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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptNIH Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Opt Lett. Author manuscript; available in PMC May 3, 2011.
Published in final edited form as:
Opt Lett. Jan 15, 2011; 36(2): 139–141.
PMCID: PMC3086411
Fast voice-coil scanning optical-resolution photoacoustic microscopy
Lidai Wang, Konstantin Maslov, Junjie Yao, Bin Rao, and Lihong V. Wang*
Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, Campus Box 1097, One Brookings Drive, St. Louis, Missouri 63130-4899, USA
* Corresponding author: lhwang/at/
We developed a photoacoustic imaging system that has real-time imaging capability with optical resolution. The imaging system is capable of scanning at 20 Hz over a 9-mm range, and up to 40 Hz over a 1-mm scanning range. A focused laser beam provides a lateral resolution of 3.4 μm as measured in an optically nonscattering medium. Flows of micron-sized carbon particles or whole blood in a silicone tube and individual red blood cells (RBCs) in mouse ear capillaries were also imaged in real time, which demonstrated the capability to image highly dynamic processes in vivo at a micron-scale resolution.
Photoacoustic tomography (PAT), detecting ultrasound signals generated from photon absorption, provides optical absorption contrast in vivo for structural, functional, and molecular imaging [14]. PAT has two major modes: computed tomography (PACT) and focused-scanning tomography (PAM). In PAM, a single focused ultrasound transducer is mechanically scanned across a two-dimensional plane to acquire a three-dimensional image. At each scanned position, the acoustic time-of-flight provides depth information, and the acoustic focusing (AR-PAM) [4] or optical focusing (OR-PAM) [5] yields lateral resolution. Compared with PACT, PAM has a simpler implementation. Moreover, the spatial resolution of PAM can be scaled to image objects from sub-cellular organelles to organs [6]. However, most PAM systems utilize a precise ball-screw scanning mechanism, making it inherently difficult to achieve high scanning speed. This limitation calls for new technology to image dynamic processes in vivo with high spatial resolution. Fast scanning speed can sufficiently reduce the time to acquire a image so that dynamic processes can be imaged and motion artifacts can be mitigated. A high-speed voice-coil stage has been used to improve the scanning speed in an AR-PAM system, but only slow photoacoustic imaging was demonstrated [7]. Another PAM modality employed optical scanning with weak acoustic focusing to improve scanning speed [8]; however, the weak acoustic focusing limited signal-to-noise ratio (SNR).
We present a novel PAM system that can provide real-time cross-sectional (B-scan) imaging with optical lateral resolution. The system utilizes a voice-coil linear translation stage to achieve high-speed scanning. Tight optical focusing provides a high lateral resolution of 3.4 μm in optically nonscattering medium.
As shown in Fig. 1, a pulsed laser delivers light to the photoacoustic scanning probe through a single mode optical fiber. The laser beam is focused by a set of optical lenses and then reflected on an aluminum-coated prism. The delivered laser pulse energy may be varied from several tens of nJ to over 100 nJ. An ultrasound transducer (V2022 BC, Olympus NDT) is employed to detect photoacoustic signals. The ultrasound transducer focuses on the same spot as the optical beam through two prisms and an acoustic concave lens (6 mm aperture, 0.5 NA in water). The optical and acoustic components are attached to a base plate to form a scanning probe, which weighs less than 40 grams.
Fig. 1
Fig. 1
Schematic of voice-coil-driven fast-scanning OR-PAM.
The scanning probe is mounted on a voice-coil linear translation stage (VCS-1010, Equipment Solutions, Sunnyvale, CA, USA), to create a fast-scanning axis (x-axis). Photoacoustic signals are amplified by two amplifiers (ZFL-500LN+, Mini-circuits, NY, USA), and then acquired by a high-speed digitizer (DAQ) (ATS9350, Alazar tech Inc., Pointe-Claire, QC, Canada), using a sampling rate of 500 MHz. The imaging system is capable of scanning at 20 Hz over a 9 mm range, and up to 40 Hz over a 1-mm range. The B-scan frame rate of the imaging system is currently limited by the laser repetition rate of 4 kHz. In this paper, we demonstrate fast scanning ability over small scanning ranges. However, the scanning range can be readily increased by using a faster pulsed laser without sacrificing the scanning speed. The fast B-scan speed shortens the time to acquire a volumetric image, and the imaging system can work in a repetitive B-scan mode to image highly dynamic processes in real time.
As shown in Fig. 2(A), the lateral resolution of the PAM system was quantified by imaging a sharp edge in water. The full width at half maximum (FWHM) of the line spread function was 3.4 μm. The axial resolution was estimated to be 15 μm by the system bandwidth of 100 MHz and the speed of sound in biological tissue (~1.5 μm/ns). The penetration depth of the PAM was measured by imaging a needle inserted obliquely into a living-mouse leg. As shown in Fig. 2(B), the needle is visible at a depth of 1.2–1.4 mm into the tissue. Fig. 2(C) shows an image of the micro-vasculature in a nude mouse ear acquired in vivo at an optical wavelength of 570 nm. Fig. 2(D) is a close-up image of the dashed region in Fig. 2(C). Capillaries, the smallest blood vessel, are clearly resolved.
Fig. 2
Fig. 2
(A) Lateral resolution test on a sharp edge. ESF, edge spread function; LSF, line spread function. (B) Test of penetration depth by imaging a needle obliquely inserted into biological tissue. (C) In vivo maximum amplitude projection (MAP) image of mouse (more ...)
The SNR of the imaging system was measured by imaging a slice cut from a graphite rod (99.995%, Sigma-Aldrich Co., Saint Louis, MO, USA). The laser energy was 40 nJ per pulse, and the average SNR was 45 dB.
Flows of carbon particles with a mean diameter of 6 μm were imaged in real time in repetitive B-scan mode. As shown in Fig. 3(A), a syringe pump was used to control the flow speed. The PAM scanned in parallel with the tube at 20 Hz. Fig. 3(B) shows a representative B-scan image. The high-resolution real-time B-scan images provided the flow speed distribution along the depth direction of the tube. Fig. 3(C) shows the distribution along the x-axis of the photoacoustic signal at one depth as a function of time. The slope of each carbon particle trajectory is proportional to the flow speed at this depth. In the time domain, the flow speed v at the depth z can be written as equation M1, where k is a constant coefficient and vs is the scanning speed. When the scanning speed is much higher than the flow speed, which is the case here, the term equation M2 may be neglected. The flow speed may be approximated with equation M3. The flow speed can be more robustly calculated in the frequency domain. The two-dimensional Fourier transform of the data in Fig. 3(C) is shown in Fig. 3(D). Ft and Fx are the temporal and spatial frequencies associated with t and x, respectively. From the Fourier transformation, we have equation M4, where Nt and Nx are the sample lengths in the t and x axes, respectively. Hence, the flow speed v can be re-written as equation M5. Using the syringe pump, the coefficient k was calibrated to be 2.2. As shown in Fig. 3(E), a parabolic flow distribution along the depth direction was computed based on the real-time photoacoustic images.
Fig. 3
Fig. 3
(A) Schematic of the experiment setup for carbon particle flow measurement. (B) Representative B-scan flow image across the dashed-box area in Fig. 3(A). (C) Distribution of imaged carbon particles along the x-axis at one z-axis position versus time. (more ...)
Flows of whole bovine blood in a rubber tube were imaged at a 20 Hz B-scan rate. Variations in concentrations of RBCs were resolved. Fig. 4(A) shows a representative B-scan image. By changing the speed of the syringe pump, the flow speed within the penetration depth was varied from 0.04 to 0.25 mm/s. The same calibration k used for the carbon particle flow was employed to compute the blood flow speed. The measured flow speeds, as shown in Fig. 4(D), agree with the preset flow speed.
Fig. 4
Fig. 4
(A) B-scan image of whole blood flowing in a tube. (B) MAP along the z-axis of the B-scan image versus time. (C) Two-dimensional frequency spectrum of the data in Fig. 4(B). (D) Measured flow speed versus the preset values. NPA, normalized photoacoustic (more ...)
In a nude mouse ear, individual RBCs traveling in a capillary were imaged in vivo at a B-scan rate of 40 Hz. Fig. 5 shows individual RBCs flowing in the capillary, and a real-time video for individual RBCs flowing (Media 1) is attached. The flow speed was computed in the frequency domain, as shown in Fig. 5(C), using the coefficient k calibrated in the carbon particle flow experiment. Over five seconds, the average flow speed in the capillary was 0.13 mm/s, with a standard deviation of 0.03 mm/s.
Fig. 5
Fig. 5
(A) In vivo B-scan of single RBCs flowing in a mouse ear capillary. (B) MAP along the z-axis of the B-scan image versus time (Media 1). (C) Two-dimensional frequency spectrum of the data in Fig. 5(B). NPA: normalized photoacoustic amplitude; NSD: normalized (more ...)
All experimental animal procedures were carried out in conformance with the laboratory animal protocol approved by the Animal Studies Committee of Washington University in St. Louis.
To our knowledge, this is the first time PAM has demonstrated fast scanning capability at a frame rate of up to 40 Hz with optical resolution. Fast scanning OR-PAM can improve the imaging efficiency by tens of times, image highly dynamic processes in real time, and potentially reduce motion artifacts. This provides potentially broad applications. One example is functional imaging of RBC flows in a capillary in vivo. Although other imaging technologies, such as confocal microscopy, two-photon microscopy, and optical coherent tomography also can image RBC flows in a capillary in vivo, none of them can provide optical absorption contrast. Moreover, OR-PAM can measure oxygen saturation (SO2), which is an important parameter for functional imaging.
We acknowledge the help of Li Li in the system design. This work was sponsored in part by National Institutes of Health grants R01 EB000712, R01 EB008085, R01 CA134539, U54 CA136398, R01 EB010049, and 5P60 DK02057933. L. V. Wang has a financial interest in Microphotoacoustics, Inc. and Endra, Inc., which, however, did not support this work.
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