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In atopic individuals, food ingestion drives the production of IgE antibodies that can trigger hypersensitivity reactions. The IL-4 pathway plays critical roles in this response and genetic polymorphisms in its components have been linked to allergy.
To test whether an activating mutation in the IL-4 receptor (IL-4R) α chain enhances allergic responses to a food antigen.
F709 mice, in which the IL-4Rα immuno-tyrosine inhibitory motif (ITIM) motif is inactivated, were gavage fed with ovalbumin (OVA). Reactions to OVA challenge and immune responses including antibody production and Th2 responses were assessed.
F709 mice, but not wild-type (WT) controls, sensitized by gavage with OVA and either cholera toxin (CT) or Staphylococcal enterotoxin B (SEB), displayed mast cell activation and systemic anaphylaxis upon enteral challenge. Anaphylaxis was elicited even in F709 mice enterally sensitized with OVA alone. Bone marrow chimera experiments established that the increased sensitivity conferred by the F709 genotype was mediated mostly by hematopoietic cells but that nonhematopoietic cells also contributed. F709 mice exhibited increased intestinal permeability to macromolecules. The F709 genotype conferred increased OVA-specific IgE but not IgG1 responses, local and systemic Th2 responses and intestinal mast cell hyperplasia as compared with WT mice. Anaphylaxis was abrogated in F709 mice lacking IgE or the high affinity receptor for IgE (FcεRI).
Augmented IL-4Rα signaling confers increased intestinal permeability and dramatically enhanced sensitivity to food allergens. Unlike anaphylaxis to injected antigens, which in rodents can be mediated by either IgE or IgG antibodies, the food-induced response in F709 mice is solely IgE-dependent.
Food-mediated allergic reactions are a growing health problem with an estimated prevalence of 6% in children and 3–4% in the adult population1–3. The cytokines IL-4 and IL-13 play important roles in the induction and effector phases of allergic responses. They bind to and signal via two receptors which share the IL-4Rα chain paired with one of two alternative subunits (γc or IL-13Rα1) to form the type I or type II IL-4 receptors respectively (reviewed in 4, 5). IL-4R type I, which exclusively binds IL-4, is present predominantly in hematopoietic cells. The type II receptor is expressed on multiple cell types and recognizes both IL-4 and IL-13. Both play distinct roles in the development of immune responses.6–8
A large assortment of independent human studies, including work from our group, has linked polymorphisms in the IL-4/IL-13 axis with atopy.4, 9–11 We have used knockin mouse models to directly test the effects of such mutations on allergic responses in a homogenous genetic background. Targeted disruption of the I4R site of IL-4Rα in mice amplifies IgE responses and elicits enhanced allergic responses to inhaled allergens. 12 Mice with a mutation of the immunoreceptor tyrosine-based inhibitory motif (ITIM) at position 709 (Y → F) of IL-4Rα also have an allergic phenotype with markedly elevated IgE levels and susceptibility to allergen-induced airway inflammation13. There is no human equivalent for the F709 mutation. However, the mutation is prototypic of a number of human IL-4Rα polymorphisms that promote receptor signaling and are associated with atopy including IL-4Rα I75V and IL-4Rα Q576R4,11. The effects of IL-4Rα mutations on cell activation by IL-4 and IL-13 extend to nonhematopoietic cells, which do not express the γc chain of the Type I receptor, suggesting that the allergic phenotype of animals harboring these mutations derives from enhanced signaling via both the Type I and II receptors.
In the most severely affected patients, food ingestion can provoke systemic anaphylaxis. In animal models, both IL-4 and IL-13 can prime end organs for enhanced anaphylactic responses.14 A number of studies have demonstrated that anaphylaxis in mice parenterally immunized with antigen and adjuvant and then challenged intravenously can arise by both IgE- and IgG-mediated mechanisms.15–18 Food allergen ingestion by mice drives robust IgE responses as demonstrated by Li and colleagues using peanut extracts 19 Similarly, Brandt et al. have shown that repeated enteral administration of OVA, in mice previously primed intraperitoneally with OVA and alum, leads to OVA-IgE production and IgE dependent mast cell activation with increased intestinal permeability and diarrhea 20. The animals in this “allergic diarrhea” model do not exhibit hypothermia following enteral challenge (parenteral antigen injection is required to elicit systemic responses) 20–23. These responses are IgE- and mast cell-dependent. However, assessment of the relative contribution of IgE antibodies to food hypersensitivity in purely enterally-sensitized and enterally challenged animals has been prevented by the fact that ingestion has a tolerizing effect so that it has been generally difficult to induce robust allergic sensitization by intestinal immunization alone.
We hypothesized that activating mutations of IL-4Rα would enhance the susceptibility of mice to gastrointestinal allergic responses. To test this hypothesis we studied the responses of F709 mice enterally exposed to OVA either with or without adjuvants (CT or SEB) over nine weeks and then challenged by gavage. OVA gavage of sensitized F709 mice triggered intense systemic anaphylaxis. Bone marrow chimera experiments identified hematopoietic cell IL-4R function as the major driver of this effect. These animals had elevated serum levels of OVA-specific IgE. Anaphylactic responses could be elicited even in F709 mice sensitized to OVA in the absence of any adjuvant. Unlike active systemic anaphylaxis following immunization by injection, which can be IgG-mediated and elicited in mice lacking IgE or FcεRI, the allergic reactions of F709 mice to ingested OVA were completely IgE-dependent. Allergen exposed F709 mice exhibited a Th2-biased systemic OVA specific response along with increased gut expression of Th2 cytokine transcripts. They had marked intestinal mastocytosis along with elevations in intestinal IL-9 transcripts and increased intestinal permeability to macromolecules. Our findings suggest that amplification of IL-4Rα signals facilitate allergic sensitization to ingested antigens, impair tolerance, support intestinal mast cell expansion and drive IgE-dependent anaphylactic responses.
Wild-type (Y709) BALB/c mice were purchased from Taconic Farms (Germantown, NY). Igh-7−/− (IgE−/−)16 and C.129.Il4raF709/F709 (F709) mice were each bred onto a BALB/c background (ten generations). F709 mice have been deposited at JAX lab (Strain name: C.129X1-Il4ra<tm3.1Tch>/J; Stock Number 012709). F709/IgE−/− mice were generated by crossing F709 mice with IgE−/−. All mice were housed in a specific pathogen-free environment and were 6 to 12 weeks old. All experiments were carried out in accordance with the IACUC policies and procedures of Children’s Hospital.
For sensitization, Y709, F709, IgE−/− and F709/IgE−/− were treated intragastrically (i.g.) with sterile PBS or 5 mg OVA (Sigma) and 20 μg CT in 250 μl sterile PBS (saline) on days 0, 1, 2 and 7, followed by weekly sensitization with OVA and CT for 8 more weeks. In the 9th week, mice were challenged with a large dose (150 mg) of OVA. Responses to OVA alone were studied in mice exposed to 5 mg OVA as described above in the absence of adjuvant.
Anaphylaxis was assessed in challenged mice by measuring changes in body temperature and recording symptom scores. Temperature changes were measured using transponders placed subcutaneously two days prior to challenge (Implantable Programmable Temperature Transponder™) (Biomedic Data Systems, Seaford, DE). Symptom scores were determined according to criteria shown in Table 1 “(see Table E1 in the Online Repository).”
Recipient mice were irradiated 2 doses of 600 rads 3 hrs apart and were infused immediately with 2.5 ×106 bone marrow cells from congenic donor mice. Mice were treated with Bactrim during a 6 week period of reconstitution and were then subjected to OVA sensitization. Thy1 congenic mice were used and flow cytometry was used to confirm the effectiveness of bone marrow reconstitution.
Mice were sensitized i.v. with 4 μg DNP-IgE. 24h later, they were challenged i.v. with DNP-HSA and core body temperature was recorded.
mMCP-1, total IgE, OVA-IgE and OVA-IgG1 ELISAa were performed as previously described.24
Intestinal permeability was determined as previously described 25. Briefly, mice were gavaged with 20 ml/kg of 22 mg/ml fluorescein isothiocyanate conjugated dextran (FITC-dextran, molecular mass 4 kDa; Sigma, St. Louis, MO) and 1 mg/ml horseradish peroxidase (HRP, MW 44 kDa; Sigma). Blood was obtained 4h later and the concentration of fluorescein in plasma determined by spectrophotofluorometry, using serially diluted samples of the marker as standard. HRP captured anti-HRP on a plate was measured using the TMB substrate system.
One million spleen cells were cultured in triplicate with 200 μg/ml OVA or 0.2 μg/ml CD3 and CD28 in 1ml complete RPMI 1640 with 10% fetal calf serum. Supernatants were collected at 24, 48 and 96 hr and levels of IL-4, IL-5, IL-13 and IFN-γ determined by ELISA according to the manufacturer’s instructions (IL-4, IL-5 and IFN-γ from BD Biosciences; IL-13 from R&D Systems).
Intestinal mast cells were enumerated by microscopic examination of sections of paraffin-embedded jejunal tissue. Tissue sections were stained with chloroacetate esterase. Mast cells were counted in 4–5 complete cross-sections of jejunum as specified in the figure labels.
Quantitative real-time PCR was performed as previously described26. Expression of IL-4, IL-5, IL-13, IFN-γ, IL-33, IL-10 and IL-9 was calculated relative to GAPDH transcripts.
We hypothesized that the F709 mutants, which have enhanced IL-4R signaling, would have exaggerated responses to food allergens. We tested this hypothesis by subjecting F709 mice to enteral sensitization with OVA followed by a gavage challenge. Anaphylaxis was detected by noninvasive measurement of body temperature and observation of symptoms (Fig. 1A, B). A dramatic and rapid drop in core body temperature was observed in F709 mice sensitized with OVA and CT. Enteral challenge of wild-type (Y709) BALB/c mice sensitized by this protocol did not elicit significant responses. Similar reactions were elicited in F709 mice sensitized using SEB in place of CT, as recently described by Ganeshan and colleagues27 “(see Figure E1 in the Online Repository).”
OVA-challenged F709 mice exhibited very obvious signs of anaphylaxis including decreased activity and periods of inactivity, with no response to prodding or touching. Some mice also exhibited trembling and convulsions (Fig. 1B). All F709 animals and none of the controls developed profuse diarrhea (Fig. 1C). Y709 mice had only minimal symptoms. mMCP-1, a marker of mast cell degranulation, was increased in OVA-challenged F709 but not Y709 mice suggesting that mast cell activation drove the physiologic responses (Fig. 1D).
Anaphylactic sensitivity to food allergens in humans is strongly correlated with the production of high-titer IgE antibodies. Total IgE levels were markedly elevated in both control and experimental F709 mice as compared to Y709 mice (Fig. 1E). OVA-IgE responses were detected only in antigen-exposed mice with levels of 2,459±2136 ng/ml in Y709 mice and 11,159±2458ng/ml in F709 mice respectively (Fig. 1F). In contrast, and somewhat unexpectedly, anti-OVA IgG1 responses, which are also Th2-dependent, were comparable in Y709 and F709 mice treated with OVA and CT (Fig. 1G).
Both bone marrow-derived and nonhematopoietic cells including epithelial cells express IL-4R. In order to determine the relative contributions of IL-4R function on hematopoietic vs. nonhematopoietic cells to the F709 allergic phenotype, we assessed the responses of BM chimeras. Irradiated Y709 recipients reconstituted with F709 BM developed robust anaphylactic responses, similar to those observed in F709 animals (Fig. 2A). The induction of anaphylaxis was fatal in at least 2 animals and was accompanied by severe symptoms (data not shown). All of the animals exhibited diarrhea (Fig. 2B) and had elevated levels of mMCP-1 (Fig. 2C). In contrast, the development of anaphylaxis in F709 recipients receiving BM from Y709 donors was modest, and only 1 animal had significant decreases in core body temperature (Fig. 2A). Moreover, the presence of diarrhea and the levels of mMCP-1 were also attenuated in these animals. Determination of antibody responses revealed the presence of enhanced anti-OVA IgE and anti-OVA IgG1 responses in F709 → Y709 mice (Fig. 2D & 2E). These observations indicate that the augmentation of anaphylactic responses in the IL-4Rα mutant, F709, is largely mediated by hematopoietic cells with a modest additional contribution from nonhematopoietic cell types.
In rodents immunized by parenteral administration of antigen and adjuvant, systemic anaphylaxis to intravenous antigen challenge can occur in the absence of IgE in a reaction driven by IgG antibodies.17, 28 In order to determine the role of IgE in the anaphylactic responses of F709 mice to food antigens we compared the responses of F709 mice with those of double mutants lacking IgE (F709/IgE−/−). As expected, F709 mice in this experiment again exhibited robust responses to intragastric OVA challenge (Fig. 3A). Surprisingly, the development of anaphylaxis was completely abrogated in the absence of IgE in F709/IgE−/− mice (Fig. 3A). In contrast to F709 mice, these mice displayed no clinical symptoms and did not exhibit any diarrhea (data not shown). Their mMCP-1 levels (Fig. 3B) were only about 1% of those observed in F709/IgE+/+ mice (Fig. 1D) and were not significantly different from saline controls. The dependence of the anaphylactic response on IgE was confirmed in separate experiments, in which F709/FcεRI−/− double mutant mice were sensitized with OVA and SEB “(see Fig. E2 in Online Repository).” Examination of the levels of OVA-specific IgG1 showed that both groups involved exhibited similar IgG1 responses (Fig. 3C). These findings indicate that the presence of a strong IL-4R signal during enteral sensitization has a stronger effect on IgE than on IgG responses and that IgE is required for the expression of systemic anaphylaxis to ingested antigens in animals that have been sensitized enterally.
We hypothesized that enhanced signaling by the IL-4R might enhance allergic sensitization in F709 mice and investigated whether ingestion of OVA alone would provoke responses. Unlike Y709 mice, F709 animals fed OVA alone showed significant responsiveness upon challenge (Fig. 4A). Diarrhea occurred in some of these animals (Fig. 4B) and detectable elevations in mMCP-1 were present in five out of six mice studied (Fig. 4C). OVA-specific IgE responses were also detectable in the majority of animals fed with OVA alone (Fig. 4D). None of the Y709 mice fed OVA alone generated mMCP-1 or IgE responses. In contrast, both Y709 and F709 mice exhibited enhanced OVA-IgG1 levels (Fig. 4E). Such allergic responses to protein antigens ingested in the absence of any adjuvant represents a remarkable and unique feature of the F709 model and strongly indicates that activation of IL-4Rα signaling can dampen normal tolerogenic mechanisms.
Inspection for chloroacetate esterase reactivity in sections of jejunum revealed the presence of numerous mucosal mast cells both in mice treated with OVA and CT and those receiving OVA alone (Fig. 5A). Enumeration of mast cells showed that OVA and CT-treated F709 mice had about twice as many mast cells (41±12cells/5 LPF) as Y709 mice (21±4cells/5 LPF) (Fig. 5B). Similarly, F709 mice receiving OVA alone had 45±15cells/5 LPF vs. 5±5cells/5 LPF for Y709 mice. The F709 animals not only exhibited more mast cells but also had mucosal mast cells high up in the villi, a site which might favor interaction with absorbed food proteins. These results indicate that IL-4Rα signaling supports mast cell differentiation in the gut following food allergen exposure and that the intense anaphylactic responses of F709 mice might, in part, be due to the presence of large numbers of these key effector cells. Mast cell expansion was also observed in F709/IgE−/− mice (Fig. 5D), suggesting that IgE antibodies are not required for the recruitment and expansion of intestinal mast cells as we have previously reported for allergen-induced airway mast cells 26 (see also Fig. E4 in the Online Repository). The jejunal mast cells in F709/IgE−/− mice had a consistently unactivated morphology whereas many of the mast cells in IgE-sufficient mice exhibited features of degranulation. Levels of mMCP-1 were much higher in F709 than in F709/IgE−/− mice (Fig. 3B vs. Fig. 1D) consistent with ongoing IgE-mediated activation.
We hypothesized that F709 mutants would have enhanced local and systemic Th2 responses to ingested OVA. Except for IL-10, analysis of jejunal RNA for levels of IL-4, IL-13, IL-5, IL-9, IL-33 and IFN-γ transcripts in Y709 mice exposed to OVA and CT revealed no statistically significant increases in expression of any of these cytokines. In contrast, the relative levels of IL-4, IL-5, IL-13, IL-9 and IL-10 (Fig. 6A–F) were all significantly increased in F709 mice gavaged with OVA and CT in comparison with respective controls. Similar increases were observed for these cytokines as well as IL-33 in the group treated with OVA alone. (Fig. 6A–G). These results indicate that enhanced signaling via IL-4R is permissive for the induction of a local intestinal Th2 response to food allergens. The intense induction of IL-9, a cytokine which also supports mast cell development and recruitment, suggests a possible mechanism for the mast cell responses to food allergen observed in the F709 mice.
In order to test systemic Th2 responses to food allergens, we measured IL-4, IL-13 and IL-5 in the supernatants of spleen cells from saline and CT, OVA and CT, and OVA alone treated mice stimulated with OVA “(see Fig. E3 in the Online Repository)”. As expected, F709 mice mounted strong systemic Th2 anti-OVA responses with markedly more elevated splenocyte production of IL-4, IL-13 and IL-5 than either saline-sensitized controls or OVA-sensitized Y709 mice. Similarly, the levels of serum IL-4 were also significantly enhanced in these animals. These observations confirm that the local Th2 response to OVA observed in the intestines of F709 mice translates into a strong systemic response.
The abrogation of anaphylaxis in F709/IgE−/− mice as well as the enhanced levels of OVA-IgE in F709 vs. Y709 controls implicate the intensity of IgE responses as the major determinant of anaphylactic sensitivity. However, an alternative explanation for heightened sensitivity might be an increased sensitivity of target tissues expressing the F709 variant of IL-4Rα to mast cell-derived mediators. In order to distinguish these possibilities, we examined the intensity of passive antigen-specific IgE-mediated anaphylactic responses in Y709 and F709 mice. Sensitization with DNP-specific IgE, followed by challenge with antigen elicited robust responses with a similar magnitude in Y709 and F709 animals (Fig. 7A) suggesting that the increased sensitivity to anaphylaxis in animals harboring the disinhibited IL-4Rα variant, F709 is linked to IgE production rather than target tissue sensitivity.
Both the relative ease of sensitization to food allergens delivered enterally and the robust anaphylactic responses exhibited by F709 mice following oral challenge suggested that these animals might have altered intestinal barrier function. This could affect antigen uptake during sensitization as well as exposure of mast cells to antigen upon challenge. Both IL-4 and mast cells have been shown to affect intestinal permeability29–31. To determine whether alterations in IL4R signaling might affect intestinal permeability we gavaged F709 mutants as well as control Y709 mice with two macromolecules FITC-dextran (4 kDa) and HRP (~44 kDa) and followed their appearance in the blood. F709 mice exhibited enhanced absorption of FITC-dextran compared with Y709 animals (Fig. 7B), consistent with increased intestinal uptake, likely via a paracellular path. In contrast, HRP was barely detectable in the plasma of either Y709 or F709 mice (Fig. 7C) suggesting limited permeability for this protein which likely penetrates the intestinal epithelium via both paracellular and transcellular transport mechanisms. As mast cell mediators are known to enhance gut permeability31, we next determined whether uptake of these two tracers might be further altered following mast cell activation by IgE:antigen. We therefore followed levels of FITC-dextran and HRP in the blood of mice whose mast cells had been activated by i.v. infusion of anti-DNP IgE followed by DNP-HSA. Both FITC-dextran and HRP uptake were significantly enhanced in F709 animals but not Y709 mice following mast cell activation (Figs. 7B & 7C), suggesting that enhanced IL-4R signaling renders both paracellular and transcellular pathways of intestinal epithelial antigen uptake more sensitive to mast cell-derived mediators.
The findings reported here indicate that augmented IL-4 receptor signaling is singularly effective in promoting both the development of allergic sensitization to ingested protein antigens and the induction of anaphylactic responses upon enteral challenge. Studies in humans and mice have previously established that IL-4 and IL-13 play central roles in the pathogenesis of gastrointestinal allergic responses.32–38 Genetic polymorphisms affecting the IL-4/IL-13 axis are reported to be correlated with asthma susceptibility4, 9–11 and risk associations between polymorphisms in the IL-4, IL-4Rα and IL-13 genes and food allergy have been reported.39, 40 We therefore hypothesized that augmented IL-4 signaling could predispose to food allergy and tested this in F709 mice in which the sensitivity of IL-4Rα signaling is enhanced resulting in increased IL-4 induced STAT6 phosphorylation, Th2 induction and IgE production13.
Our results demonstrate that the F709 genotype confers a very dramatic sensitivity to food allergen and that this phenotype is mediated primarily by IL-4R signaling effects in hematopoietic cells. Mice with this mutation exhibited not only increased susceptibility to immune sensitization, producing both specific IgE antibodies and Th2 responses, but also a marked intestinal mast cell expansion, enhance intestinal permeability and a capacity to mount intense anaphylactic responses upon enteral challenge. Perhaps the single most notable phenotype of these mice with respect to food allergy was their susceptibility both to immune sensitization and to enteral allergen-induced anaphylaxis even when exposed only to a protein antigen, OVA, in the absence of any immune stimulating adjuvant. Taken together, these observations indicate that IL-4 signaling plays critical roles in food allergy by enhancing allergic sensitization, promoting mast cell expansion and anaphylactic sensitivity and by interfering with the physiologic induction of tolerance to new protein antigens introduced via the gastrointestinal tract.
A number of groups have developed murine models to study food-allergen mediated hypersensitivity reactions in mice.19, 20, 27, 41–47. Studies by Rothenberg and colleagues using an “allergic diarrhea” have established a central role for the IL-4/IL-13 axis in the development of food allergy. Our findings confirm and advance this observation by demonstrating that enhanced IL-4R signaling is permissive for purely enteral immune sensitization in the absence of parenteral priming with adjuvant as is used in the allergic diarrhea protocol. Furthermore, we find that the IL-4R effect also results in the manifestation of vascular responses (hypothermia) upon purely enteral challenge (as opposed to i.v. challenge as in the allergic diarrhea system)..
Recently it was shown that IL-4 can divert developing Treg to a cytokine profile dominated by IL-9.48 IL-9, produced by epithelial and other cells, is an important mast cell growth and differentiation factor and has been implicated in intestinal anaphylaxis in an IgE-dependent manner.21, 49–51 Forbes and colleagues have elegantly demonstrated that IL-9-driven mast cell expansion in the intestine leads to enhanced intestinal permeability21. This finding suggests a mechanism whereby the enhanced IL-4Rα signaling of F709 mice, which is accompanied by high levels of intestinal IL-9 transcripts and markedly enhanced mast cell numbers, might increase the intestinal permeability in these mice. We and others have observed that recruitment of mast cell progenitors to allergen challenged lung in sensitized mice is IL-9 dependent.52–54 Dramatically increased jejunal IL-9 levels were observed in the F709 mutants in our study (Fig 6), suggesting pro-amplifying effects of the intestinal epithelium on mast cell accumulation. This is further borne out by the finding, that although the sensitivity to anaphylaxis is largely mediated by hematopoietic cells such as mast cells, non-hematopoietic cells also contribute.
IgE antibodies, which in combination with mast cells serve as exquisitely sensitive biological sensors for allergen, have long been implicated as the key trigger of anaphylactic reactions in allergic individuals. Osterfeld and colleagues have shown that reactions to food allergens in parenterally sensitized mice are IgE dependent23. Thus, at first glance, our finding that the robust anaphylactic responses expressed by F709 mice in response to food allergen challenge were abrogated in IgE−/−F709 animals might not seem surprising. It is important to note though that anaphylaxis in rodents can be driven by completely IgE-independent mechanisms and that the IgG isotype can substitute for IgE.16, 17, 28 Our data show for the first time that IgE is critical in the development of active anaphylaxis to food antigens using exclusively oral routes for both sensitization as and challenge. This is in contrast to our previous findings which showed that parenterally mediated systemic active anaphylaxis could occur in the absence of IgE 16.
Strait and colleagues have shown that while the IgE pathway is more sensitive, requiring lower threshold levels of antigen for full activation, IgG-mediated responses, occurring at appropriate antibody to antigen ratios can nevertheless be lethal. Our observations regarding anaphylactic responses in response to enteral OVA in F709 mice provide important information regarding the relative contributions of IgE and IgG isotypes in the gut. Although OVA specific IgE responses were markedly higher in F709 mice than in Y709 controls, IgG1 antibodies to OVA were similar in the two groups. This finding indicates a preferential effect of IL-4Rα signaling on IgE vs. IgG production in intestinal immune responses to food allergens. More interesting though, was our finding that, in the absence of IgE, the presence of IgG1 responses in both Y709 and F709 animals was insufficient to drive anaphylactic reactions. Neither IgE−/− mice nor F709/IgE−/− double mutants displayed physiologic signs of anaphylaxis upon enteral allergen challenge despite having significant anti-OVA IgG1 titers. This observation suggests that either the amount of absorbed antigen in the gut is insufficient to trigger non-IgE pathways or that the local effector cells of anaphylaxis, likely the markedly expanded mast cells, are preferentially IgE responsive. This observation certainly lends support to the concept currently being evaluated in clinical trials that anti-IgE therapy might block food allergy responses.
Our bone marrow chimera data suggest a partial role of heightened target organ responsiveness to mast cell mediators in the anaphylaxis sensitivity of F709 mice. Finkelman and colleagues have demonstrated that administration of IL-4 immediately prior to anaphylactic challenge results in markedly stronger physiologic reactions because of enhanced tissue sensitivity to platelet activating factor, histamine, serotonin, and cysteinyl leukotrienes.14, 55 Nonhematopoietic cells express predominantly the Type II IL-4/13 receptor (IL-4Rα and IL-13Rα1).
In addition to providing important information regarding potential effects of amplified IL-4 signaling on immune sensitization, mast cell responses and anaphylaxis, we believe that the F709 mice will provide a powerful tool for investigators studying food allergy. Furthermore, the failure of enteral tolerance in these mice will permit not only investigations of physiologic tolerogenic mechanisms but may additionally provide a practical model for evaluating allergenicity of food proteins in the absence of any immune stimulating adjuvants. We anticipate that the observations presented in this report, along with the increasing availability of mouse strains with alterations in IL-4 receptor function will lead to fruitful future investigations of the pathogenesis of food allergy.
We thank Ms. Diana Kombe, Mr. Benjamin Caplan and Ms. Alanna Darling for expert technical assistance.
This project was supported by National Institutes of Health grants: NIAID R01-AI054471 and 1R21AI087666 (HCO) (HCO), 5T32AI007512-24 (CBM), 2R01AI065617 and 1R21AI080002 (TAC), AI083516 (MFG)
Therapeutic interventions that target the IL-4R pathway may be beneficial in restoring oral tolerance in food-allergic individuals.
Conflict of Interest Statement
None of the authors have a financial relationship with a commercial entity that has an interest in the subject of this manuscript.
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