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Lateral connections in the olfactory bulb were previously thought to be organized for center–surround inhibition. However, recent anatomical and physiological studies showed sparse and distributed interactions of inhibitory granule cells (GCs) which tended to be organized in columnar clusters. Little is known about how these distributed clusters are interconnected. In this study, we use transsynaptic tracing viruses bearing green or red fluorescent proteins to further elucidate mitral- and tufted-to-GC connectivity. Separate sites in the glomerular layer were injected with each virus. Columns with labeling from both viruses after transsynaptic spread show sparse red or green GCs which tended to be segregated. However, there was a higher incidence of co-labeled cells than chance would predict. Similar segregation of labeling is observed from dual injections into olfactory cortex. Collectively, these results suggest that neighboring mitral and tufted cells receive inhibitory inputs from segregated subsets of GCs, enabling inhibition of a center by specific and discontinuous lateral elements.
One role of lateral inhibitory networks is mediating contrast enhancement of similar inputs in several brain regions (Kuffler, 1953; Sur, 1980; DeVries and Baylor, 1993; Ma and Suga, 2004). In sensory modalities with spatially organized input, as in vision, or input converted to space from a linear continuum such as frequency in audition, a spatially organized center–surround network is sufficient to enable contrast enhancement. In olfaction the stimulus is not spatial, but is transformed into a spatial pattern in the glomerular layer of the olfactory bulb (OB). The axons from sensory neuron that recognize structurally related odorants tend to be located in similar glomerular regions (Xu et al., 2003; Mori et al., 2006; Johnson and Leon, 2007). However, several lines of evidence show that there is not a definitive chemotopy in which glomerular location is a strict function of the molecular properties of the encoded odorant (Cleland and Sethupathy, 2006; Man et al., 2007; Soucy et al., 2009). Consequently, a spatially organized center– surround inhibitory environment would not achieve olfactory contrast enhancement because signal from nearest neighbors are not necessarily related; more distant interactions may be required.
The external plexiform layer includes the lateral dendrites of mitral and tufted (MT) projection neurons which form dendrodendritic synapses with inhibitory granule cells (GCs; Shepherd et al., 2004). Because the MT lateral dendrites can extend up to half of the OB circumference (Mori et al., 1983; Orona et al., 1984), each MT cell can form synapses with a large number of both proximal and distal GCs, providing a promising site for long range interactions. GCs outnumber MT cells by approximately 25 to 1 (Meisami and Safari, 1981; Frazier and Brunjes, 1988; Royet et al., 1998). In most brain regions the inhibitory to excitatory neuron ratio is closer to parity, or, as is the case in the cerebellum and thalamus, more excitatory than inhibitory neurons (Shepherd, 2004). The high ratio of inhibitory to projection neurons in the OB has not been explained, though given the very large disparity it seems reasonable to speculate that this property is central to odor processing.
We previously used transsynapticpseudorabies virus (PRV) tracing to demonstrate laterally distributed columnar organization of GCs (Willhite et al., 2006). The previous work did not address how two sites interconnect. When multiple glomeruli laterally influence another glomerulus, how is the network constructed to process the convergence? Here, we explore the convergence of the OB lateral inhibitory network using two PRV strains expressing GFP and RFP respectively (Billig et al., 2000; Smith et al., 2000; Banfield et al., 2003). Separate glomerular layer injections revealed sparse MT-to-GC connections within a column are generally segregated. Dual injections into separate sites in the anterior piriform cortex also resulted in segregated GC labeling within a column, suggesting that each MT cell forms synaptic connections with only a subset of the GCs within a column. We propose that these GC subsets allow summation of inhibition from multiple lateral sources, enabling combinatorial lateral inhibition consistent with the combinatorial nature of the olfactory code.
All of the animal use procedures in this study conformed to the Guide for the Care and Use of Laboratory Animals (1996, National Academy of Sciences) and were approved by the Yale Institutional Animal Care and Use Committee.
Attenuated Bartha-PRV strains which were further modified to express enhanced green fluorescent protein or mRFP1 under control of the cytomegalovirus immediate early promoter, PRV-152 and PRV-614 respectively, were used for transneuronal tracing of synaptic pathways in this study. The double-stranded DNA pseudorabies virus should not be confused with the single-stranded RNA rabies virus also used in tracing studies (Miyamichi et al., 2010).
Sprague–Dawley rats age 6–8 weeks were used in this study. Detailed previously published protocols were followed for viral injections (Card and Enquist, 1999). Rats were anaesthetized with 75mg/kg ketamine and 5mg/kg xylazine by intraperitoneal injection and additional drug was added as necessary. PRV injection solution was prepared by adjusting the titer to 1×108 PFU/mL with phosphate buffered saline (PBS) and adding red or green fluorescent microspheres to mark the injection site (Molecular Probes, Eugene, OR, USA). 100nL of PRV was injected using a Hamilton syringe (Fisher Scientific, Pittsburgh, PA, USA) with a 33 gauge needle into the indicated areas using a stereotaxic instrument (Stoelting, Wood Dale, IL, USA). The rate of injection was 20nL/min, controlled by a Nano-Injector system (Stoelting, Wood Dale, IL, USA). After 15 min to allow diffusion of the bolus, the syringe was removed slowly.
Three days post-injection (70–74h), the animals were deeply anaesthetized using urethane or ketamize/xylazine overdose, then perfused transcardially with PBS followed by a 4% paraformaldehyde (PFA) solution in PBS. The brain was extracted after fixation and post-fixed in PFA overnight or for 3h for histological preparation.
Hundred micron (50 micron for histology) free-floating sections were prepared on a Leica VT1200S vibrating microtome. Sections that had not been infected with PRV 152 or PRV 614 were stained with mouse anti-NeuN (IHCR1001-6, Millipore, Billerica, MA, USA) for stereology. PRV 152 infected sections were stained with Anti-Caspase 3 (active, 04-439), Anti-Nestin clone rat-401 (IHCR1006-6), Anti-NG-2 (AB5320MI) also from Millipore, anti-doublecortin (sc-8066, Santa Cruz Biotechnology, Santa Cruz, CA, USA), or GAD65/67 Monoclonal Antibody (9A6, Assay Designs Ann Arbor, MI, USA). Antigen recovery was performed on every tenth 50 micron section for NeuN staining only by placing each in 500μL 0.1M Na-citrate pH 6 and placed at 99°C for 10 min. Sections were resuspended in 200μL PBS for 10 min, then replaced with 200μL of 2% BSA+1% Triton in PBS (blocking buffer) for 30 min, then stained with primary antibody overnight at 4°C. After wash with blocking buffer, sections were stained with a 1:2500 dilution of the anti-mouse Alexa 594 (Invitrogen, #11005) in blocking buffer at room temperature for 2h, then washed in blocking buffer. Anti-Caspase 3 (Active), Millipore Temecula, CA, USA.
Tissue sections were wet mounted in Vecta-Shield mounting medium containing DAPI (Vector Laboratories, Burlingame, CA, USA). Sections were examined using a Leica DM5500 fluorescent microscope or an Olympus Fluoview 1000 confocal microscope. Images were manipulated for brightness and contrast, and cropped using Adobe Photoshop version 6.0.1. or Photoshop Elements version 2.0.2. Distance measures and volumes were not adjusted for shrinkage of samples due to tissue processing. Diagram figures were created using Microsoft PowerPoint 2003, and other graphs using MATLAB V.220.127.116.117, and further manipulated in Adobe Photoshop.
Maps of the fluorescent intensity in the granule cell layer (GrCL) were constructed with OdorMap Builder (Liu et al., 2004). While the software was designed to display odor activation patterns in the glomerular layer, it is capable of constructing an intensity plot of any given set of sections through any selected lamina. The region immediately deep to the internal plexiform layer was traced for each 100 micron OB section.
Total neuron number as assessed by NeuN positive cells in the GrCL was determined using the optical disector method (Sterio, 1984; Gundersen et al., 1988). Every 10th 50 micron coronal section was used for random sampling from five animals. The GrCL, defined by the boundaries of the internal plexiform layer superficially and the rostral migratory stream deep, was outlined using StereoInvestigator software and hardware interfaced with a Leica DM5500 fluorescence microscope equipped with an automated stage (MBF Bioscience, Williston, VT, USA). See Table Table11 for additional stereological methods.
Stochastic MT-to-GC connectivity models were generated in MATLAB V.18.104.22.1687 (R2009a TheMathWorks). The probabilities for red and green labeling (and hence connectivity) were calculated from the measured number of red and green neurons as well as the volume of each column assuming a uniform density from the stereological result within each column. Based on the observed percent of red and green neurons within an individual column, the predicted percent of stochastically connected yellow neurons was determined from a binomial distribution assuming independent probabilities of a neuron being either red or green. The mean yellow percent is then given by (% red × % green) and its variance by [% red × % green × (1− % red × % green)].
The probability mass function and cumulative distribution functions were fit according to a binomial distribution for the stochastic model and a log-logistic distribution for the data due to the heavier tails. Plots were normalized to make the integrals equal to one. The entirety of the data was shifted by one to two standard deviations from zero (positively) in order to obtain a non-negative abscissa for the fitting function.
Dual PRV injections into medial glomerular layer sites separated by 500–800 microns were performed to determine the convergence or segregation of lateral connectivity. The tracer was targeted to the glomerular layer of the OB, as assessed by 1-μm fluorescent spheres included with the injections. After 1 day, labeling is observed in MT cells proximal to the injection site (data not shown). Three days after injection, widespread columnar labeling was apparent in the GrCL (Figure (Figure1A).1A). One possible expectation would be red, green, and yellow columns representing the segregated and convergent cases, respectively. The observed labeling showed some columns with only red or green GCs with no co-labeling or interdigitation. Columns labeled with both tracers occupying the same space, however, demonstrated a further subset of segregation – red and green labeled GCs within the same column, with apparently few co-labeled (yellow) cells (Figures (Figures2A–C).2A–C). This arrangement suggests that lateral dendrites innervating the EPL of a given column form sparse, segregated connections with GCs within the column. No columns were observed that contained only co-labeled cells. Pyramidal cells in the piriform cortex from the same animal show a high degree of co-labeling (Figure (Figure2D)2D) indicating that cells readily accept co-infection by both viral tracers. We interpret the cortical labeling to arise via centrifugal projections onto second-order GCs. Figure Figure33 shows the lateral extent of the labeling from the injection sites for one animal. While there is more labeling proximal to the injection than distal, the distribution is selective and with gaps not observed in a classical center-surround organization. No two injection sites were closer than 500 microns to prevent co-infection of first-order neurons.
We define first-order labeling as the subset of neurons that uptake virus directly from the injection, while second- and third-order are mono- and di-synaptic connections respectively. The majority of the second-order labeling occurred in GCs. The other candidate inhibitory cell population, the periglomerular cells, are not widely labeled using the PRV method (Figure (Figure1;1; Willhite et al., 2006). Because the indicator of infection is fluorescence, and the active production of fluorescent protein prevents distinguishing first-order from higher-order infections, the first-order neurons must be inferred from the injection site location, and the cell types known to innervate that location.
The candidate neuron types that form synapses with cells in both the glomerular layer and GCs consist of only three cell types: mitral cells, tufted cells, and a recently identified subset of deep short axon cell (dSAC; Figure Figure4;4; Eyre et al., 2008). Examination of the labeling patterns 1 day after infection of the glomerular layer to reveal the first-order population showed only limited labeling of the dSACs (data not shown), which are not thought to have lateral processes spanning significant distances other than the axon innervating the glomerular layer. This neuron subset is therefore unlikely to account for the widespread GC columns observed at later time points.
The number of observed convergently connected GCs as assessed by co-labeling was apparently few. However, to quantify if the observed labeling was more or less convergent than expected, the labeled neurons were counted, and the results were compared to a model of MT-to-GC connections based on a stochastic assumption. In the viral tracing experiment it is not possible to know with precision how many first-order neurons were infected. However, for any labeled volume of the GrCL, if the fraction of the total neurons labeled by each fluorophoreis known, then the probability that a cell should be co-labeled is also known by a normal distribution function (see Materials and Methods). As a simplified example, if 10% of the neurons were labeled red and 10% green, then 1% would be expected to be co-labeled.
We therefore determined the neuron density in the rat GrCL. This was approximately 550,000 neurons/mm3 with a standard deviation of 82,000 using NeuNimmunohistological staining as a neuronal marker to quantify using standard stereological procedures and the Cavalieri method to determine GrCL volume in five Sprague–Dawley rats age 6–8 weeks (see Table Table11).
Pseudorabies virus bearing genes for red or green fluorescent proteins were injected into the medial glomerular layer. The injection sites were separated by at least 500 microns to ensure separate first-order infection. After 3 days, determined to be adequate time for lateral spread, OB sections were prepared as described previously (Willhite et al., 2006).
The total number of red, green, and co-labeled neurons was determined for at least 10 dual-labeled columns per animal, 109 columns total. Due to the sparseness of the labeling, all neurons in each column were counted (12,503 total); stereological estimates were not used. Column boundaries were defined independently using red or green cells within a 40 micron radius to the next cell as a standard for nearest neighbor analysis. Boundaries of convergent columns were similarly defined using 40 microns with interdigitation of red and green as a standard. Columns without co-labeling were not further analyzed because the expected convergence value would be zero in all such cases. The filter sets used showed no cross-talk between the red and green channels.
Figure Figure5A5A shows the prediction of the stochastic connectivity model at the densities relevant to the data. The abscissa and ordinate represent the percentage of red and green cells respectively, and the color scale shows the expected random convergence. Figure Figure5B5B shows the actual data with each point representing a column on a background showing the stochastic case. The most heavily labeled columns, while visually robust, represented less than 7% of the average total neurons in the column volume, again indicating a sparse representation (three data points are not included in the figures for better visualization of the remainder). Most striking, however, is that all but 6 of the 109 randomly assayed columns showed a higher number of convergent neurons than expected. Further, none of the six was outside of one standard deviation from the expected mean.
The experiment was repeated mixing the two viruses in the same syringe and injecting in a single site in the medial glomerular layer. 100% convergence was not observed, suggesting that first-order infection is a low probability event. As expected, however, there was significantly more convergence than in the separate injections (Figure (Figure6).6). This control clearly shows that the two variants are able co-infect GCs.
We then subdivided the columns from the separate injection data set into three bins by proximity to the injection site. It was expected that there may be more convergence in proximal columns than distal ones; however, no obvious trend was apparent (data not shown). This may be due to the relatively large distance between injection sites (500 microns) that are necessary to maintain separate first-order infection, or may reflect a genuine distance-independent lateral connectivity environment.
The columns were then subdivided into three bins on the basis of depth within the GrCL. The more superficial GCs are thought to represent more mature neurons that have perhaps more fully integrated into the network than newly arrived GCs (Lledo et al., 2008). Perhaps due to the random nature of the injection sites, however, there were no differences in the observed convergence between these groups (data not shown).
We define a glomerular unit as the set of neurons associated with a single glomerulus. A key question is how the MT cells within a given glomerular unit connect to the local GCs. The sparse and largely segregated nature of the lateral MT-to-GC connection does not address the connectivity within a given glomerular unit. The 20–50MT cells connected to a single glomerulus may share a common set of GCs, or connect to subsets. Two days following glomerular layer injection, the labeling in the GC layer is local beneath the infected MT cells; the spread of virus along the lateral dendrites will only occur after 3 days. Three days following cortical infection, therefore, the labeling observed in the GC layer is interpreted to arise from somatic or proximal synaptic contact with infected MT cells (Card and Enquist, 1999). Supporting this model, no GC columns were observed from cortical injections which did not have labeled MT cells directly superficial to the column, while many such GC-only columns arose after glomerular layer injection. This property enabled us to address the question of MT-to-GC connectivity within the glomerular unit.
Dual PRV injections were performed in the anterior piriform cortex. Injections were separated by 500 microns to minimize the chance of first-order infection with both species. RFP and GFP labeled columns are apparent in the GC layer 3 days following injection (Figure (Figure7).7). The borders between the two can be strikingly sharp. When columns are labeled in both red and green, segregation is again observed, suggesting that MT cells connected to the same glomerulus form connections with different populations of GCs. We cannot rule out, however, the possibility that the source MT cell for a given labeled GC is from an adjacent glomerulus.
Though the columns are visually robust, the sparseness of the labeling (generally no more than 3% of total neurons present) raises the possibility that PRV is targeting a specific sub-population of cells. As expected, the majority of cells labeled in the GC layer are positive for GAD (data not shown). The number of known GC subtypes that can possibly account for the observed labeling is limited.
To determine if PRV targets only immature, or only mature neurons, we identified immature neurons in PRV infected tissue using doublecortin (DCX) expression as a marker. Immuno-staining revealed that PRV can infect, but does not exclusively infect, DCX positive neurons (Figure (Figure88A).
Recently, a GC subtype expressing nestin was found to selectively form synapses on the soma, as opposed to the lateral dendrites, of MT cells. Immuno-staining with anti-nestin showed a population of cells which appeared to be more sparse than the PRV staining. We were unable to find convincing evidence of co-localization of PRV with nestin positive cells, but clearly PRV is not selectively targeting this population (Figure (Figure8B).8B). We cannot rule out a possible unidentified subset which is selectively targeted, but have not found an exclusively targeted subset in the limited known GC sub-populations.
To determine if the virus also targets non-neuronal cell types in the OB, we immuno-stained for expression of glial fibrillary acidic protein (GFAP) or NG2 chondroitin sulfate proteoglycan (NG2) expression. As in other systems, PRV is able to infect these cell types in the OB (Figures (Figures8C,D).8C,D). Previous studies indicate that infection of glial cell types is non-productive – the virus can enter the cell but does not pass to new cells through this route (Ekstrand et al., 2008).
A recent study showed that PRV infection of neurons in vitro rapidly alters the cell morphology and physiology (McCarthy et al., 2009). Previous studies have suggested that the in vivo time course for neuronal damage is longer than 6 days, which is well beyond the maximal 3-day infection period used in this study (Smith et al., 2000; Irnaten et al., 2001; Davis et al., 2003; Glatzer et al., 2003; Rothermel et al., 2009). To determine if PRV is causing cell death in the OB in a more rapid time course than in the previously studied systems, we infected the glomerular layer as previously indicated and stained for the active form of caspase-3, an indicator of early stage apoptosis. In normal OB tissue, one or two cells would be labeled per 100 micron section. Infection with PRV for 3 days did not produce an increase in the number of cells with the active form of caspase-3 (data not shown). We then performed the same experiment, but infected with 10-fold lower titer to allow the animal to survive a 5-day time course. The cells near the injection site therefore should have been infected for the full 5-day period. There again was no increase in active caspase-3 positive cells over control tissue at any location. While it is possible that the increase in co-labeled cells over the expected value can be attributed to the in vitro neuronal changes noted in the McCarthy study, the degree of segregated labeling in addition to the lack of increased apoptosis in the OB suggest that such changes are not playing a major role in the 3-day in vivo time course.
This study examines connectivity relationships of the projection neurons within the OB. Transsynaptic tracing showed sparse and segregated lateral connections of MT cells with the inhibitory GCs. Further analysis demonstrated that the network does not fit a random connection assumption, and that the deviation from random connectivity is independent of distance or depth in the lamina. The probability of co-infection presumably depends on the specific locations of the injection sites.
Lateral inhibition in other sensory systems is thought to play a role in enhancing the contrast of similar inputs (Kuffler, 1953; Sur, 1980; DeVries and Baylor, 1993; Ma and Suga, 2004; Priebe and Ferster, 2008). The lateral interactions in the OB have the anatomical potential to act over much larger distances than these interactions in other sensory modalities (Mori et al., 1983; Orona et al., 1984), and have been shown to be physiologically capable of doing so in brain slice experiments (Xiong and Chen, 2002). It has been proposed, however, that the lateral network mediates gain control rather than contrast enhancement in olfaction (Schoppa, 2009). If this is the case, it is difficult to see what value would be gained by additional inhibitory units (GCs), where only a few with more synapses should serve the same function. Further, it is not clear how the gain control model address the role of adult turnover of GC populations.
Evidence from olfactory cortex has recently shown that a fairly constant fraction of pyramidal cells respond to monomolecular odorants which have very different levels of presynaptic glomerular activation (Rennaker et al., 2007; Poo and Isaacson, 2009). Mixtures of odorants do not necessarily produce more pyramidal cell activation, but different ensembles are activated that are not predictable on the basis of the two separate activation patterns (Stettler and Axel, 2009). Taken together, these data suggest that a degree of both gain control and contrast enhancement are performed in the OB prior to cortical activation.
The data presented in this study shows that, in the general case, MT cells from any given glomerulus form connections with GCs that are segregated from other lateral inputs. Several lines of evidence indicate that olfactory information is coded in a combinatorial manner (Sharp et al., 1975; Malnic et al., 1999; Araneda et al., 2000; Wilson, 2001; Soucy et al., 2009). The sparse MT-to-GC connectivity shown here suggests a mechanism for coincident inhibition to influence MT firing rates based on the specific combinations of surrounding MT activation, consistent with the combinatorial model. We speculate that the segregated connections may allow summation of coincident inhibition and enables MT cells to combine to perform the equivalent of a NOT-AND Boolean operation on other MT cells (Shepherd and Brayton, 1987), where an all-to-all MT-to-GC network would only be capable of NOT-OR operations. Slice recordings showing the need for summed inputs to induce lateral inhibition also suggest NOT-AND gating (Arevian et al., 2008), however, further physiological evidence is necessary.
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
PRV strains were provided by L. W. Enquist, Princeton University as a service of the National Center for Experimental Neuroanatomy with Neurotropic Viruses: NCRR P40 RRO118604 and the National Science Foundation under agreement No. IBN-9876754. We thank A. Bordey and S. Z. Young for use of a confocal microscope and technical assistance. We thank C. A. Greer for helpful comments and use of reagents. This work was supported by NIH/NIDCD grants DC000086 and DC008874.