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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
MMS–induced killing of WT and various apn1, 2 derived mutants.
Logarithmically growing cells were arrested in G2/M with nocodazole, treated with MMS in PBS (0.1%, 15 min, 30 min) and returned to the YPDA+nocodazole medium for post-MMS incubation for 8 hours. The cell survivals of MMS-treated cells, without (A) and with (B) post-MMS incubation were done by plating cells to YPDA plates. The percentage is the ratio of colonies arising after MMS treatment vs mock treatment (done in triplicate). WT (), apn1/2 (), apn1/2 mag1(), apn1/2 ntg1/2 (♦) and apn1/2 ntg1/2 ogg1 (•).