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FIGURE 4:
Shu1 inhibits Srs2 recruitment to DNA breaks. (A) An I-SceI cut site was integrated into the rDNA adjacent to a tandem array of Tet repressor–binding sites (224xtetO). Location of the rDNA break is revealed by expression of a TetI fused to mRFP. Rad52-CFP and YFP-Srs2 were monitored for their recruitment to rDNA breaks in WT and shu1Δ cells expressing a GAL-I-SceI plasmid. The results are quantitated in the graph with SE plotted. (B) An I-SceI cut site was integrated at the URA3 locus on chromosome V adjacent to a tandem array of the Tet repressor–binding sites (336xtetO). Rad52-CFP and YFP-Srs2 were monitored in WT and shu1Δ cells for recruitment to the cut site in strains expressing a GAL-I-SceI plasmid. The results are quantitated in the graph with SE plotted.
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