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Logo of joncJournal of Oncology
J Oncol. 2011; 2011: 950140.
Published online 2011 March 28. doi:  10.1155/2011/950140
PMCID: PMC3090638

Hypermethylation of CCND2 May Reflect a Smoking-Induced Precancerous Change in the Lung


It remains unknown whether tobacco smoke induces DNA hypermethylation as an early event in carcinogenesis or as a late event, specific to overt cancer tissue. Using MethyLight assays, we analyzed 316 lung tissue samples from 151 cancer-free subjects (121 ever-smokers and 30 never-smokers) for hypermethylation of 19 genes previously observed to be hypermethylated in nonsmall cell lung cancers. Only APC (39%), CCND2 (21%), CDH1 (7%), and RARB (4%) were hypermethylated in >2% of these cancer-free subjects. CCND2 was hypermethylated more frequently in ever-smokers (26%) than in never-smokers (3%). CCND2 hypermethylation was also associated with increased age and upper lobe sample location. APC was frequently hypermethylated in both ever-smokers (41%) and never-smokers (30%). BVES, CDH13, CDKN2A (p16), CDKN2B, DAPK1, IGFBP3, IGSF4, KCNH5, KCNH8, MGMT, OPCML, PCSK6, RASSF1, RUNX, and TMS1 were rarely hypermethylated (<2%) in all subjects. Hypermethylation of CCND2 may reflect a smoking-induced precancerous change in the lung.

1. Introduction

Lung cancer causes more deaths in the United States each year than breast, colon, pancreatic, and prostate cancer combined, approximately 157,300 deaths estimated in 2010 [1]. Cigarette smoking is the most significant risk factor for developing lung cancer and contributes to 80–90% of these deaths [2, 3].

Over the past four to five decades, significant progress has been made to elucidate the carcinogenic mechanisms of tobacco smoking. Using animal models, it has been shown that among over 60 established carcinogens in cigarette smoke, 20 can cause lung tumors [4]. It has been proposed that these carcinogens, when metabolized, form DNA-adducts which may directly cause genetic alterations if not repaired. When these genetic alterations affect tumor suppressor genes or tumor oncogenes, they can promote cell proliferation and malignant transformation [5]. Studies in lung cancer patients clearly suggest that cigarette smoking can lead to acquisition of genetic mutations in p53 and ras oncogene [6, 7]. In addition, cigarette smoke is proposed to cause immunosuppression, which provides an environment for tumor progression [8, 9].

Recently, DNA hypermethylation has been recognized as an alternative, epigenetic mechanism for gene silencing in lung cancer, in addition to genetic mutation. Several environmental exposures are thought to cause aberrant DNA methylation, including dietary factors, chemotherapeutic agents, and heavy metals [10]. Tobacco smoke exposure has been associated with increased expression of DNA methyltransferases [1114]. Consistent with this observation, lung cancers arising in heavy smokers show increased hypermethylation of various genes, especially CDKN2A (p16) and RASSF1, compared with lighter smokers or nonsmokers [1527].

However, these results do not reveal whether DNA hypermethylation occurs early or late in carcinogenesis. Early changes in carcinogenesis (especially those related to smoking) are hypothesized to occur somewhat diffusely in the lung and may therefore be detectable in noncancerous lung tissue, as well as in any cancers which arise [2830]. For example, frequent hypermethylation of CDKN2A, RASSF1, CDH13, and other genes has been observed in sputum samples from cancer-free smokers, suggesting that they may be hypermethylated early [3134]. In contrast, late changes in carcinogenesis are thought to arise mainly in overtly malignant tissues.

We recently analyzed matched cancerous and noncancerous lung tissues from patients with nonsmall cell lung cancer (NSCLC). We observed that in the 27 genes tested, most DNA methylation changes were tumor-specific and therefore might be considered late changes in carcinogenesis [35]. However, in these NSCLC patients, a small number of genes, including CCND2, APC, CDH1, and RARB (Table 1), were also hypermethylated in a portion of noncancerous lung tissues, suggesting that one or more of these genes might become hypermethylated as an early precancerous change. We hypothesized that early changes in DNA methylation, if present, might be associated with exposure to cigarette smoke. Furthermore, because smoking-related lung tumors and emphysema are known to disproportionately affect the upper lobes of the lungs [36, 37], we hypothesized that methylation changes related to smoking would similarly be more frequent in the upper lobes, compared with the lower lobes.

Table 1
Genes hypermethylated in >2% of noncancerous lung tissues.

2. Materials and Methods

2.1. Subject Enrollment

All procedures were conducted in accordance with institutional review board and human subjects committee approval. Subjects were retrospectively enrolled who had undergone lung surgeries (lung volume reduction, lung transplant, wedge biopsy, or lobectomy) for nonmalignant diseases including emphysema, chronic bronchitis, bronchiectasis, granulomatous disease, various infectious diseases, and cystic or pulmonary fibrosis, at the University of Washington Medical Center (UWMC) between July 1st 1995 and July 1st 2005. All specimens were reviewed by an expert pathologist (CDJ) to confirm that they represented noncancerous lung tissue. All clinical data were gathered from subjects' UWMC medical records, including smoking history and primary pulmonary diagnosis. Subjects were excluded for the following reasons: previous diagnosis of lung cancer, insufficient lung tissue for methylation analysis, or unknown pack years of smoking. In total, 372 nonmalignant lung tissue samples from 159 subjects were identified for DNA methylation analysis.

2.2. DNA Isolation from Paraffin Blocks

From each block, six 20-μm sections were cut and deparaffined by xylene extraction. Proteinase K was used to digest the resulting tissue pellets overnight, at 48°C. Genomic DNA was then isolated by phenol/chloroform extraction and ethanol precipitation. Finally, DNA was purified using a QIAamp DNA minicolumn (Qiagen) according to the manufacturer's instructions.

2.3. Sodium Bisulfite Conversion

As previously described in detail [42], in vitro fully methylated DNA (methylated DNA control) and human sperm DNA (unmethylated DNA control) were converted with clinical samples. Briefly, ~1 μg DNA was modified by 5 mol/L sodium bisulfite, desulfonated with NaOH, and then purified and resuspended in 80 μL elution buffer (EB; 10 mmol/L Tris-HCl, pH 8.0).

2.4. DNA Methylation (MethyLight) Assay

All primers and probes for MethyLight assays were designed specifically for bisulfite-converted fully methylated DNA. Their sequences have been reported previously [35]. Amplification of bisulfite converted beta-actin (ACTB) DNA was used to normalize for the quantity of input DNA. Samples negative for ACTB were excluded from methylation analysis. Of 372 identified samples, 56 (15%) were excluded because they were negative for ACTB. The percentage of samples excluded after bisulfite conversion was similar in smokers (15%) and nonsmokers (16%). A plasmid containing bisulfite converted ACTB gene of known concentration was diluted and used as a standard curve for quantification. The assay for a given set of samples was only considered valid if the converted unmethylated human sperm DNA was not amplified, whereas the converted fully methylated DNA was amplified. For each locus, the percentage methylated reference (PMR) was calculated by dividing the gene/reference ratio of a sample by the gene/reference ratio of fully methylated DNA control [43]. Genes were considered to be positive for any hypermethylation at PMR >0%.

2.5. Statistical Methods

For comparisons between groups, to provide independent observations, we randomly selected one tissue block per subject to represent each subject's hypermethylation profile. To evaluate potential differences in gene methylation by site of the lung, paired upper, and lower lobe tissue samples from within subjects were compared using McNemar's Test. To assess the univariate and multivariate relationships between gene methylation and independent variables (smoking, age, gender, lobe of lung, pack years, and years since quitting), we included all available tissue samples from each subject and employed generalized estimating equations (GEE). This method enables the analysis of data with repeated measurements (multiple tissue samples per subject from different lobes) and accounts for within-subject correlations. In selecting a model, a logit link was used and we assumed an exchangeable working correlation structure to account for intrasubject correlation. Parameter estimates were exponentiated to provide odds ratios (OR) and 95% confidence intervals (CI). A 2-sided 0.05 test level determined statistical significance for all analyses. All analyses were conducted using SAS version 9.1 (SAS Institute Inc., Cary, NC).

3. Results

3.1. Study Population and Tissue Samples

We retrospectively enrolled 151 subjects who contributed a total of 316 available pathology blocks (Table 2). At the time of their surgery, 121 subjects were current or former smokers (ever-smokers), while 30 reported no smoking history (never-smokers). Among the never-smokers, none had any history of cancer, either prior to or after the surgery that yielded the tissue used in this study. Of the ever-smokers, 10 had a history of prior cancer other than lung (1 breast, 2 cervical, 2 colon, 2 prostate, 1 testicular, and 2 uterine) and all were cancer free at surgery. Four of the ever-smokers developed a cancer subsequent to the surgery that yielded the cancer-free lung tissue (1 bladder, 1 colon, and 2 NSCLCs, one at 2 years after, one at 5 years after). The clinical data show that never-smokers and ever-smokers who had undergone lung surgery comprised two distinct populations. Ever-smokers were significantly older than never-smokers (61 years versus 44 years). Further, of ever-smokers who contributed specimens from lung surgery, 71% had a diagnosis of emphysema, compared to only 10% of never-smokers.

Table 2
Clinical data of 151 cancer-free subjects with lung tissue available for MethyLight assay.

We analyzed a total of 316 available pathology blocks from these 151 subjects, including 177 upper lobe samples, 105 lower lobe samples, 30 middle lobe or lingula samples, and 4 whose lobe of origin was unclear. Multiple blocks were available for 98 (81%) of ever-smokers and 13 (43%) of never-smokers; from the 121 ever-smokers, 269 samples were tested, while from the 30 never-smokers, 47 samples were tested. Sample sites varied substantially in ever-smokers and never-smokers as 50% of ever-smokers, compared to 17% of never-smokers, contributed only samples from the upper lobes. This difference arose because many ever-smokers in our sample underwent lung volume reduction surgery for emphysema, which predominantly affects the upper lung zones when induced by smoking.

3.2. Gene Hypermethylation and Smoking Status

Considering one random tissue block per subject, only APC (39%), CCND2 (21%), CDH1 (7%), and RARB (4%) were hypermethylated in more than 2% of subjects (Figure 1). All 15 remaining genes (BVES, CDH13, CDKN2A (p16), CDKN2B, DAPK1, IGFBP3, IGSF4, KCNH5, KCNH8, MGMT, OPCML, PCSK6, RASSF1, RUNX, and TMS1) were hypermethylated in less than 2% of subjects. CCND2 was hypermethylated significantly more frequently in ever-smokers compared to never-smokers (26% versus 3%, P < .001). APC was hypermethylated somewhat more frequently in ever-smokers (41% versus 30%), but this did not achieve statistical significance (P = .3).

Figure 1
Hypermethylation of four genes in noncancerous lung tissues. Percent of subjects with hypermethylation of four genes (APC, CCND2, CDH1, and RARB), stratified by smoking status. Samples were considered to be positive for any hypermethylation at PMR >0%. ...

3.3. Correlation of APC and CCND2 Gene Hypermethlation

APC and CCND2 were often hypermethylated in the same samples; 179 (57%) samples were negative for both genes, 16 (5%) were positive for hypermethylation of CCND2, but not APC, 68 (22%) were positive for hypermethylation of APC but not CCND2, and 53 (17%) samples were positive for both genes. CCND2 hypermethylation was significantly correlated with APC hypermethylation in all subjects (OR = 7.3, 95% CI = 3.9–13.8) and in smokers only (OR = 7.4, 95% CI = 3.9–14.0). In nonsmokers, 31 (66%) samples were negative for both genes, 14 (30%) were positive for APC only, and 2 (4%) samples were positive for both APC and CCND2.

3.4. Gene Hypermethylation and Clinical and Demographic Factors

In univariate GEE analyses of all specimens (Table 3), CCND2 hypermethylation was significantly associated with a positive smoking history, increasing age, and sample origin from the upper versus lower lobe of the lung. APC hypermethylation was significantly less frequent among females and moderately more frequent in upper lobes compared to lower lobes but was not significantly associated with a positive smoking history. In a multivariate model simultaneously assessing smoking history, age, gender, and location of the sample (upper versus lower lobe) in all subjects, hypermethylation of CCND2 remained significantly associated with increased age (OR = 1.7, 95% CI = 1.2–2.4 for each 10 years of age) and upper lobe location (OR = 2.0, 95% CI = 1.0–3.8). CCND2 hypermethylation was somewhat associated with a positive smoking history (OR = 2.8, 95% CI = 0.6–12.1) but this did not achieve statistical significance.

Table 3
All subjects—odds ratios for promoter hypermethylation (95% CI).

3.5. Gene Hypermethylation and Duration of Smoke Exposure

Within the subset of 269 samples from 121 ever-smokers (Table 4), APC hypermethylation was not related to pack-years of cigarette smoking or years since quitting smoking. In univariate GEE analysis, CCND2 hypermethylation was significantly associated with greater pack years but was not related to years since quitting. However, in a multivariate GEE analysis simultaneously assessing pack years, years since quitting, age, gender, and location of the sample (upper versus lower lobe), CCND2 hypermethylation was no longer associated with pack years (OR = 1.0, 95% CI = 0.9–1.2 per 10 pack years).

Table 4
Ever-smokers only—odds ratios for promoter hypermethylation (95% CI).

3.6. Gene Hypermethylation in Upper- and Lower-Lobe Samples

Smoking-related lung tumors and emphysema are known to disproportionately affect the upper lobes of the lungs. Thus, if hypermethylation of a gene is associated with smoking, we might expect to find more hypermethylation in upper lobe samples compared to lower lobe samples, among ever-smokers.

Examining all 269 samples from ever-smokers (Table 4), in univariate GEE analysis, both APC (OR = 2.0, 95% CI = 1.1–3.5) and CCND2 (OR = 1.9, 95% CI = 1.0–3.5) hypermethylation were more common in upper compared to lower lobes. In multivariate analysis including pack-years, years since quitting, age, gender, and upper versus lower lobe, APC hypermethylation remained significantly associated with upper-lobe sample location (OR = 2.1, 95% CI = 1.1–4.0), while CCND2's positive association with upper lobes was reduced to slightly below the level of statistical significance (OR = 1.7, 95% CI = 0.9–3.4).

Among the 121 ever-smokers in our cohort, 30 had both upper and lower lobe samples available, and were included in within-subjects, pairwise comparisons. For APC, 12 of 30 pairs had discordant hypermethylation status (1 positive and 1 negative), of which 8 of 12 displayed APC hypermethylation in an upper lobe but not a lower lobe sample (P = .25). For CCND2, only 7 of 30 pairs had discordant hypermethylation status, of which only 3 of 7 were hypermethylated in the upper but not the lower lobe (P = .7). Thus, too few subjects had discordant hypermethylation in upper and lower lobe samples to yield statistically meaningful results in within-subjects comparisons.

4. Discussion

DNA hypermethylation is an important event in lung carcinogenesis. However, it is currently unknown whether changes in DNA methylation are early events, occurring in previously normal lung tissue or whether they are late changes that occur only in overt tumor cells [30]. To attempt to answer these questions, we tested DNA hypermethylation in lung tissues from subjects without cancer—both smokers and nonsmokers—using a panel of 19 genes which we had previously found to be hypermethylated in some nonsmall cell lung cancers [35, 44]. This unique study design allowed us, for the first time, to characterize the DNA hypermethylation profile of nonsmokers' lung tissues and to compare this profile to that of smoke-exposed lung.

Importantly, we observed that CCND2, which is known to be frequently hypermethylated in lung cancer tissue [35, 4447], was hypermethylated more frequently in ever-smokers (26%) than in never-smokers (3%). Also, as predicted, in ever-smokers, CCND2 was hypermethylated more frequently in samples from the upper lobes, which are known to suffer far more negative effects from cigarette smoke, such as lung cancer and emphysema [36, 37]. These findings support the conclusion that CCND2 reflects an early, precancerous change in the lung, caused by cigarette smoke.

CCND2 encodes cyclin D2, a protein involved in cell cycle progression that is thought to act as a regulator of cyclin dependent kinase 4 and cyclin dependent kinase 6 in the transition from G1 to S-phase [39]. CCND2 hypermethylation appears to be common in many cancers. In breast cancer, where it has been studied most extensively, CCND2 hypermethylation is detected frequently, though it appears to be rarely detected in normal breast tissue [4854]. Interestingly, while CCND2 hypermethylation (and therefore low CCND2 protein expression) has been associated with poor prognosis in epithelial ovarian cell cancer [55] and recurrence of hepatocellular carcinoma [56], increased CCND2 expression has been associated with poor prognosis in diffuse large B-cell lymphoma [57].

In the lung, CCND2 hypermethylation has been found in 40–56% of NSCLCs [35, 44, 45, 47]. In noncancerous lung tissue, whereas Virmani et al. found CCND2 hypermethylation in 0 of 18 samples [45], our previous investigation found CCND2 hypermethylation in 24% of noncancerous lung tissues from patients with NSCLC [35]. This closely matches the rate observed in the present study, in cancer-free ever-smokers (26%). Possibly, our group observed a higher rate of CCND2 hypermethylation in both cancerous and cancer-free lung tissues because we used MethyLight assays instead of methylation-specific PCR (MSP), which was used by Virmani et al. Thus, we may have detected low levels of hypermethylated genes in cancer-free tissues which were not detected by MSP. Discrepancies may also be due to the somewhat different primers and probes used in analyses, which indicate different sequence regions investigated. In addition, Kubo et al. did not observe any CCND2 hypermethylation in 30 matched noncancerous lung tissues but it should be noted that in this study, 70% of subjects were nonsmokers who would not be expected to have significant rates of CCND2 hypermethylation [46].

Combined, these results reveal a progression in the rate of CCND2 hypermethylation in the lung, corresponding with the risk for developing lung cancer. While CCND2 hypermethylation was very infrequent (3%) in our current study's low-risk group of 30 never-smokers, it was more frequent in a high-risk group of ever-smokers (24–26% in our current and previous studies), and most frequent in overt NSCLC tissue (40–56%). This risk-stratified progression in lung tissues suggests that CCND2 hypermethylation may truly reflect an early precancerous change in the lung, en route to overt cancer, which may be due to the effects of smoking.

Still, our findings regarding CCND2 should be regarded as preliminary at this time, for several reasons. In multivariate analysis, the effect of smoking status on CCND2 hypermethylation was reduced to trend-level significance after taking into account the effects of sample location (upper versus lower lobe) and subject age. This likely occurred because in our sample, the majority of smokers underwent lung surgery for emphysema and represented a significantly older group, more likely to contribute samples from upper lobes (where emphysema is most prominent). In contrast, nonsmokers were younger and underwent lung resection for a variety of diseases. With such significant correlation of these factors, multivariate analysis may not have reliably separated each factor's relative contribution to gene hypermethylation. Thus, observed differences in the rate of CCND2 hypermethylation could be attributable to any of these factors or others that differed between ever and never-smokers. Emphysema, for example, made CCND2 hypermethylation more likely although significant rates of CCND2 hypermethylation were also found in smokers with other diagnoses. While CCND2 hypermethylation could be part of the unique pathophysiology of emphysema, it more likely arose because emphysema reflects severe smoking-induced lung damage. The effect of age on CCND2 hypermethylation has not been studied previously in noncancerous lung, although several genes have been reported to undergo increased rates of hypermethylation with age, in various body tissues, including CDH1 and DAPK1 in the lung [58]. In noncancerous breast epithelium [59] and in peripheral blood samples from cancer-free subjects [60], advanced age was not observed to correlate with CCND2 hypermethylation. Thus, the relationship between age and CCND2 hypermethylation remains unknown at this time. In weighing the relative contributions of age, sample location, and emphysema status on CCND2 hypermethylation, it is worth noting that smoking history was by far the strongest single predictor of CCND2 hypermethylation in univariate analysis (OR = 6.9, 95% CI = 1.6–29.8). One limitation of the present study was that despite our overall large number of 151 subjects, only 30 were never-smokers. This occurred because never-smokers far less frequently undergo lung resections which produce tissue. This may have been part of the reason why in multivariate analysis, we observed only trend-level significance for smoking's effect on CCND2 hypermethylation. We were able to improve our statistical power somewhat by using generalized estimating equations (GEE) for our univariate and multivariate analyses, allowing us to enter multiple tissue blocks per subject when available (multiple observations), without biasing the results. However, future studies should seek to verify the low rate of CCND2 hypermethylation we observed in never-smokers. An additional limitation of our study design was that all subjects had an underlying non-cancer pulmonary diagnosis that necessitated lung surgery. Thus, while observed gene hypermethylation was unrelated to cancer, it cannot definitely be said to represent healthy lung. Finally, due to our study design, we only provide indirect evidence of interaction between smoking and CCND2 hypermethylation. Future studies utilizing animal models may be useful to elucidate the potential causal relationship between smoking and CCND2 hypermethylation.

In our current and previous studies, CDKN2A (p16) was hypermethylated in 26% of cancer tissues [44] but was rarely hypermethylated in noncancerous lung tissues, regardless of smoking status [35]. However, CDKN2A hypermethylation has previously been characterized as an early event in lung carcinogenesis [2830], and hypermethylation of CDKN2A has been commonly detected in sputum samples from heavy smokers without lung cancer [32, 61]. Overall, a very wide range of hypermethylation rates for CDKN2A has been reported in the literature, for noncancerous lung tissues. Along with other researchers who observed low rates of CDKN2A hypermethylation in noncancerous lung tissues, our results suggest that CDKN2A hypermethylation may actually represent a later change in carcinogenesis [6266]. However, the surprisingly large discrepancies between studies may be related to differences in assay methodology (including PCR primers and specific CpG islands) or patient populations.

5. Conclusions

CCND2 hypermethylation likely represents an early, smoking-induced, precancerous change in the lung; it is very infrequent in the lung tissue of never-smokers, more frequent among smokers, and most frequent in overt NSCLC tissue. This conclusion should be verified in future investigations. In addition, this study supports the conclusions of our previous investigation, that although they are hypermethylated in many NSCLC tumor tissues, RASSF1, DAPK1, BVES, CDH13, MGMT, KCNH5, and to some extent CDH1 and RARB, are rarely hypermethylated in the cancer-free lung, even after significant tobacco exposure [35]. These genes may therefore yield clues to understanding the later stages of carcinogenesis. In addition, if hypermethylation of CCND2 or other genes represents an early precancerous change, it is possible that drugs aimed at reversing DNA methylation could be used to prevent smoking-related carcinogenesis.

Conflict of Interests

None Declared.


This study was supported by NIH/National Cancer Institute Grants nos. R01 CA115559, R01 CA107264, and R01 CA80907.


1. Cancer Facts & Figures. American Cancer Society, Atlanta, Ga, USA, 2010.
2. CDC. The 2004 United States surgeon general's report: the health consequences of smoking. New South Wales Public Health Bulletin. 2004;15(5-6):p. 107. [PubMed]
3. CDC. Annual smoking-attributable mortality, years of potential life lost, and productivity losses—United States, 1997–2001. Morbidity and Mortality Weekly Report . 2005;54(25):625–628. [PubMed]
4. Hecht SS. Tobacco smoke carcinogens and lung cancer. Journal of the National Cancer Institute. 1999;91(14):1194–1210. [PubMed]
5. Straif K, Baan R, Grosse Y, et al. Carcinogenicity of polycyclic aromatic hydrocarbons. The Lancet Oncology. 2005;6(12):931–932. [PubMed]
6. Ross JA, Nesnow S. Polycyclic aromatic hydrocarbons: correlations between DNA adducts and ras oncogene mutations. Mutation Research. 1999;424(1-2):155–166. [PubMed]
7. Yu D, Berlin JA, Penning TM, Field J. Reactive oxygen species generated by PAH o-quinones cause change-in-function mutations in p53. Chemical Research in Toxicology. 2002;15(6):832–842. [PubMed]
8. Frazer-Abel AA, Baksh S, Fosmire SP, et al. Nicotine activates nuclear factor of activated T cells c2 (NFATc2) and prevents cell cycle entry in T cells. Journal of Pharmacology and Experimental Therapeutics. 2004;311(2):758–769. [PubMed]
9. Kalra R, Singh SP, Savage SM, Finch GL, Sopori ML. Effects of cigarette smoke on immune response: chronic exposure to cigarette smoke impairs antigen-mediated signaling in T cells and depletes IP3-sensitive Ca2+ stores. Journal of Pharmacology and Experimental Therapeutics. 2000;293(1):166–171. [PubMed]
10. Moore LE, Huang WY, Chung J, Hayes RB. Epidemiologic considerations to assess altered DNA methylation from environmental exposures in cancer. Annals of the New York Academy of Sciences. 2003;983:181–196. [PubMed]
11. Lin RK, Hsu HS, Chang JW, Chen CY, Chen JT, Wang YC. Alteration of DNA methyltransferases contributes to 5′CpG methylation and poor prognosis in lung cancer. Lung Cancer. 2007;55(2):205–213. [PubMed]
12. Kwon YM, Park JH, Kim H, et al. Different susceptibility of increased DNMT1 expression by exposure to tobacco smoke according to histology in primary non-small cell lung cancer. Journal of Cancer Research and Clinical Oncology. 2007;133(4):219–226. [PubMed]
13. Liu H, Zhou Y, Boggs SE, Belinsky SA, Liu J. Cigarette smoke induces demethylation of prometastatic oncogene synuclein-gamma in lung cancer cells by downregulation of DNMT3B. Oncogene. 2007;26(40):5900–5910. [PubMed]
14. Belinsky SA, Klinge DM, Stidley CA, et al. Inhibition of DNA methylation and histone deacetylation prevents murine lung cancer. Cancer Research. 2003;63(21):7089–7093. [PubMed]
15. Kim H, Young MIK, Jin SK, et al. Tumor-specific methylation in bronchial lavage for the early detection of non-small-cell lung cancer. Journal of Clinical Oncology. 2004;22(12):2363–2370. [PubMed]
16. Toyooka S, Maruyama R, Toyooka KO, et al. Smoke exposure, histologic type and geography-related differences in the methylation profiles of non-small cell lung cancer. International Journal of Cancer. 2003;103(2):153–160. [PubMed]
17. Toyooka S, Suzuki M, Tsuda T, et al. Dose effect of smoking on aberrant methylation in non-small cell lung cancers. International Journal of Cancer. 2004;110(3):462–464. [PubMed]
18. Hong YS, Roh MS, Kim NY, et al. Hypermethylation of p16 in Korean non-small cell lung cancer patients. Journal of Korean Medical Science. 2007;22:S32–S37. [PMC free article] [PubMed]
19. Liu Y, Lan Q, Siegfried JM, Luketich JD, Keohavong P. Aberrant promoter methylation of p16 and MGMT genes in lung tumors from smoking and never-smoking lung cancer patients. Neoplasia. 2006;8(1):46–51. [PMC free article] [PubMed]
20. Kondo K, Takahashi Y, Hirose Y, et al. The reduced expression and aberrant methylation of p16 in chromate workers with lung cancer. Lung Cancer. 2006;53(3):295–302. [PubMed]
21. Dammann R, Strunnikova M, Schagdarsurengin U, et al. CpG island methylation and expression of tumour-associated genes in lung carcinoma. European Journal of Cancer. 2005;41(8):1223–1236. [PubMed]
22. Furonaka O, Takeshima Y, Awaya H, Ishida H, Kohno N, Inai K. Aberrant methylation of p14, p15 and p16 genes and location of the primary site in pulmonary squamous cell carcinoma. Pathology International. 2004;54(8):549–555. [PubMed]
23. Tomizawa Y, Iijima H, Nomoto T, et al. Clinicopathological significance of aberrant methylation of RARβ2 at 3p24, RASSF1A at 3p21.3, and FHIT at 3p14.2 in patients with non-small cell lung cancer. Lung Cancer. 2004;46(3):305–312. [PubMed]
24. Suzuki M, Shigematsu H, Shames DS, et al. Methylation and gene silencing of the ras-related GTPase gene in lung and breast cancers. Annals of Surgical Oncology. 2007;14(4):1397–1404. [PubMed]
25. Kikuchi S, Yamada D, Fukami T, et al. Hypermethylation of the TSLC1/IGSF4 promoter is associated with tobacco smoking and a poor prognosis in primary nonsmall cell lung carcinoma. Cancer. 2006;106(8):1751–1758. [PubMed]
26. Divine KK, Pulling LC, Marron-Terada PG, et al. Multiplicity of abnormal promoter methylation in lung adenocarcinomas from smokers and never smokers. International Journal of Cancer. 2005;114(3):400–405. [PubMed]
27. Vaissière T, Hung RJ, Zaridze D, et al. Quantitative analysis of DNA methylation profiles in lung cancer identifies aberrant DNA methylation of specific genes and its association with gender and cancer risk factors. Cancer Research. 2009;69(1):243–252. [PMC free article] [PubMed]
28. Kersting M, Friedl C, Kraus A, Behn M, Pankow W, Schuermann M. Differential frequencies of p16(INK4a) promoter hypermethylation, p53 mutation, and K-ras mutation in exfoliative material mark the development of lung cancer in symptomatic chronic smokers. Journal of Clinical Oncology. 2000;18(18):3221–3229. [PubMed]
29. Belinsky SA, Nikula KJ, Palmisano WA, et al. Aberrant methylation of p16 is an early event in lung cancer and a potential biomarker for early diagnosis. Proceedings of the National Academy of Sciences of the United States of America. 1998;95(20):11891–11896. [PubMed]
30. Kerr KM, Galler JS, Hagen JA, Laird PW, Laird-Offringa IA. The role of DNA methylation in the development and progression of lung adenocarcinoma. Disease Markers. 2007;23(1-2):5–30. [PMC free article] [PubMed]
31. Zöchbauer-Müller S, Lam S, Toyooka S, et al. Aberrant methylation of multiple genes in the upper aerodigestive tract epithelium of heavy smokers. International Journal of Cancer. 2003;107(4):612–616. [PubMed]
32. Cirincione R, Lintas C, Conte D, et al. Methylation profile in tumor and sputum samples of lung cancer patients detected by spiral computed tomography: a nested case-control study. International Journal of Cancer. 2006;118(5):1248–1253. [PubMed]
33. Georgiou E, Valeri R, Tzimagiorgis G, et al. Aberrant p16 promoter methylation among Greek lung cancer patients and smokers: correlation with smoking. European Journal of Cancer Prevention. 2007;16(5):396–402. [PubMed]
34. Belinsky SA, Palmisano WA, Gilliland FD, et al. Aberrant promoter methylation in bronchial epithelium and sputum from current and former smokers. Cancer Research. 2002;62(8):2370–2377. [PubMed]
35. Feng Q, Hawes SE, Stern JE, et al. DNA methylation in tumor and matched normal tissues from non-small cell lung cancer patients. Cancer Epidemiology Biomarkers and Prevention. 2008;17(3):645–654. [PMC free article] [PubMed]
36. Lee JM, Bernstein A. p53 Mutations increase resistance to ionizing radiation. Proceedings of the National Academy of Sciences of the United States of America. 1993;90(12):5742–5746. [PubMed]
37. Byers TE, Vena JE, Rzepka TF. Predilection of lung cancer for the upper lobes: an epidemiologic inquiry. Journal of the National Cancer Institute. 1984;72(6):1271–1275. [PubMed]
38. Hanson CA, Miller JR. Non-traditional roles for the Adenomatous Polyposis Coli (APC) tumor suppressor protein. Gene. 2005;361(1-2):1–12. [PubMed]
39. Sherr CJ. D-type cyclins. Trends in Biochemical Sciences. 1995;20(5):187–190. [PubMed]
40. Jeanes A, Gottardi CJ, Yap AS. Cadherins and cancer: how does cadherin dysfunction promote tumor progression? Oncogene. 2008;27(55):6920–6929. [PMC free article] [PubMed]
41. Alvarez S, Germain P, Alvarez R, Rodríguez-Barrios F, Gronemeyer H, de Lera AR. Structure, function and modulation of retinoic acid receptor beta, a tumor suppressor. International Journal of Biochemistry and Cell Biology. 2007;39(7-8):1406–1415. [PubMed]
42. Weisenberger DJ, Campan M, Long TI, et al. Analysis of repetitive element DNA methylation by MethyLight. Nucleic Acids Research. 2005;33(21):6823–6836. [PMC free article] [PubMed]
43. Eads CA, Lord RV, Wickramasinghe K, et al. Epigenetic patterns in the progression of esophageal adenocarcinoma. Cancer Research. 2001;61(8):3410–3418. [PubMed]
44. Hawes SE, Stern JE, Feng Q, et al. DNA hypermethylation of tumors from non-small cell lung cancer (NSCLC) patients is associated with gender and histologic type. Lung Cancer. 2010;69(2):172–179. [PMC free article] [PubMed]
45. Virmani A, Rathi A, Heda S, et al. Aberrant methylation of the cyclin D2 promoter in primary small cell, nonsmall cell lung and breast cancers. International Journal of Cancer. 2003;107(3):341–345. [PubMed]
46. Kubo T, Yamamoto H, Ichimura K, et al. DNA methylation in small lung adenocarcinoma with bronchioloalveolar carcinoma components. Lung Cancer. 2009;65(3):328–332. [PubMed]
47. Castro M, Grau L, Puerta P, et al. Multiplexed methylation profiles of tumor suppressor genes and clinical outcome in lung cancer. Journal of Translational Medicine. 2010;8, article 86 [PMC free article] [PubMed]
48. Jeronimo C, Monteiro P, Henrique R, et al. Quantitative hypermethylation of a small panel of genes augments the diagnostic accuracy in fine-needle aspirate washings of breast lesions. Breast Cancer Research and Treatment. 2008;109(1):27–34. [PubMed]
49. Lewis CM, Cler LR, Bu DW, et al. Promoter hypermethylation in benign breast epithelium in relation to predicted breast cancer risk. Clinical Cancer Research. 2005;11(1):166–172. [PubMed]
50. Lee A, Kim Y, Han K, Chang SK, Hae MJ, Sang IS. Detection of tumor markers including carcinoembryonic antigen, APC, and cyclin D2 in fine-needle aspiration fluid of breast. Archives of Pathology and Laboratory Medicine. 2004;128(11):1251–1256. [PubMed]
51. Pu RT, Laitala LE, Alli PM, Fackler MJ, Sukumar S, Clark DP. Methylation profiling of benign and malignant breast lesions and its application to cytopathology. Modern Pathology. 2003;16(11):1095–1101. [PubMed]
52. Lehmann U, Länger F, Feist H, Glöckner S, Hasemeier B, Kreipe H. Quantitative assessment of promoter hypermethylation during breast cancer development. American Journal of Pathology. 2002;160(2):605–612. [PubMed]
53. Evron E, Umbricht CB, Korz D, et al. Loss of cyclin D2 expression in the majority of breast cancers is associated with promoter hypermethylation. Cancer Research. 2001;61(6):2782–2787. [PubMed]
54. Evron E, Dooley WC, Umbricht CB, et al. Detection of breast cancer cells in ductal lavage fluid by methylation-specific PCR. The Lancet. 2001;357(9265):1335–1336. [PubMed]
55. Sakuma M, Akahira JI, Ito K, et al. Promoter methylation status of the Cyclin D2 gene is associated with poor prognosis in human epithelial ovarian cancer. Cancer Science. 2007;98(3):380–386. [PubMed]
56. Tsutsui M, Iizuka N, Moribe T, et al. Methylated cyclin D2 gene circulating in the blood as a prognosis predictor of hepatocellular carcinoma. Clinica Chimica Acta. 2010;411(7-8):516–520. [PubMed]
57. Lossos IS, Czerwinski DK, Alizadeh AA, et al. Prediction of survival in diffuse large-B-cell lymphoma based on the expression of six genes. The New England Journal of Medicine. 2004;350(18):1828–1837. [PubMed]
58. Waki T, Tamura G, Sato M, Motoyama T. Age-related methylation of tumor suppressor and tumor-related genes: an analysis of autopsy samples. Oncogene. 2003;22(26):4128–4133. [PubMed]
59. Euhus DM, Bu D, Milchgrub S, et al. DNA methylation in benign breast epithelium in relation to age and breast cancer risk. Cancer Epidemiology Biomarkers and Prevention. 2008;17(5):1051–1059. [PubMed]
60. Brait M, Ford JG, Papaiahgari S, et al. Association between lifestyle factors and CpG island methylation in a cancer-free population. Cancer Epidemiology Biomarkers and Prevention. 2009;18(11):2984–2991. [PMC free article] [PubMed]
61. Belinsky SA, Liechty KC, Gentry FD, et al. Promoter hypermethylation of multiple genes in sputum precedes lung cancer incidence in a high-risk cohort. Cancer Research. 2006;66(6):3338–3344. [PubMed]
62. Zöchbauer-Müller S, Fong KM, Virmani AK, Geradts J, Gazdar AF, Minna JD. Aberrant promoter methylation of multiple genes in non-small cell lung cancers. Cancer Research. 2001;61(1):249–255. [PubMed]
63. Licchesi JDF, Westra WH, Hooker CM, Herman JG. Promoter hypermethylation of hallmark cancer genes in atypical adenomatous hyperplasia of the lung. Clinical Cancer Research. 2008;14(9):2570–2578. [PubMed]
64. Yue W, Dacic S, Sun Q, et al. Frequent inactivation of RAMP2, EFEMP1 and Dutt1 in lung cancer by promoter hypermethylation. Clinical Cancer Research. 2007;13(15):4336–4344. [PubMed]
65. Esteller M, Sanchez-Cespedes M, Resell R, Sidransky D, Baylin SB, Herman JG. Detection of aberrant promoter hypermethylation of tumor suppressor genes in serum DNA from non-small cell lung cancer patients. Cancer Research. 1999;59(1):67–70. [PubMed]
66. Yanagawa N, Tamura G, Oizumi H, Takahashi N, Shimazaki Y, Motoyama T. Promoter hypermethylation of tumor suppressor and tumor-related genes in non-small cell lung cancers. Cancer Science. 2003;94(7):589–592. [PubMed]

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