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The Bcl-2 family member Bax translocates from the cytosol to mitochondria where it oligomerizes and permeabilizes the mitochondrial outer membrane to promote apoptosis. Bax activity is counteracted by pro-survival Bcl-2 proteins, but how they inhibit Bax remains controversial, because they neither co-localize nor form stable complexes with Bax. We constrained Bax in its native cytosolic conformation within cells using intramolecular disulfide tethers. Bax tethers disrupt interaction with Bcl-xL in detergents and cell free MOMP activity, but unexpectedly induce Bax accumulation on mitochondria. Fluorescence Loss in Photobleaching (FLIP) reveals constant retrotranslocation of wt Bax, but not tethered Bax, from the mitochondria into the cytoplasm of healthy cells. Bax retrotranslocation depends on pro-survival Bcl-2 family proteins and inhibition of retrotranslocation correlates with Bax accumulation on the mitochondria. We propose that Bcl-xL inhibits and maintains Bax in the cytosol by constant retrotranslocation of mitochondrial Bax.
Bcl-2 proteins control many pathways of programmed cell death in multi-cellular animals. Members of the Bcl-2 family can be grouped in pro-survival Bcl-2-like proteins and proapoptotic Bax-like members (Chipuk and Green, 2008; Cory and Adams, 2002; Youle and Strasser, 2008). The functions of Bcl-2 family members can be regulated by a diverse group of “BH3-only” proteins that initiate the pro-apoptotic activities of Bax-like proteins (Chipuk and Green, 2008).
Bax resides in the cytoplasm of healthy cells and translocates to the mitochondrial outer membrane (MOM) upon apoptosis induction (Wolter et al., 1997) where it causes cytochrome c (cyt c) release from the mitochondrial inter membrane space and mitochondrial dysfunctions (Gross et al., 1998; Martinou et al., 1999; Wang et al., 2001; Wei et al., 2001). The three concomitant events that characterize the commitment of a cell to apoptosis, Bax oligomerization, cyt c release and breakdown of the interconnected mitochondrial network, are tightly linked to the process of Bax translocation.
An early “rheostat model” proposed that Bax is restrained by heterodimerization with pro-survival Bcl-2 family proteins (Korsmeyer et al., 1993). However, this view could not be reconciled with experimental evidence of monomeric Bax residing in the cytoplasm of healthy cells, in contrast to the mitochondrial localization of Bcl-2 on the MOM (Hsu et al., 1997; Hsu and Youle, 1998). Although interactions between Bax and pro-survival Bcl-2 proteins control Bax activity (Fletcher et al., 2008) the question remains: How do prosurvival Bcl-2 proteins regulate Bax from a distance without interacting with Bax in the cytoplasm?
In an attempt to resolve the dilemma of Bax regulation by pro-survival Bcl-2 proteins independent of “sequestration”, BH3-only proteins have been suggested to mediate the link between cytosolic Bax and the mitochondrial pro-survival proteins. Some findings indicate that Bax can bind to and be activated by the BH3-only proteins Bim, Puma or the proapoptotic Bcl-2 family protein tBid (Desagher et al., 1999; Kim et al., 2006; Kuwana et al., 2005; Letai et al., 2002). Accordingly, these Bax “activator” proteins are proposed to be sequestered and neutralized by pro-survival Bcl-2 family members in healthy cells. In response to apoptosis induction “activator” proteins could be released from pro-survival Bcl-2 family proteins, perhaps by competition with other BH3-only proteins binding to prosurvival Bcl-2 family members, to activate Bax (Kim et al., 2006). Cell-free assays show a synergistic effect of tBid or Bim on Bax-mediated membrane permeabilization, suggesting a role of both proteins in direct Bax activation (Kuwana et al., 2005; Wei et al., 2000). Apoptosis assays with Bid/Bim DKO MEFs and the phenotypes of the corresponding knockout mice show that many apoptosis pathways do not depend on activity of either tBid or Bim (Willis et al., 2007), while the analysis of Bid/Bim/Puma TKO cells shows an effect on apoptosis induction by several stimuli (Ren et al., 2010). However, direct binding between Bax and BH3-only proteins in cells is not readily apparent (Walensky et al., 2006).
Further evidence indicates that Bax interacts with pro-survival Bcl-2 proteins and suggests that BH3-only proteins play a role in interfering with the heterodimer formation between Bax and pro-survival Bcl-2 proteins rather than directly activating Bax (Chen et al., 2005; Willis et al., 2005; Willis et al., 2007). Bax also has been found to undergo major conformational changes to integrate in lipid bilayers where membrane-bound Bax can form stable complexes with either tBid or Bcl-xL (Dlugosz et al., 2006; Lovell et al., 2008). However, the models of anti- and pro-apoptotic Bcl-2 family member interaction fail to explain why during apoptosis inhibition increased Bcl-xL concentrations do not result in an accumulation of Bax on mitochondria in complex with Bcl-xL.
We report here that Bax in the cytoplasm of non-apoptotic cells continually binds to mitochondria and retrotranslocates back to the cytoplasm through interaction with Bcl-xL. This indicates a new mechanism of anti-apoptotic Bcl-2 family member inhibition of Bax activation and apoptosis.
The activation of Bax involves major changes in its protein conformation that are linked to mitochondrial localization and integration into the MOM. We sought to hinder conformational changes involving α helices 1 and 2 of Bax containing the BH3 motif to analyze their involvement in Bax activity. To constrain Bax in its inactive conformation we substituted F30 and L63, which are in close proximity, to cysteine residues to form an intramolecular disulfide bond between α helices 1 and 2 (1–2, Figures 1A, S1A). We also changed E44 and P130 to cysteines to constrain the flexible loop between α helices 1 and 2 to the tip of helix 6 (L-6). In addition the intrinsic cysteine residues C62 and C126 were substituted by serine residues (Bax ΔSH) to avoid interference with the engineered disulfide bonds.
Previous reports have shown that disulfide bonds can form in the reducing environment of the cytosol (Bessette et al., 1999; Locker and Griffiths, 1999; Ostergaard et al., 2004; Schouten et al., 2002). We examined if the disulfide bonds 1–2 and L-6 are formed in Bax expressed in HCT116 Bax/Bak DKO cells by SDS-PAGE and Western blot in the absence and presence of β-mercapto-ethanol (BME). Wild-type Bax and the Bax variants C62S, C126S and C62/126S (ΔSH) migrate similarly with and without BME, while Bax variants with one or two engineered disulfide bonds (1–2, L-6 and 1–2/L-6) migrate faster in the absence of BME than wt Bax (Figure 1B). The decreased Stokes radius of the denatured Bax variants in the absence of BME indicates that the engineered disulfide bonds form in Bax within cells.
We confirmed the absence of free -SH groups in Bax 1–2/L-6 by thiol trapping using a maleimide derivative with a 10 kDa mPEG fusion (mPEG-MAL), while wt Bax becomes modified (Figure S1B). The analysis of Bax variants expressed in HCT116 Bax/Bak DKO cells with mPEG-MAL also showed free -SH groups in GFP-Bax wt that are absent in GFP-Bax ΔSH (Figure S1C). Thiol trapping of either GFP-Bax 1–2 or GFP-Bax L-6 shows pools of unmodified but also of modified protein, while GFP-Bax 1–2/L-6 remains unaltered, suggesting stabilization of a compact Bax fold by the two disulfide bonds thereby shielding the disulfides from the reducing environment of the cytosol. The redox potential of the disulfide bonds of this Bax variant was determined to be lower than −370 mV (Figure S1D) consistent with their formation in the cytosol (Schafer and Buettner, 2001).
We analyzed the conformation of recombinant Bax 1–2/L-6 by NMR in comparison to wt Bax. NMR chemical shift (δ) is sensitive to molecular conformation. Differences of chemical shifts (Δδ) between wt Bax and Bax 1–2/L-6 can be used as a probe of conformational differences of these two molecules. Noticeable differences in chemical shifts of the backbone amide proton and nitrogen are present, but are limited to the regions where mutations were introduced (Figure S1E). The absence of significant differences that are not associated to mutations indicates that the global structure of Bax 1–2/L-6 is essentially the same as that of wt Bax. In addition, Nuclear Overhauser Effect (NOE) is direct evidence of molecular structure as it reports two protons within 5Å. The NOE spectra from five tryptophan side chains were unaffected by the substitutions (Figure 1C). Noteworthy, the side chain Hε1 of Trp158 located at the loop between α6 and α7 helices showed NOEs to Hα and Hγ2 of Ile19 that is 11 residues away from the F30C mutation site, where both Ile19 and Cys30 are located within the α1 helix. In wt Bax, the same NOEs between Trp158 Hε1 and Ile19 Hγ2 and Hα were observed. We also found the regions of flexibility of Bax 1–2/L-6 are the same as wt Bax only differing with reduced dynamics at the L-6 disulfide tether (Figure S1F). Thus the intramolecular tethers stabilize the native and inactive conformation in Bax 1–2/L-6 that is similar to inactive wt Bax (Note S1).
We tested the influence of stabilizing the inactive conformation of Bax in cells by measuring caspase 3/7 activity. Staurosporine (STS)-induced caspase activity in HCT116 Bax/Bak DKO cells expressing Bax ΔSH is similar to wt Bax expressing cells (data not shown) and prevented by Bcl-xL over-expression (Figure 2A). In parallel to the caspase activity assay in Bax ΔSH-expressing cells STS induces increased cyt c release and cell death indicated by the release of LDH that is inhibited by Bcl-xL over-expression. Similar activities were obtained in HCT116 Bax KO cells with Bax ΔSH or additional single cysteine substitution of either F30, E44, L63 or P130 showing that the substitutions used in Bax 1–2/L-6 do not interfere with Bax activity without disulfide bond formation (data not shown). In all three assays Bax 1–2/L-6 lacks STS-inducible activity (Figure 2A–C). However, in the presence of Bcl-xL (Figure 2B) or in the absence of apoptosis induction (Figure S2A, B) over-expression of Bax 1–2/L-6 induced cyt c release more than over-expression of wt Bax. The ability of recombinant Bax 1–2/L-6 to induce cyt c release was also tested using mitochondria isolated from Bax/Bak DKO MEFs (Figure 2D). In this assay recombinant wt Bax causes the release of cyt c from isolated mitochondria in the presence of tBid. Recombinant Bax 1–2/L-6 fails to induce cyt c release even in the presence of tBid. Thus, the intramolecular tethers in Bax 1–2/L-6 decrease its activation by BH3-only proteins and regulation by Bcl-xL. Although Bax and Bcl-xL do not interact in the cytoplasm of cells their interaction can be induced in vitro by detergents (Hsu and Youle, 1997). We assessed if constraining Bax with intramolecular tethers interferes with this interaction. While wt Bax and Bcl-xL interact in the presence of different detergents at concentrations above CMC, Bax 1–2/L-6 forms heterodimers with Bcl-xL only in Triton X100, Triton X114 and dodecyl maltoside (Figure S2C, Note S2). Thus, intramolecular tethers can interfere with detergent-induced Bcl-xL binding.
Inactive Bax resides mainly in the cytoplasm. Upon activation Bax forms foci at the tips and constriction sites of mitochondria that is temporally associated with MOMP and cyt c release (Karbowski et al., 2004). Like wt Bax, Bax ΔSH is found predominantly in the cytosol of transfected HCT116 Bax/Bak DKO cells and translocates to mitochondria upon apoptosis stimulation (Figures 3A–C). Surprisingly, Bax 1–2/L-6 is not located in the cytosol and smoothly coats the mitochondria in 99 % of healthy cells and remains unchanged in the presence of apoptotic stimuli (Figure 3A–C). Whereas Bcl-xL over-expression prevents the localization of Bax ΔSH to the mitochondria after apoptosis induction, GFP-Bax 1–2/L-6 circumscribes the mitochondria even on Bcl-xL over-expression (Figure S3 A, Note S3). Cell fractionation confirms that in contrast to Bax ΔSH, most Bax 1–2/L-6 is found in the heavy membrane (HM) fraction in the absence of apoptosis induction (Figure 3D). Tethered Bax is largely carbonate extractable, suggesting that it binds mitochondria but does not integrate into the MOM.
Why does tethered Bax localize to mitochondria in healthy cells despite adopting an inactive conformation? Although wt Bax resides mainly in the cytoplasm of healthy cells, a fraction localizes to mitochondria but, in contrast to mitochondrially embedded Bax found following apoptosis induction, is carbonate extractable (Desagher et al., 1999). We hypothesized that the mitochondrial Bax pool could be in equilibrium with cytosolic Bax in healthy cells, which could be disrupted by Bax tethers. In an attempt to distinguish between cytosolic and mitochondrial Bax and compare wt Bax with Bax 1–2/L-6, we performed fluorescence loss in photobleaching (FLIP) with different GFP-Bax variants expressed in HCT116 Bax/Bak DKO cells. To this end, we repeatedly bleached a region in the nucleus of a transfected cell (Figure 4A, white square). The declining GFP fluorescence in the targeted cell was followed by assigning regions of interest in the cytoplasm (green circle) and on the mitochondria (red and blue circles) in Figure 4A. GFP-Bax readily crosses the nuclear envelope and cytosolic GFP fluorescence of the targeted cell was bleached rapidly by FLIP, whereas the neighboring reference cell fluorescence (black circle) remained stable ruling out photo bleaching during imaging (Figure 4A, B). After reducing the cytosolic GFP-Bax signal the mitochondrial GFP-Bax pool was readily apparent (Figure 4B, arrows). The decay of mitochondrial GFP-Bax fluorescence by FLIP occurs within 660 s following a first order kinetic at a rate (4.7 ± 0.1 × 10−3s−1) notably slower than the loss in cytosolic fluorescence (Figures 4B, C, S4A). Interestingly, Bcl-xL over-expression causes more than an 80 % increase in the rate of mitochondrial fluorescence reduction during FLIP at comparable levels of Bax expression (Figure 4B–E). The loss in mitochondrial GFP-Bax fluorescence during FLIP suggests that Bax could exist in an equilibrium between mitochondrial and cytosolic states (Figure 4D).
The presence of MG132 had no effect on GFP-Bax fluorescence loss with or without Bcl-xL (Figure S4B), indicating that proteasomal degradation does not account for the decrease in mitochondrial fluorescence during FLIP. To directly assess Bax return to the cytosol from mitochondria, we analyzed fluorescence recovery after photobleaching (FRAP) of cytosolic GFP-Bax (Figure S4C). Following the bleach GFP-Bax fluorescence increases in the cytosol by about 25 % after 400 s following a first-order kinetic (Figure 4F). Over-expression of Bcl-xL increases the cytosolic reappearance of GFP-Bax fluorescence more than 2-fold, when mitochondrial post-bleach GFP-Bax levels were comparable (Figure S4D). We examined whether continual retrotranslocation is balanced by continual binding of Bax to mitochondria in healthy cells. By photobleaching half of a cell expressing GFP-Bax (Figure 4H) we quantified the binding of Bax to mitochondria over the subsequent 10 minutes (Figure 4G, H, S4E). Bax wt translocates to mitochondria at a rate of 4.7 ± 0.2 × 10−3s−1, consistent with an equilibrium between on and off rate.
Although FLIP analyses appear to measure an increase in mitochondrial Bax off rates by Bcl-xL, it could be suggested that wt Bax and Bcl-xL may compete for the same binding site on the mitochondria causing increased Bax retrotranslocation into the cytoplasm. This possibility was tested by analyzing the effect of untagged Bax over-expression on GFP-Bax retrotranslocation (Figure S4F). In contrast to Bcl-xL over-expression Bax slightly decreases the GFP-Bax retrotranslocation rate (4.4 ± 0.1 × 10−3s−1), indicating no competition between Bax and Bcl-xL for MOM binding. In the presence of untagged Bax the over-expression of Bcl-xL accelerates GFP-Bax retrotranslocation (7.0 ± 0.2 × 10−3s−1), but significantly less than without untagged Bax (8.6 ± 0.4 × 10−3s−1), suggesting Bax can compete with GFP-Bax for Bcl-xL mediated retrotranslocation. Mitochondrial Bax retrotranslocation into the cytoplasm dependent on the Bcl-xL concentration may provide a rationale for the mitochondrial accumulation of Bax 1–2/L-6. The intramolecular tethers in Bax 1–2/L-6 may interfere with Bcl-xL-mediated retrotranslocation, as they also disrupt the interaction between Bax and Bcl-xL in some detergents. We applied FLIP to analyze Bax 1–2/L-6 retrotranslocation, bleaching the low GFP-Bax 1–2/L-6 fluorescence in the cytoplasm as was done for wt GFP-Bax. Mitochondrial GFP-Bax 1–2/L-6 fluorescence intensity was not significantly reduced by repeated bleaching (Figure 5A, B). In contrast to wt Bax, Bcl-xL over-expression did not detectably increase the retrotranslocation of Bax 1–2/L-6 in a 660 s time frame (Figure 5A, B). Thus, Bax 1–2/L-6 is deficient in retrotranslocation. We examined the role of helix 9 in Bax 1–2/L-6 binding to mitochondria. Bax 1–2/L-6 displayed the same sensitivity to S184 mutations as wt Bax (Nechushtan et al., 1999) (Figure S5A, B), indicating that helix 9 is required for Bax 1–2/L-6 binding to mitochondria.
We tested the effect of different Bcl-2 family members on Bax retrotranslocation. Over-expression of Bcl-2 and Mcl-1 accelerated Bax retrotranslocation similarly to Bcl-xL (Figure 6A, S6A). In contrast, the BH3-only protein Bim reduced the rate of Bax retrotranslocation more than 3 fold to 1.3 ± 0.2 × 10−3s−1 in HCT116 Bax/Bak DKO cells that did not contain Bax foci. Endogenous Bak expression tested by comparing HCT116 Bax/Bak DKO and Bax KO cells has no influence on Bax retrotranslocation (Figure 6A, S6A). After MOMP or in the presence of the viral Bax inhibitor vMIA (Arnoult et al., 2004) wt Bax retrotranslocation is inhibited (Note S4, Figure S6B–E).
To analyze if binding of pro-survival Bcl-2 proteins to Bax is required to mediate Bax retrotranslocation, we examined Bcl-xL G138A, a variant that is deficient in Bax binding and apoptosis inhibition (Desagher et al., 1999; Dlugosz et al., 2006; Sedlak et al., 1995). In contrast to wt Bcl-xL, G138A failed to accelerate retrotranslocation of GFP-Bax when expressed at levels comparable to wt Bcl-xL (Figure 6B, C). Furthermore, the Bcl-2/Bcl-xL inhibitor ABT-737 (Oltersdorf et al., 2005) reduced the rate of Bax retrotranslocation by over 75%, suggesting that endogenous Bcl-2 family members mediate Bax retrotranslocation (Figure 6D, S7A). These results indicate the involvement of direct interactions between prosurvival Bcl-2 proteins and Bax for retrotranslocation.
The Bax variant D68R has been previously shown to exhibit insensitivity towards Bcl-2/Bcl-xL inhibition and potent pro-apoptotic activity (Fletcher et al., 2008). Interestingly, Bax D68R constitutively localizes to the mitochondria of HCT116 Bax/Bak DKO cells in the absence of apoptosis stimuli (Figure 6E, F). Bax D68R localizes to the mitochondria even in cells not displaying cyt c release (Figure 6F). We analyzed whether Bax D68R retrotranslocation could be accelerated by over-expression of the pro-survival Bcl-2 proteins Bcl-2, Bcl-xL and Mcl-1. Bax D68R retrotranslocates at less than ½ the rate of wt Bax (Figure 6G), while the S184V substitution in helix 9, that also increases the mitochondrial Bax pool, only slightly decreases Bax retrotranslocation (Note S5, Figure S7B, C). In contrast to wt Bax, the retrotranslocation rate of D68R is only slightly increased by Bcl-2 and Bcl-xL over-expression from 2.1 ± 0.1 × 10−3s−1 to about 3.9 × 10−3s−1, while over-expression of Mcl-1 does not accelerate Bax D68R retrotranslocation (Figure 6G). The ability of the different prosurvival Bcl-2 proteins to increase Bax D68R retrotranslocation correlates with the relative affinities of Mcl-1, Bcl-2 and Bcl-xL for Bax D68R (Fletcher et al., 2008). The diminished retrotranslocation of Bax D68R extends the results obtained with tethered Bax 1–2/L-6 indicating the importance of pro-survival Bcl-2 protein interactions with the BH3 domain of Bax, which is further indicated by the retrotranslocation of a Bcl-xL chimera with its helices 2 and 3 replaced by the corresponding Bax helices (George et al., 2007). The retrotranslocation rate of this chimera is similar to the rate of Bax (Figure S7D).
The possibility of Bcl-xL retrotranslocation was analyzed by performing FLIP with HCT116 Bax/Bak DKO cells expressing GFP-Bcl-xL. GFP-Bcl-xL localizes in these cells predominantly to the mitochondria (Figure S7E) and retrotranslocates in the absence of Bax with a low rate (1.4 ± 0.1 × 10−3s−1) from the mitochondria into the cytoplasm (Figure 6H). Over-expression of Bax accelerates Bcl-xL retrotranslocation about 3.5-fold suggesting they interact on mitochondria, retrotranslocate together and dissociate in the cytosol. Interestingly, ABT-737 increases the Bcl-xL retrotranslocation rate (Figure S7F).
Upon translocation to the mitochondria during apoptosis wt Bax exposes an epitope consisting of P13-I19 at the N-terminus of helix 1 for the monoclonal antibody 6A7 that is not accessible in cytosolic and mitochondrial wt Bax in healthy cells (Hsu and Youle, 1998). This change in the 6A7 epitope correlates with foci formation and cyt c release (Nechushtan et al., 1999). Despite constitutive mitochondrial localization Bax 1–2/L-6 fails to form foci.
Surprisingly, Bax 1–2/L-6 is 6A7-positive in some but not all cells (Figure 7A, B), while circumscribing the mitochondria (Figure 3A). Only a subset of Bax 1–2/L-6 on the mitochondria adopts a 6A7-positive fold as inferred by the Pearson’s coefficient of about 0.7 (Figure 7C). The pool of 6A7-positive cells transfected with Bax 1–2/L-6 is slightly decreased by Bcl-xL over-expression, whereas almost 100 % of wt Bax-expressing cells are 6A7-negative with Bcl-xL over-expression (Figure 7B). Interestingly, Bax 1–2/L-6 changes to its 6A7-positive conformation gradually over 24 hours on the mitochondria of healthy cells (Figure 7D).
Although the disulfide tethers in Bax 1–2/L-6 would reduce the conformational flexibility of its N-terminal part, they do not completely block Bax from undergoing a conformational change on the mitochondria that results in the exposure of the 6A7 epitope. Since Bax 1–2/L-6 does not show induced apoptotic activity, the 6A7-positive conformational change smoothly coating mitochondria seems to be an intermediate step en route to activation likely correlating with spontaneous induction of cyt c release (Figure S2 A, B) upstream of foci formation. As wt Bax does not reach the 6A7-positive state when circumscribing mitochondria in healthy cells the prolonged association of Bax 1–2/L-6 with mitochondria may be the stimulus. Subsequent conformational rearrangements inhibited by the tethers likely are associated with foci formation.
Pro-survival Bcl-2 proteins prevent apoptosis by inhibiting Bax and Bak. They block Bax translocation from the cytosol to the mitochondria, Bax oligomerization and MOMP. Paradoxically, pro-survival Bcl-2 proteins on the mitochondria stabilize Bax localization in the cytosol, without forming stable heterodimeric complexes. Bax regulation by Bcl-2 thus creates a spatial paradox that has been addressed by previous models of Bax activation (Kim et al., 2006; Willis et al., 2007).
We propose a new model of continuous Bax retrotranslocation from mitochondria that is consistent with results from numerous labs. We find that Bax translocates constantly to the mitochondria in healthy cells, where pro-survival Bcl-2 proteins, such as Bcl-xL, bind Bax and retrotranslocate it back into the cytoplasm thereby stabilizing the inactive Bax conformation (Figure 7E, F). Bcl-xL and Bax both retrotranslocate from mitochondria and accelerate the rate of each other’s retrotranslocation after transient interaction on mitochondria, perhaps through trans-sequestration of the C-terminal tails (Jeong et al., 2004). Evidence for direct interaction is based on the inhibition of Bax retrotranslocation when the Bax-Bcl-xL binding is disrupted by i) the G138A mutation in the hydrophobic groove of Bcl-xL (Sedlak et al., 1995), ii) the D68R mutation in the BH3 domain of Bax (Fletcher et al., 2008), and iii) the Bcl-xL inhibitor ABT-737 (Oltersdorf et al., 2005). The interaction between Bax and Bcl-xL requires prior conformational changes in the N-terminal part of Bax because preventing these conformational changes by intramolecular tethers disrupts interaction with Bcl-xL in detergents and Bax retrotranslocation.
The absence of retrotranslocation results in Bax 1–2/L-6 accumulation on the mitochondria in healthy cells. Wild-type Bax, however, only accumulates on mitochondria when the activities of pro-survival Bcl-2 proteins are blocked by BH3-only proteins, such as Bim, or by ABT-737. Bax accumulated on mitochondria upstream of MOMP can dissipate by retrotranslocation if pro-survival Bcl-2 proteins become available again, as observed when cells reattach to substrate following transient anoikis (Gilmore et al., 2000).
Conformational changes of Bax on the mitochondria during apoptosis involve the N-terminus of Bax and can be detected using the monoclonal antibody 6A7. Despite its reduced apoptotic activity tethered Bax eventually adopts a 6A7-positive fold, but does not form mitochondrial foci. Although in cell free assays tethered Bax completely lacks tBID activated MOMP, consistent with the lack of apoptosis induced activation in cells, tethered Bax can spontaneously induce some degree of MOMP within cells even in the presence of Bcl-xL, likely though this 6A7 positive form. Since the 6A7 antibody can compete for Bcl-xL binding to Bax (Hsu and Youle, 1998), a 6A7-positive conformation of wt Bax may normally exist circumscribing mitochondria that remains undetectable because 6A7 binding is sterically blocked by Bcl-xL bound to Bax. Bax conformational changes in α helices 1 and 2 could be a normal consequence of Bax binding to the mitochondria perhaps stimulated by lipid interactions (Kuwana et al., 2002). If not retrotranslocated, mitochondrial wt Bax becomes active due to further conformational changes and oligomerization to cause MOMP (Figure 7F).
In addition to a reduced Bax retrotranslocation (off rate), mitochondrial Bax accumulation could also result from an increase in the Bax translocation (on rate), which may depend on direct Bax activation by BH3-only proteins (Kuwana et al., 2005). Even the steady state binding of Bax to mitochondria in healthy cells may result from the activity of residual levels of BH3-only proteins in healthy cells. Bax binding to the MOM appears to be influenced by the exposure of the C-terminal membrane anchor (Gavathiotis et al., 2010), which may also depend on isomerisation of the prolyl bond preceding P168 and its acceleration by the PPIase Pin1 (Shen et al., 2009). Bax translocation to the MOM, however, seems not to be influenced by Bcl-xL.
Despite the robust interaction of Bax and Bcl-xL in detergents (Hsu and Youle, 1998) and in membranes (Dlugosz et al., 2006), increased concentrations of pro-survival mitochondrial bound Bcl-2 proteins in cells do not result in Bax accumulation on mitochondria. In contrast, Bax can be directly bound and inhibited by the viral protein vMIA that accumulates Bax on the mitochondria as it inhibits apoptosis (Arnoult et al., 2004). In healthy cells the sub-cellular location of Bax depends on constant retrotranslocation of mitochondrial Bax into the cytosol by pro-survival Bcl-2 proteins. Minimization of a mitochondrial Bax pool that is susceptible for activation is likely to prevent apoptosis and explains the spatial paradox of Bcl-2 protein inhibition of Bax.
HCT116 cells were cultured in McCoy's 5A medium supplemented with 10% heat-inactivated fetal bovine serum and 10 mM Hepes in 5% CO2 at 37°C. HCT116 Bax/Bak DKO cells were obtained by deletion of the Bak gene by homologous recombination in the HCT116 Bax −/− cells (Wang and Youle, manuscript in preparation). Cells were transfected with PolyJet (SignaGen) or Lipofectamine LTX (Invitrogen) typically with 100 ng of the GFP-Bax construct according to the manufacturer's instructions and cells were incubated for 6–8 hours for confocal imaging. For Western blot cells were harvested 8 hours after transfection.
HCT116 Bax/Bak DKO cells were seeded on a chambered coverglass (Thermo scientific) in McCoy's 5A medium (10 mM Hepes), grown for 20 hours, transfected and incubated for 6–8 hours. The cells were then fixed with 4% paraformaldehyde solution for 10 minutes and washed with PBS. The fixed cells were permeabilized with Triton X100 for 15 minutes at room temperature. For double immunofluorescence staining, cells were first incubated with 5% BSA in PBS for 1 hour at room temperature, followed by incubation with appropriate primary antibodies (anti-Tom20 and anti-6A7 antibodies) in 5% BSA solution for 2 h and probed with an Alexa-594 an Alexa-647-conjugated secondary antibody (Invitrogen). Confocal analysis was performed on a Zeiss 510 META confocal LSM microscope equipped with argon (458/488/514 nm lines) and HeNe (543/633 nm) lasers. For experiments measuring the recovery after photobleaching (FRAP), one ROI within the nucleus of a cell of interest was photobleached with the argon laser at 100 % intensity. Recovery of fluorescence in the cytoplasm was monitored immediately after photobleaching by imaging the bleached cell in 20-s intervals with low laser intensity (1%). The results were normalized setting the starting fluorescence to 100 % signal.
For Bax translocation assay the cells were incubated with mitotracker-far red for 10 min. prior analysis. Approximately half of an analyzed cell was bleached with high laser power (100 %) for 17.5 ms. After either 1, 2, 4 or 10 min. the cytoplasm of the cell was bleached a second time for 25 ms with high laser power. After the bleaching two different ROI each were assigned for unbleached and bleached mitochondria.
In fluorescence loss in photobleaching (FLIP) experiments, a single spot with a diameter of 1 µm within the nucleus was repeatedly bleached with two iterations of 100% power of a 488 nm laser line (100% output) using a Zeiss LSM510 META with 63X PlanFluor lens. The average diameter of a single z-axis plane varied between 2–2.5 µm. Two images were collected after each bleach pulse, with 30 s between bleach pulses. After collecting 30 images, two separate measurements on the mitochondria were taken to analyze the fluorescence loss. Unbleached control cells were monitored for photobleaching due to image acquisition. The rate of loss in fluorescence on the mitochondria was calculated from fluorescence intensity measurements using the Zeiss LSM software. The fluorescence intensities were normalized by setting the initial fluorescence to 100% signal. Plots are shown as normalized fluorescence over time.
For caspase 3/7 measurementsHCT116 Bax/Bak DKO cells were transfected with different Bax constructs in 96-well plates and incubated with or without 1 µM STS for 4 h. Then Apo-ONE® Caspase-3/7 Reagent (Promega) was added according to manufacturer’s protocol. The samples were incubated for 16 h in the dark and then analyzed by measuring the fluorescence with an excitation wavelength of 488 nm and an emission wavelength range of 530 nm. For LDH measurements 96-well plates with HCT116 Bax/Bak DKO cells transfected with different Bax constructs were incubated with 1 µM STS for 24 h. Then 50µl of the supernatant from each well were transferred in a new plate and 50 µl of the substrate mix (Promega, cytotox 96 kit) was added to each well of the plate. After 30–60 min 50 µl of stop solution was added and the absorbance at 490 nm was detected.
We thank Dr. D. W. Andrews and Dr. J.-C. Martinou for helpful discussions and comments. We also thank Dr. D. R. Green for the Omi-mCherry construct. This work is supported by the Leopoldina, National Academy of Sciences, Germany (to FE), La Ligue contre le Cancer (to DA) the NHLBI (MS & NT) and the NINDS intramural programs.
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