Rad51 filament formation is not inhibited in shu1Δ srs2Δ
cells. WT, shu1Δ
, and shu1Δ srs2Δ
cells were analyzed for the percentage of spontaneous CFP-Rad51 foci. Each experiment was done in triplicate with a total of 200 cells analyzed with SE plotted. Note that the strains also contain a WT Rad51–complementing plasmid because CFP-Rad51 is not fully functional. This configuration likely results in fewer Rad51 foci observed in srs2Δ
cells than we previously reported (Burgess et al.
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