The appearance of SMD is much great for a larger (Chr II) than a smaller chromosome (Chr III).
Logarithmically growing apn1/2 and triple mutants apn1/2 mre11 or apn1/2 rad54 were arrested in G2/M with nocodazole, treated with MMS (0.1%, 15 min) in PBS and returned to the YPDA+nocodazole medium. Cells were collected at the indicated times and processed for PFGE analysis. Chromosomes were visualized by ethidium bromide staining (A) and by Southern blotting (B) with a LEU2 probe that identifies both ChrII and III. Resection-related PFGE-shift of linear Chr III and formation of Chr III dimers are indicated (B). The SMD was estimated in the ethidium bromide stained gel (A) by comparing the amount of DNA material in the SMD region to DNA in the small chromosomes that experienced little breakage, “SMD / Chr (I+VI).”
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