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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
The appearance of SMD is not affected by a defect in cohesion.
The temperature-sensitive mcd1-1 and apn1/2 mcd1-1 cells were grown overnight at permissive temperature (23°C) in YPDA media. The logarithmically growing cells were shifted to non-permissive temperature (37°C), nocodazole was added and cells were incubated another 3 hours to arrest them at G2/M. Cells were then treated with MMS (0.1%, 15 min) in PBS at semi-permissive temperature (30°C) followed by incubation in YPDA with nocodazole. Cells were collected at the indicated times and processed for PFGE analysis.