PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of jcinvestThe Journal of Clinical InvestigationCurrent IssueArchiveSubscriptionAbout the Journal
 
J Clin Invest. 2011 April 1; 121(4): 1251–1253.
Published online 2011 March 23. doi:  10.1172/JCI57211
PMCID: PMC3069797

Myocarditis: a defect in central immune tolerance?

Abstract

Myocarditis, or inflammation of the heart, is a potentially devastating disease that can result from both viral infection and autoimmune attack of self antigens in the heart. In the current issue of the JCI, Lv and colleagues use a genetically susceptible mouse model to show that myocarditis is a T cell–mediated autoimmune disease that occurs due to insufficient thymic negative selection of α-myosin–reactive T cells.

Myocarditis is a poorly understood disease

Myocarditis is a disease characterized by the presence of inflammatory infiltrates in heart tissue, which can lead to dilated myocardiopathy and ultimately to congestive heart failure (1). Both viral and nonviral infections, such as those of coxsackie virus B and Trypanosoma cruzi, respectively, are known to cause myocarditis (2). However, for a subset of patients with the disease, common causal infections are undetectable, but autoantibodies against cardiac antigens are present and symptoms improve following immunosuppressive treatments. Nevertheless, the physiological distinction between these two forms of myocarditis may be blurry, as some evidence supports a transition from a virally instigated heart inflammation to activation of the immune system against self antigens. Treatments for myocarditis remain insufficient, and further work toward identifying causes of myocarditis could help increase options. For example, identification of the relative importance of autoantigens in the more autoimmune forms of the disease could lead to antigen-specific tolerogenic therapies. Furthermore, the best diagnostic tool for myocarditis remains an invasive biopsy, so a better understanding of disease etiology could help guide less invasive diagnostic procedures.

A spontaneous model of myocarditis

The NOD mouse contains many genetic susceptibility loci that cause it to spontaneously develop autoimmune diabetes in a T cell–dependent manner (3). In 2003, it was found that replacement of a specific allele of the MHC class II complex (I-Ag7) with a human DQ8 allele (DQ8+NOD mice) led to protection from NOD autoimmune diabetes, but also resulted in a predisposition to myocarditis and dilated cardiomyopathy (4). Paradoxically, the human DQ8 allele had previously been associated with type 1 diabetes. Nevertheless, further investigation showed that dilated cardiomyopathy in these mice was a result of CD4+ T cell activity and that autoantibodies alone were insufficient to account for the observed disease (5, 6). Thus, while the DQ8+NOD mouse strain is a valuable model for studying T cell–mediated autoimmunity arising from multiple dysfunctional regulatory pathways, the mechanism directly responsible for disease development in these mice remained unknown.

In the current issue of JCI, Lv et al. demonstrated that a defect in thymic negative selection, the deletion of autoreactive T cells during their development, is responsible for the spontaneous myocarditis observed in DQ8+NOD mice. The authors showed that the presence of target antigen for α-myosin–specific T cells in the thymus prevented their functional maturation and attack of cardiac tissue (7). First, the authors showed that it is the cardiac-specific α-myosin isoform, and not the skeletal muscle β-myosin isoform, that appears as the first autoantibody target in DQ8+NOD mice, consistent with the heart-directed autoimmunity observed in these mice. They also demonstrated that T cells specific for α-myosin could be isolated from heart infiltrates, and that such T cells displayed a potentially pathogenic Th1 cytokine profile. Next, the authors examined the B and T cell dependence of DQ8+NOD myocarditis using mice selectively deficient in either cell type, and found that while T cells are critical for disease to occur, myocarditis could occur in the absence of B cells, albeit at a slower rate. This mirrors the relative T and B cell dependence of autoimmune diabetes development in NOD mice and is consistent with autoimmune disease resulting primarily from defective tolerance among T cells (3). Interestingly, this finding also shares similarities to observations of the Aire-knockout mouse, in which defective negative selection of autoreactive T cells in the thymus leads to multiorgan autoimmune disease mediated exclusively by T cells (8).

Thymic selection prevents myocarditis in DQ8+NOD mice

Having provided evidence for the importance of α-myosin as a T cell antigen in DQ8+NOD myocarditis, Lv et al. went on to examine whether defective thymic selection and escape of α-myosin–reactive T cell clones from the thymus might explain why DQ8+NOD mice develop myocarditis (7). After finding that α-myosin mRNA seemed to be nearly undetectable in medullary thymic epithelial cells (mTECs) by examining published array data, the authors confirmed by quantitative PCR that α-myosin is unique among examined cardiac antigens in its absence from the thymic stroma. Suspecting that this lack of thymic antigen allowed for the development of α-myosin–reactive T cells and subsequent heart infiltration in DQ8+NOD mice, the authors transgenically introduced α-myosin into the thymus of DQ8+NOD mice and observed complete protection from the development of myocarditis (Figure (Figure1).1). Furthermore, they found that peripheral T cells from mice with thymic α-myosin remained unresponsive to the antigen ex vivo, which, taken together with the prevention from myocarditis, showed that the thymic antigen effectively tolerizes DQ8+NOD mice against α-myosin–directed autoimmunity. Last, the authors suggested that central tolerance to α-myosin may be ineffective in humans. They demonstrated that expression of α-myosin is near the minimum level of detection in stromal cells of the human thymus, meaning that it may be of little functional consequence. Ex vivo assays with peripheral blood mononuclear leukocytes demonstrated that while control patients exhibited some responsiveness to α-myosin even in the absence of disease, the response was markedly increased in patients with inflammatory heart disease. As relatively few patient samples were analyzed, further study is warranted to determine whether such assays will be broadly applicable to patients with myocarditis.

Figure 1
Permissive negative selection leads to myocarditis in DQ8+NOD mice.

The mechanism remains unclear

The findings of this article clearly demonstrate the importance of central tolerance in preventing autoreactive T cell development and subsequent autoimmunity, and provide another example of the value of studying models of spontaneous autoimmunity to identify autoantigens with clinical relevance (9). However, a lack of central tolerance to α-myosin in DQ8+NOD mice does not entirely explain the unique susceptibility of this mouse strain to myocarditis. WT NOD mice also lack α-myosin expression in the thymus, but, in these mice, the resulting lack of negative selection and release of α-myosin–reactive T cells to the periphery do not result in myocarditis. Therefore, the underlying cause of myocarditis induction in DQ8+NOD is likely to be peculiarities with the binding of α-myosin–derived peptides to the introduced DQ8 MHC allele, which might affect processes that are sufficient to mediate tolerance in WT NOD mice. Also, despite the absence of substantial α-myosin transcripts among thymic stroma, central tolerance to this antigen may still be physiologically relevant in DQ8+NOD mice, as recirculating dendritic cells, which pick up antigen in the periphery and then traffic to the thymus, have been shown to be an effective source of antigen for negative selection (10). When T cells encounter their antigen in peripheral tissues under non-inflammatory conditions, they are likely to be tolerized, so encounter of α-myosin–specific T cells with their antigen in heart-draining lymph nodes is a probable mechanism of tolerance in WT NOD mice. However, these tolerance processes are sensitive to the strength of signal delivered by antigen-MHC complexes, so differences in antigen binding and presentation by the DQ8 allele might perturb this mechanism of tolerance. Similarly, the introduced DQ8 allele is expressed as a transgene, as opposed to a knock-in, and, as such, may be expressed at levels that differ from those of endogenously expressed MHC class II complexes in WT NOD mice. Finally, the mechanism by which central tolerance is established in mice expressing transgenic thymic antigen is unclear, as negative selection of autoreactive T cells and induction of immunosuppressive regulatory T cells might both contribute to disease protection in this experimental system.

Future directions

The findings of the article by Lv et al. (7) demonstrate that, at least in DQ8+NOD mice, α-myosin is the earliest detectable target of autoantibodies in myocarditis, suggesting that it is likely an important initiating autoantigen for the autoimmune form of the disease. Further work is warranted to determine whether this autoantigen may have good early predictive value of future autoimmune cardiomyopathy in clinical settings. Also, given the ability of α-myosin–directed central tolerance to completely protect mice from myocarditis, future therapeutic approaches in the clinical arena may seek to induce T cell tolerance to this antigen.

Acknowledgments

This work was supported by the Juvenile Diabetes Research Foundation (JDRF), NIH, Leona M. and Harry B. Helmsley Charitable Trust, and Burroughs Wellcome Fund. We would also like to thank Kellsey Johannes and Imran Khan for helpful comments on a draft of this commentary.

Footnotes

Conflict of interest: The authors have declared that no conflict of interest exists.

Citation for this article: J Clin Invest. 2011;121(4):1251–1253. doi:10.1172/JCI57211

See the related article beginning on page 1561.

References

1. Cooper LT. Myocarditis. N Engl J Med. 2009;360(15):1526–1538. [PubMed]
2. Rose NR. Myocarditis: infection vs. autoimmunity. J Clin Immunol. 2009;29(6):730–737. doi: 10.1007/s10875-009-9339-z. [PubMed] [Cross Ref]
3. Anderson MS, Bluestone JA. The NOD mouse: a model of immune dysregulation. Annu Rev Immunol. 2005;23:447–485. doi: 10.1146/annurev.immunol.23.021704.115643. [PubMed] [Cross Ref]
4. Elliott JF, et al. Autoimmune cardiomyopathy and heart block develop spontaneously in HLA-DQ8 transgenic IAbeta knockout NOD mice. Proc Natl Acad Sci U S A. 2003;100(23):13447–13452. doi: 10.1073/pnas.2235552100. [PubMed] [Cross Ref]
5. Taylor JA, Havari E, McInerney MF, Bronson R, Wucherpfennig KW, Lipes MA. A spontaneous model for autoimmune myocarditis using the human MHC molecule HLA-DQ8. J Immunol. 2004;172(4):2651–2658. [PubMed]
6. Hayward SL, Bautista-Lopez N, Suzuki K, Atrazhev A, Dickie P, Elliott JF. CD4 T cells play major effector role and CD8 T cells initiating role in spontaneous autoimmune myocarditis of HLA-DQ8 transgenic IAb knockout nonobese diabetic mice. J Immunol. 2006;176(12):7715–7725. [PubMed]
7. Lv H, et al. Impaired thymic tolerance to alpha- myosin directs autoimmunity to the heart in mice and humans. J Clin Invest. 2011;121(4):1561–1573. [PMC free article] [PubMed]
8. DeVoss JJ, et al. Effector mechanisms of the autoimmune syndrome in the murine model of autoimmune polyglandular syndrome type 1. J Immunol. 2008;181(6):4072–4079. [PMC free article] [PubMed]
9. Shum AK, et al. Identification of an autoantigen demonstrates a link between interstitial lung disease and a defect in central tolerance. Sci Transl Med. 2009;1(9):9ra20. [PMC free article] [PubMed]
10. Bonasio R, Scimone ML, Schaerli P, Grabie N, Lichtman AH, von Andrian UH. Clonal deletion of thymocytes by circulating dendritic cells homing to the thymus. Nat Immunol. 2006;7(10):1092–1100. [PubMed]

Articles from The Journal of Clinical Investigation are provided here courtesy of American Society for Clinical Investigation