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Biotechnol Res Int. 2011; 2011: 658493.
Published online Oct 11, 2010. doi:  10.4061/2011/658493
PMCID: PMC3042664
Production and Optimization of Cellulase Enzyme Using Aspergillus niger USM AI 1 and Comparison with Trichoderma reesei via Solid State Fermentation System
C. K. Lee,1* I. Darah,1 and C. O. Ibrahim2
1Industrial Biotechnology Research Laboratory, School of Biological Sciences, Universiti Sains Malaysia, Minden,11800 Penang, Malaysia
2Faculty of Agroindustry and Natural Resource, Universiti Malaysia Kelantan, Karung Berkunci 36, Pengkalan Chepa, 16100 Kelantan, Malaysia
*C. K. Lee: cklee1311/at/
Academic Editor: Manuel Canovas
Received June 8, 2010; Revised August 7, 2010; Accepted September 22, 2010.
Novel design solid state bioreactor, FERMSOSTAT, had been evaluated in cellulase production studies using local isolate Aspergillus niger USM AI 1 grown on sugarcane bagasse and palm kernel cake at 1 : 1 (w/w) ratio. Under optimised SSF conditions of 0.5 kg substrate; 70% (w/w) moisture content; 30°C; aeration at 4 L/h · g fermented substrate for 5 min and mixing at 0.5 rpm for 5 min, about 3.4 U/g of Filter paper activity (FPase) was obtained. At the same time, comparative studies of the enzymes production under the same SSF conditions indicated that FPase produced by A. niger USM AI 1 was about 35.3% higher compared to Trichoderma reesei. This shows that the performance of this newly designed SSF bioreactor is acceptable and potentially used as prototype for larger-scale bioreactor design.
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