PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

Logo of plosgenPLoS GeneticsSubmit to PLoSGet E-mail AlertsContact UsPublic Library of Science (PLoS)View this Article
 
From:
Published online Apr 28, 2011. doi: 10.1371/journal.pgen.1002059
An external file that holds a picture, illustration, etc.
Object name is pgen.1002059.g006.jpg Object name is pgen.1002059.g006.jpg
Figure 6
The appearance of SMD is much great for a larger (Chr II) than a smaller chromosome (Chr III).
Logarithmically growing apn1/2 and triple mutants apn1/2 mre11 or apn1/2 rad54 were arrested in G2/M with nocodazole, treated with MMS (0.1%, 15 min) in PBS and returned to the YPDA+nocodazole medium. Cells were collected at the indicated times and processed for PFGE analysis. Chromosomes were visualized by ethidium bromide staining (A) and by Southern blotting (B) with a LEU2 probe that identifies both ChrII and III. Resection-related PFGE-shift of linear Chr III and formation of Chr III dimers are indicated (B). The SMD was estimated in the ethidium bromide stained gel (A) by comparing the amount of DNA material in the SMD region to DNA in the small chromosomes that experienced little breakage, “SMD / Chr (I+VI).”
Articles from PLoS Genetics are provided here courtesy of
Public Library of Science