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Nucleic Acids Res. 1987 November 11; 15(21): 8957–8962.
PMCID: PMC306415

Linkage of TNF genes to the HLA-B locus.


Pulsed field gel electrophoresis was used to determine the location of the tumour necrosis factor (TNF) alpha and beta genes. They were shown to be linked to the HLA-B locus; analogous to their location in mouse, between the complement (class III) region and H-2D. However, the distance between the TNF genes and the class I region was much greater in man, namely about 260 kb, compared to 70 kb in the mouse. This finding may have implications for some HLA associated diseases.

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Selected References

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  • Old LJ. Tumor necrosis factor (TNF). Science. 1985 Nov 8;230(4726):630–632. [PubMed]
  • Beutler B, Cerami A. Cachectin and tumour necrosis factor as two sides of the same biological coin. Nature. 1986 Apr 17;320(6063):584–588. [PubMed]
  • Carswell EA, Old LJ, Kassel RL, Green S, Fiore N, Williamson B. An endotoxin-induced serum factor that causes necrosis of tumors. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3666–3670. [PubMed]
  • Aggarwal BB, Kohr WJ, Hass PE, Moffat B, Spencer SA, Henzel WJ, Bringman TS, Nedwin GE, Goeddel DV, Harkins RN. Human tumor necrosis factor. Production, purification, and characterization. J Biol Chem. 1985 Feb 25;260(4):2345–2354. [PubMed]
  • Nedospasov SA, Hirt B, Shakhov AN, Dobrynin VN, Kawashima E, Accolla RS, Jongeneel CV. The genes for tumor necrosis factor (TNF-alpha) and lymphotoxin (TNF-beta) are tandemly arranged on chromosome 17 of the mouse. Nucleic Acids Res. 1986 Oct 10;14(19):7713–7725. [PMC free article] [PubMed]
  • Nedwin GE, Naylor SL, Sakaguchi AY, Smith D, Jarrett-Nedwin J, Pennica D, Goeddel DV, Gray PW. Human lymphotoxin and tumor necrosis factor genes: structure, homology and chromosomal localization. Nucleic Acids Res. 1985 Sep 11;13(17):6361–6373. [PMC free article] [PubMed]
  • Spies T, Morton CC, Nedospasov SA, Fiers W, Pious D, Strominger JL. Genes for the tumor necrosis factors alpha and beta are linked to the human major histocompatibility complex. Proc Natl Acad Sci U S A. 1986 Nov;83(22):8699–8702. [PubMed]
  • Nedospasov SA, Shakhov AN, Turetskaia RL, Mett VA, Georgiev GP. Molekuliarnoe klonirovanie genov cheloveka, kodiruiushchikh faktory nekroza opukholei: tandemnoe raspolozhenie al'fa- i beta-genov v korotkom segmente (6 tys. par nukleotidov) genoma cheloveka. Dokl Akad Nauk SSSR. 1985;285(6):1487–1490. [PubMed]
  • Müller U, Jongeneel CV, Nedospasov SA, Lindahl KF, Steinmetz M. Tumour necrosis factor and lymphotoxin genes map close to H-2D in the mouse major histocompatibility complex. Nature. 1987 Jan 15;325(6101):265–267. [PubMed]
  • Müller U, Stephan D, Philippsen P, Steinmetz M. Orientation and molecular map position of the complement genes in the mouse MHC. EMBO J. 1987 Feb;6(2):369–373. [PubMed]
  • Inoko H, Ando A, Kimura M, Tsuji K. Isolation and characterization of the cDNA clone and genomic clones of a new HLA class II antigen heavy chain, DO alpha. J Immunol. 1985 Sep;135(3):2156–2159. [PubMed]
  • Benton WD, Davis RW. Screening lambdagt recombinant clones by hybridization to single plaques in situ. Science. 1977 Apr 8;196(4286):180–182. [PubMed]
  • Shirai T, Yamaguchi H, Ito H, Todd CW, Wallace RB. Cloning and expression in Escherichia coli of the gene for human tumour necrosis factor. Nature. 313(6005):803–806. [PubMed]
  • Bernards A, Kooter JM, Michels PA, Moberts RM, Borst P. Pulsed field gradient electrophoresis of DNA digested in agarose allows the sizing of the large duplication unit of a surface antigen gene in trypanosomes. Gene. 1986;42(3):313–322. [PubMed]
  • Feinberg AP, Vogelstein B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983 Jul 1;132(1):6–13. [PubMed]
  • Sood AK, Pereira D, Weissman SM. Isolation and partial nucleotide sequence of a cDNA clone for human histocompatibility antigen HLA-B by use of an oligodeoxynucleotide primer. Proc Natl Acad Sci U S A. 1981 Jan;78(1):616–620. [PubMed]
  • Koller BH, Sidwell B, DeMars R, Orr HT. Isolation of HLA locus-specific DNA probes from the 3'-untranslated region. Proc Natl Acad Sci U S A. 1984 Aug;81(16):5175–5178. [PubMed]
  • Strachan T, Dodge AB, Smillie D, Dyer PA, Sodoyer R, Jordan BR, Harris R. An HLA-C-specific DNA probe. Immunogenetics. 1986;23(2):115–120. [PubMed]
  • Schwartz DC, Cantor CR. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell. 1984 May;37(1):67–75. [PubMed]
  • Carle GF, Olson MV. Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis. Nucleic Acids Res. 1984 Jul 25;12(14):5647–5664. [PMC free article] [PubMed]
  • Geczy AF, Alexander K, Bashir HV, Edmonds JP, Upfold L, Sullivan J. HLA-B27, Klebsiella and ankylosing spondylitis: biological and chemical studies. Immunol Rev. 1983;70:23–50. [PubMed]
  • Ozawa A, Ohkido M, Tsuji K. Some recent advances in HLA and skin diseases. J Am Acad Dermatol. 1981 Feb;4(2):205–230. [PubMed]

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