Dephosphorylation of CALK during flagellar assembly
Cells possess exquisite and cell type-specific mechanisms for specifying the structure and length of cilia and flagella. For example, flagella of wild type strains of the biflagellate green alga, Chlamydomonas reinhardtii
, are 11.6 µm +/− 1.1 (derived from [4
]). In addition to specifying steady-state length, Chlamydomonas
cells also detect and respond to assembling flagella of ~half-length. Levels of flagellar protein transcripts fall abruptly when newly assembling flagella reach ~ 6 µm [7
]. Moreover, the axoneme is structurally polarized. Some inner dynein arms are localized either in the proximal or distal half of the flagella [9
]; polyglutamylated tubulin exhibits proximal-distal polarity [11
]; and, beak-like structures within microtubule doublets of the axoneme are confined to the proximal half [12
]. Although cell biologists can use optical methods to measure ciliary/flagellar length, cells must use molecules, and to date no molecular marker for flagellar or ciliary length has been reported.
Here, we have examined Chlamydomonas
aurora-like protein kinase (CALK), a member of the aurora protein kinase family, as a possible molecular marker of flagellar length. Aurora protein kinases are known to regulate cilia in vertebrate cells and in Chlamydomonas
]. In both systems, the aurora proteins are in the desphosphorylated state in cells with organelles at steady-state length. When flagellar disassembly is triggered, however, the aurora proteins in both undergo changes in their phosphorylation state, and when the levels of the auroras are experimentally reduced, disassembly is inhibited. In our previous studies, we showed that phosphorylated CALK was a marker for the absence of flagella. The phosphorylated state of CALK occurred independently of the path to the deflagellated condition. Thus, CALK was phosphorylated on cells that were flagella-less because of their culture conditions (growth at an air-agar interface), due to experimentally-induced flagellar detachment, to environmentally- or developmentally-triggered flagellar shortening, or to basal body mutations incompatible with flagellar assembly [13
]. Because CALK is phosphorylated in cells lacking flagella and non-phosphorylated in cells with flagella at steady-state length, we have examined CALK phosphorylation during the flagellar assembly that occurs upon deflagellation. Specifically, we tested whether CALK phosphorylation state might be a marker of flagellar length.
We used SDS-PAGE and immunoblot analysis with an anti-CALK antibody to determine the phosphorylation state of CALK. The non-phosphorylated form migrates at a lower position on SDS-PAGE than the fully phosphorylated form [13
]. In resting cells with flagella at steady-state length, CALK was non-phosphorylated ( (left lane). And, when cells were deflagellated by pH shock, immunoblotting showed that CALK, most of which was in the cell body, was phosphorylated (, middle panel). Only a small portion of total CALK was in the flagella (, right panel) [13
]. Cell fractionation showed that both the non-phosphorylated and the phosphorylated forms of CALK were released by freezing and thawing into the freely soluble, cytoplasmic fraction of cells ().
CALK is Localized to the Cell Body and Undergoes Dephosphorylation during Flagellar Assembly
To assess the phosphorylation states of CALK during flagellar biogenesis, cells were harvested at the indicated times after flagellar assembly was induced by deflagellation () for determination of flagellar lengths and immunoblotting with CALK antibody. As shown in , CALK was in the fully phosphorylated state in deflagellated cells. To our surprise, when the newly assembling flagella reached about half-length, desphosphorylated CALK appeared; and at 45 min and thereafter, as the flagella regenerated to full length () [15
], the fully phosphorylated form had become undetectable ().
CALK dephosphorylation requires flagellar assembly
Previously, we showed that Chlamydomonas
depolymerizing kinesin, CrKinesin13, which also became phosphorylated upon deflagellation, gradually became dephosphorylated as the flagella assembled, CrKinesin13 [16
]. To determine whether dephosphorylation of either protein was related to flagellar biogenesis, we prevented deflagellated cells from initiating flagellar assembly by adding the flagellar assembly inhibitor colchicine just before deflagellation [5
]. Control, non-deflagellated cells incubated with colchicine retained full length flagella for 2 hr and possessed only the lower, non-phosphorylated forms of CALK () and CrKinesin13 (data not shown). Furthermore, colchicine did not block pH-shock induced deflagellation (not shown), nor did it block the concomitant phosphorylation of CALK or CrKinesin13 (). As expected [15
], the cells deflagellated in colchicine failed to assemble flagella (data not shown). Furthermore, even though assembly was blocked, the CrKinesin13 in the colchicine-treated cells became desphosphorylated (, left panel) similarly to that in control cells without colchicine (, right panel). In contrast, however, the CALK in the cells that failed to initiate flagellar biogenesis remained phosphorylated for the duration of the experiment (, left panel), unlike the CALK in control cells, which underwent the typical dephosphorylation during assembly (, right panel). Thus, dephosphorylation of CrKinesin13 after flagellar loss was unrelated to flagellar assembly, whereas dephosphorylation of CALK was linked to some aspect of flagellar biogenesis.
Flagellar Assembly Is Essential to Trigger Dephosphorylation of CALK but not CrKinesin-13
To determine whether CALK dephosphorylation was linked simply to initiation of flagellar assembly, we allowed deflagellated cells to begin flagellar assembly, blocked further assembly by adding colchicine, and then assessed the phosphorylation state of CALK. As shown in , cells that were allowed to initiate flagellar assembly for 15 minutes before assembly was blocked by colchicine (left panel) failed to dephosphorylate CALK when examined 2 hr after deflagellation (right panel). In contrast, CALK became fully dephosphorylated in the control (, right panel) cells that assembled full-length flagella (left panel). Thus, intiation of flagellar assembly per se failed to trigger CALK dephosphorylation. Taken together, our results indicated that dephosphorylation of CALK was not related simply to time after deflagellation or to initiation of flagellar assembly. Rather, the results suggested that dephosphorylation was related to flagellar length.
The phosphorylation state of CALK is linked to flagellar length
To test for a direct relationship between the phosphorylation state of CALK and flagellar length, we studied CALK in several mutants with flagella of varying lengths, and in wild type cells experimentally treated to generate flagella of varying lengths. Previously, we showed that CALK was constitutively phosphorylated in several strains of aflagellate cells, including wild type cells growing on agar plates, and IFT mutant cells dhc1b, ift88
, and fla10
]. CALK was similarly constitutively phosphorylated in the flagella-less mutants bld1
(an IFT52 mutant) and bld2
(an epsilon tubulin mutant) [18
]; and in cells with short flagella, fla14
(a retrograde IFT motor mutant) [20
] and pf22
(a flagellar dynein arm mutant) [9
] (). Thus, independently of the circumstances that led to the diminished length or absence of flagella, cells with flagella of less than half-length contained only the phosphorylated form of CALK.
CALK Phosphorylation State is Responsive to Flagellar Length
To examine CALK in cells whose flagella were around half-length (5 – 7 µm), we took advantage of the observation that cells deflagellated in a protein synthesis inhibitor draw upon a pool of flagellar precursors during regeneration that is sufficient to generate ~half-length flagella [15
]. As shown in , within 30 min after deflagellation in the protein synthesis inhibitor cycloheximide, cells had assembled half-length flagella, and then growth had ceased. As expected, CALK was phosphorylated upon deflagellation in the presence of cycloheximide. Furthermore, at 30 min after deflagellation, CALK dephosphorylation had begun to occur, but then had ceased. At 120 min, the flagella remained at half-length and the phosphorylation state of CALK remained at an intermediate level (). These results suggested that half-length represented a threshold length during flagellar biogenesis to which cells responded by triggering initiation of dephosphorylation of CALK. Thus, in these circumstances, a posttranslational modification of CALK was a molecular marker of ~half-length flagella.
To test whether the phosphorylation state of CALK was a more general marker of ~half-length, we generated cells with flagella of varying lengths using colchicine [15
] and assessed CALK phosphorylation. For this experiment, colchicine was added at the times indicated after deflagellation to block further assembly, and at 2 hr we determined flagellar length (, left panel) and assessed the phosphorylation state of CALK (right panel). CALK phosphorylation state indeed was a remarkably accurate indicator of flagellar length. In cells with flagella less than half-length (5 – 7 µm), CALK was phosphorylated, and in cells with flagella greater than ~half-length, CALK was dephosphorylated.
The differing states of CALK phosphorylation in two short flagella mutants also indicate that half-length is a threshold to which CALK is finely tuned
Because we had found that half-length was a threshold for the change in phosphorylation state of CALK in cells with pharmacologically generated half-length flagella, we anticipated that we would find similar evidence for a half-length threshold in mutants whose flagella were about half-length. Among 3 reported short (half-length) flagella mutants, shf1
, only shf1
retained the half-length phenotype and were studied further. The CALK in shf1
cells, whose flagella were between 6 – 8 µm in average length [21
] was mostly non-phosphorylated (). On the other hand, the CALK in shf2
mutants, whose flagella were also around half-length, was mostly phosphorylated. Occasionally, intermediate states of CALK phosphorylation were observed in shf1
cells (data not shown). As expected, the CALK in shf1
cells was capable of becoming phosphorylated upon deflagellation (see below).
Genetically-Specified Half-Length Is A Flagellar Threshold For Triggering CALK Dephosphorylation, And Cells Detect Absolute, Not Relative Flagellar Length
Because it was possible that the shf2
mutation rendered CALK incapable of becoming dephosphoryated, we generated shf2
/wild type dikaryons and tested for CALK dephosphorylation. We mixed wild type minus
(6145C) gametes with shf2 plus
gametes and allowed them to fuse to form zygotes whose cytoplasm, therefore, represented an amalgam of the 2 parental cytoplasms. Confirming earlier results [21
], the short flagella began to elongate soon after fusion (, left panel). And, strikingly, all of the CALK in the zygotes became dephosphorylated (, right panel). Thus, shf2
CALK was fully capable of becoming dephosphorylated. Taken together with the results above, these results that CALK exhibits different, all-or-none phosphorylation states in 2 short flagella mutants whose flagella were ~ half-length, indicate that half-length is a very finely monitored threshold during flagellar assembly.
CALK phosphorylation state is responsive to absolute flagellar length
We next tested whether the phosphorylation state of CALK was responsive to the absolute or relative length of flagella by use of long (lf
) and short flagella mutants. Of the four lf
mutants available [22
], lf1, lf2
, and lf3
cells have flagella of varying lengths, are defective in flagellar regeneration, and thus were not suitable for our assay [3
]. In contrast, lf4–3
null mutants have flagella of relatively uniform length of around 20 µm, and are able to regenerate flagella after deflagellation [4
] (, upper panel). As expected, CALK was phosphophorylated during flagellar loss and underwent dephosphorylation during flagllar regeneration. Of particular importance, dephosphorylation of CALK became detectable around 30 min after flagellar detachment when the flagella were about 6 µm long (, lower panel). Although CALK never became fully dephosphorylated in this experiment, the result that this long flagellar mutant initiated dephosphorylation of CALK when its flagella became ~6 µm long (i. e., half-the length of a wild type flagellum) indicated that cells are responsive to absolute and not relative flagellar length. Experiments with shf1
cells led to a similar conclusion. CALK in shf1
cells became phosphorylated upon deflagellation; and, even though flagellar assembly took much longer than in the lf4–3
cells (, upper panel), only when the flagella of the shf1
cells reached about 6 µm in length, was CALK dephosphorylation initiated (, lower panel). Thus, independently of the time required to assemble flagella or of ultimate flagellar length, cells detect absolute not relative flagellar length.
Translating flagellar length into a posttranslational modification of a flagellar regulatory protein
Our results that the phosphorylation state of the protein kinase CALK is a marker of flagellar length demonstrate for the first time that cells possess a mechanism for translating flagellar length into a posttranslational modification. The simplest models to account for such a sensing mechanism must include molecular events that occur only when cells begin to extend their flagella beyond a half-length threshold. For example, a cell body regulator of CALK dephosphorylation could transit through flagella by IFT and become activated only when it interacts with flagellar components uniquely present in the distal half of the flagellum. Or, in a time-of-flight model, the regulator might be activated only if its transit time in flagella is increased as a consequence of increased flagellar length. In the cilium-based sonic hedgehog signaling pathway, pathway activation depends on transit of cytoplasmic components into cilia [24
In a variant of these models, CALK itself could be modified by cycling through flagella. In any of these models, CALK dephosphorylation would fail in shf2 cells if the SHF2 protein were essential for assembly of unique components of the distal portion of flagella, or if SHF2 were a sensor essential for detecting half-length flagella and triggering formation of unique distal components.
Chlamydomonas cells could exploit the changes in CALK phosphorylation state for at least 3 interconnected, length-related features of flagella: control of flagellar length, specification during assembly of transport of proteins unique to distal versus proximal portions of flagella, and regulation of flagellar protein transcripts during flagellar assembly.
Two of the most prominent models for length regulation (see review ([1
]) are the feedback model that posits that assembly or disassembly of flagella occurs upon deviations from the genetically set length [25
], and the balanced point model that posits that length is determined by a passive mechanism that depends on length-dependent assembly and length-independent disassembly of flagellar components [5
]. A fundamental difference between the two models is that the feedback model depends on a sensor or a mechanism that detects flagellar length, whereas the balance point model does not.
The discoveries that several signaling molecules are essential for flagellar length control including cAMP [26
], calcium [6
], and protein kinases [3
] are consistent with a feedback model [28
]. And, our results here that CALK phosphorylation state is responsive to flagellar length provide the first molecular evidence for a length-sensing system. Whether a half-length sensor could be used to specify full length flagella remains unknown. Future studies on cells whose flagella are of different lengths - - uni
-flagella mutants [29
] and “long-zero flagella” cells generated by mechanical shearing [15
] - - should provide important insights into CALK phosphorylation and length regulation.
Previously, we temporally linked CALK phosphorylation to a nearly 4-fold increase in flagellar trafficking of IFT subcomplexes and to regulation of IFT cargo loading during regulated flagellar disassembly [17
]. Thus, it is possible that CALK phosphorylation state during flagella regeneration is used by cells to regulate loading of distal versus proximal cargo onto IFT particles to create flagellar polarity.
It is also possible cells exploit the phosphorylation state of CALK to regulate transcription of flagellar genes. Immediately after detachment of flagella, when CALK is phosphorylated, transcription of flagellar genes is activated. And, when flagella are ~half-length, CALK is dephosphorylated and transcription of flagellar genes is terminated [8
]. Although evidence is lacking in Chlamydomonas
that transcription of flagellar genes regulates length, ciliary length in zebrafish Kupffer’s vesicles is regulated by FGF-dependent gene transcription [30
In summary, our results demonstrate for the first time in any system the existence of a mechanism to translate flagellar length into a protein posttranslational modification. Our work lays the basis for a deeper understanding of control of flagellar assembly.