remains unclear and may be cell specific, and because it is not clear whether a given AMPAR tetramer can contain more than one TARP isoform (see Tomita et al., 2003; Shi et al., 2009; Kim et al., 2010). Despite these questions, given that TARP phosphorylation state regulates AMPAR synaptic targeting (see next section), it is possible that cocaine influences AMPAR trafficking in part through mechanisms that involve TARPs.

Role of GluA1 phosphorylation in CP-AMPAR accumulation

GluA1 phosphorylation is regulated in concert with synaptic plasticity and influences both channel function and receptor trafficking (Derkach et al., 2007; Shepherd and Huganir, 2007; Lin et al., 2009; Lu et al., 2010). We focused on S845, which is phosphorylated by protein kinase A (Roche et al., 1996; Mammen et al., 1997). Phosphorylation of S845 can increase AMPAR open probability (Banke et al., 2000) although the majority of recent studies have focused on its role in AMPAR trafficking. In NAc neurons, we have shown that GluA1-containing AMPARs are incorporated into synapses via a two-step process. They are first inserted onto the cell surface at extrasynaptic sites, and then translocated into synapses in an NMDA receptor-dependent manner. The first step is accelerated by PKA activation, most likely via phosphorylation of S845 in GluA1 (Chao et al., 2002a,b; Mangiavacchi and Wolf, 2004; Sun et al., 2008; for review, see Wolf, 2010b). Studies in other brain regions similarly indicate that PKA phosphorylation of GluA1 primes AMPARs for synaptic insertion (Esteban et al., 2003; Sun et al., 2005; Oh et al., 2006; Gao et al., 2006; Man et al., 2007) and that extrasynaptic surface AMPARs are important for supplying the synapse (Passafaro et al., 2001; Sun et al., 2005; Gao et al., 2006; Yudowski et al., 2007; Heine et al., 2008; Yang et al., 2008; Lin et al., 2009; Makino and Malinow, 2009). Particularly relevant to our study, perisynaptic CP-AMPARs are important for increasing synaptic strength during some types of LTP (Guire et al., 2008; Yang et al., 2010).

Recently, we found that pS845 GluA1 is enriched in extrasynaptic membranes (relative to synaptic membranes) from the NAc of adult drug-naïve rats (Ferrario et al., in press). In the present study, we observed an increase in the pS845 GluA1/total GluA1 ratio in the extrasynaptic membrane fraction from the Coc-SA WD45 group compared to Sal-SA controls. We also observed increased pS845 GluA1 on the cell surface in the Coc-SA WD35 group (biotinylation experiments). These results suggest that a greater portion of the extrasynaptic GluA1 is phosphorylated at S845 in “incubated rats”. In light of evidence that S845 phosphorylation primes GluA1-containing AMPARs for synaptic insertion (above), the observed increase in extrasynaptic pS845 GluA1 could play a causal role in the elevation of synaptic CP-AMPAR levels that we observe in electrophysiological studies on WD30-47. Interestingly, a recent study showed that S845 phosphorylation stabilizes perisynaptic GluA1-containing CP-AMPARs in hippocampus, preventing their degradation (He et al., 2009). However, this would predict an increase in total GluA1 protein in the extrasynaptic pool, which we did not observe, although a small effect cannot be ruled out (see second section of Discussion). An increase in S845 GluA1, in concert with addition of GluA1-containing CP-AMPARs to hippocampal synapses, has also been reported 12 h after discontinuing repeated morphine injections (Billa et al., 2010).

Candidate signaling mechanisms for CP-AMPAR plasticity during incubation

We studied signaling pathway activation in PSD fractions from Coc-SA rats to learn about mechanisms that may underlie CP-AMPAR accumulation. CaMKII was studied because its activation is required for activity-dependent synaptic insertion of GluA1-containing AMPARs although GluA1 is not the relevant substrate (Hayashi et al., 2000). Instead, recent evidence indicates that activity-dependent translocation of CaMKII to the PSD leads to phosphorylation of γ2, facilitating γ2 binding to PSD-95; this promotes synaptic retention of γ2 and associated GluA1-containing receptors (Opazo et al., 2010). Phosphorylation of γ2 may promote its binding to PSD-95 by dissociating γ2 from lipid bilayers; the lipid interaction inhibits γ2 binding to PSD-95 (Sumioka et al., 2010). Given that we observed an increase in pCaMKII/total CaMKII in the PSD fraction of the Coc-SA WD45 group, it is possible that the mechanism identified by Opazo et al. (2010), activated persistently, could underlie our observations of higher levels of GluA1-containing AMPARs in the LP1 fraction and on the cell surface in the biotinylation experiments (γ2 and γ4 showed trends towards increases in the LP1 fraction, although only surface γ4 increased in the biotinylation experiment). It would be interesting to determine if TARP phosphorylation is increased in the NAc of “incubated rats”, but we have been unable to resolve different TARP phosphorylation states in NAc tissue using electrophoresis or other methods and no phospho-specific antibodies are available. It is also possible that the increase in CaMKII activation in the Coc-SA group is a consequence of the presence of CP-AMPARs in the membrane, as they would provide a new source of Ca²⁺ entry that could lead to CaMKII activation.

Prior work has implicated CaMKII in cellular and behavioral changes involving the NAc that are produced by repeated psychostimulant exposure, but this work has been conducted after non-contingent drug treatment (e.g., Pierce and Kalivas, 1997; Pierce et al., 1998; Boudreau et al., 2009; Loweth et al., 2010) or limited access drug self-administration (e.g., Anderson et al., 2008; Loweth et al., 2008; Wang et al., 2010). Interestingly, in the latter experimental situation, cocaine-primed reinstatement was shown to require a rapid CaMKII dependent increase in cell surface GluA1 associated with GluA1 phosphorylation at S831 (Anderson et al., 2008). However the time course of this effect (AMPAR changes were detected 30 min after the priming injection of cocaine) is markedly different from the extremely persistent increases in GluA1 surface expression (weeks to months) that we are studying in the incubation model. Somewhat more analogous are studies showing that over-expression of CaMKII for several days led to an increase in AMPAR-mediated responses in the NAc (Loweth et al., 2010; Singer et al., 2010), although the time-scale is still quite different.

When comparing our results to studies using other cocaine self-administration procedures, it is important to recognize that we do not know whether CP-AMPAR levels increase in the NAc after prolonged withdrawal from limited access cocaine self-administration (see Wolf and Ferrario, 2010 for more discussion). All of the evidence for CP-AMPAR accumulation has been obtained after withdrawal from extended access cocaine self-administration (Conrad et al., 2008; Mameli et al., 2009; present study). Extended access self-administration produces different behaviors compared to limited access cocaine self-administration, suggesting that the two experiences do not produce equivalent neuroadaptations. Based on the behavioral differences, extended access is believed to more closely model the compulsive drug seeking and taking characteristic of addiction than limited access procedures (Ahmed and Koob, 1998; Vanderschuren and Everitt, 2004; Ferrario et al., 2005; Ben-Shahar et al., 2009). It will be interesting to test for CP-AMPARs after prolonged withdrawal from very long duration cocaine self-administration regimens (8–10 weeks); striking individual differences in NAc synaptic plasticity are evident 24 h after discontinuing such a regimen (Kasanetz et al., 2010).

We also evaluated CaMKI based on evidence that it mediates redistribution of CP-AMPARs from extrasynaptic pools to the synapse during theta-burst stimulation (TBS) induced LTP (Guire et al., 2008) as well as the spine enlargement that accompanies AMPAR synaptic insertion (Fortin et al., 2010). However, we found no evidence for CaMKI activation in the PSD fraction (or LP1; data not shown) of Coc-SA rats. This is not surprising in light of differences between the two situations. Hippocampal LTP is a rapidly occurring form of synaptic strengthening elicited by a relatively discrete stimulus. With some paradigms (e.g., TBS), the initial synaptic strengthening depends on synaptic incorporation of CP-AMPARs but these are replaced within 20–30 min by CI-AMPARs (Plant et al., 2006; Guire et al., 2008). In contrast, in the incubation model, CP-AMPARs are added to NAc synapses over weeks (Conrad et al., 2008), and then remain in synapses for at least 69 days (K.Y. Tseng, unpublished findings). At least from the perspective of timing of onset, this is more reminiscent of homeostatic plasticity than Hebbian plasticity (see Sun and Wolf, 2009 for more discussion) and it is therefore not surprising that different mechanisms underlie CP-AMPAR plasticity in these two situations. The time course of our effect also contrasts with studies showing CP-AMPAR synaptic incorporation in other brain regions during the first day after discontinuing cocaine, morphine or benzodiazepine exposure (e.g., Bellone and Lüscher, 2006; Song et al., 2007; Argilli et al., 2008; Billa et al., 2010; Good and Lupica, 2010).

ERK2 activation is involved in many aspects of addiction-related plasticity (Lu et al., 2006; Girault et al., 2007) and prior studies have demonstrated that ERK2 activation accompanies increased cell surface levels of GluA1A2-containing AMPARs in the NAc of rats treated repeatedly with non-contingent cocaine to produce behavioral sensitization (Boudreau et al., 2007, 2009; Schumann and Yaka, 2009). Here, we found that the ratio of pERK2/total ERK2 was significantly increased in the NAc PSD fraction from Coc-SA rats on both WD1 and WD45. This duration of ERK2 activation is remarkable, although we had previously found ERK2 activation lasting at least 2 weeks in the NAc of cocaine-sensitized rats (Boudreau et al., 2009). It is possible that ERK2 activation plays a role in maintaining GluA1-containing AMPAR upregulation after withdrawal from either non-contingent or contingent cocaine exposure. Interestingly, the correlation between upregulation of GluA1-containing AMPARs in the NAc and ERK2 activation is bidirectional: both AMPAR subunit surface expression and pERK2 increase 24 after withdrawal, whereas subsequent re-exposure to cocaine normalizes both measures (Boudreau et al., 2007).

Conclusions

CP-AMPARs are increased in NAc synapses of rats evaluated after withdrawal from extended access cocaine self-administration (“incubated rats”). This is detected as early as WD30-33 and persists at least through WD69 (and perhaps beyond), making it among the more persistent drug-induced neuroadaptations that have been described. Our results suggest that new CP-AMPARs in NAc synapses are loosely tethered to the PSD and may be associated with γ2. Increased synaptic levels of CP-AMPARs may result in part from an increase in S845 phosphorylation of GluA1 in extrasynaptic membranes, given that S845 phosphorylation primes GluA1-containing AMPARs for synaptic insertion and extrasynaptic AMPARs supply the synapse. Some of the new extrasynaptic CP-AMPARs are likely associated with γ4, rather than γ2. The maintenance of CP-AMPARs in synapses across more than a month of withdrawal is accompanied by activation of ERK2; furthermore, CaMKII but not CaMKI is activated on WD45. Future studies should explore a role for additional signaling pathways, and examine how these postsynaptic adaptations may be related to changes in presynaptic glutamate tone (see Wolf, 2010a).

Acknowledgements

These studies were supported by DA015835, DA09621, DA000453 and DA029099 to M.E.W., and Rosalind Franklin University Start-up funds and DA004093 to K.Y.T. C.R.F. was supported by postdoctoral National Research Service Award DA024502. We thank Dr. Naohito Nozaki (Department of Biochemistry and Molecular Biology, Kanagawa Dental College, Kanagawa, Japan) for generously providing CaMKI antibodies, Dr. Susumu Tomita (Yale University, New Haven, CT) for helpful discussions and Dr. Michela Marinelli for the use of her equipment.

Abbreviations


aCSF	artificial cerebral spinal fluid

BS³	bis(sulfosuccinimidyl)suberate

CaMK	Ca²⁺/calmodulin-dependent protein kinase

CI-AMPAR and CP-AMPAR	Ca²⁺-impermeable and Ca²⁺-permeable AMPAR

DTT	dithiothreitol

EPSC	excitatory postsynaptic current

ERK	extracellular signal-regulated kinase

pS845 GluR1	GluA1 phosphorylated at serine 845

LP1	lysed synaptosomal membranes

NAc	nucleus accumbens

PSD	postsynaptic density

TARP	transmembrane AMPA receptor regulatory protein

WD	withdrawal day

Footnotes

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Disclosure

M.E.W. has no biomedical financial interests but has a patent on A Possible Therapy For Cue-Induced Cocaine Craving Leading to Relapse in Abstinent Cocaine Abusers Based on Blockade of GluR2-lacking AMPA Receptors in the Nucleus Accumbens. The other authors report no biomedical financial interests or potential conflicts of interest.

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