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Epidemiological studies have highlighted associations between the regular use of nonsteroidal anti-inflammatory drugs (NSAIDs) and reduced glioma risks in humans. Most NSAIDs function as cyclooxygenase-2 (COX-2) inhibitors that prevent production of prostaglandin E2 (PGE2). Since PGE2 induces expansion of myeloid-derived suppressor cells (MDSCs), we hypothesized that COX-2 blockade would suppress gliomagenesis by inhibiting MDSC development and accumulation in the tumor microenvironment (TME). In mouse models of glioma, treatment with the COX-2 inhibitors acetylsalicylic acid (ASA) or celecoxib inhibited systemic PGE2 production and delayed glioma development. ASA treatment also reduced the MDSC-attracting chemokine CCL2 in the TME along with numbers of CD11b+Ly6GhiLy6Clo granulocytic MDSCs in both the bone marrow and TME. In support of this evidence that COX-2 blockade blocked systemic development of MDSCs and their CCL2-mediated accumulation in the TME, there were defects in these processes in glioma-bearing Cox2-deficient and Ccl2-deficient mice. Conversely, these mice or ASA-treated wild-type mice displayed enhanced expression of CXCL10 and infiltration of cytotoxic T lymphocytes (CTL) in the TME, consistent with a relief of MDSC-mediated immune suppression. Antibody-mediated depletion of MDSCs delayed glioma growth in association with an increase in CXCL10 and CTLs in the TME, underscoring a critical role for MDSCs in glioma development. Lastly, Cxcl10-deficient mice exhibited reduced CTL infiltration of tumors, establishing that CXCL10 limited this pathway of immune suppression. Taken together, our findings show that the COX-2 pathway promotes gliomagenesis by directly supporting systemic development of MDSC and their accumulation in the TME, where they limit CTL infiltration.
Gliomas are the most common primary malignant brain tumors and present with dismal patient prognosis. Despite the need for curative treatments, definitive information regarding their etiology is lacking and identification of factors that influence the risk and prognosis remain undefined. Recent reports in epidemiology have exposed yet unexplained roles of immunosurveillance in gliomagenesis and survival of glioma patients. For example, the regular use of nonsteroidal anti-inflammatory drugs (NSAIDs) appears to be associated with reduced occurrence of malignant gliomas in humans (1,2). NSAIDs mediate their biological effects at least partially by suppression of cyclooxygenase (COX) 2 and its product prostaglandin (PG) E2, which in turn induces differentiation of immunoregulatory cells, such as myeloid-derived suppressor cells (MDSCs) and regulatory T cells (Tregs), in tumor-bearing hosts (3–6).
MDSCs consist of two distinct populations, Ly6GhiLy6Clo granulocytic MDSCs (gMDSCs) and Ly6G−Ly6Chi monocytic MDSCs (mMDSCs), and expand under pathological conditions including tumors (7). They negatively regulate both adaptive and innate immune responses by producing arginase-1, inducible nitric oxide synthase (iNOS; also known as NOS2), and reactive oxygen species (ROS) (7). Thus, MDSCs facilitate tumor progression by dampening immunosurveillance and are considered to be an important target for tumor immunotherapy. It is critical to gain a better understanding of the complex biological interactions between the immune system (MDSCs in particular) and developing gliomas.
In this regard, the chemokine CCL2 has been shown to play an important role in migration of MDSCs towards the tumor microenvironment (TME) (8). However, it is unknown whether the COX-2 pathway affects chemokine expression profiles that are responsible for MDSC accumulation in the TME.
In the current study, we evaluated our hypothesis that COX-2 blockade by NSAIDs would suppress gliomagenesis by inhibiting development of MDSCs and their accumulation in the TME using the Sleeping Beauty (SB) transposon-mediated de novo murine glioma model (9,10). Our findings in this study implicate significance of the COX-2 pathway in gliomagenesis through chemokine-mediated immune cell infiltration in the TME. Our data also suggest that NSAID-based prophylactic treatment strategies may provide therapeutic benefit for individuals at heightened risk of glioma development, assuming predictive risk factors can be identified.
Wild type (WT) C57BL/6 mice were obtained from Taconic Farms. C57BL/6-background mice deficient for the following genes were obtained from The Jackson Laboratory: Cox-2, Ccl2, and Cxcl10. Animals were bred and handled in the Animal Facility at the University of Pittsburgh per an Institutional Animal Care and Use Committee-approved protocol. As the Cox-2-deficient breeders were heterozygous, PCR using tail DNA was employed to genotype the mice per the vender’s instruction.
The procedure has been described previously (9). In vivo-compatible DNA transfection reagent (In vivo-JetPEI™) was obtained from Polyplus Transfection. The following DNA plasmids were used for glioma induction: pT2/C-Luc//PGK-SB13 (0.2 μg), pT/CAGGS-NRASV12 (0.4 μg), and pT2/shP53 (0.4 μg).
The procedure to establish glioma cell lines from SB transposon-mediated de novo murine gliomas has been described previously (10). The cultured cells were treated with the following NSAIDs at the indicated concentrations: ASA (Sigma Aldorich), celecoxib (Biovision), or dimethylcelecoxib (kindly provided by Dr. Axel H. Schönthal, University of Southern California, CA). Cell proliferation assay using WST-1 (Roche) was performed on the indicated days per the manufacturer’s instruction. The indicated groups were used as a control to determine the relative cell viability.
The PGE2 ELISA kit was obtained from R&D Systems. Assays were conducted per the manufacturer’s instructions.
We treated mice with ASA (10 mg/kg/day) or celecoxib (30 mg/kg/day) based on previous studies (11–14) and the U.S. Food and Drug Administration guidance (15). To treat neonates and unweaned mice with ASA, we prepared water-based solutions of 2 mg/ml ASA, and mice received the following volume of these solutions by oral gavage: 20 μl for the first week, 50 μl for the second week, and 100 μl for the third week after birth. When the mice were weaned on days 21 to 23 after birth, they started to receive the treatment via the following method: drinking water that contained 0.05 mg/ml ASAor diet that contained 150 ppm celecoxib.
The procedure has been described previously (16). The following primers and probes were obtained from Applied Biosystems: Ccl2 (Mm00441242_m1), Cxcl10 (Mm99999072_m1), a n d Nos2 (Mm01290688_m1).
The procedure to isolate BILs has been described previously (17,18). Due to few numbers of BILs available from each individual, BILs were pooled from 3 mice in a given group for further evaluation. Fluorescent dye-conjugated antibodies for flow cytometry were obtained as follows: anti-CD4 (VH129.19), anti-CD8 (53–6.7), and anti-Ly6C (AL-21) from BD Biosciences; anti-CD11b (M1/70), anti-CD107a (1D4B), anti-FoxP3 (NRRF-30), and anti-Gr-1 (RB6-8C5) from eBioScience; anti-Ly6G (1A8) from BioLegend; anti-COX-2 (SP21) from AbCam. Flow data were obtained using a Coulter EPICS Cytometer (Beckman Coulter) and nalyzed using WinList software, version 6.0 (Verity Software House).
The procedure has been described previously (10). Anti-Gr-1 (RB6-8C5) monoclonal antibody (mAb) was obtained from eBioScience; control IgG was obtained from Sigma-Aldrich. Mice with developing gliomas received intraperitoneal (i.p.) injections of these Abs (0.25 mg/dose) on days 21, 23, 25, and 27 after plasmid DNA transfection.
Student’s t-test was performed to analyze differences between two groups; one-way analysis of variance with Holm’s post hoc test was performed for multiple groups. Log-rank test was performed to analyze survival of mice with developing gliomas. All data were analyzed using R Environment, version 2.12.1. P<0.05 was considered to be statistically significant.
To address the effects of NSAID treatment on gliomagenesis, we first induced de novo gliomas in mice by intracerebroventricular transfection of NRas and small hairpin RNA against P53 using the SB transposon system (9,10). By day 21 post-injection, the induced tumors demonstrated pathological characteristics of WHO grade 2 diffuse fibrillary astrocytoma seen in humans and progressed to WHO grade 3 anaplastic astrocytoma by day 60 (Supplementary Fig. S1). These findings suggest that these tumors initially develop as low-grade gliomas (LGGs) and progress to high-grade gliomas (HGGs), as often seen in the progression of human gliomas.
Subsequently, we evaluated COX-2 expressions in these mice (Supplementary Fig. S2). Glioma tissues, especially late-stage tumors (i.e. day 60) expressed COX-2 at high levels whereas adjacent normal brain tissues did not demonstrate detectable levels of COX-2 expression (Supplementary Fig. S2A, left). Moreover, CD11b+ cells in the spleen and bone marrow consistently express COX-2 in the presence or absence of intracranial gliomas (Supplementary Fig. S2A, center and right, and S2B). These data suggest that the de novo gliomas express COX-2 whereas the tumors do not appear to enhance the baseline expression levels of COX-2 in the spleen or bone marrow.
We then established primary glioma cell lines from these COX-2-positive tumors to evaluate the direct effects of COX-2 inhibitors on in vitro growth (Fig. 1A). ASA did not exhibit significant growth inhibitory effects on cultured glioma cells at up to 10 μM (= 1.8 mg/L culture medium; Fig. 1A, left). In contrast, celecoxib (center) and dimethylcelecoxib (right) inhibited glioma cell growth at similar concentrations, which is consistent with the reported direct cell-growth inhibitory effects of these agents (19). Concurrent with Supplementary Fig. S2, cultured glioma cells produced PGE2 at high levels (P<0.001; Fig 1B). Moreover, both ASA and celecoxib significantly inhibited PGE2 production from glioma cells at a concentration of 10 μM (P<0.001; Fig. 1B). These data suggest that ASA inhibits PGE2 production from the glioma cells without affecting glioma cell growth while celecoxib inhibits both glioma cell growth and their PGE2 production.
Next, we used ASA and celecoxib in vivo to treat mice with developing gliomas (Fig. 1C). With regard to the dose to administer, it has been reported that 20 to 50% of administered reagents remain active for 8 to 24 h in the blood circulation (20). Therefore, in vivo administration of ASA at 10 mg/kg/day in mice would exhibit a similar concentration in the body to that tested in vitro (1.8 mg/L). We also determined the dose of celecoxib to deliver in vivo (30 mg/kg/day in mice) that is equivalent to the clinical use (400 mg/day in human) (13,14). Daily 10 mg/kg ASA treatment initiated at the time of glioma induction significantly inhibited glioma development in WT mice with 3 of 8 treated mice surviving longer than 120 days (P=0.0411; Fig. 1C, left). In contrast, we observed no therapeutic effects when the treatment was initiated on day 21 (Fig. 1C, center), by which time solid gliomas were established (Supplementary Fig. S1B). However, 30 mg/kg celecoxib was effective in inhibition of glioma development even when the treatment was started on day 21 (P=0.0269; Fig. 1C, right), suggesting an involvement of its direct inhibitory effect on glioma cell growth (19).
We then addressed whether these treatments would reduce plasma PGE2 levels in the mice with developing gliomas (Fig. 1D). The ASA treatment significantly reduced plasma PGE2 levels in glioma-free mice (P=0.012; Fig. 1D, left). The mice with gliomas at days 50 to 60 exhibited higher plasma PGE2 levels than those in the glioma-free mice (P<0.001), which were reduced by the daily ASA treatment. The mice receiving the ASA treatment starting at day 0 demonstrated slightly reduced PGE2 levels compared with the ones receiving treatment at day 21 (P=0.0457). As the tumor size was not significantly different between the ASA- vs. PBS-treated groups based on bioluminescence measurement (data not shown) (10), the observed reduction of PGE2 levels is not likely due to merely smaller size of tumors in ASA-treated mice. Consistent with these data, the celecoxib treatment significantly reduced PGE2 levels compared with the control treatment (P<0.001; Fig 1D, right). Taken together, these findings indicate that COX-2 blockade by NSAIDs may exert protective (rather than therapeutic) effects on gliomagenesis possibly through systemic PGE2 inhibition. Based on the findings that pharmacological concentrations of celecoxib directly inhibit the proliferation of glioma cells (Fig. 1A), we thought that daily in vivo administration of ASA in mice with developing gliomas would reveal unique immune-mediated mechanisms operating in vivo without directly inhibiting the growth of glioma cells.
The results discussed in Fig. 1 led us to examine the immunological TME in response to the NSAID treatment. To this end, we extracted total RNA from brains, spleens, and bone marrows of the mice treated with ASA to evaluate the expression levels of Ccl2 and Cxcl10; these chemokines are known to attract MDSCs (8,21) and activated T-cells (17,18), respectively. Glioma tissues exhibited high Ccl2 expression levels, which were significantly suppressed when daily ASA treatment initiated on day 0 (P=0.0251; Fig. 2A, top left). In contrast, although Ccl2 mRNA levels increased in the spleens and bone marrows of glioma-bearing mice compared with glioma-free mice, ASA treatment did not inhibit the Ccl2 expressions in these organs (Fig. 2A, top center and right). Similar to the Ccl2 expression profile, the mice with developing gliomas also exhibited higher expression levels of Cxcl10 in the TME than those in the glioma-free mice (P<0.001; Fig. 2A, bottom left). The daily ASA treatment further enhanced the Cxcl10 expression levels in the TME (P=0.0261). In contrast, we observed no alteration of Cxcl10 expression levels in either the spleen or bone marrow in the presence or absence of the ASA treatment (bottom center and right).
Although the data described above suggest that the ASA treatment would affect MDSC accumulation in the TME, the COX-2 pathway is also known to promote systemic MDSC development in tumor-bearing mice (5). Therefore, we sought to evaluate the status of MDSCs in the organs tested above. The ASA-treated mice exhibited significant decreases in both frequencies and absolute numbers of CD11b+Ly6GhiLy6Clo gMDSCs in the gliomas (P=0.032) and bone marrows (P=0.0199) when compared with the PBS-treated mice (Fig. 2B). In contrast, we observed no significant differences in CD11b+Ly6GloLy6Chi mMDSCs in these organs.
Since MDSCs are known to exert their biological effects by producing arginase-1 and iNOS (also known as NOS2) (7), we extracted total RNA from the sorted gMDSCs and mMDSCs to evaluate mRNA levels of these effector molecules (Fig. 2C). The ASA treatment significantly inhibited Nos2 expressions in gMDSCs in all the organs tested (left). However, the treatment significantly inhibited the Nos2 expression levels only in the glioma-infiltrating mMDSCs but not those in the other organs (P=0.050; right). We observed no effects in Arg1 expression levels by the ASA treatment in any of the three sites (data not shown).
Consistent with the significant increase of Cxcl10 observed in the TME (Fig. 2A, bottom left), the ASA-treated mice exhibited an increase in glioma-infiltrating CD8+ T-cells compared with the PBS-treated mice (P=0.020; Fig. 2D). Moreover, the CD8+ T-cells in the ASA-treated mice demonstrated increased cell surface levels of a degranulation marker CD107a [mean fluorescence intensity (MFI) levels 9.8 vs 6.4] (22), suggesting improved effector functions of these cells compared with ones in PBS-treated mice.
The results discussed above led us to determine the specific roles of Cox-2 gene in gliomagenesis. In particular, we directed our focus on the TME because we observed the most significant changes of both chemokine profiles as well as immune cell infiltrations in the brain. To this end, we induced de novo gliomas in Cox-2+/+, Cox-2+/−, and Cox-2−/− mice. The Cox-2−/− mice exhibited a delay in glioma development compared with the Cox-2+/+ or Cox-2+/− mice (P<0.0478), with 5 of 6 Cox-2−/− mice surviving longer than 150 days (Fig. 3A). The Cox-2+/− mice also exhibited prolonged survival (P=0.0255), with 4 of 14 mice surviving longer than 150 days, compared with the Cox-2+/+ mice, none of which survived longer than 119 days. Consistent with these data, plasma PGE2 levels were significantly decreased in the Cox-2+/− mice compared with the Cox-2+/+ mice (P<0.001), and the levels were further decreased in Cox-2−/− mice (P<0.001; Fig. 3B). When we evaluated the effects of Cox-2 gene status on chemokine expressions in the TME, the Cox-2−/− mice exhibited lower levels of Ccl2 (P≤0.016) and higher levels of Cxcl10 (P≤0.0032) than the Cox-2+/+ or Cox-2+/− mice (Fig. 3C). Consistent with this, the Cox-2−/− mice exhibited a decrease in glioma-infiltrating Gr-1+ MDSCs (P=0.0016; Fig. 3D, top) with a concurrent increase in CD8+ T-cells (P<0.001; bottom). Taken together, these data demonstrate that the glioma-bearing Cox-2−/− mice exhibit a similar phenotype to the ASA-treated mice and suggest that the ASA treatment exerts its main biological effects through suppression of the COX-2-PGE2 pathway.
To determine whether altered Cox-2 gene expression levels in glioma cells influenced their proliferation, we established glioma cell lines from glioma tissues obtained from the Cox-2+/+ and Cox-2+/− mice. Although we were unable to establish Cox-2−/− glioma cells due to the lack of consistent tumor formation in these mice, Cox-2+/+ and Cox-2+/− glioma cells proliferated at similar rates in vitro (Supplementary Fig. S3).
These data led us to hypothesize that the ASA treatment would induce dynamic changes in chemokine expression profiles in the TME, thereby reducing MDSCs and increasing CTLs. As CCL2 is known to be a primary chemokine that attracts MDSCs (8), we sought to evaluate direct impacts of CCL2 by inducing gliomas in Ccl2−/− mice. The Ccl2−/− mice exhibited a significant delay in glioma development with 3 of 10 mice surviving longer than 120 days (P=0.0188; Fig. 4A). In addition, the Ccl2−/− mice exhibited an increase in Cxcl10 expression levels in the TME compared with WT mice (P=0.0347; Fig. 4B). When BILs were analyzed, the Ccl2−/− mice exhibited a decrease in Gr-1+ MDSCs (P=0.0031; Fig. 4C, top) and an increase in CD8+ T-cells (P=0.021; bottom) compared with WT mice. Taken together, these data suggest an important role of CCL2 in accumulation of not only MDSCs but also CD8+ T-cells in the TME.
To gain a better understanding of the relationship between MDSCs and CD8+ T-cell infiltration in the TME, we treated glioma-developing WT mice with anti-Gr-1 mAb to deplete Gr-1+ MDSCs. Consistent with our previous data in type-1 interferon receptor-deficient mice (10), WT mice with developing gliomas that had received anti-Gr-1 mAb for systemic MDSC depletion exhibited a prolonged survival with 4 of 9 mice surviving longer than 120 days (P=0.0063; Fig. 5A). Furthermore, the mAb-treated mice exhibited an increase in Cxcl10 expression levels in the TME (P=0.0082; Fig. 5B), which was consistent with the observation in the Ccl2−/− mice (Fig. 4B). The mAb-treated mice exhibited a decrease in glioma-infiltrating Gr-1+ MDSCs (P=0.014; Fig. 5C, top) as well as an increase in CD8+ T-cells (P=0.023; bottom). Our combined results from COX-2 blockade, Ccl2 deficiency, and MDSC depletion have uncovered a previously undescribed role of MDSCs in suppressing the expression of CXCL10, a critical chemokine for CD8+ T-cell recruitment into the TME.
To address the specific roles of CXCL10 in gliomagenesis, we induced de novo gliomas in Cxcl10−/− mice. Cxcl10−/− mice with developing gliomas exhibited significantly shorter survival than WT mice (P=0.0171; Fig. 6A). The Cxcl10−/− mice also exhibited a decrease in CD8+ T-cell infiltration (P=0.0079; Fig. 6B) and in their CD107a expression levels (MFI levels 9.5 vs 16.7), revealing a potential functional deficiency in addition to reduced numbers. Glioma-infiltrating CD8+ T-cells in both the Cxcl10−/− and WT mice exhibited similar expression levels of CXCR3 (a receptor for CXCL10; Fig. 6C), which is consistent with previous reports demonstrating that CXCL10 in the TME rather than CXCR3 plays a predominant role in tumor-infiltration of CTLs (17,18). Collectively, the current study indicates important roles of the COX-2 pathway in gliomagenesis through systemic development of MDSCs and their CCL2-mediated accumulation in the TME along with a reduction of CXCL10-mediated tumor-infiltration of CTLs.
This is the first report documenting the immunological significance of the COX-2 pathway in gliomagenesis in mice. In the current study, treatment with ASA or celecoxib inhibited systemic PGE2 production and delayed glioma development. This was especially true when the treatment was initiated shortly after the glioma-induction, suggesting significant involvement of the COX-2 pathway in the early stage of gliomagenesis. We also demonstrated a pivotal role of MDSCs and the MDSC-attracting chemokine CCL2 in the COX-2-mediated gliomagenesis.
The role of PGE2 in MDSC development has been reviewed (23). In particular, Ep2 (one of PGE2 receptors)-deficient mice bearing 4T1 mammary carcinoma demonstrated a decrease of MDSC development and delayed tumor growth compared with WT mice (5). In the current study, we demonstrated for the first time that COX-2 blockade by NSAIDs as well as genetic deletion of Cox-2 suppress glioma development in mice primarily by inhibiting MDSC accumulation in the TME (Figs. 1–3). We also observed a negative correlation between MDSCs and degranulating (CD107a+) CD8+ T-cells in the TME (Figs. 4C and and5C),5C), suggesting that MDSCs suppress the effector function of CD8+ T-cells in the TME and thereby facilitate glioma progression.
Although the ASA treatment inhibited systemic PGE2 levels regardless of the timing to be initiated, it did not provide any therapeutic benefit when it was initiated after the tumor had already been established (Fig. 1 and Supplementary Fig. S1). Similarly, while homozygous Cox-2−/− mice demonstrated remarkably prolonged survival compared with Cox-2+/+ mice (P<0.0478), heterozygous Cox-2+/− mice demonstrated only modest, albeit significant, prolongation of survival and reduction in plasma PGE2 levels compared with Cox-2+/+ mice (Fig. 3A and 3B). Furthermore, the heterozygous Cox-2+/− mice did not demonstrate significant differences in chemokine expressions and BIL profiles compared with Cox-2+/+ mice (Fig. 3C and 3D). These data, together with remarkable suppression of PGE2 levels by the ASA treatment (Fig. 1B), imply that a substantial threshold of PGE2 suppression has to be achieved to observe the clinical benefit of this strategy. These observations also suggest that ASA administration in humans might be only beneficial in prophylactic settings but not in patients with clinically diagnosed gliomas. Consistent with our interpretation, a recent study has demonstrated that long-term use of ASA reduces cancer risks in human (24).
With regard to the effects of ASA on each of the two distinct MDSC populations (Ly6GhiLy6Clo gMDSCs and Ly6G−Ly6Chi mMDSCs), the ASA treatment preferentially reduced gMDSCs than mMDSCs in the organs tested (Fig. 2B). Taken together with a previous study demonstrating that under inflammatory conditions, expansion of gMDSCs occurs at a greater degree than that of mMDSCs (25), gMDSCs may be more susceptible than mMDSCs to PGE2-mediated expansion and NSAID-mediated suppression. With regard to their functional differences, several studies have collectively indicated that gMDSCs preferentially express arginase-1 through the STAT3 signaling whereas mMDSCs express iNOS through the STAT1 signaling (7). Of interest, this proposed paradigm contrasts to our data demonstrating that gMDSCs also express iNOS that can be inhibited by ASA (Fig. 2C). Therefore, it is possible that our observations are unique to gliomas. Further studies are warranted to elucidate the mechanisms underlying the suppressive effects of each of MDSC populations in both human and mouse cancers located in a variety of organs.
CCL2 is known to be one of the primary chemokines attracting MDSCs towards TME (8), which is consistent with our observation that genetic depletion of Ccl2 induced a significant decrease in MDSC accumulation in the TME (Fig. 4). In detail, both gMDSCs and mMDSCs express the receptor CCR2 although its expression levels have been shown to be higher on mMDSCs than gMDSCs (26), suggesting that the CCL2-CCR2 chemokine-receptor axis can mediate the migration of both gMDSCs and mMDSCs.
Simultaneously, our data showed that CCL2 blockade (and resulting inhibition of MDSCs) led to increases of Cxcl10 expression and CD8+ T-cell infiltration in the TME (Figs. 4 and and5).5). This contrasts to a previous study demonstrating that CCL2 mediates accumulation of activated T-cells in a glioma xenograft (27). We think that this difference reflects the immunocompetent status of mice in our spontaneous tumor model versus the xenograft model that requires immune deficient hosts. Given that CCR2, a cognate receptor for CCL2, is expressed in a wide range of immune cells including monocytes, MDSCs, Tregs, and effector T-cells (28), the CCL2-CCR2 axis operates in all cell populations that express the ligand CCL2 and/or the receptor CCR2 in our syngeneic glioma model. However, CCL2 in the TME is likely to recruit primarily MDSCs (8). Then, MDSCs inhibit the type-1 functions of DCs (29,30), including DC production of CXCL10 (18,31). Taken together, we propose that CCL2-attracted MDSCs inhibit CXCL10 production in the TME through suppression of DCs.
COX-2 and PGE2 promote de novo induction of Tregs (32). Consistent with this, the ASA treatment inhibited Treg accumulation in the TME in our model (Supplementary Fig. S4). In this regard, we have previously attempted to address a role of Tregs in gliomagenesis by mAb-mediated depletion (10) and observed no significant effects of Treg-depletion on glioma development. Therefore, we rather focused on the roles of MDSCs in the current study. To elucidate a role of Tregs further, we are currently incorporating a Treg-attracting chemokine CCL22 in the SB-mediated glioma induction system; this system will allow us to address specific effects of CCL22 in the TME and glioma-infiltrating Tregs attracted by CCL22. Furthermore, it has been shown that glioma cell-derived CCL2 promotes Treg migration (33). We will elucidate the impact of these chemokines on Treg development and accumulation in future studies.
Collectively, the findings in the current study reveal the important roles of the COX-2 pathway in gliomagenesis through an increase in MDSC development and accumulation as well as a decrease in CXCL10-mediated CD8+ T-cell infiltration in the TME. The data also support development of immunoprevention strategies that could be implemented in people with an increased risk for glioma development. To this end, it will be critical to gain a better understanding of the etiology and risk factors for glioma development in humans. Although risk factors for gliomas are still largely unknown, patients with LGGs are known to be at extremely high risks for developing HGGs (34). Based on our data demonstrating that early-stage gliomas on day 21 resemble human LGGs (Supplementary Fig. S1) and that celecoxib treatment starting on day 21 is effective (Fig. 1B), patients with LGGs may benefit from celecoxib treatment to reduce the risk for malignant progression with HGGs.
With regard to novel risk factors for glioma, we recently reported that single-nucleotide polymorphisms (SNPs) in IFNAR1 and IFNA8 correlate with altered overall survival of patients with WHO grade 2–3 gliomas (10). Others have reported that SNPs in IL-4R and CX3CR1 correlate with survival of glioma patients (35–38). Although these data do not directly dictate risk factors for glioma development, it might be helpful to reveal functional significance of these SNPs in order to identify individuals who have a risk of gliomas and are likely to benefit from future clinical trials with NSAID-based immunoprevention. Based on the current study, future studies evaluating SNPs in Cox-2, Ccl2 as well as Cxcl10 in relation to glioma risks and prognosis are warranted.
This work was supported by the following grants: the National Institute of Health 1R01NS055140 (HO), 2P01NS40923 (HO), 1P01CA132714 (HO), and 1R21NS055738 (JRO); American Cancer Society RSG-09-189-01-LIB (JRO); Pittsburgh Foundation D2008-0433 (MF); the Cancer Center Support Grant P3CA047904 (UPCI).
We appreciate Dr. Axel H. Schönthal for providing us with demethylcelecoxib. We also thank Heather A. McDonald, Louis Delamarre, and Tara E. Hymes for their technical assistance.