Search tips
Search criteria 


Logo of jbacterPermissionsJournals.ASM.orgJournalJB ArticleJournal InfoAuthorsReviewers
J Bacteriol. 2011 April; 193(7): 1672–1680.
Published online 2011 January 28. doi:  10.1128/JB.01487-10
PMCID: PMC3067641

Genome Analyses of Icelandic Strains of Sulfolobus islandicus, Model Organisms for Genetic and Virus-Host Interaction Studies[down-pointing small open triangle]


The genomes of two Sulfolobus islandicus strains obtained from Icelandic solfataras were sequenced and analyzed. Strain REY15A is a host for a versatile genetic toolbox. It exhibits a genome of minimal size, is stable genetically, and is easy to grow and manipulate. Strain HVE10/4 shows a broad host range for exceptional crenarchaeal viruses and conjugative plasmids and was selected for studying their life cycles and host interactions. The genomes of strains REY15A and HVE10/4 are 2.5 and 2.7 Mb, respectively, and each genome carries a variable region of 0.5 to 0.7 Mb where major differences in gene content and gene order occur. These include gene clusters involved in specific metabolic pathways, multiple copies of VapBC antitoxin-toxin gene pairs, and in strain HVE10/4, a 50-kb region rich in glycosyl transferase genes. The variable region also contains most of the insertion sequence (IS) elements and high proportions of the orphan orfB elements and SMN1 miniature inverted-repeat transposable elements (MITEs), as well as the clustered regular interspaced short palindromic repeat (CRISPR)-based immune systems, which are complex and diverse in both strains, consistent with them having been mobilized both intra- and intercellularly. In contrast, the remainder of the genomes are highly conserved in their protein and RNA gene syntenies, closely resembling those of other S. islandicus and Sulfolobus solfataricus strains, and they exhibit only minor remnants of a few genetic elements, mainly conjugative plasmids, which have integrated at a few tRNA genes lacking introns. This provides a possible rationale for the presence of the introns.

Iceland has been a rich source of hyperthermophilic crenarchaea over the past 3 decades and especially of acidothermophilic members of the order Sulfolobales. Many Sulfolobus islandicus strains (“Island” is German for “Iceland”) have also yielded many novel viruses showing varied and sometimes unique morphologies and exceptional genome contents. These properties are consistent with these viruses constituting an archaeal lineage distinct from those of bacteria and eukarya, and they have now been classified into several new viral families (38, 63). In addition, a family of conjugative plasmids has been characterized, with most members deriving from Iceland, which appear to conjugate by a mechanism unique to the archaeal domain (18, 37).

Although the availability of genome sequences of Sulfolobus strains and their genetic elements has yielded important insights into the biology of these model crenarchaea, a major impediment to more detailed insights has been the paucity of robust and versatile vector-host systems for genetic studies. A few Sulfolobus species have been successfully employed as hosts for such systems, including Sulfolobus solfataricus strains P1 and 98/2 (22, 58), Sulfolobus acidocaldarius (57), and S. islandicus strain REY15A (54). To date, the genetic tools developed for the latter host are the most versatile and include the following: (i) Sulfolobus-Escherichia coli shuttle vectors carrying either viral or plasmid replication origins (50); (ii) conventional and novel gene knockout methodologies (14, 62), and (iii) a d-arabinose-inducible expression system with a lacS reporter gene system (35). The S. islandicus system has also been employed successfully to demonstrate the dynamic character of the clustered regular interspaced short palindromic repeat (CRISPR)-based immune systems of Sulfolobus when challenged with genetic elements carrying matching viral gene and protospacers maintained under selection (20). These developments necessitated the determination of the genome sequence of S. islandicus strain REY15A as a prerequisite for successful exploitation of the genetic systems.

A second Icelandic strain, S. islandicus strain HVE10/4, has been employed as a broad laboratory host for propagating diverse Sulfolobus viruses and conjugative plasmids (63) and was selected for in-depth studies of their life cycles and host interactions. This effort received added impetus with the demonstration that some genetic elements show exceptional and sometimes unique properties of their viral life cycles or conjugative mechanisms (3, 8, 18, 40). Therefore, the genome sequence of S. islandicus strain HVE10/4 was also determined.

The genome sequences of two Icelandic strains, REY15A and HVE10/4, were analyzed and compared and contrasted with one another and with genomes of other S. solfataricus and S. islandicus strains isolated from different geographical locations, including Naples, Italy; Kamchatka, Russia; Lassen Volcanic National Park; and Yellowstone National Park (44, 53).


Genome sequencing.

S. islandicus strains REY15A and HVE10/4 were colony purified three times and cultured essentially as described earlier (11). Total DNA was extracted from the cells using phenol-chloroform and further purified by CsCl density-gradient centrifugation. For strain REY15A, sequencing of shotgun libraries with a 454 GS FLX sequenator yielded 324,123 reads with 31-fold genome coverage. For strain HVE10/4, DNA was sonicated to yield fragments in the size range of 1.5 to 4.0 kb, and clone libraries were generated in pUC18 using the SmaI site. Sequencing was performed on MegaBace 1000 sequenators to yield approximately 3-fold sequence coverage, and the sequencing data were combined with a sequencing run using a 454 FLX sequenator to yield approximately 10- to 15-fold coverage. The genome sequences were assembled using the phred/phrap/consed package, contigs were linked by combinatorial PCR using primers matching to each contig end, and the PCR products were sequenced to close the gaps. Remaining ambiguous sequence regions in the genome were identified and resolved by generating and sequencing PCR products. Both genomes were annotated automatically and refined manually.

Sequence analyses.

Open reading frames (ORFs) were predicted with Glimmer (13). Frameshifts were detected and checked by sequencing after manual annotation, and the remaining frameshifts were considered to be authentic. Functional assignments of ORFs are based on searches against GenBank ( and the Conserved Domain Database (CDD) ( tRNA genes were located with tRNAscan-SE (26). Potential noncoding RNAs were predicted by comparison with the untranslated RNAs characterized for S. solfataricus and S. acidocaldarius, in terms of sequence similarity and gene context (see Results). Putative insertion sequence (IS) elements were identified by BLASTN search against the IS Finder database ( All annotations were manually curated using Artemis software (47).


Genome general properties.

Genomes of the two Icelandic strains were sequenced using a combination of sequencing strategies. S. islandicus REY15A was determined primarily by 454 sequencing, while strain HVE10/4 was obtained by a combination of Sanger and 454 sequencing at approximately 30-fold and 10-fold coverage, respectively. Protein-coding genes were annotated in Artemis (47), where start codons for single genes and first genes of Sulfolobus operons were generally located 25 to 30 bp downstream from the archaeal hexameric TATA-like box and only genes within operons were preceded by Shine-Dalgarno motifs, of which GGUG predominates (56). Where alternative start codons were juxtapositioned, we selected the most probable on the basis of its position relative to the putative promoter and/or Shine-Dalgarno motifs or experimental data from closely related organisms.

Dot plots of the two genomes demonstrate long sections of gene synteny. One region of about 0.5 to 0.7 Mb exhibits extensive gene shuffling, and there is a smaller region with a 200-kb inversion bordered by shuffled genes (Fig. (Fig.11 A). Some of the minor irregularities in the dot plot were attributable to insertion or integration events. The synteny is maintained, to a large degree, when each genome is compared to that of S. solfataricus P2, despite the occurrence of a large inversion in the latter, and this is illustrated in a dot plot for the genomes of strain REY15A and S. solfataricus P2 (Fig. (Fig.1B).1B). This extensive gene synteny is surprising, given the high level of transpositional activity occurring in S. solfataricus (Table (Table1)1) (7, 30, 41). A similar pattern was also observed when other pairs of S. islandicus genomes from different geographical locations were compared (48), consistent with a high level of conservation of gene synteny for all the S. solfataricus and S. islandicus genomes.

FIG. 1.
(A) Dot plot of the two Icelandic genomes showing the approximate levels of sequence synteny. The large variable regions extend from about 0.35 to 1.0 Mb. Transposase genes are denoted by black lines along the axes. Putative origins of replication adjacent ...
Summary of genetic properties obtained from genomes of two Icelandic S. islandicus strains and other available S. solfataricus and S. islandicus strains

A phylogenetic tree derived from the available genomes clusters together S. islandicus strains from different geographical locations (44), with S. solfataricus strains P2 and 98/2 being more distantly related (Fig. (Fig.22 and Table Table1).1). The nucleotide sequence identity for the concatenated core genes of the two S. islandicus genomes (Fig. (Fig.1A)1A) is 99.6%, and between all the S. islandicus genomes, it is about 99%. The relatively long branches for individual strains (Fig. (Fig.2)2) arise mainly from differences in gene content of the large variable regions (Fig. (Fig.1A).1A). The degree of sequence identity between the concatenated core genes of the S. islandicus and S. solfataricus genomes is about 90% (Fig. (Fig.22).

FIG. 2.
Neighbor-joining tree based on a gene content matrix, including the conserved, core, and unique genes for each available S. islandicus and S. solfataricus genome (Table (Table1).1). The branch lengths represent the number of differences between ...

Three origins of chromosome replication, demonstrated experimentally for S. solfataricus and S. acidocaldarius (27, 46), are well conserved with respect to both the DNA sequence and flanking gene organization in both of the genomes, albeit with the origin oriC2 being inverted relative to the genomes of S. solfataricus P2 and S. islandicus strain YN1551 (Fig. (Fig.1B).1B). Origin oriC1 lies immediately upstream of cdc6-1, oriC2 is close to cdc6-3, while oriC3 is positioned downstream of the whiP gene (Fig. (Fig.1A).1A). The two cdc6 genes and the whiP gene encode putative replication initiators (45).

Large variable region.

The genomes carry two types of variable regions. The large region, constituting 20 to 25% of each genome, extends approximately from positions encompassing 0.3 to 0.8 Mb and 0.3 to 1.0 Mb for strains REY15A and HVE10/4, respectively (Fig. (Fig.1A).1A). The other class is represented mainly by regions downstream from tRNA genes, where integration events have occurred (Table (Table1;1; also, see below). The large variable region contains about 60% of the potentially transposable IS elements and most of the nonautonomous mobile elements, as well as many degenerate copies of the former (Fig. (Fig.1A).1A). It carries some gene clusters, which are present in one or more of the Sulfolobus genomes, including operons and gene cassettes associated with metabolic pathways, and it contains the diverse CRISPR/Cas and Cmr modules (Table (Table1;1; also, see below). It generally lacks essential genes; for example, no tRNA genes or replication origins are present, and thus, it appears to constitute a region where nonessential genes are collected, interchanged, and exchanged intercellularly and where genetic innovation occurs.

Integration sites.

tRNA gene integration events in Sulfolobus genomes predominantly involve conjugative plasmids and fuselloviruses, and these were also the genetic elements most commonly isolated from acidic hot springs in Iceland (63). Most integration events occur via an archaea-specific mechanism, whereby a viral/plasmid integrase gene recombines into a host tRNA gene and partitions (32). The capture of a genetic element in a chromosome leaves a trace because the intN fragment overlapping the tRNA gene is generally maintained, even if the remainder of the genetic element degenerates or is deleted (51, 52) (Table (Table22 ).

Integration events at tRNA genes showing the sizes and origins of the residual integrated genes

For strains REY15A and HVE10/4, remnants of integrated elements adjoin eight and five tRNA genes, respectively (Table (Table2).2). Most of the integrated genes derive from conjugative plasmids, and fuselloviral genes were detected only at tRNAThr[GGT] in each strain, with an integrated region of unknown origin at tRNAMet[CAT] in strain REY15A. All of the integrated elements are highly degenerate, with IS elements or miniature inverted-repeat transposable elements (MITEs) inserted downstream from the tRNA genes (Table (Table2).2). Given the possibility of multiple integrations of genetic elements occurring at a given tRNA gene, it is difficult to analyze unambiguously the origins of residual integrated genes (42).

In contrast to the two Icelandic strains, the other S. solfataricus and S. islandicus genomes carry intact genetic elements bordered by intN and intC fragments that are all potentially excisable (44, 52). They each show evidence of 2 to 7 tRNA gene integration events, in which the most conserved sites are tRNAPro[GGG] and tRNAAla[GGC], with less common events at tRNALeu[GAG] and different alleles of tRNAArg (Table (Table2).2). For the integrated tRNA genes of the Icelandic strains, there was no significant correlation between the identity of the tRNA anticodon and the frequency of codon usage or between the encoded amino acid and the average number of amino acids in the genome-encoded proteins.

Anti-integration role for tRNA introns.

Each genome carries 45 tRNA genes and 2 to 3 pseudo-tRNA genes all located in conserved regions. Sixteen of the tRNA genes contain introns immediately 3′ to the anticodon, varying in size from 12 to 65 bp, and in contrast to many archaeal tRNA genes, none were detected at other sites (29), although putatively degenerate introns, lacking the capacity to form splicing sites, occur in D-loop regions of tRNAGlu[CTC] and tRNAGlu[TTC]. Moreover, the tRNA genes and introns are highly conserved in sequence between the two genomes, and also with the other six S. islandicus genomes, with very few base changes occurring between the introns of a given tRNA. This high level of tRNA and intron sequence conservation extends to S. solfataricus P2, with only very minor differences observed for about one-third of the genes, and it reinforces the concept that the RNA introns are functionally important (5).

A possible function for the tRNA introns, suggested by the above-described analyses, is that they provide protection against integration of genetic elements into tRNA genes. Integration can be disadvantageous in that pre-tRNA transcription can be impaired. Only two intron-carrying tRNA genes showed evidence of integration events (Table (Table2).2). For the tRNAMet[CAT] gene copies, an intact integrase gene is located downstream from the tRNA gene, while for the tRNAPro[GGG], an overlapping intN fragment is present, but the overlapping sequence does not extend to the intron, suggesting that the intron entered after the integration event. This is consistent with the latter integration event being the most conserved, and probably the most ancient, among Sulfolobus species.

IS elements and the versatile orfB element.

Each genome carries a limited range of IS element types, with some in multiple copies (Table (Table3).3). The IS elements are clustered in the variable genomic region and also downstream from tRNA genes that have undergone integration events (Fig. (Fig.1A).1A). Many of these elements appear to be intact, carrying the inverted terminal repeats (ITRs) required for transposition, but exhibit fragmented transposase genes, which are unlikely to be restored by programmed translational frameshifting, as was observed for some bacterial transposases of the IS1 and IS3 families (28). Although some of these elements may be mobilizable by transposases acting in trans, for over one-third of the IS families present, there is no encoded transposase (Table (Table3).3). Potentially, the most active elements are ISC1200 and ISC1234 in both genomes and ISC1229 in strain HVE10/4 (Table (Table3).3). The two Icelandic S. islandicus strains, together with those from Kamchatka, Russia, carry the lowest number of IS elements (Table (Table1),1), many of which are inactive.

Properties of the IS elements, transposases, and MITEs in the Icelandic genomesa

orfB elements of family IS605, together with elements of the IS6 family (Table (Table3),3), are considered to represent the few classes of transposable elements that are ancestral to the archaeal domain (16). orfB occurs alone, or together with a transposase gene, orfA, in the IS200/605 family of transposable elements. They lack ITRs, and both element types occur commonly in viruses and conjugative plasmids of the Sulfolobales (18, 40) (Table (Table3).3). Exceptionally, strain REY15A and HVE10/4 genomes carry 11 and 16 nearly identical copies of the single orfB elements in unconserved genomic positions, respectively. This is consistent with these being the most active transposable elements in each genome (Table (Table3),3), although it remains uncertain whether they are autonomous or require an OrfA in trans for mobility (16). In addition, the orfB elements are exceptionally adaptable, because a further 8 and 2 copies are physically coupled to copies of ISC1200 for strains REY15A and HVE10/4, respectively (Table (Table3),3), and are potentially cotransposable.

Sulfolobus MITEs.

Only two MITE types were detected in multiple copies in each genome, SMN1 (320 bp) and SM3A (164 bp) (Table (Table3),3), and both of which are capable of nonautonomous transposition in different S. islandicus strains, facilitated by transposases of ISC1733 and ISC1058, respectively (2, 4, 43). All SMN1 copies are located immediately downstream from the sequence TTTAA, but none occur at conserved positions within the two genomes. Clearly, the SMN1 MITEs are active in both of the genomes, as is ISC1733, which encodes the mobilizing transposase (Table (Table3),3), and they appear to be cleanly excised when mobilized, in agreement with the results of an earlier induced excision in the S. islandicus strain REN1H1 (2). Although most SMN1 copies lie in intergenic regions, and may or may not affect regulatory signals, some appear to inactivate or alter genes. Thus, in strain REY15A, an AAA+ ATPase (SiRe0883) and a hypothetical gene (SiRe0925) have incurred insertions in their promoters, and in strain HVE10/4, SMN1 copies partially overlap with two genes (SiH0773/2472), generating altered ORF sequences.

In contrast, the two SM3A copies are conserved in position in each genome, consistent with the mobilizing transposase encoded in ISC1058 being degenerate in both genomes. Nevertheless, each SM3A copy retains the conserved 8-bp inverted terminal repeat of the ISC1058 element (and unconserved 9-bp direct repeats resulting from the transposition event) and can potentially be mobilized if a transposase-encoding ISC1058 element enters the cell. Their maintenance as intact elements may result from one SM3A copy overlapping with the start of a conserved C/D box RNA gene (3), which may alter its transcriptional properties, while the other lies between promoters of two conserved protein genes and may influence their relative transcriptional levels. SM3A occurs in a few copies in each of the sequenced S. islandicus genomes, whereas SMN1 is limited to the Icelandic and three Kamchatka strains, where it occurs in 1 to 5 copies (Table (Table11).

Strain-specific metabolic pathways.

Each Icelandic strain shows a few specific metabolic properties. Thus, the REY15/A strain carries an operon (SiRe0441-0445) encoding enzymes implicated in nitrate reduction and nitrite extrusion, suggesting that it can use nitrate as a terminal electron acceptor for anaerobic respiration. The operon is located in the variable region and has been observed previously only for two other archaea, S. islandicus strains M.14.25 and M.16.27. The larger genome of strain HVE10/4 exclusively carries a urease operon (SiH0978-0983) predicted to encode enzymes involved in the hydrolysis of urea to NH4 and CO2 and previously found only in the archaea Sulfolobus tokodaii, Metallosphaera sedula, and Cenarchaeum symbiosum. Moreover, uniquely for a Sulfolobus species, strain HVE10/4 also carries several genes predicted to encode hydrogenases and hydrogenase maturation enzymes (SiH0883-0892) in the variable region, which suggests that the strain may be able to grow anaerobically.

A 50-kb region of strain HVE10/4 in the variable region (SiH0447-0489) is bordered by IS elements and carries 15 predicted glycosyl transferase genes (group 1 and family 2), constituting about half of the genome copies, interspersed almost exclusively with genes of unknown function and a gene encoding a predicted polysaccharide biosynthesis enzyme. It is well established that Sulfolobus S-layer proteins SlaA and SlaB (SiRe1612/1 and SiH1691/0, respectively) are heavily glycosylated (36), but the relatively low G+C content of the region suggests that it has been inserted and has an alternative unknown function. The genome region is absent from strain REY15A and from some of the other S. islandicus strains (Table (Table11).


Sulfolobus strains utilize different sugars and carbohydrates as carbon and energy sources (19), consistent with their coding capacity for solute ABC transporters. A total of 15 different ABC transporters were identified, of which strain REY15A carries 12 and strain HVE10/4 contains 14. Of these, 11 ABC transporters are present in S. solfataricus P2 (53), 6 in S. tokodaii (23), but only 3 in S. acidocaldarius (9). The other S. islandicus genomes each carry 10 to 14 ABC transporters (44) (Table (Table1).1). In both of the Icelandic genomes, many ABC transporter genes are located in the variable region (Fig. (Fig.1A)1A) and are often flanked by transposons, consistent with their being subjected to loss or gain events.

The ABC transporters are diverse, and some of their solute specificities have been identified for other Sulfolobus strains (15, 24). Cellobiose, maltose, and arabinose transporters are present in both of the Icelandic genomes and most other sequenced S. solfataricus and S. islandicus genomes, although a few S. islandicus strains lack one of the systems, as follows: the arabinose system is absent from strain YG5714, while the maltose system is not present in strains YN1551 and LD215. Strikingly, the transporter of glucose, the preferred carbon source for many microbes, is present only in the Icelandic strains, S. islandicus strains M1415 and YG5714, and in S. solfataricus P2. The lack of specific ABC transporters suggests either that glucose is an uncommon nutrient in hot environments or that another ABC transporter can facilitate glucose transport. One ABC transporter encoded in the variable region of strain HVE10/4 (SiH0899-0903), flanked by IS elements, appears to be unique in public sequence databases.

Toxin-antitoxin systems.

Four of the eight families of antitoxin-toxin complexes characterized for free-living bacteria also occur in archaea, of which the VapBC family is by far the most abundant (34) and is the main antitoxin-toxin family that we detected in the Sulfolobus strains. The Icelandic strains REY15A and HVE10/4 carry 17 and 18 vapBC gene pairs, respectively (Table (Table1),1), as well as 2 vapC-like gene copies coupled to other genes. They are distributed throughout the genomes, with several located in the variable region, and only five gene pairs are conserved in sequence and gene contexts in both strains (SiRe0698/SiH0636, SiRe2073/SiH2137, SiRe2171/SiH2227, SiRe2294/SiH2344, and SiRe2626/SiH2689). Sequence alignments and tree-building exercises demonstrated that the sequences of both antitoxins and toxins within each genome are very diverse and can be classified into subtypes (data not shown), consistent with their functional diversity and targeting of different cellular sites. These data also indicate, for given gene pairs, that the subtypes of VapB and VapC do not always correspond, implying that some gene pairs may have exchanged partners.

Reading frame shifts and mRNA intron splicing.

Examples of translational reading frame shifts yielding single polypeptides have been demonstrated experimentally for S. solfataricus P2 (10). For two of these, a predicted transketolase (SiRe1696/8 and SiH1776/8) and a putative O-sialoglycoprotein endopeptidase (SiRe1569/70 and SiH1648/9), the S. islandicus genes overlap in a similar way and are likely to undergo reading frame shifts. In contrast to S. solfataricus P2, α-fucosidase (SiRe2185 and SiH2241) is a single gene, as is the predicted dihydrolipoamide acyltransferase gene (SiH0582), located only in strain HVE10/4. Very few transposase genes present in IS elements (Table (Table3)3) carry a single reading frame shift that could be expressed as a single protein via translational reading frame shifts (28).

Transcripts of the intron-carrying cbf5 genes (SiRe1607/8 and SiH1686/7) have been demonstrated to be spliced by the archaeal splicing enzyme at the mRNA level in some crenarchaea (60). Other mRNAs, including those encoding the XPD helicase (SiRe1685/SiH1765), have been predicted to undergo splicing, but experimental support is lacking (5).

Noncoding RNAs.

Many untranslated RNAs have been characterized for S. solfataricus and S. acidocaldarius using a variety of techniques, including probing cell extracts for RNA with K-turn binding motifs and generating cDNA libraries of total cellular RNA extracts, as well as numerous antisense RNAs (33, 55, 59, 61). Most of these RNAs were characterized for nucleotide length and partial sequence, and several were detected by more than one experimental approach. We have reanalyzed all these different RNA entities and have annotated the S. islandicus RNA homologs which are conserved in both sequence and gene contexts. The total number of RNA genes and their putative functions are given (Table (Table44).

Summary of Sulfolobus conserved noncoding RNA genes located in the two Icelandic genomes

As for other archaeal hyperthermophiles, each genome carries many C/D box RNAs that methylate primarily rRNAs and tRNAs (Table (Table4).4). In strains REY15A and HVE10/4, 18 and 16 C/D box RNAs target rRNAs, respectively, while 4 modify tRNAs and a further 3 have unknown targets. Two copies of H/ACA RNA genes are present in each genome which, together with the aPus7 protein (SiRe1836 and SiH1908), generate pseudouridine-35 in pre-tRNATyr transcripts (31). Each of these C/D box and H/ACA box RNA genes can be detected in the other available S. islandicus genomes, which underlines their functional importance. Of these, only three RNA genes characterized for other Sulfolobus strains, Sso-sR4, Sso-sR8, and Sso-92, were not located in any S. islandicus genomes (33, 55). For the numerous noncoding RNAs of unknown function, similar contents were found for the two Icelandic strains (Table (Table4)4) and for the other S. islandicus strains, with only a few variations (Table (Table1),1), thereby underlining their functional importance.

Diversity of the CRISPR-based immune systems.

The CRISPR/Cas and Cmr modules all lie within the large variable regions. They show marked heterogeneity in the number and family (25, 48) and are unconserved in position between the genomes (Fig. (Fig.1A).1A). Whereas REY15A carries one paired CRISPR/Cas module of the family I type and two family B Cmr modules, HVE10/4 contains two paired CRISPR/Cas modules of family I and III types and a single family B Cmr module (48) (Fig. (Fig.33 A and B). This diversity of CRISPR-based systems also extends to the other S. solfataricus and S. islandicus genomes (Table (Table1).1). Although the gene content and organization of the paired family I CRISPR/Cas modules are quite conserved among crenarchaea (48), exceptionally, for strain HVE10/4, the internal group of cas genes located between the two leader regions is inverted (Fig. (Fig.3B),3B), indicative of a rearrangement having occurred within the module, possibly via the identical inverted repeat sequences of the bordering leader regions (Fig. (Fig.3B3B).

FIG. 3.
(A) Phylogenetic tree of Cmr2 and its homologues in all sequenced archaeal genomes generates 5 families, A to E. The two Icelandic strains carry family B Cmr modules, for which the gene order is shown. Other sequenced S. islandicus and S. solfataricus ...

The CRISPR loci of strain REY15A carry 115 and 93 spacer-repeat units centered at position 733,000, while those of HVE10/4 contain 116 and 101 repeat-spacer units and 35 and 14 repeat-spacer units centered at positions 364000 and 745000, respectively (Fig. (Fig.1A).1A). No spacer sequence identity was detected within, or between, the two Icelandic strains or with the other S. solfataricus and S. islandicus genomes. None of the available fully sequenced S. islandicus genomes (Table (Table1)1) have any spacers in common, in contrast to the S. solfataricus strains P1, P2, and 98/2, which all share many identical spacers (17, 25) despite their being as distant from one another, phylogenetically, as the S. islandicus strains (Fig. (Fig.2).2). Thus, it seems that diversification of genomic CRISPR loci can occur either by simple spacer turnover or by horizontal transfer of whole or partial CRISPR/cas cassettes. There is increasing evidence for the latter mechanism being the most common one in S. islandicus strains (17, 21).

Since many of the characterized viruses and plasmids of Sulfolobus derive from Iceland, we analyzed the degree to which CRISPR spacer sequences of the Icelandic strains yielded significant matches to genetic element sequences using an earlier approach examining nucleotide and translated sequences of the spacers (25, 49). Several significant sequence matches were detected for both of the genomes, primarily to rudiviruses, fuselloviruses, and conjugative plasmids, all of which are abundant in Icelandic hot springs (63), but also were detected in smaller numbers to other viruses and cryptic plasmids (Fig. (Fig.3B3B).


The genome analyses underline the potential importance of S. islandicus strain REY15A as a model organism for molecular genetic studies of the Sulfolobales, and crenarchaea in general, for a variety of reasons. The genome size of 2.5 Mb is minimal for a Sulfolobus species; moreover, the incidence of mobile elements is relatively low (Table (Table1),1), and stable deletion mutants can be readily isolated (14, 20). Furthermore, the high incidence of diverse ABC transporter systems (Table (Table1)1) may explain why S. islandicus (and S. solfataricus) is most commonly isolated from enrichment cultures obtained from terrestrial acidic hot springs, which is in contrast to, for example, S. acidocaldarius, which carries only three ABC transporters (9, 44, 63).

The relatively high incidence of deletion mutants obtained from strain REY15A occurs despite the presence of several transposable elements. However, in both of the Icelandic strains, many of the IS elements are degenerate or carry disrupted transposase genes (Table (Table3),3), consistent with the “copy-and-paste” transpositional mechanism of most classes of Sulfolobus IS elements and their undetectably low reversibility rate (4, 41). The inability to remove the elements by spontaneous deletion, which does occur in many bacteria (16), may also explain the presence of antisense RNAs in Sulfolobus species to regulate transposase activity (55). The Icelandic strains do, however, carry many copies of orphan orfB elements and SMN1 MITEs, which are mobilized by a “cut-and-paste” mechanism presumably through OrfA encoded in IS element ISC1733 (2, 16). The SMN1 MITEs appear to be specific to the Icelandic and Kamchatka strains (Table (Table1),1), and they can generate genetic novelty, reversibly, by extending open reading frames, in contrast to the other Sulfolobus MITEs, which carry many potential stop codons in all reading frames (43). The absence of most of the known Sulfolobus MITEs, except SM3A, probably reflects the much lower diversity of the mobilizing transposases present (Table (Table3).3). Many of these elements are located in the large variable region where genetic diversification occurs, including the uptake and loss of operons and gene cassettes and rearrangements of mainly nonessential genes. A similar variable genetic region in many genetic elements of Sulfolobus has also been observed (e.g., see reference 18).

Many questions concerning the exceptional molecular and cellular properties of crenarchaeal organisms remain to be resolved. They include the functions of the multiple and highly diverse gene pairs encoding VapBC antitoxin-toxins. For hyperthermophilic Sulfolobus species, in particular, their presence and variety could be a prerequisite for adaptation to life under extreme, and sometimes rapidly varying, temperature and pH conditions, as well as to survival in nutrient-poor environments possibly by optimizing the quality control of gene expression (12, 34). They may also be related to the sulfolobicins implicated in killing competitor Sulfolobus cells (39). The crystal structure of a VapC toxin from the crenarchaeal hyperthermophile Pyrobaculum aerophilum implicated the protein in exonuclease activity (1), but the multiplicity and wide sequence diversity of the vapBC genes suggest that the toxins target different cellular or molecular sites.

Strain HVE10/4 has been used as a host for a variety of genetic elements, mainly from Iceland, which were likely to be genetically close to the Icelandic host (63). The genome analyses provide few insights into why it is a good host, especially since it appears to carry a type 1 restriction-modification system (SiH1435 to SiH1437). Moreover, the CRISPR/Cas and CRISPR/Cmr modules of strain HVE10/4 are relatively complex, as they also are for strain REY15A and other Sulfolobus strains. Their activities have also been demonstrated, at least for strain REY15A, by challenging the CRISPR/Cas systems with vector-borne matching protospacers maintained under selection, which produced deletions of the matching spacers (20). The puzzle remains as to why the Sulfolobus CRISPR-based systems are so complex, given that many of the viruses and plasmids coexist at low copy numbers and are nonlytic. One possibility is that the CRISPR/Cmr system primarily has a regulatory role, with antisense crRNAs (CRISPR RNAs) targeting viral mRNAs. Whatever the reason, the genetic closeness of strains REY15A and HVE10/4 suggests that the former may also be a broad host for viruses and plasmids, with the added advantage that genetic manipulation systems are now available, and our preliminary studies with fuselloviruses and conjugative plasmids support this supposition.


This research was supported by grants from the National Natural Science Foundation of China (grants 306210165, 30730003, and 30870058) to L.H., a grant from the Danish Research Council for Technology and Production (grant 09-062932) to Q.S., and grants from the Danish Natural Science Research Council (grant 272-08-0391) and Danish National Research Foundation to R.A.G.


[down-pointing small open triangle]Published ahead of print on 28 January 2011.


1. Arcus, V. L., K. Bäckbro, A. Roost, E. L. Daniel, and E. N. Baker. 2004. Distant structural homology leads to the functional characterisation of an archaeal PIN domain as an exonuclease. J. Biol. Chem. 279:16471-16478. [PubMed]
2. Berkner, S., and G. Lipps. 2007. An active nonautonomous mobile element in Sulfolobus islandicus REN1H1. J. Bacteriol. 189:2145-2149. [PMC free article] [PubMed]
3. Bize, A., et al. 2009. A unique virus release mechanism in archaea. Proc. Natl. Acad. Sci. U. S. A. 106:11306-11311. [PubMed]
4. Blount, Z. D., and D. W. Grogan. 2005. New insertion sequences of Sulfolobus: functional properties and implications for genome evolution in hyperthermophilic archaea. Mol. Microbiol. 55:312-325. [PubMed]
5. Brügger, K., X. Peng, and R. A. Garrett. 2007. Sulfolobus genomes: mechanisms of rearrangement and charge, p. 95-104. In R. A. Garrett and H.-P. Klenk (ed.), Archaea: evolution, physiology, and molecular biology. Blackwell Publishing, Oxford, United Kingdom.
6. Brügger, K., et al. 2002. Mobile elements in archaeal genomes. FEMS Microbiol. Lett. 206:131-141. [PubMed]
7. Brügger, K., E. Torarinsson, P. Redder, L. Chen, and R. A. Garrett. 2004. Shuffling of Sulfolobus genomes by autonomous and non-autonomous mobile elements. Biochem. Soc. Trans. 32:179-183. [PubMed]
8. Brumfield, S. K., et al. 2009. Particle assembly and ultrastructural features associated with the replication of the lytic archaeal virus Sulfolobus turreted icosahedral virus. J. Virol. 83:5964-5970. [PMC free article] [PubMed]
9. Chen, L., et al. 2005. The genome of Sulfolobus acidocaldarius, a model organism of the Crenarchaeota. J. Bacteriol. 187:4992-4999. [PMC free article] [PubMed]
10. Cobucci-Ponzano, B., et al. 2010. Functional characterisation and high-throughput proteomic analysis of interrupted genes in the archaeon Sulfolobus solfataricus. J. Proteome Res. 9:2496-2507. [PubMed]
11. Contursi, P., et al. 2006. Characterisation of the Sulfolobus host-SSV2 virus interaction. Extremophiles 10:615-627. [PubMed]
12. Cooper, C. R., A. J. Daugherty, S. Tachdjian, P. H. Blum, and R. M. Kelly. 2009. Role of vapBC toxin-antitoxin loci in the thermal stress response of Sulfolobus solfataricus. Biochem. Soc. Trans. 37:123-126. [PMC free article] [PubMed]
13. Delcher, A. L., K. A. Bratke, E. C. Powers, and S. L. Salzberg. 2007. Identifying bacterial genes and endosymbiont DNA with Glimmer. Bioinformatics 23:673-679. [PMC free article] [PubMed]
14. Deng, L., H. Zhu, Z. Chen, Y. X. Liang, and Q. She. 2009. Unmarked gene deletion and host-vector system for the hyperthermophilic crenarchaeon Sulfolobus islandicus. Extremophiles 13:735-746. [PubMed]
15. Elferink, M. G., S. V. Albers, W. N. Konings, and A. J. Driessen. 2001. Sugar transport in Sulfolobus solfataricus is mediated by two families of binding protein-dependent ABC transporters. Mol. Microbiol. 39:1494-1503. [PubMed]
16. Filée, J., P. Siguier, and M. Chandler. 2007. Insertion sequence diversity in archaea. Microbiol. Mol. Biol. Rev. 71:121-157. [PMC free article] [PubMed]
17. Garrett, R. A., et al. 2011. CRISPR-based immune systems of the Sulfolobales: complexity and diversity. Biochem. Soc. Trans. 39:51-57. [PubMed]
18. Greve, B., S. Jensen, K. Brügger, W. Zillig, and R. A. Garrett. 2004. Genomic comparison of archaeal conjugative plasmids from Sulfolobus. Archaea 1:231-239. [PMC free article] [PubMed]
19. Grogan, D. W. 1989. Phenotypic characterization of the archaebacterial genus Sulfolobus: comparison of five wild-type strains. J. Bacteriol. 171:6710-6719. [PMC free article] [PubMed]
20. Gudbergsdottir, S., et al. 2011. Dynamic properties of the Sulfolobus CRISPR/Cas and CRISPR/Cmr systems when challenged with vector-borne viral and plasmid genes and protospacers. Mol. Microbiol. 79:35-49. [PMC free article] [PubMed]
21. Held, N. L., A. Herrera, H. Cadillo-Quiroz, and R. J. Whitaker. 2010. CRISPR associated diversity within a population of Sulfolobus islandicus. PLoS One 5:e12988. [PMC free article] [PubMed]
22. Jonuscheit, M., E. Martusewitsch, K. M. Stedman, and C. Schleper. 2003. A reporter gene system for the hyperthermophilic archaeon Sulfolobus solfataricus based on a selectable and integrative shuttle vector. Mol. Microbiol. 48:1241-1252. [PubMed]
23. Kawarabayasi, Y., et al. 2001. Complete genome sequence of an aerobic thermoacidophilic crenarchaeon, Sulfolobus tokodaii strain 7. DNA Res. 8:123-140. [PubMed]
24. Koning, S. M., S. V. Albers, W. N. Konings, and A. J. Driessen. 2002. Sugar transport in (hyper)thermophilic archaea. Res. Microbiol. 153:61-67. [PubMed]
25. Lillestøl, R. K., et al. 2009. CRISPR families of the crenarchaeal genus Sulfolobus: bidirectional transcription and dynamic properties. Mol. Microbiol. 72:259-272. [PubMed]
26. Lowe, T. M., and S. R. Eddy. 1997. tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. Nucleic Acids Res. 25:955-964. [PMC free article] [PubMed]
27. Lundgren, M., A. Andersson, L. Chen, P. Nilsson, and R. Bernander. 2004. Three replication origins in Sulfolobus species: synchronous initiation of chromosome replication and asynchronous termination. Proc. Natl. Acad. Sci. U. S. A. 101:7046-7051. [PubMed]
28. Mahillon, J., and M. Chandler. 1998. Insertion sequences. Microbiol. Mol. Biol. Rev. 62:725-774. [PMC free article] [PubMed]
29. Marck, C., and H. Grosjean. 2003. Identification of BHB splicing motifs in iintron-containing tRNAs from 18 archaea: evolutionary implications. RNA 9:1516-1531. [PubMed]
30. Martusewitsch, E., C. W. Sensen, and C. Schleper. 2000. High spontaneous mutation rate in the hyperthermophilic archaeon Sulfolobus solfataricus is mediated by transposable elements. J. Bacteriol. 182:2574-2581. [PMC free article] [PubMed]
31. Muller, S., et al. 2009. Deficiency of the tRNATyr:Ψ35-synthase aPus7 in Archaea of the Sulfolobales order might be rescued by the H/ACA sRNA-guided machinery. Nucleic Acids Res. 37:1308-1322. [PMC free article] [PubMed]
32. Muskhelishvili, G., P. Palm, and W. Zillig. 1993. SSV1-encoded site-specific recombination system in Sulfolobus shibatae. Mol. Gen. Genet. 273:334-342. [PubMed]
33. Omer, A. D., M. Zago, A. Chang, and P. P. Dennis. 2006. Probing the structure and function of an archaeal C/D-box methylation guide sRNA. RNA 12:1708-1720. [PubMed]
34. Pandey, D. P., and K. Gerdes. 2005. Toxin-antitoxin loci are highly abundant in free-living but lost from host-associated prokaryotes. Nucleic Acids Res. 33:966-976. [PMC free article] [PubMed]
35. Peng, N., Q. Xia, Z. Chen, Y. X. Liang, and Q. She. 2009. An upstream activation element exerting differential transcription activation on an archaeal promoter. Mol. Microbiol. 74:928-939. [PubMed]
36. Peyfoon, E., et al. 2010. The S-layer glycoprotein of the crenarchaeote Sulfolobus acidocaldarius is glycosylated at multiple sites with chitobiose-linked N-glycans. Archaea pii:754101. [PMC free article] [PubMed]
37. Prangishvili, D., et al. 1998. Conjugation in archaea: frequent occurrence of conjugative plasmids in Sulfolobus. Plasmid 40:190-202. [PubMed]
38. Prangishvili, D., P. P. Forterre, and R. A. Garrett. 2006. Viruses of the Archaea: a unifying view. Nat. Rev. Microbiol. 4:837-848. [PubMed]
39. Prangishvili, D., et al. 2000. Sulfolobicins, specific proteinaceous toxins produced by strains of the extremely thermophilic archaeal genus Sulfolobus. J. Bacteriol. 182:2985-2988. [PMC free article] [PubMed]
40. Prangishvili, D., et al. 2006. Structural and genomic properties of the hyperthermophilic archaeal virus ATV with an extracellular stage of the reproductive cycle. J. Mol. Biol. 359:1203-1216. [PubMed]
41. Redder, P., and R. A. Garrett. 2006. Mutations and rearrangements in the genome of Sulfolobus solfataricus P2. J. Bacteriol. 188:4198-4206. [PMC free article] [PubMed]
42. Redder, P., et al. 2009. Four newly isolated fuselloviruses from extreme geothermal environments reveal unusual morphologies and a possible interviral recombination mechanism. Environ. Microbiol. 11:2849-2862. [PubMed]
43. Redder, P., Q. She, and R. A. Garrett. 2001. Non-autonomous elements in the crenarchaeon Sulfolobus solfataricus. J. Mol. Biol. 306:1-6. [PubMed]
44. Reno, M. L., N. L. Held, C. J. Fields, P. V. Burke, and R. J. Whitaker. 2009. Sulfolobus islandicus pan-genome. Proc. Natl. Acad. Sci. U. S. A. 106:8605-8610. (Erratum, 106:18873.) [PubMed]
45. Robinson, N. P., and S. D. Bell. 2007. Extrachromosomal element capture and the evolution of multiple replication origins in archaeal chromosomes. Proc. Natl. Acad. Sci. U. S. A. 104:5806-5811. [PubMed]
46. Robinson, N. P., et al. 2004. Identification of two origins of replication in the single chromosome of the archaeon Sulfolobus solfataricus. Cell 116:25-38. [PubMed]
47. Rutherford, K., et al. 2000. Artemis: sequence visualization and annotation. Bioinformatics 16:944-945. [PubMed]
48. Shah, S. A., and R. A. Garrett. 2011. CRISPR/Cas and Cmr modules, mobility and evolution of adaptive immune systems. Res. Microbiol. 162:27-38. [PubMed]
49. Shah, S. A., N. R. Hansen, and R. A. Garrett. 2009. Distributions of CRISPR spacer matches in viruses and plasmids of crenarchaeal acidothermophiles and implications for their inhibitory mechanism. Trans. Biochem. Soc. 37:23-28. [PubMed]
50. She, Q., et al. 2008. Host-vector systems for hyperthermophilic archaeon Sulfolobus, p. 151-156. In S.-J. Liu and H. L. Drake (ed.), Microbes and the environment: perspective and challenges. Science Press, Beijing, China.
51. She, Q., X. Peng, W. Zillig, and R. A. Garrett. 2001. Gene capture in archaeal chromosomes. Nature 409:478. [PubMed]
52. She, Q., B. Shen, and L. Chen. 2004. Archaeal integrases and mechanisms of gene capture. Biochem. Soc. Trans. 22:222-226. [PubMed]
53. She, Q., et al. 2001. The complete genome of the crenarchaeon Sulfolobus solfataricus P2. Proc. Natl. Acad. Sci. U. S. A. 98:7835-7840. [PubMed]
54. She, Q., et al. 2009. Genetic analyses in the hyperthermophilic archaeon Sulfolobus islandicus. Biochem. Soc. Trans. 37:92-96. [PubMed]
55. Tang, T.-H., et al. 2005. Identification of novel non-coding RNAs as potential antisense regulators in the archaeon Sulfolobus solfataricus. Mol. Microbiol. 55:469-481. [PubMed]
56. Torarinsson, E., H.-P. Klenk, and R. A. Garrett. 2005. Divergent transcriptional and translational signals in Archaea. Environ. Microbiol. 7:47-54. [PubMed]
57. Wagner, M., et al. 2009. Expanding and understanding the genetic toolbox of the hyperthermophilic genus Sulfolobus. Biochem. Soc. Trans. 37:97-101. [PubMed]
58. Worthington, P., V. Hoang, F. Perez-Pomares, and P. Blum. 2003. Targeted disruption of the alpha-amylase gene in the hyperthermophilic archaeon Sulfolobus solfataricus. J. Bacteriol. 185:482-488. [PMC free article] [PubMed]
59. Wurtzel, O., et al. 2010. A single-base resolution map of an archaeal transcriptome. Genome Res. 20:133-141. [PubMed]
60. Yokobori, S., et al. 2009. Gain and loss of an intron in a protein-coding gene in Archaea: the case of an archaeal RNA pseudouridine synthase gene. BMC Evol. Biol. 9:198. [PMC free article] [PubMed]
61. Zago, M. A., P. P. Dennis, and A. D. Omer. 2005. The expanding world of small RNAs in the hyperthermophilic archaeon Sulfolobus solfataricus. Mol. Microbiol. 55:1812-1828. [PubMed]
62. Zhang, C., et al. 2010. Revealing the essentiality of multiple archaeal pcna genes using a mutant propagation assay based on an improved knockout method. Microbiology 156:3386-3397. [PubMed]
63. Zillig, W., et al. 1998. Genetic elements in the extremely thermophilic archaeon Sulfolobus. Extremophiles 2:131-140. [PubMed]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)