Differences in diet between adults and infants predict that infants would have higher perchlorate intake doses than adults (11
). Pharmacological doses of perchlorate can reduce thyroid hormone production by competing with iodide for uptake by NIS (5
). Therefore, perchlorate toxicity may be modulated by iodide. For this reason, in addition to measuring perchlorate, nitrate and thiocyanate we also quantified iodide in the infant urine samples. The World Health Organization (WHO) defines iodine sufficiency for populations of children less than 2 years old as a median urinary iodine concentration ≥100 μg/L (22
). In this study, we measured iodide (not total iodine), the predominantly occurring and biologically available form of iodine (15
). Thus the group of infants we studied was iodine-sufficient based on a median iodide concentration of 125 μg/L. Median urinary iodide levels in these infants agree well with published studies of European infants with population medians ranging from 92–162 μg/L (23
Our study enrolled infants who met relatively stringent criteria for inclusion into feeding categories of breast milk, cow milk-based formula, or soy-based formula, so that we could compare perchlorate exposure by feeding method. describes a more detailed distribution of urinary concentrations of perchlorate, nitrate, thiocyanate, iodide and creatinine categorized by feeding method than presented by Cao et al (7
) Additionally, we present the results in concentration units because urinary creatinine excretion rates differ between breastfed and formula-fed infants (26
), and thus differences in creatinine-adjusted toxicant levels between different feeding groups would be influenced both by exposure and creatinine levels. Consistent with previously reported results (26
), we found that urine creatinine excretion was associated with the type of feeding, with breast fed infants (mean 12.99 mg/dL) excreting significantly less (p=0.03) creatinine than formula fed infants (mean 18.50 mg/dL). The proportion of detection for perchlorate was highest in breast-fed infants (95%), followed by infants consuming cow milk-based formula (86%) and infants consuming soy formula (68%). Breast-fed infants had significantly higher urinary perchlorate levels than infants who consumed cow milk-based formula (p=0.004) and soy formula (p<0.001) consistent with the findings of others (12
). Within the formula-fed infants, consumption of cow milk-based formula led to higher urinary perchlorate levels than did consumption of soy-based infant formula (p<0.001). A similar pattern was observed for thiocyanate: breast-fed (p<0.001) and cow milk-based formula (p=0.006) groups had significantly higher urinary thiocyanate levels than did the soy formula-fed group. The opposite was true for urinary nitrate levels: infants consuming cow milk-based formula (p=0.017) or soy formula (p=0.005) had significantly higher levels of urinary nitrate than the breast-fed group. Future measurement of nitrate levels in breast milk, infant formula, and water used to prepare infant formula may offer an explanation for this observation
Adjusteda concentrations of perchlorate, nitrate, thiocyanate, iodide and creatinine in urine samples collected from infants consuming breast milk, cow milk based formula or soybased formula.
The urinary iodide levels observed among the different feeding groups are consistent with the published iodide and iodine levels in breast milk and infant formula (12
). Infants consuming cow milk-based formula had significantly higher urinary iodide levels (p=0.003) than infants consuming soy formula. Previous analysis of different brands of infant formula found that soy formulas (N=9) contained average iodide concentration of (88 μg/L), about 40% lower than average iodide concentrations in cow milk-based formula (154 μg/L, N=15). Thus the relatively lower levels of iodide in soy-based infant formula lead to relatively lower urine iodide levels in infants consuming soy formula compared with cow milk-based formula.
We evaluated the correlation between the different analytes in urine. Perchlorate and iodide levels were positively correlated (r=0.55) in urine samples, while levels of the other anions showed no significant relation (See online supporting information, Table S2
). The positive correlation of perchlorate and iodide was found in both breast-fed and formula-fed infants, likely resulting from positively correlated levels of these two anions in breast milk and infant formula. One explanation is that the same food crop processes that concentrate perchlorate (e.g. water evaporation in edible leaves) may also concentrate iodine. The positive correlation of perchlorate and iodide in infant urine samples provides some evidence that perchlorate was not out-competing iodide for transport during lactation for the maternal/infant pairs in this study. Alternatively, the much higher levels of iodide in the infants could have masked a subtle perchlorate effect. Further research is needed to better characterize the potential of perchlorate to inhibit iodide transport from lactating woman to breastfed infant (13
Because some infants had up to four visits during the study and thus had up to four urine samples analyzed, we used a mixed linear model that accounted for correlations among multiple measurements from the same subject. Candidates for covariates used in the model were age, BMI, feeding method, and sex. Low intra-individual correlation coefficients (ICCs) were observed for these infants for perchlorate, perchlorate dose, nitrate and creatinine (rho=0.07, 0.12, 0.02 and 0.28, respectively). Compared with these analytes, thiocyanate showed higher intraclass correlation (rho=0.40), possibly because of its longer physiological half life (~ 6 days) compared with the other analytes (~8 hrs). Iodide had the highest ICC (rho=0.95) of analytes measured, possibly indicative of consistent fortification of formula with iodine and/or consistent iodine intake and secretion by the lactating mothers of the infants. Some of the variability observed could be due to differences in the timing of urine collection related to feeding among the different visits, or in the amount of diaper contamination. Alternatively, the exposure may have changed between different sampling periods. Breast milk perchlorate levels can vary significantly over a single day (12
) likely because of varied and episodic perchlorate intake from diet that will vary even more widely as maternal diet changes across seasons. Additionally, while perchlorate levels in infant formula are likely to vary less than those in breast milk, infants may have consumed different brands and volumes at different sampling times. A recent evaluation of perchlorate levels in infant formula found significant variability among formula manufacturers (121%), types (64%), and among different lots of the same product from the same manufacturer (32%) (28
). Another potential source of variability is the hydration state of the infant, which can be accounted for by dividing urinary perchlorate by urinary creatinine. Infants older than six months also could have varied and episodic perchlorate exposure from intake of different “solid” foods containing differing amounts of perchlorate (14
). Finally, variability in dose estimates would have been reduced by collecting 24 hr urine instead of spot samples, but 24 hr urine collection is impractical for infants. Based on all these sources of variability, we conclude that the study would have been strengthened by collecting multiple urine samples for each time period, and collecting further information on the complete dietary habits of the infants and breast feeding women.
The National Academy of Sciences (NAS) recently evaluated the health implications of perchlorate ingestion and suggested a perchlorate reference dose (0.7 μg/kg/day) that the EPA subsequently adopted (30
). The reference dose estimates an oral intake that is likely to be without an appreciable risk of deleterious effects during a lifetime. The NAS specified iodine-deficient pregnant women, fetuses and neonates as the life stages most sensitive to potential adverse health effects from perchlorate exposure. To provide toxicological perspective on our data, we estimated perchlorate dose on the basis of measured spot urine perchlorate and creatinine (21
). The equations used to calculate intake dose were based on children consuming cow’s milk or modified cow’s milk (21
). Therefore these dose estimation equations are only valid for infants with a similar relation of age and creatinine excretion. Both our data and a previous report (26
) find that breast-fed infants excrete significantly less creatinine than formula fed infants of the same age. Thus creatinine excretion estimates for breastfed infants must be adjusted accordingly to account for lower creatinine excretion rates compared with formula-fed infants. Therefore we adjusted the estimated perchlorate dose for breast-fed infants by multiplying by 0.69 to account for reduced 24 hr creatinine excretion in breast-fed infants relative to formula fed infants. The 0.69 correction factor was calculated based on the ratio of the geometrical means of creatinine levels in urine samples collected from breastfed infants compared with formula-fed infants. Further research is needed to better characterize differences in creatinine excretion in infants and thus improve estimates of toxicant intake doses in this susceptible population.
Distribution of estimated perchlorate exposure doses in 205 urine samples from the infants we studied are presented in . A more detailed distribution of perchlorate doses is presented in the online supporting information, Table S3
. Estimation of perchlorate dose in these infants revealed a median of 0.160 μg/kg/day, well below the EPA reference dose of 0.7 μg/kg/day. The median estimated perchlorate dose for infants is 2.4 times higher than the median dose estimated for adults in the U.S. population [0.066 μg/kg/day] (8
). The higher perchlorate doses estimated for infants, compared to adults, is likely due to higher consumption of perchlorate-containing foods and liquids per kilogram body weight. On the basis of perchlorate levels in breast milk and infant formula, previous reports have estimated that some infants may have perchlorate doses that exceed the RfD (10
). Our dose estimates, which are based on measured body weight and urinary levels of perchlorate and creatinine, find the central tendency of dose estimates to be below the RfD while some of the highest doses exceed the RfD. Of the 205 spot urine dose estimates, 9% exceeded the reference dose of 0.7 μg/kg/day; 6% of infants providing multiple samples had multiple perchlorate dose estimates above the reference dose. The infants with perchlorate dose estimates > RfD on more than one occasion are of more concern, given that perchlorate inhibition of iodide uptake would need to be chronic to result in thyroid inhibition (4
). No estimated doses exceeded the no effect level (7 μg/kg/day) calculated by Greer et al (4
) based on a 14 day study of controlled perchlorate intake (7 – 500 μg/kg/day) by healthy adults. The reference dose was derived by dividing the Greer et al (4
) no effect level by an uncertainty factor of 10 so that the reference dose would “protect sensitive populations and life stages” (15
). However sensitive life stages such as infants were not included in the Greer et al study (4
). Therefore the actual perchlorate dose that might cause a health effect in infants is not known. With the current study we confirm the prediction that infants are likely to intake higher perchlorate doses compared with adults (10
). Furthermore, these exposures to perchlorate and thiocyanate are associated with increased TSH and T4 levels in infant urine (7
). Further research is needed to clarify the health impact of perchlorate exposure in infants.
Estimated perchlorate dose (μg/kg/day) for 205 urine samples collected from 92 infants at ages ranging from 1–377 days.*
The magnitude of perchlorate dose was associated with the feeding method, with breast-fed infants having 18 of the 20 highest perchlorate doses. Estimated perchlorate doses for breast-fed infants were significantly higher than the doses of infants consuming cow milk-based formula (p<0.001), and infants consuming cow milk-based formula had significantly higher estimated doses than did soy formula-fed infants (p<0.001). These estimates are consistent with most food intake model predictions (10
). The one exception was that our estimated perchlorate doses for breast-fed infants (mean 0.420 μg/kg/day), were significantly lower (p<0.0001) than perchlorate dose estimates (mean 1.1 μg/kg/day) based on pooled breast milk samples collected from 13 lactating women in a previously published study (13
). Small studies such as this may be biased due to diet, geography and other variables that can significantly impact perchlorate intake. Further research is needed to better quantify infant exposure to perchlorate and other NIS inhibitors.
We also examined the influence of age on estimated perchlorate dose. While only 27% of the 205 samples were collected from infants less than two months of age, 11 of the 20 highest estimated perchlorate doses were from urine collected from infants ≤ 2 months of age. These findings agree well with previously published predictions that infants would be exposed to higher perchlorate doses during their first 2 months of life (12
). Declining perchlorate dose following the first two months of infancy is likely due to the declining ratio of milk intake per unit body weight as infants age (34
). We also compared our data with perchlorate exposure dose estimates for ages 6–11 months from the U.S. Food and Drug Administration Total Diet Study. On the basis of analysis of perchlorate in infant food and consumption data, the Total Diet Study estimated that U. S. infants of ages 6–11 months had a mean dietary perchlorate dose of 0.26–0.29 μg/kg/day (14
). Our perchlorate dose estimates for this same age group (mean, 0.208 μg/kg/day) agreed well with the FDA estimated doses. The close agreement of our convenience population data with the nationally representative FDA dose estimates supports the ability to generalize our findings.
Our finding of higher perchlorate exposure in breast-fed infants should not discourage women from breast feeding. Although perchlorate and other environmental chemicals in human milk leads to infant exposure through breastfeeding, the benefits of breastfeeding likely outweigh potential risk associated with these chemicals. Breast milk contains significant amounts of iodine that will likely reduce the ability of NIS inhibitors (e.g. perchlorate and thiocyanate) to block adequate iodide uptake by the thyroid. Indeed, the breast fed infants we studied were iodine-sufficient (median urinary iodide 129.5 μg/L), and thus it was less likely that exposure to NIS inhibitors resulted in inadequate iodine uptake by the thyroid. In addition to improving health by nutritional benefits, breast feeding offers immunological, developmental, psychological and economic advantages (35
). For these reasons, the American Academy of Pediatrics recommends breast feeding (39
In summary, this study of a convenience sample of infants provides novel data relevant to infant exposure to perchlorate, nitrate and thiocyanate. Our urine-based perchlorate dose estimates are consistent with previously published estimates that were based on dietary intakes. The magnitude of perchlorate exposure dose varies by feeding method: breast-fed infants had higher perchlorate doses than infants who consumed cow milk-based formula, and soy formula-fed infants had the lowest estimated perchlorate doses. The adjusted average perchlorate dose estimated for all infants in our study (0.255 μg/kg/day) is below the reference dose, however 16% of all infants (and 31% of breast-fed infants) had at least one perchlorate exposure dose in excess of the reference dose. The health impact of these perchlorate exposures is unknown. Regression models of this dataset indicate that urinary levels of perchlorate and thiocyanate were associated with increased urinary TSH in infants with iodide levels < 100 μg/L (7
) which is consistent with previous findings in U.S. women (6
). However, modeling of urinary thyroxine levels found that urinary perchlorate was associated with increased urinary thyroxine (7
), instead of the decreased thyroxine that would be biologically coherent with inhibition of thyroxine synthesis by inhibiting iodide uptake. Additionally, the small study size limits our ability to differentiate between the effects of perchlorate and feeding method. Additional studies are needed to characterize the health impact of exposure of infants to perchlorate and related anions.