Shu1 functions in the same pathway as Srs2 to suppress uaf30Δ rDNA recombination and alters Srs2 focus formation. (A) The frequency of rDNA recombination was measured in WT, shu1Δ, srs2Δ, shu1Δ srs2Δ, uaf30Δ, uaf30Δ srs2Δ, and uaf30Δ srs2Δ shu1Δ strains, and they were plotted with SD. Note the recombination frequency of the uaf30Δ shu1Δ strain was not conducted at the same time. (B) YFP-Srs2–expressing strains were analyzed for the percentage of spontaneous nuclear foci in WT, shu1Δ, uaf30Δ, and shu1Δ uaf30Δ cells. Images of Srs2 are shown with white arrowheads indicating foci. Each experiment was done in triplicate with a total of 400–500 cells analyzed. The graph shows the percentage of cells with foci along with the SE. (C) Cells expressing CFP-Rad51 were analyzed in WT and shu1Δ strains for the percentage of spontaneous nuclear foci. Each experiment was done in triplicate with a total of 150–200 cells analyzed with SE plotted. Note that the strains also contain a WT Rad51–complementing plasmid because CFP-Rad51 is not fully functional.
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