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Rad51 filament formation is not inhibited in shu1Δ srs2Δ cells. WT, shu1Δ, srs2Δ, and shu1Δ srs2Δ cells were analyzed for the percentage of spontaneous CFP-Rad51 foci. Each experiment was done in triplicate with a total of 200 cells analyzed with SE plotted. Note that the strains also contain a WT Rad51–complementing plasmid because CFP-Rad51 is not fully functional. This configuration likely results in fewer Rad51 foci observed in srs2Δ cells than we previously reported (Burgess et al., 2009 blue right-pointing triangle).
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