Search tips
Search criteria 


Logo of wtpaEurope PMCEurope PMC Funders GroupSubmit a Manuscript
Hum Genet. Author manuscript; available in PMC 2011 April 28.
Published in final edited form as:
PMCID: PMC3083835

Reduced TFAP2A function causes variable optic fissure closure and retinal defects and sensitizes eye development to mutations in other morphogenetic regulators


Mutations in the transcription factor encoding TFAP2A gene underlie branchio-oculo-facial syndrome (BOFS), a rare dominant disorder characterized by distinctive craniofacial, ocular, ectodermal and renal anomalies. To elucidate the range of ocular phenotypes caused by mutations in TFAP2A, we took three approaches. First, we screened a cohort of 37 highly selected individuals with severe ocular anomalies plus variable defects associated with BOFS for mutations or deletions in TFAP2A. We identified one individual with a de novo TFAP2A four amino acid deletion, a second individual with two non-synonymous variations in an alternative splice isoform TFAP2A2, and a sibling-pair with a paternally inherited whole gene deletion with variable phenotypic expression. Second, we determined that TFAP2A is expressed in the lens, neural retina, nasal process, and epithelial lining of the oral cavity and palatal shelves of human and mouse embryos—sites consistent with the phenotype observed in patients with BOFS. Third, we used zebrafish to examine how partial abrogation of the fish ortholog of TFAP2A affects the penetrance and expressivity of ocular phenotypes due to mutations in genes encoding bmp4 or tcf7l1a. In both cases, we observed synthetic, enhanced ocular phenotypes including coloboma and anophthalmia when tfap2a is knocked down in embryos with bmp4 or tcf7l1a mutations. These results reveal that mutations in TFAP2A are associated with a wide range of eye phenotypes and that hypomorphic tfap2a mutations can increase the risk of developmental defects arising from mutations at other loci.


The rare dominantly inherited branchio-oculo-facial syndrome (BOFS [MIM 113620]) manifests predominantly as anomalies affecting first and second pharyngeal arches, including ocular (anophthalmia, microphthalmia, coloboma, or cataract), cervical, or periauricular cutaneous defects, and distinctive craniofacial features (including cleft lip, often incomplete, cleft/high arched palate, and malformed ears). Other manifestations include ectodermal anomalies (hypodontia, dysplastic nails, and prematurely grey hair), conductive hearing loss, inner ear malformation, nasolacrimal duct obstruction, ectopic dermal thymus, scalp cysts, renal anomalies, short stature, upper lip pits, and mild developmental delay (Lin et al. 1995; Tekin et al. 2009).

Mutations or deletions in TFAP2A have recently been identified in seven individuals with BOFS syndrome (Milunsky et al. 2008; Tekin et al. 2009). An affected mother and son pair had a 3.2 Mb deletion at chromosome 6p24.3, encompassing the TFAP2A locus. Four additional cases had de novo missense mutations located within the highly conserved basic region of the TFAP2A DNA binding domain (Milunsky et al. 2008) and one further case a complex mutation within the DNA binding and dimerization domains (Tekin et al. 2009) (Fig. 1). Deletions at chromosome 6p24 have also been described in two patients with ocular findings: one with anterior chamber anomalies and a 6p24–p25 interstitial deletion, and the other with sclerocornea associated with a 6p22.3–p24 interstitial deletion (Davies et al. 1999; Moriarty and Kerr-Muir 1992). These cases raise the possibility that TFAP2A might be more broadly involved in eye development, and that disruption of TFAP2A is associated with phenotypes other than BOFS.

Fig. 1
Results of molecular analysis of TFAP2A showing gene and protein structure, MLPA and custom array CGH. a Schematic representation of TFAP2A mRNA showing exon breakdown and number of bases encoded by each exon; splice indicators above depict TFAP2A full-length ...

TFAP2A is a member of the AP2 family of transcription factors that direct craniofacial and ocular development (Ahituv et al. 2004; Schorle et al. 1996; Zhang et al. 1996). In mice and zebrafish, Tfap2a is expressed in tissues that contribute to the developing eye, including lens primordium and neural crest (Bosserhoff et al. 1997; Knight et al. 2003; Mitchell et al. 1991; O’Brien et al. 2004). Tfap2a mutants in mice and zebrafish share similar craniofacial defects, but while Tfap2a null mice exhibit ocular phenotypes ranging from anophthalmia to defects in the developing lens and optic cup (Makhani et al. 2007; Schorle et al. 1996; West-Mays et al. 1999), no obvious eye abnormalities have been described in zebrafish tfap2a mutants (Knight et al. 2003). However, a link between tfap2a and eye development in zebrafish is suggested because simultaneous inhibition of tfap2a and tfap2c gives rise to embryos with abnormal eye and lens morphology (Li and Cornell 2007).

TFAP2A is a retinoic acid (RA)-responsive gene that acts in concert with other signals to regulate eye morphogenesis. For instance, development of the ventral eye is regulated by interactions between Hh (Hedgehog), RA, and fibroblast growth factor (Fgf) signals (Lupo et al. 2005; Take-uchi et al. 2003). The Wnt and Bmp (bone morphogenetic protein) pathways are also involved in eye formation and regionalization. In fish, enhanced Wnt signalling in the neural plate leads to anophthalmia (Cavodeassi et al. 2005; Heisenberg et al. 2001; Houart et al. 2002) and in mice, loss of function of the Wnt co-receptor Lrp6 leads to microphthalmia and coloboma (Zhou et al. 2008). Overexpression of the Bmp antagonist Noggin induces microphthalmia, coloboma, and other ventral abnormalities in chick, suggesting that endogenous Bmps have significant effects on the development of the optic cup (Adler and Belecky-Adams 2002). Indeed, in Bmp4+/− mice, the optic cup forms, but mature eyes show anterior segment abnormalities and an increased incidence of microphthalmia (Chang et al. 2001). Furthermore, recently we reported two mutations in the BMP4 gene that compromise eye development: a frameshift mutation in a family with anophthalmia-microphthalmia, retinal dystrophy and myopia, and a missense mutation in an individual with anophthalmia-microphthalmia and brain anomalies (Bakrania et al. 2008). In addition, mutations in the BMP family protein, GDF6, in humans and fish cause variable ocular anomalies including coloboma, microphthalmia, and retinal mis-patterning (Asai-Coakwell et al. 2009; Gosse and Baier 2009).

In order to elucidate the role of TFAP2A in eye development, we determined whether TFAP2A mutations are associated with congenital eye defects in humans outside the confines of BOFS and also investigated the embryonic expression pattern of the human gene. In human and animal models, many congenital eye defects are variably expressed suggesting that genetic and environmental factors can significantly affect penetrance. To explore this idea, we established a zebrafish model for studying tfap2a function in eye development to determine the phenotypic consequences of disrupting tfap2a function in zebrafish heterozygous or homozygous for mutations affecting Wnt and Bmp signalling. Our findings indicate that TFAP2A is expressed in developing neural and craniofacial tissues in humans as in other vertebrates, plays a critical role in eye morphogenesis in both humans and fish, that TFAP2A mutations are associated with a wide spectrum of ocular phenotypes, and compromised tfap2a function sensitizes the developing eye to the effects of deleterious mutations in bmp4 and tcf7l1a.

Materials and methods

Thirty-seven cases were selected from a larger cohort of patients with developmental eye anomalies recruited as part of a national anophthalmia study based at Moorfields Eye Hospital, London and Birmingham Children’s Hospital, Birmingham, UK. Informed consent for genetic analyses was obtained from all families, in accordance with Ethics approval 04/Q0104/129 by the Cambridgeshire 1 Ethics Committee. Three individuals, including one affected sibling-pair, demonstrated at least three typical BOFS features. The other cases had anophthalmia or microphthalmia associated with a partial phenotype of BOFS including facial anomalies (including cleft lip/palate or other facial clefting); deafness; skin defects (cutis aplasia in neck region, or upper lip pits); or a first degree relative with cleft lip/palate (one individual). A further 92 randomly selected cases with developmental eye anomalies from the cohort were screened for variations in the alternatively spliced exon 5.

We screened for TFAP2A mutations using PCR to amplify all coding exons from genomic DNA using primers located in the flanking introns. TFAP2A has three alternative transcription start sites, designated exons 1a (NM_003220.2), 1b (NM_001032280.2), and 1c (NM_001042425.1). In addition, there is an alternative carboxyl terminus due to alternative splicing of exon 5, designated 5a (M61156) (Buettner et al. 1993). PCR amplicons were generated for exons 1a, 1b, 1c, and 2–7 and alternative exon 5a (primer sequences and PCR conditions available on request). PCR products were treated with ExoSapIT (USB) and bi-directionally sequenced using standard techniques. Mutations were confirmed on a PCR product generated from a second genomic DNA sample. DNA from 192 healthy adults (European collection of cell cultures [ECACC]) was screened as controls.

We used the dosage-sensitive Multiplex Ligation-Dependent Probe Amplification (MLPA) technique (Schouten et al. 2002) to look for deletions or duplications of the whole TFAP2A gene or exons within the gene. Probes were designed to interrogate TFAP2A exons 2, 4, 5, 6 and 7 (probe sequences available upon request). The size of exons 1 and 3 and the presence of known polymorphisms restricted probe design for those two exons. Probes were diluted to 1.33 fmol/μl and 1.5 μl of this was added to a commercially available MLPA probe mix (Holoprosencephaly-P187; MRC-Holland, Amsterdam) to provide control peaks. The standard MLPA protocol was used, except the initial DNA denaturation volume was reduced by 1.5 μl to make room for the extra probe mix. MLPA PCR products (1 μl) were separated on an ABI3100 Sequencer and analysed using Genotyper (v3.7) software (Applied Biosystems, Foster City, CA, USA). TFAP2A copy number was determined by exporting peak heights into an Excel spreadsheet, specifically designed to assess the ratio of each test peak relative to all other peaks for the given individual. Further, ratios of test:control peaks and control:control peaks for each sample were compared to two normal individuals included in each run (Bunyan et al. 2004). The dosage quotient for a normal individual with two copies of TFAP2A is expected to be 1.0. Abnormal dosage quotients are 0.5 for a deletion, 1.5 for a duplication.

In order to characterize the TFAP2A deletion detected by MLPA, array Comparative Genomic Hybridization (CGH) was performed on cases 3 and 4 and both parents (father is case 5) using a custom-designed high resolution microarray. The array was designed using the online tool eArray 5.3.5 (Agilent Technologies Inc.). A 4 9 44k format was selected to cover chromosome 6p24.3 (chr6: 10,413,482–10,599,386 NCBI Build 36). All high-density oligonucleotides available in the eArray CGH library in this region were selected (1,725 probes in total). A second probe group of oligonucleotides at 7 bp spacing was generated for the same target region using the genomic tiling function (23,231 probes in total). A group of control probes, for normalization purposes, was generated from the high-density oligonucleotide library covering all autosomes at 300 kb spacing (10,257 probes in total). The arrays were hybridized according to the manufacturer’s instructions with slight modification: 300 ng male reference DNA (NA10851) and patient DNA samples were differentially labelled in Cy3 and Cy5, respectively, as previously reported (Redon et al. 2006), but reaction volumes were halved and only half of the labelled DNA was hybridized to the microarray. Array data were extracted using Agilent Feature Extraction Array data were normalized using a robust quadratic spline algorithm. A correction method based on the GC content around each probe was applied to remove the presence of genomic waves (Marioni et al. 2007). The log2 intensity ratio for each oligonucleotide on the array was then plotted against its genomic position to generate a CGH profile using the freely available software R ( The colour of each point corresponds to the Agilent score of each oligonucleotide. Scores range from 0 to 1 and increasing score indicates increasing reliability; scores are grouped and coloured as listed, 0–0.2 red, 0.2–0.4 yellow, 0.4–0.6 green, 0.6–0.8 blue, 0.8–1.0 black. Only oligonucleotide probes with a score greater than 0.4 were used to identify breakpoint regions.

Zebrafish embryos were raised at 28.5°C and staged according to Kimmel et al. (1995). Phenylthiourea (PTU) was applied to embryos to prevent melanization when necessary. Cartilages were stained with Alcian Blue (Kimmel et al. 1998). Mutant lines were bmp4st72 (Stickney et al. 2007) and tcf7l1am881 (Kim et al. 2000). Genotyping of tcf7l1am881/m881 and bmp4st72/st72 was performed as previously described (Kim et al. 2000; Stickney et al. 2007). The following morpholino sequence (GeneTools, Inc.) was used: tfap2a-splice 5′-GAAATTGCTTACCTTTTTTGATTAC-3′ (O’Brien et al. 2004). Morpholinos were reconstituted in Danieux buffer (Nasevicius and Ekker 2000) then diluted to 0.125 or 0.5 mg/ml. Embryos were injected with 1 nl of diluted morpholino at the 1–4 cell stage into the yolk immediately below the blastomeres. The phenotypes reported here come from three or more independent experiments. We observed comparable pharyngeal phenotypes to those reported in previous experiments, thus validating the specificity of the morpholino (O’Brien et al. 2004; Fig. 4a; iii/iv). Standard control random 25-mer morpholinos (Gene Tools) fail to give any phenotypes in our experimental conditions. All experiments were performed in accordance with regulatory standards and where appropriate regulated by the Animals (Scientific Procedures) Act 1986.

Fig. 4
Zebrafish studies demonstrating eye and craniofacial defects in tfap2a morpholino-injected larvae and enhanced phenotypes in Wnt and Bmp morphants. a Abrogation of tfap2a function leads to eye and craniofacial defects. (i, ii) Wild-type and tfap2a morpholino-injected ...

For in situ hybridization studies, digoxigenin (DIG)-labelled TFAP2A (NM_003220.2 nts 916–1253) probes were prepared by amplification from pCMV-SPORT6 vector containing full-length TFAP2A using primers 5′-TAATACGACTCACTATAGGGCTTGTCACTTGCTCATTG-3′ and 5′-ATTTAGGTGACACTATAGAGCTCCACCTCGAAGTAC-3′. The primers included T7 and SP6 primer sites. PCR was carried out using: 100 mM dNTPs, 0.1 mM each primer, 5 μl of 10× PCR amplification buffer and 1 U HotStar Taq DNA polymerase (Qiagen). Thermocycling was performed as follows: step 1, 94°C for 15 min; step 2.1, 94°C for 30 s; step 2.2, 72°C for 1 min (35 cycles); step 3, 72°C for 10 min. The 376 bp gel-purified PCR amplicon was used as a template to generate RNA probe. Digoxigenin-UTP was incorporated into riboprobes during in vitro transcription using the DIG RNA labelling mix (Roche) according to the manufacturer’s instructions. Antisense and sense probes were generated using T7 and SP6 polymerase, respectively. In situ hybridization was carried out on 8 μm microtome sections on 12.5 dpc mouse embryos, 15–22 Carnegie stage (CS) human embryos and foetal stage 2 (F2) human foetuses as described by Wilkinson (1992). Expression patterns were visualized using the NBT/BCIP system (Roche) and sections were mounted in VectaMount (Vector Labs) and analysed using the Axioplan 2 imaging system (Zeiss).


Mutation analysis

We identified three mutations in the cohort of 37 selected patients. Case 1 had a heterozygous de novo TFAP2A four amino acid deletion c.697_708del12 (p.Glu233_Arg236del) in the basic domain and case 2 had a heterozygous paternally inherited c.956T>C (p.Phe319Ser) and also a maternally inherited c.961C>T (p.Arg321Cys) variation in an alternative splice isoform, TFAP2A2 (Buettner et al. 1993). The c.961C>T (p.Arg321Cys) was present in 2/189 control samples suggesting that this is likely to be a polymorphism, but the c.956T>C (p.Phe319Ser) was not present in any of the 189 control samples and falls within a conserved residue (Fig. 1). The alternative transcript has only been defined in humans so far; however, the genomic region containing the c.956T>C variant is highly conserved throughout mammalian species (Fig. 1). In order to further elucidate the role of the alternatively spliced exon 5 in eye development, we screened an additional 92 individuals with eye development anomalies, principally anophthalmia, microphthalmia or coloboma, but found no variants. Nucleotide and codon numbers for case 1 refer to the TFAP2A 1a isoform (NM_003220.2) and for case 2 the TFAP2A2 isoform (M61156). We also identified further presumed non-pathogenic sequence variants in our 37 cases, including a heterozygous c.420C>T (p.Leu140-Leu) in an individual with unilateral anophthalmia and bilateral cleft lip and palate; further variants were c.−20C>G in the exon 1a 5′UTR, two known SNPs in intron 1 (rs654351 and rs654340), a heterozygous c.533_554delG in intron 3, previously described in normal controls (Klootwijk et al. 2003; Stegmann et al. 2001), a heterozygous c.1025 + 21G>A, c.1025 + 61G>A and a known SNP (rs303048) in intron 6, and a known synonymous SNP in exon 7 (rs3734391). The exonic and intronic variants are not predicted to disrupt 5′- or 3′-splice sites, or predicted exonic splice enhancer sequences (Cartegni et al. 2003; Gao et al. 2008).

Deletion analysis

Using MLPA, we identified two individuals (cases 3 and 4), a sibling-pair with classical features of BOFS, with a heterozygous deletion of all tested exons (see Fig. 1c). This deletion was also present in the father (case 5). The MLPA results were confirmed using a high-resolution oligonucleotide custom array (Fig. 1d). The array design is comprehensive in coverage and has a probe on average every 7 bp across a 185 kb region on chromosome 6p24.3 encompassing the TFAP2A gene. The array refined the telomeric breakpoint to within ~550 bp (chr6:10,474,246–10,474,793 NCBI36) and the centromeric breakpoint within ~265 bp (chr6:10,541,899–10,542,164 NCBI36). Only two genes are included in the deletion, TFAP2A and C6orf218. C6orf218 is a predicted gene that has no identified protein domain (Letunic et al. 2009) or known function.

Case histories

The clinical features of all cases are summarized in Table 1.

Table 1
Summary of molecular and clinical findings

Case 1, born to unrelated Caucasian parents, had classical features of BOFS including a high arched palate, prominent philtrum, narrow ear canals, abnormal pinnae, scalp, and eyebrow nodules (Fig. 2; Table 1). His ocular features included a right cystic remnant (disorganized globe/cyst), a mildly microphthalmic left eye with a reduced corneal diameter (6–7 mm), iris coloboma, primary aphakia, and a large posterior chorioretinal coloboma. There was no family history of eye or other developmental anomalies.

Fig. 2
Photographs of external features of Cases 1, 3, 4, and 5. Case 1 showing a full face with frontal bossing, R anophthalmia (wearing prosthesis), prominent philtrum; b left iris coloboma c right periorbital subcutaneous lump (presumed dermoid); d right ...

Case 2 was born with congenital heart and eye anomalies to unrelated parents (mother Caucasian, father Afro-Caribbean). The maternal great uncle (uncle of the grandfather, and now deceased) and his twin brother, the great grandfather of case 2 were both blind in one eye (aetiology unknown), the maternal grandmother has an abnormal kidney and there is a strong family history of postaxial polydactyly on the paternal side (paternal grandmother, aunt, and her two children). Case 2 had a right microphthalmic eye with sclerocornea, primary aphakia, and localized tractional retinal detachment and an extremely microphthalmic left eye with sclerocornea. Her systemic features, which are not classical for BOFS are listed in Table 1.

Case 3, previously described (Fielding and Fryer 1992), had bilateral orbital cysts associated with microphthalmic remnants, pronounced features of BOFS, severe learning difficulties with an autistic spectrum disorder and seizures, treated with carbamazepine (Table 1; Fig. 2).

Case 4 (younger sister of case 3) has also been previously described (Fielding and Fryer 1992), had relatively mild systemic features (Table 1; Fig. 2). She had a right orbital cyst, partially excised, associated with a microphthalmic eye with a subluxed cataractous lens, shallow anterior chamber and no fundal view, and left mild microphthalmia with a shallow anterior chamber, persistent pupillary membrane, iris hypoplasia, mild cataract, and an extensive chorioretinal coloboma involving optic disc and macula and bilateral nystagmus.

Case 5 (father of 3 and 4) demonstrates some mild, classical features of BOFS, including premature ageing changes (Table 1; Fig. 2). His ocular features are also subtle: he has normal anterior segments, but the right optic disc is dysplastic with an unusual blood vessel pattern; the left disc showed mild dysplasia.

Expression of TFAP2A in human eye

TFAP2A expression was analysed in mouse 12.5 dpc and human embryos CS 15–22 and foetal stage F2. Expression in the mouse was seen in the nasal process, palate and within the CNS (Fig. 3a). Expression was found to be broadly comparable in the human with expression in the nasal process, epithelial lining of the oral cavity, palatal shelves, tooth buds, and within the CNS (data not shown). During human eye development, TFAP2A was first seen in the anterior epithelium of the lens at CS 15 (Fig. 3b). At CS 18, TFAP2A was expressed more strongly in the anterior epithelium of the lens and also in the ganglion layer of the neural retina (Fig. 3c). At CS 22, TFAP2A was expressed in the equatorial region of the lens epithelium, and secondary lens fibres and throughout the ganglion cell layer of the neural retina (Fig. 3d). TFAP2A expression was still visible, but weaker in the retina of F2 human eyes (data not shown).

Fig. 3
In situ hybridization of TFAP2A in mouse and human developing embryos showing expression of TFAP2A. a Sagittal section of 12.5 dpc mouse showing expression of Tfap2a in the nasal process, palate, cerebellum, thalamus, and spinal cord. b Coronal section ...

Knockdown of zebrafish tfap2a function leads to eye and craniofacial defects

The zebrafish ortholog of TFAP2A is expressed in embryonic tissues that contribute to the eye, including the surface ectoderm and neural crest, and is required for craniofacial development (Barrallo-Gimeno et al. 2004; Knight et al. 2004; Knight et al. 2003; O’Brien et al. 2004). In order to address the role of zebrafish tfap2a in eye development, we used a morpholino (MO) anti-sense approach to knockdown tfap2a function (O’Brien et al. 2004). We found a range of morphological anomalies in the eyes of tfap2a morphant embryos, while overall embryo morphology was comparable to sibling control embryos (Fig. 4a; i, ii). Eye phenotypes were frequently asymmetric (Fig. 4a; vi, vii) and included microphthalmia (53%, Fig. 4a; vi-viii), mild coloboma (68%, Fig. 4a; vii, viii) and severe coloboma whereby ventral retinal tissue, including retinal pigment epithelium (RPE) protruded from the back of the eye towards the midline of the brain (13%, Fig. 4a; vii, viii). These phenotypes are not mutually exclusive and individual eyes can have all these features (Fig. 4a; vii, viii). We presume that variability of phenotype is due to incomplete abrogation of tfap2a function in all expressing cells.

Supporting previous studies (Barrallo-Gimeno et al. 2004; Knight et al. 2003, 2004; O’Brien et al. 2004), pharyngeal cartilages are affected in tfap2a morphants with the ceratohyal reduced in size and oriented medially instead of rostrally (Fig. 4a; iii, iv). Less severe defects were observed in more posterior arches (data not shown). We noted that there was concordance of severity between asymmetric eye and jaw phenotypes (arrow in Fig. 4a iv points to ceratohyal and palatoquadrate defects with an ipsilateral small eye and coloboma phenotype). These results demonstrate that, as in human and mice, zebrafish tfap2a is required for both craniofacial and eye development.

Combined abrogation of tfap2a and either bmp4 or tcf7l1a leads to eye phenotypes of enhanced severity

Many human congenital conditions are likely to be a consequence of mutations in two or more genes affecting the same pathway or developmental process. We therefore assessed if reduced tfap2a function sensitizes zebrafish embryos to the reduction or loss of function of other genes implicated in eye development. We selected mutations in the Bmp and Wnt signalling cascades, since both pathways are implicated in eye morphogenesis and in specification of tfap2-expressing neural crest cells (Adler and Belecky-Adams 2002; Kim et al. 2000). To test for genetic interaction, we reduced tfap2a function in embryos heterozygous or homozygous for mutations in tcf7l1a, which encodes a transcriptional effector of Wnt signalling, and bmp4, which encodes a Bmp ligand. Both bmp4st72/st72 mutants and tcf7l1am881/m881 mutants lacking zygotic activity of Tcf3 (tcf7l1am881/m881) have no overt eye phenotype (Dorsky et al. 2003; Stickney et al. 2007). We injected a concentration of tfap2a morpholino that does not give rise to any phenotype in a wild-type background (Fig. 4b-i; c-i) and assessed the phenotypic consequences in the mutant backgrounds. In both heterozygous and homozygous conditions, the m881 mutation in tcf7l1a enhanced tfap2a partial loss of function phenotypes. In heterozygotes, reduction of tfap2a gave a consistent coloboma phenotype (Fig. 4b; ii, iv) and in homozygotes, embryos showed fully penetrant anophthalmia (Fig. 4b; iii, iv). Homozygosity of the st72 mutation in bmp4 enhanced the severity of eye phenotypes upon reduction of tfap2a (Fig. 4c; ii, iii). In 100% of these embryos, eyes showed coloboma phenotypes and/or mild microphthalmia. These eye phenotypes are also present in 40% of st72 heterozygotes while the other 60% of embryos showed no overt eye phenotypes (Fig. 4c; i). Increasing the concentration of the tfap2a morpholino in the st72 background gave rise to body patterning defects (data not shown). Taken together, these results indicate that both overactivation of Wnt signalling due to the tcf7l1am881 mutation and reduced Bmp signalling due to the bmp4st72 mutation sensitize embryos to reduced tfap2a function.


We describe four cases of classical BOFS: one associated with a four-amino acid deletion (case 1), and three associated with a small chromosome deletion including the TFAP2A gene (cases 3–5). The whole gene deletion cases exhibited considerable phenotypic variability, ranging from severe (case 3), moderately severe (case 4) to mild in the father (case 5). Indeed, the father’s phenotype was so mild as to be unrecognized in the original report (Fielding and Fryer 1992), but with hindsight included several features, notably calcified styloid processes, related to second arch development and, more recently, features of premature ageing (Table 1). A previously described mother-son pair with a deletion encompassing TFAP2A and a less severe phenotype led to the suggestion that deletion cases might be milder due to complete haploinsufficiency. This is not supported here since our proband had very severe ocular, skin, and developmental anomalies, including autism, although the severity may be related to adverse first trimester pregnancy or genetic background effects.

Case 2, in whom we identified variations in the alternatively spliced exon 5a of TFAP2A2 (M61156) (Buettner et al. 1993), had severe eye defects, but did not exhibit a classical BOFS phenotype. She had a paternally inherited heterozygous c.956T>C (p.Phe319Ser) mutation in a conserved base, not present in controls, which segregated with polydactyly on the paternal side and a likely polymorphism c.961C>T (p.Arg321Cys) on the maternal side, also present in the great grandfather (with blindness in one eye), but not the grandmother who had an abnormal kidney. The TFAP2A2 isoform lacks the TFAP2A dimerization domain necessary for DNA binding, but reduces TFAP2A transcriptional activation by inhibiting interaction of TFAP2A with DNA (Buettner et al. 1993). However, it is unclear whether the mutation(s) constitutively increase or decrease function.

The eye phenotypes in individuals with TFAP2A mutations or deletions are extremely variable, from extreme microphthalmic remnants to dysplastic optic discs, probably influenced by mutations in other regulatory eye genes and/or gestational factors. Intermediate phenotypes, such as cataractous lenses and coloboma or cyst formation, indicate an important role for TFAP2A in lens formation and optic fissure closure. This is supported by our in situ hybridization studies which demonstrate TFAP2A expression in the developing human embryonic lens and ganglion cell layer of the neural retina, consistent with mouse expression studies (Bassett et al. 2007; Mitchell et al. 1991), and evidence from mouse knockout models. Germline knockout of mouse Tfap2a causes defects in lens development with persistent adhesion of the lens to the overlying surface ectoderm (Peters’ anomaly) and retinal defects including RPE transdifferentiation to neural retina and lack of a ganglion cell layer (West-Mays et al. 1999). However, although mouse conditional knockouts with selective reduction of Tfap2a in lens placode derivatives display similar lens defects, there are no retinal anomalies, suggesting these occur only if retinal Tfap2a levels are additionally compromised (Pontoriero et al. 2008). This appears to be in slight contrast to humans with mutations in the FOXE3 lens transcription factor gene, which is expressed predominantly in the developing human embryonic lens (Ugur Iseri et al. 2009). These individuals display not only severe lens defects, including primary aphakia (similar to Case 2) and Peters’ anomaly, but also colobomas, suggesting an inductive role for the lens in optic fissure closure (Ugur Iseri et al. 2009).

Zebrafish tfap2a morphants also have retinal and optic nerve colobomas. Despite comparable ocular defects in humans and zebrafish tfap2a morphants, including microphthalmia and retina pigment epithelial abnormalities, the phenotypes are less severe in fish probably due to partial redundancy of function of different zebrafish tfap2 genes. Abrogation of both tfap2a and tfap2c (Li and Cornell 2007) gives rise to very severe ocular malformation in zebrafish (GG and SW, unpublished data), suggesting a strong conservation of function of tfap2 genes across vertebrates.

The importance of genetic background to TFAP2A mutant phenotypes is reinforced by our zebrafish data showing strong genetic interactions between tfap2a knockdown and bmp4 or tcf7l1a mutations. Neither bmp4 nor tcf7l1a heterozygotes or homozygotes alone display overt eye phenotypes, but show strong ocular abnormalities upon partial abrogation of tfap2a, implying that human hypomorphic TFAP2A mutations might contribute to eye developmental disorders when on a background of additional mutation(s) in other genes regulating eye formation. Parents carrying such mutations are consequently at higher risk of having offspring with severe eye abnormalities.

The combined abrogation of zebrafish tfap2a and tcfl1a causes anophthalmia, which is almost certainly an early phenotype affecting specification on field or formation of the optic vesicles. A similar phenotype is seen upon combined maternal and zygotic loss of tcfl1a function and other situations where canonical Wnt/ß-catenin signalling is enhanced in the neural plate eye-forming region (Cavodeassi et al. 2005; Dorsky et al. 2003; Heisenberg et al. 2001; Houart et al. 2002; Kim et al. 2000). Abrogation of tfap2a may enhance activation of the Wnt pathway when combined with reduced tcfl1a function. Both tfap2a and bmp4 are expressed during gastrulation in non-neural ectoderm and later, either in the forming eye or surrounding crest cells, providing a broad period during which the combined genetic lesions could have deleterious consequences.

The extraocular phenotypes observed in BOFS provide a further opportunity to consider the role of interacting pathways. We observed digit abnormalities, including partial syndactyly, in our BOFS cases suggesting a role for TFAP2A in digit development. Interestingly, reduced expression of the mouse Tfap2a in limb bud mesenchyme causes postaxial polydactyly (Feng et al. 2008). Furthermore, the c.956T>C (p.Phe319Ser) mutation in TFAP2A2 was associated with polydactyly in two relatives of Case 2. We have previously described individuals with BMP4 mutations with eye anomalies combined with poly- and syndactyly (Bakrania et al. 2008). Evidence for a direct interaction of tfap2a with Smad2/3 (part of the Bmp4 signalling pathway) has been shown by chromatin immunoprecipitation studies (Koinuma et al. 2009). Further possible sites for interaction may include the oral cavity, nasal prominence and palatal shelves where there is expression of TFAP2A in human embryos, consistent with studies in other animals (Knight et al. 2003; Mitchell et al. 1991; Moser et al. 1997; O’Brien et al. 2004), and corresponding anomalies in individuals with BOFS, presumably due to reduced TFAP2A activity in these areas. Recently, the involvement of BMP4 in cleft lip, both overt and microform types, not unlike that seen in BOFS, has been reported (Suzuki et al. 2009). These findings, together with our zebrafish evidence of interacting pathways, suggest this phenotypic overlap may reflect an underlying genetic interaction.

This study reveals that TFAP2A is critical to eye, brain, and craniofacial development in humans in the context of classical BOFS, and may also play a role in isolated eye anomalies. We propose that the mutations and deletions described here would cause a reduction of TFAP2A in the developing lens and retina and subsequent degeneration of these structures leading to the various ocular defects seen in these patients. Furthermore, we demonstrate oligogenic effects between the tfap2a pathway and both the bmp and wnt signalling pathways in the zebrafish that are likely to be important mechanisms in the non-Mendelian origin of human developmental eye anomalies.


We gratefully acknowledge the help of many colleagues especially Heather Stickney and Rodrigo Young for help with zebrafish experiments and discussions, Dr Angela Martin for research co-ordination, Shane Giles and Tara Hill (Agilent Technologies) for technical support and supplying oligonucleotide array probe scores, clinical staff and colleagues including Dr Alison Salt, Mr Yassir Abou-Rayyah, Professor Graham Holder, Ms Marie Restori, Mr James Innes, Ms Jo Allard for their support. We are particularly grateful to the patients and their families for their willing and enthusiastic participation in the study. This study was supported by a Telethon Fellowship (GG), MRC project grant and Wellcome Trust programme grant (SW), Wellcome Trust [grant number WT077008] (NPC, TF, and SG), a Senior Surgical Scientist Award from the Academy of Medical Sciences/Health Foundation (NR) and generous grants from VICTA (RO), Polak Trust and VICTA (AW). The MRC/Wellcome-funded Human Developmental Biology Resource provided human embryonic material.

Contributor Information

Gaia Gestri, Department of Cell and Developmental Biology, UCL, London WC1E 6BT, UK.

Robert J. Osborne, Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK.

Alexander W. Wyatt, Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK.

Dianne Gerrelli, Human Developmental Biology Resource, UCL Institute of Child Health, London WC1N 1EH, UK.

Susan Gribble, Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK.

Helen Stewart, Department of Clinical Genetics, Oxford Radcliffe Hospital, NHS Trust, Oxford OX3 7LJ, UK.

Alan Fryer, Merseyside and Cheshire Clinical Genetics Service, Alder Hey Children’s Hospital, Liverpool L12 2AP, UK.

David J. Bunyan, Wessex Regional Genetics Laboratory, Salisbury District Hospital, Salisbury SP2 8BJ, UK.

Katrina Prescott, Yorkshire Regional Genetics Service, Department of Clinical Genetics, Chapel Allerton Hospital, Leeds LS7 4SA, UK.

J. Richard O. Collin, Department of Adnexal Surgery, Moorfields Eye Hospital, London EC1V 2PD, UK.

Tomas Fitzgerald, Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK.

David Robinson, Wessex Regional Genetics Laboratory, Salisbury District Hospital, Salisbury SP2 8BJ, UK; Human Genetics Division, Southampton University School of Medicine, Southampton, UK.

Nigel P. Carter, Wellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK.

Stephen W. Wilson, Department of Cell and Developmental Biology, UCL, London WC1E 6BT, UK.

Nicola K. Ragge, Department of Physiology, Anatomy and Genetics, University of Oxford, South Parks Road, Oxford OX1 3QX, UK; Department of Adnexal Surgery, Moorfields Eye Hospital, London EC1V 2PD, UK.


  • Adler R, Belecky-Adams TL. The role of bone morphogenetic proteins in the differentiation of the ventral optic cup. Development. 2002;129(13):3161–3171. [PubMed]
  • Ahituv N, Erven A, Fuchs H, Guy K, Ashery-Padan R, Williams T, de Angelis MH, Avraham KB, Steel KP. An ENU-induced mutation in AP-2alpha leads to middle ear and ocular defects in Doarad mice. Mamm Genome. 2004;15(6):424–432. [PubMed]
  • Asai-Coakwell M, French CR, Ye M, Garcha K, Bigot K, Perera AG, Staehling-Hampton K, Mema SC, Chanda B, Mushegian A, Bamforth S, et al. Incomplete penetrance and phenotypic variability characterize Gdf6-attributable oculo-skeletal phenotypes. Hum Mol Genet. 2009;18(6):1110–1121. [PubMed]
  • Bakrania P, Efthymiou M, Klein JC, Salt A, Bunyan DJ, Wyatt A, Ponting CP, Martin A, Williams S, Lindley V, Gilmore J, et al. Mutations in BMP4 cause eye, brain, and digit developmental anomalies: overlap between the BMP4 and hedgehog signaling pathways. Am J Hum Genet. 2008;82(2):304–319. [PubMed]
  • Barrallo-Gimeno A, Holzschuh J, Driever W, Knapik EW. Neural crest survival and differentiation in zebrafish depends on mont blanc/tfap2a gene function. Development. 2004;131(7):1463–1477. [PubMed]
  • Bassett EA, Pontoriero GF, Feng W, Marquardt T, Fini ME, Williams T, West-Mays JA. Conditional deletion of activating protein 2alpha (AP-2alpha) in the developing retina demonstrates non-cell-autonomous roles for AP-2alpha in optic cup development. Mol Cell Biol. 2007;27(21):7497–7510. [PMC free article] [PubMed]
  • Bosserhoff AK, Kondo S, Moser M, Dietz UH, Copeland NG, Gilbert DJ, Jenkins NA, Buettner R, Sandell LJ. Mouse CD-RAP/MIA gene: structure, chromosomal localization, and expression in cartilage and chondrosarcoma. Dev Dyn. 1997;208(4):516–525. [PubMed]
  • Buettner R, Kannan P, Imhof A, Bauer R, Yim SO, Glockshuber R, Van Dyke MW, Tainsky MA. An alternatively spliced mRNA from the AP-2 gene encodes a negative regulator of transcriptional activation by AP-2. Mol Cell Biol. 1993;13(7):4174–4185. [PMC free article] [PubMed]
  • Bunyan DJ, Eccles DM, Sillibourne J, Wilkins E, Thomas NS, Shea-Simonds J, Duncan PJ, Curtis CE, Robinson DO, Harvey JF, Cross NC. Dosage analysis of cancer predisposition genes by multiplex ligation-dependent probe amplification. Br J Cancer. 2004;91(6):1155–1159. [PMC free article] [PubMed]
  • Cartegni L, Wang J, Zhu Z, Zhang MQ, Krainer AR. ESEfinder: A web resource to identify exonic splicing enhancers. Nucleic Acids Res. 2003;31(13):3568–3571. [PMC free article] [PubMed]
  • Cavodeassi F, Carreira-Barbosa F, Young RM, Concha ML, Allende ML, Houart C, Tada M, Wilson SW. Early stages of zebrafish eye formation require the coordinated activity of Wnt11, Fz5, and the Wnt/beta-catenin pathway. Neuron. 2005;47(1):43–56. [PMC free article] [PubMed]
  • Chang B, Smith RS, Peters M, Savinova OV, Hawes NL, Zabaleta A, Nusinowitz S, Martin JE, Davisson ML, Cepko CL, Hogan BL, et al. Haploinsufficient Bmp4 ocular phenotypes include anterior segment dysgenesis with elevated intraocular pressure. BMC Genet. 2001;2:18. [PMC free article] [PubMed]
  • Davies AF, Mirza G, Flinter F, Ragoussis J. An interstitial deletion of 6p24–p25 proximal to the FKHL7 locus and including AP-2alpha that affects anterior eye chamber development. J Med Genet. 1999;36(9):708–710. [PMC free article] [PubMed]
  • Dorsky RI, Itoh M, Moon RT, Chitnis A. Two tcf3 genes cooperate to pattern the zebrafish brain. Development. 2003;130(9):1937–1947. [PubMed]
  • Feng W, Huang J, Zhang J, Williams T. Identification and analysis of a conserved Tcfap2a intronic enhancer element required for expression in facial and limb bud mesenchyme. Mol Cell Biol. 2008;28(1):315–325. [PMC free article] [PubMed]
  • Fielding DW, Fryer AE. Recurrence of orbital cysts in the branchio-oculo-facial syndrome. J Med Genet. 1992;29(6):430–431. [PMC free article] [PubMed]
  • Gao K, Masuda A, Matsuura T, Ohno K. Human branch point consensus sequence is yUnAy. Nucleic Acids Res. 2008;36(7):2257–2267. [PMC free article] [PubMed]
  • Gosse NJ, Baier H. An essential role for Radar (Gdf6a) in inducing dorsal fate in the zebrafish retina. Proc Natl Acad Sci USA. 2009;106(7):2236–2241. [PubMed]
  • Heisenberg CP, Houart C, Take-Uchi M, Rauch GJ, Young N, Coutinho P, Masai I, Caneparo L, Concha ML, Geisler R, Dale TC, et al. A mutation in the Gsk3-binding domain of zebrafish Masterblind/Axin1 leads to a fate transformation of telencephalon and eyes to diencephalon. Genes Dev. 2001;15(11):1427–1434. [PMC free article] [PubMed]
  • Houart C, Caneparo L, Heisenberg C, Barth K, Take-Uchi M, Wilson S. Establishment of the telencephalon during gastrulation by local antagonism of Wnt signaling. Neuron. 2002;35(2):255–265. [PubMed]
  • Kim CH, Oda T, Itoh M, Jiang D, Artinger KB, Chandrasekharappa SC, Driever W, Chitnis AB. Repressor activity of Headless/Tcf3 is essential for vertebrate head formation. Nature. 2000;407(6806):913–916. [PubMed]
  • Kimmel CB, Ballard WW, Kimmel SR, Ullmann B, Schilling TF. Stages of embryonic development of the zebrafish. Dev Dyn. 1995;203:253–310. [PubMed]
  • Kimmel CB, Miller CT, Kruze G, Ullmann B, BreMiller RA, Larison KD, Snyder HC. The shaping of pharyngeal cartilages during early development of the zebrafish. Dev Biol. 1998;203(2):245–263. [PubMed]
  • Klootwijk R, Hol FA, Wu M, Willemen JJ, Groenen P, Hamel B, Straatman H, Steegers-Theunissen RP, Mariman EC, Franke B. Genetic variation analysis of MLP, TFAP2A, and CSK in patients with neural tube defects. J Med Genet. 2003;40(4):e43. [PMC free article] [PubMed]
  • Knight RD, Nair S, Nelson SS, Afshar A, Javidan Y, Geisler R, Rauch GJ, Schilling TF. Lockjaw encodes a zebrafish tfap2a required for early neural crest development. Development. 2003;130(23):5755–5768. [PubMed]
  • Knight RD, Javidan Y, Nelson S, Zhang T, Schilling T. Skeletal and pigment cell defects in the lockjaw mutant reveal multiple roles for zebrafish tfap2a in neural crest development. Dev Dyn. 2004;229(1):87–98. [PubMed]
  • Koinuma D, Tsutsumi S, Kamimura N, Taniguchi H, Miyazawa K, Sunamura M, Imamura T, Miyazono K, Aburatani H. Chromatin immunoprecipitation on microarray analysis of Smad2/3 binding sites reveals roles of ETS1 and TFAP2A in transforming growth factor beta signaling. Mol Cell Biol. 2009;29(1):172–186. [PMC free article] [PubMed]
  • Letunic I, Doerks T, Bork P. SMART 6: recent updates and new developments. Nucleic Acids Res. 2009;37:D229–D232. Database issue. [PMC free article] [PubMed]
  • Li W, Cornell RA. Redundant activities of Tfap2a and Tfap2c are required for neural crest induction and development of other non-neural ectoderm derivatives in zebrafish embryos. Dev Biol. 2007;304(1):338–354. [PMC free article] [PubMed]
  • Lin AE, Gorlin RJ, Lurie IW, Brunner HG, van der Burgt I, Naumchik IV, Rumyantseva NV, Stengel-Rutkowski S, Rosenbaum K, Meinecke P, et al. Further delineation of the branchio-oculo-facial syndrome. Am J Med Genet. 1995;56(1):42–59. [PubMed]
  • Lupo G, Liu Y, Qiu R, Chandraratna RA, Barsacchi G, He RQ, Harris WA. Dorsoventral patterning of the Xenopus eye: a collaboration of Retinoid, Hedgehog and FGF receptor signaling. Development. 2005;132(7):1737–1748. [PubMed]
  • Makhani LF, Williams T, West-Mays JA. Genetic analysis indicates that transcription factors AP-2alpha and Pax6 cooperate in the normal patterning and morphogenesis of the lens. Mol Vis. 2007;13:1215–1225. [PubMed]
  • Marioni JC, Thorne NP, Valsesia A, Fitzgerald T, Redon R, Fiegler H, Andrews TD, Stranger BE, Lynch AG, Dermitzakis ET, Carter NP, et al. Breaking the waves: improved detection of copy number variation from microarray-based comparative genomic hybridization. Genome Biol. 2007;8(10):R228. [PMC free article] [PubMed]
  • Milunsky JM, Maher TA, Zhao G, Roberts AE, Stalker HJ, Zori RT, Burch MN, Clemens M, Mulliken JB, Smith R, Lin AE. TFAP2A mutations result in branchio-oculo-facial syndrome. Am J Hum Genet. 2008;82(5):1171–1177. [PubMed]
  • Mitchell PJ, Timmons PM, Hebert JM, Rigby PW, Tjian R. Transcription factor AP-2 is expressed in neural crest cell lineages during mouse embryogenesis. Genes Dev. 1991;5(1):105–119. [PubMed]
  • Moriarty AP, Kerr-Muir MG. Sclerocornea and interstitial deletion of the short arm of chromosome 6-(46XY del[6] [p22 p24]) J Pediatr Ophthalmol Strabismus. 1992;29(3):177–179. [PubMed]
  • Moser M, Ruschoff J, Buettner R. Comparative analysis of AP-2 alpha and AP-2 beta gene expression during murine embryogenesis. Dev Dyn. 1997;208(1):115–124. [PubMed]
  • Nasevicius A, Ekker SC. Effective targeted gene ‘knockdown’ in zebrafish. Nat Genet. 2000;26(2):216–220. [PubMed]
  • O’Brien EK, d’Alencon C, Bonde G, Li W, Schoenebeck J, Allende ML, Gelb BD, Yelon D, Eisen JS, Cornell RA. Transcription factor Ap-2alpha is necessary for development of embryonic melanophores, autonomic neurons and pharyngeal skeleton in zebrafish. Dev Biol. 2004;265(1):246–261. [PubMed]
  • Pontoriero GF, Deschamps P, Ashery-Padan R, Wong R, Yang Y, Zavadil J, Cvekl A, Sullivan S, Williams T, West-Mays JA. Cell autonomous roles for AP-2alpha in lens vesicle separation and maintenance of the lens epithelial cell phenotype. Dev Dyn. 2008;237(3):602–617. [PMC free article] [PubMed]
  • Redon R, Ishikawa S, Fitch KR, Feuk L, Perry GH, Andrews TD, Fiegler H, Shapero MH, Carson AR, Chen W, Cho EK, et al. Global variation in copy number in the human genome. Nature. 2006;444(7118):444–454. [PMC free article] [PubMed]
  • Schorle H, Meier P, Buchert M, Jaenisch R, Mitchell PJ. Transcription factor AP-2 essential for cranial closure and craniofacial development. Nature. 1996;381(6579):235–238. [PubMed]
  • Schouten JP, McElgunn CJ, Waaijer R, Zwijnenburg D, Diepvens F, Pals G. Relative quantification of 40 nucleic acid sequences by multiplex ligation-dependent probe amplification. Nucleic Acids Res. 2002;30(12):e57. [PMC free article] [PubMed]
  • Stegmann K, Boecker J, Richter B, Capra V, Finnell RH, Ngo ET, Strehl E, Ermert A, Koch MC. A screen for mutations in human homologues of mice exencephaly genes Tfap2alpha and Msx2 in patients with neural tube defects. Teratology. 2001;63(5):167–175. [PubMed]
  • Stickney HL, Imai Y, Draper B, Moens C, Talbot WS. Zebrafish bmp4 functions during late gastrulation to specify ventroposterior cell fates. Dev Biol. 2007;310(1):71–84. [PMC free article] [PubMed]
  • Suzuki S, Marazita ML, Cooper ME, Miwa N, Hing A, Jugessur A, Natsume N, Shimozato K, Ohbayashi N, Suzuki Y, Niimi T, et al. Mutations in BMP4 are associated with subepithelial, microform, and overt cleft lip. Am J Hum Genet. 2009;84(3):406–411. [PubMed]
  • Take-uchi M, Clarke JD, Wilson SW. Hedgehog signalling maintains the optic stalk-retinal interface through the regulation of Vax gene activity. Development. 2003;130(5):955–968. [PubMed]
  • Tekin M, Sirmaci A, Yuksel-Konuk B, Fitoz S, Sennaroglu L. A complex TFAP2A allele is associated with branchio-oculofacial syndrome and inner ear malformation in a deaf child. Am J Med Genet A. 2009;149A(3):427–430. [PubMed]
  • Ugur Iseri SA, Osborne RJ, Farrall M, Wyatt AW, Mirza G, Herbert H, Martin A, Collin JRO, Lathrop M, Ragoussis I, Ragge NK. Seeing clearly: the dominant-recessive role of human FOXE3 in eye development. Hum Mutat. 2009 in press. [PubMed]
  • West-Mays JA, Zhang J, Nottoli T, Hagopian-Donaldson S, Libby D, Strissel KJ, Williams T. AP-2alpha transcription factor is required for early morphogenesis of the lens vesicle. Dev Biol. 1999;206(1):46–62. [PubMed]
  • Wilkinson DG. In situ hybridization: a practical approach. IRL Press; Oxford: 1992.
  • Zhang J, Hagopian-Donaldson S, Serbedzija G, Elsemore J, Plehn-Dujowich D, McMahon AP, Flavell RA, Williams T. Neural tube, skeletal and body wall defects in mice lacking transcription factor AP-2. Nature. 1996;381(6579):238–241. [PubMed]
  • Zhou CJ, Molotkov A, Song L, Li Y, Pleasure DE, Pleasure SJ, Wang YZ. Ocular coloboma and dorsoventral neuroretinal patterning defects in Lrp6 mutant eyes. Dev Dyn. 2008;237(12):3681–3689. [PMC free article] [PubMed]