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Logo of jcinvestThe Journal of Clinical Investigation
J Clin Invest. 2011 April 1; 121(4): 1424–1428.
Published online 2011 March 1. doi:  10.1172/JCI46229
PMCID: PMC3069789

Rapid, reversible activation of AgRP neurons drives feeding behavior in mice


Several different neuronal populations are involved in regulating energy homeostasis. Among these, agouti-related protein (AgRP) neurons are thought to promote feeding and weight gain; however, the evidence supporting this view is incomplete. Using designer receptors exclusively activated by designer drugs (DREADD) technology to provide specific and reversible regulation of neuronal activity in mice, we have demonstrated that acute activation of AgRP neurons rapidly and dramatically induces feeding, reduces energy expenditure, and ultimately increases fat stores. All these effects returned to baseline after stimulation was withdrawn. In contrast, inhibiting AgRP neuronal activity in hungry mice reduced food intake. Together, these findings demonstrate that AgRP neuron activity is both necessary and sufficient for feeding. Of interest, activating AgRP neurons potently increased motivation for feeding and also drove intense food-seeking behavior, demonstrating that AgRP neurons engage brain sites controlling multiple levels of feeding behavior. Due to its ease of use and suitability for both acute and chronic regulation, DREADD technology is ideally suited for investigating the neural circuits hypothesized to regulate energy balance.


Obesity is an epidemic that continues to grow unabated in developed nations where an abundance of calorically dense foods are plentiful and easily obtained. This excess of food is a major contributor to high levels of obesity, which is a major risk factor for cardiovascular disease, certain types of cancer, and type 2 diabetes. Through neuroscience research, critical contributions can be made in developing an understanding of the mechanisms underlying this problem, ultimately leading to treatment of this escalating disorder (1).

Agouti-related protein (AgRP) neurons are located in the arcuate nucleus (ARC) of the hypothalamus and have been implicated in regulating energy homeostasis (2). They release AgRP, neuropeptide Y (NPY) and γ-aminobutyric acid (GABA), all of which have been linked with changes in energy balance (25). Despite evidence for their involvement in feeding behavior and energy balance, the exact contribution of these neurons remains unclear, since chronic and acute perturbations of AgRP neurons and the factors they release have produced inconsistent results. Overexpression of AgRP in transgenic mice produces obesity, and central administration of the orexigenic peptides, AgRP or NPY, causes hyperphagia (2, 3). However, targeted deletion of the genes encoding AgRP, NPY, and/or vesicular GABA transporter (VGAT; required for release of GABA) has minimal effects on feeding (46). Likewise, acute ablation of AgRP neurons in neonates or delayed degeneration of AgRP neurons during adulthood has little impact on energy metabolism (79). Conversely, acute ablation of AgRP neurons in adult mice, a manipulation associated with gliosis (5), induces severe hypophagia (7, 8). Given the above, manipulating AgRP neurons in a manner that is selective, rapid, and reversible, should provide insight, both qualitative and quantitative, into the function of these neurons.

Results and Discussion

One way to accomplish this is via designer receptors exclusively activated by designer drugs (DREADD) technology, in which muscarinic GPCRs are mutated so that their ability to bind natural ligands is lost, while nanomolar potency is gained for the otherwise pharmacologically inert ligand clozapine-N-oxide (CNO) (10, 11). The stimulatory DREADD, designated “hM3Dq”, couples through the Gq pathway to depolarize neurons (10, 11). Here, we used a Cre-recombinase–dependent adeno-associated virus (AAV) to target hM3Dq to AgRP neurons (Figure (Figure1A).1A). The DREADD was fused to mCherry so that receptor expression could be monitored. Stable transgene inversion was achieved using the FLEX Switch (12). When the AAV-hM3Dq-mCherry was stereotaxically injected into the ARC of AgRP-Ires-cre mice, mCherry was detected exclusively in the ARC, in a pattern consistent with AgRP neurons (Figure (Figure1B).1B). Importantly, no expression was detected following injection into non–Cre-expressing mice (data not shown). Furthermore, mCherry was restricted to AgRP–Cre-expressing cells, as revealed by colocalization with GFP in mice also harboring a Cre-dependent GFP reporter transgene (Figure (Figure1C)1C) (>95% of GFP-expressing neurons express mCherry). Notably, GFP protein is cytoplasmic, while the DREADD receptor is expressed on the plasma membrane. Whole cell, current clamp recordings were then performed in mCherry-expressing AgRP neurons. When added to the brain slice, CNO depolarized and markedly increased the firing rate of AgRP neurons (Figure (Figure1D).1D). The effect of CNO-induced depolarization was reversible following washout (Figure (Figure1D).1D). Importantly, CNO did not affect the membrane potential of non–DREADD-expressing AgRP neurons (data not shown). When injected in vivo, CNO greatly increased c-fos immunoreactivity in the ARC in a pattern consistent with AgRP-expressing neurons (Figure (Figure1E),1E), further evidence that this approach acutely and specifically stimulates neuronal activity.

Figure 1
Cre-dependent AAV-hM3Dq-mCherry is specifically expressed in the ARC of AgRP-Ires-cre mice and confers activation by CNO.

One critical advantage of this approach, as it applies to investigating in vivo behavior, is that the same animal can be used as its own control (e.g., saline versus CNO). As an additional control, we also injected wild-type littermate animals with AAV-hM3Dq-mCherry virus into the ARC. Thus, all mice shown in Figures Figures22 and and33 had received bilateral injections of AAV-DREADD-mCherry. However, only the AgRP-Ires-cre mice expressed DREADD in AgRP neurons. Stimulation of AgRP neurons via i.p. injection of CNO (0.3 mg/kg) rapidly (in a matter of minutes) induced feeding (see Supplemental Video 1; supplemental material available online with this article; doi: 10.1172/JCI46229DS1). Mice fed ad lib were injected during the light phase, a time in which mice normally refrain from eating. CNO-injected AgRP-Ires-cre mice ate nearly 4-fold more than controls in the first 30 minutes after injection and continued to eat at a higher rate for the duration of the study (Figure (Figure2A).2A). Furthermore, stimulation of AgRP neurons produced a sharp decline in energy expenditure (Figure (Figure2B),2B), which lasted for approximately 8 hours. Notably, this duration of action for a single injection of CNO is consistent with a previous report (11).

Figure 2
Manipulation of AgRP neuron activity alters energy balance.
Figure 3
Stimulating AgRP neurons drives a behavioral program to work for and search for food.

Chronic stimulation of AgRP neurons via twice-daily injections of CNO caused marked weight gain (Figure (Figure2C).2C). AgRP-Ires-cre mice injected with CNO gained 2.5 g following 1 day of treatment and continued to amass weight at a reduced rate until CNO injections were stopped on day 11 (after 5 days of CNO), at which point the AgRP-Ires-cre mice had gained 4.6 g of body weight (Figure (Figure2C),2C), including 2.9 g of fat (Figure (Figure2D).2D). This substantial weight gain was due to increased food intake (Figure (Figure2E)2E) and, most likely, also decreased energy expenditure (Figure (Figure2B).2B). Of interest, the CNO-induced obesity was completely reversed following 5 days of drug withdrawal (Figure (Figure2,2, C and D), and this reversal was associated with hypophagia (Figure (Figure2E).2E). Complete normalization of body weight and adiposity, caused in part by “compensatory” hypophagia, strongly supports the existence of a set point for these parameters (13).

The above studies strongly support the sufficiency of AgRP neurons to promote body weight gain via increases in food intake and decreases in energy expenditure. To further address whether these same neurons are required for feeding behavior, we used an identical viral approach in which we subsequently expressed the Gi/o-coupled inhibitory DREADD, designated “hM4Di”, in AgRP neurons (10, 11, 14). As assessed by whole cell, current clamp recordings, CNO both hyperpolarized and decreased the firing rate of hM4Di-expressing AgRP neurons (Figure (Figure2F).2F). Notably, inhibition of AgRP neurons in vivo at the onset of the dark cycle (when the majority of food intake normally occurs) significantly reduced food intake (Figure (Figure2G). 2G).

It has been suggested that the hypothalamic neurocircuitry responsible for integrating metabolic signals is embedded within a complex network that permits both the adaptation and synchronization of nutrient needs to conditions in the animal’s local environment (15). It is likely that these same neurons invoke neural mechanisms of reward, motivation, and decision making, all of which are integrated to serve the ultimate goal of obtaining and ingesting food. To determine whether these more complex aspects of feeding behavior are engaged by basal hypothalamic neurons, we stimulated AgRP neurons in mice fed ad lib and then assessed their motivation to work (e.g., via a nose poke beam-break setup) for food reinforcements using a progressive ratio operant conditioning paradigm (16). In this assay, an animal’s willingness to work for food is represented by its breakpoint, the highest number of successive nose pokes a mouse will perform, in a progressively increasing paradigm (i.e., 1, 4, 7), to procure a single food pellet. For these operant conditioning studies, all mice were trained in the absence of CNO on a fixed ratio of 1 (FR1: 1 nose poke/1 pellet) for 5 consecutive days to learn to correctly associate a nose poke with the delivery of food reinforcement. The motivation to obtain food reinforcement was then assessed using a progressive ratio of 3 (PR3). When AgRP neurons were activated by CNO, mice reached a dramatically higher breakpoint (Figure (Figure3A),3A), which was similar in magnitude to that induced by the calorically deficient, fasted state (Figure (Figure3A).3A). Thus, even in a calorically replete state, stimulation of AgRP neurons alone can drive intense motivated behavior for food reinforcement.

To further investigate goal-directed behavior aimed at food acquisition, we measured physical activity (17) in AgRP-Ires-cre and wild-type mice fed ad lib and injected with either saline or CNO during the light cycle. In the first trial, food was removed simultaneously with administration of CNO or saline; in the second trial, food was freely available. Stimulating AgRP neurons in the absence of food led to intense, unrelenting activity, a behavioral state that continued unabated for hours (Figure (Figure3B).3B). We interpreted this as food-seeking behavior because the mice were often seen visiting areas of the cage where food was normally located and were also engaging in vigorous digging-type behaviors (see Supplemental Video 2). When the same mice were again given CNO on a different day, this time in the presence of food, this sharp increase in activity was completely absent (Figure (Figure3B),3B), indicating that the marked activity was directed toward the acquisition and consumption of food.

Cre-dependent AAV-DREADDs, in combination with neuron-specific cre mice, provide a technically easy means of rapidly, specifically, and reversibly regulating neuronal activity. This should empower efforts to unravel the circuitry controlling feeding and its related behaviors. Here, we found that AgRP neuron activity is both necessary and sufficient for feeding — inhibition of AgRP neurons at a time when mice normally eat (at the beginning of the dark cycle) restrains feeding, while stimulation of AgRP neurons when mice are nutritionally replete (near the beginning of the light cycle) compels feeding. Furthermore, we demonstrate that these same neurons drive not only a behavioral program to consume food, but to seek it out as well.


An expanded Methods section is provided in Supplemental Methods.


All animal care and experimental procedures were approved by the Beth Israel Deaconess Medical Center Institutional Animal Care and Use Committee. Mice from a mixed background (129/C57BL6) were housed at 22°C–24°C with a 12-hour light/12-hour dark cycle with standard mouse chow and water provided ad libitum. AgRP-Ires-cre mice have been previously described (4).

Generation of AAV-hM3Dq-mCherry and AAV-hM4Di-mCherry vectors.

The hM3Dq and hM4Di coding sequences were cloned into a mCherry vector (18) upstream of the mCherry sequence to generate C-terminal mCherry fusion proteins. The hM3Dq-mCherry and hM4Di-mCherry coding sequences were amplified by PCR, and the amplicons and a cre-inducible AAV vector with a human Synapsin 1 promoter (refs. 19 and 20; a gift from Karl Deisseroth, Howard Hughes Medical Institute, Department of Bioengineering and Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, California, USA.) were digested with NheI and AscI. The digestion products were ligated such that the coding regions for the fusion proteins were in a 3′ to 5′ orientation relative to the promoter. The final vectors were sequence verified and packaged in serotype 8.

Stereotaxic AAV-DREADD-mCherry injections.

Mice were injected as previously described (21). Briefly, 200-nL bilateral injections were made in the ARC of AgRP-Ires-cre and wild-type littermate controls (coordinates, bregma: anterior-posterior, –1.40 mm; dorsal-ventral, –5.80 mm; lateral, +/–0.30 mm). Mice were allowed 1 week to recover and then acclimated to handling for 1 week before the start of any in vivo studies.


Immunohistochemistry was performed as previously described (22). The primary antibodies used in these studies include rabbit anti-dsRed (Clontech; 1:2,500), chicken anti-GFP (Abcam; 1:1000), and rabbit anti–c-fos (Calbiochem; 1:25,000).


The protocols of slice preparation and whole cell recording are previously described (22). 5- to 7-week-old mice injected with AAV 10–14 days previously were used. After acquisition of stable whole cell recordings on AgRP neurons for 2–5 minutes, artificial cerebral spinal fluid solution containing 5–10 μM CNO was perfused.

Food intake studies.

Food intake studies on chow were performed as previously described (4). Food intake was assessed at different time points following injection of either saline or CNO (0.3 mg/kg) on alternating days in the acute studies (data shown is from male mice) or 5 days straight in the chronic studies (data shown is from female mice). Body composition (fat mass) was analyzed as previously described (4) using EchoMRI.

Energy expenditure measurements.

Energy expenditure was assessed by measuring oxygen consumption as previously described (4) using a comprehensive lab animal monitoring system (CLAMS; Columbus Instruments). Data reported here are from male mice.

Nose poke assay.

Operant conditioning sessions were conducted as previously described (16). Briefly, for 5 consecutive days mice fed ad libitum were injected i.p. with saline, placed in chambers, and acclimated to nose poking for food pellets for a period of 60 minutes under an FR1 operant paradigm.

Following completion of FR1 training, mice fed ad libitum were then exposed to a PR3 testing paradigm (60-minute duration) for the following 6 days and were treated with saline or CNO (0.3 mg/kg) on alternate days. Data are presented as the breakpoint during the PR3. Following the completion of drug treatments, mice were fasted for 24 hours and then tested again under PR3 conditions immediately following injection with saline. Data reported herein are from male mice.

Beam-break assay.

Locomotor activity was assessed as previously described (17). Briefly, individually housed mice were placed in their home cages, and ambulatory counts along the x axis were recorded for 5 hours during the light cycle either in the absence or presence of food. Data reported herein are from male mice.


Statistical analyses were performed using KaleidaGraph (Synergy Software). Overall ANOVA analyses were followed by planned pairwise comparisons between the relevant groups with a Tukey’s honestly significant difference post-hoc test. A P value of less than 0.05 was considered significant in these studies.

Supplementary Material

Supplemental data:
Supplemental video 1:
Supplemental video 2:


This work was supported by NIH grants (R01DK071051, R01DK075632, R01DK089044, and R37DK053477 to B.B. Lowell; R01DK069983 to E. Maratos-Flier; 1F32DK089710-01 to M.J. Krashes; U19MH82441, R01DA027120, R01MH61887, R01DA017204, RAID, and NIMH-PDSP NS064882 to B.L. Roth; and MH087074 and GM008719 to S.C. Rogan). Additionally, B.L. Roth received support from the Michael Hooker Distinguished Chair in Pharmacology, and S.C. Rogan received support from an Elion Award from the Triangle Community Foundation.


Conflict of interest: The authors have declared that no conflict of interest exists.

Citation for this article: J Clin Invest. 2011;121(4):1424–1428. doi:10.1172/JCI46229.


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