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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
Circ Res. Author manuscript; available in PMC 2012 February 18.
Published in final edited form as:
PMCID: PMC3042536
NIHMSID: NIHMS267062

Simultaneous Measurement of Protein Oxidation and S-nitrosylation during Preconditioning and Ischemia-Reperfusion Injury with Resin-Assisted Capture

Mark J Kohr, Ph.D.,1,2 Junhui Sun, Ph.D.,2 Angel Aponte, B.S.,3 Guanghui Wang, Ph.D.,3 Marjan Gucek, Ph.D.,3 Elizabeth Murphy, Ph.D.,2 and Charles Steenbergen, M.D., Ph.D.1

Abstract

Rationale

Redox modifications play an important role in many cellular processes, including cell death. Ischemic preconditioning (IPC) has been shown to involve redox signaling. Protein S-nitrosylation (SNO) is increased following myocardial IPC, and SNO is thought to provide cardioprotection, in part, by reducing cysteine oxidation during ischemia/reperfusion (IR) injury.

Objective

To test the hypothesis that SNO provides cardioprotection, in part, by shielding against cysteine oxidation following IR injury.

Methods and Results

We developed a new method to measure protein oxidation using resin-assisted capture (Ox-RAC), which is similar to the SNO-RAC method used in the quantification of SNO. Langendorff-perfused hearts were subjected to various perfusion protocols (control, IPC, IR, IPC-IR, IPC-R) and homogenized. Each sample was divided into two equal aliquots, and the SNO-RAC/Ox-RAC procedure was performed in order to simultaneously analyze SNO and oxidation. We identified 31 different SNO proteins with IPC, 27 of which showed increased SNO compared to baseline. Of the proteins that showed significantly increased SNO with IPC, 76% showed decreased oxidation or no oxidation following ischemia and early reperfusion (IPC-IR) at the same site when compared to IR alone; for non-SNO proteins, oxidation was reduced by only 50%. We further demonstrated that IPC-induced protein SNO is quickly reversible.

Conclusions

These results support the hypothesis that IPC-induced protein SNO provides cardioprotection by shielding cysteine residues from ROS-induced oxidation during IR injury. Therefore, the level of protein SNO plays a critical role in IR injury, where ROS production is increased.

Keywords: ischemic preconditioning, S-nitrosothiol, reactive oxygen species

Introduction

The interplay between reactive oxygen species (ROS) and nitric oxide (NO), referred to as the nitroso-redox balance, is critical in the regulation of myocardial function. Redox modified protein thiols are known to alter the function of key proteins in the myocardium, including the L-type Ca2+ channel,1 the sarcoplasmic reticulum Ca2+-ATPase,2, 3 and the sarcoplasmic reticulum Ca2+ release channel (RyR2).4, 5 RyR2 appears to be particularly sensitive to redox modification, such that the interplay between protein S-nitrosylation and oxidation appears to be crucial in regulating channel activity. The nitroso-redox balance is also critically important during the setting of ischemia-reperfusion (IR) injury which results in widespread thiol oxidation as a consequence of the burst of ROS that occurs in the first few minutes of reperfusion following ischemia. The end result of this widespread oxidation is a nitroso-redox imbalance. Ischemic preconditioning (IPC), which develops from transient episodes of IR and renders the heart resistant to damage resulting from subsequent sustained periods of ischemia, has been shown to involve redox signaling.6 Furthermore, IPC has been shown to reduce the initial burst of ROS, and oxidative stress is thought to be important in cell death.7

NO has been shown to play a key role in cardioprotection.8 The cGMP-dependent effects of NO in IPC are well documented and occur via activation of the mitochondrial KATP channel.9 cGMP-independent effects of NO also play a significant role in cardioprotection.2, 10, 11 Treatment with the S-nitrosylating agent S-nitrosoglutathione (GSNO) increases protein S-nitrosylation and induces cardioprotection.2 S-nitrosylation is a reversible, redox-dependent protein modification in which a NO moiety is covalently attached to the free thiol of a cysteine residue.12-14 This modification has been demonstrated to modify the activity of target proteins.1, 2, 15 Protein targets which have been shown to be S-nitrosylated include aconitate hydratase, aldehyde dehydrogenase, α-ketoglutarate dehydrogenase, mitochondrial complex I, creatine kinase, F1F0-ATPase, malate dehydrogenase, sarcoplasmic reticulum Ca2+-ATPase, and thioredoxin.2, 11, 16, 17

S-nitrosylation is also proposed to protect cysteine residues against potential oxidative damage from ROS.18-20 Many ROS-induced protein modifications are irreversible, and if they alter protein function and/or denature proteins, this will lead to a period of sustained myocardial dysfunction following IR injury. Since S-nitrosylation is a transient modification, S-nitrosylation of proteins during IPC could shield cysteine residues from irreversible oxidation during the first few minutes of reperfusion, after which time the S-nitrosylation is removed and normal protein function can resume. At this point, the initial burst of ROS is complete and the protein is protected from oxidative damage. Thus, the level of S-nitrosylation could play a critical role in IR injury, when ROS production is increased.21

Therefore, it is of interest to determine if S-nitrosylation can exert cardioprotective effects by reducing cysteine oxidation (Fig. 1a). In order to test this hypothesis, the simultaneous measurement and site identification of both S-nitrosylation and oxidation is required. Forrester et al. recently published a technique for measuring and identifying the sites of protein S-nitrosylation (SNO-RAC).22 Herein we describe a new protocol based on the SNO-RAC method, for the measurement and site determination of protein cysteine oxidation (Ox-RAC). This newly developed protocol, when performed in tandem with the SNO-RAC technique, provides the methods necessary to address the important question of whether IPC-induced protein S-nitrosylation shields critical cysteine residues against oxidation.

Figure 1
Hypothesis, treatment protocols and methodology

Materials and Methods

Animals

Male C57BL/6 mice (12-15 weeks) were obtained from Jackson Laboratories (Bar Harbor, ME). This investigation conforms to the Guide for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH publication No. 85-23, revised 1996) and was approved by the Institutional Laboratory Animal Care and Use Committee.

Perfusion protocols, whole heart homogenate preparation, and enzyme assays

Hearts were Langendorff-perfused as previously described2, 23 and snap frozen in liquid nitrogen immediately following the treatment protocol; treatment protocols are shown in Fig. 1b. Whole heart homogenate preparations2, 23 and GAPDH activity measurements24 were performed as described previously.

Oxidation site identification with resin-assisted capture

For oxidation site identification (Ox-RAC, Fig. 1c), samples (1 mg) were diluted in HEN buffer containing (in mmol/L): HEPES-NaOH 7.7 (250), EDTA (1), and Neocuproine (0.1) with 2.5% SDS and an EDTA-free protease inhibitor tablet (Roche Diagnostics Corporation, Indianapolis, IN). All buffers were de-gassed prior to use in order to prevent oxidation of the resin. Homogenates were then incubated with 20 mmol/L ascorbate (Sigma, St. Louis, MO) for 45 minutes at room temperature in order to remove SNO. The inclusion of this step serves to distinguish SNO from other oxidative modifications; this step can be eliminated for the combined examination of SNO and other oxidative modifications. Samples were then incubated with 50 mmol/L N-ethylmaleimide (NEM; Sigma) for 20 minutes at 50°C in order to block unmodified (i.e., free) and ascorbate-reduced thiol groups from modification; ascorbate and NEM were removed via acetone precipitation. Samples were then resuspended in HEN with 1% SDS (HENS) and oxidized thiols were reduced with 10 mmol/L dithiothreitol (DTT; Pierce, Rockford, IL) for 10 minutes at room temperature; DTT was removed via acetone precipitation. Samples were then resuspended in HENS. Thiopropyl sepharose (GE Healthcare, Piscataway, NJ) was rehydrated for 25 minutes in DEPC H2O. Following rehydration, 25 μL of the resin slurry was added to a Handee Mini Spin Column (Pierce) and washed with 5 × 0.5 mL DEPC H2O, followed by 10 × 0.5 mL HEN buffer. Blocked samples were then added to the thiopropyl sepharose-containing spin column and rotated for 4 hours in the dark at room temperature. Proteins bind to the resin by forming disulfide linkages between reduced thiol groups of the protein and the thiol groups of the resin. Resin-bound proteins were then washed with 8 × 0.5 mL HENS buffer, followed by 4 × 0.5 mL HENS buffer diluted 1:10. Samples were then subjected to trypsin digestion (sequencing grade modified; Promega, Madison, WI) overnight at 37°C with rotation in buffer containing (in mmol/L): NH4HCO3 (50) and EDTA (1). Resin-bound peptides were then washed with 5 × 0.5 mL HENS buffer diluted 1:10, 5 × 0.5 mL 2 mol/L NaCl, 5 × 0.5 mL 80% acetonitrile/0.1% trifluoroacetic acid, and 5 × 0.5 mL HEN buffer diluted 1:10. Peptides were eluted for 30 minutes at room temperature in elution buffer containing (in mmol/L): DTT (20), NH4CO3 (10), and 50% methanol. The resin was then washed with an additional volume of elution buffer, followed by 2 volumes of DEPC water. All fractions were combined and concentrated via speedvac. Samples were then resuspended in 0.1% formic acid, and cleaned with a C18 column (ZipTip; Millipore, Billerica, MA). Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was then performed using an LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific, San Jose, CA). The MASCOT database search engine was used for protein identification. Relative quantification of SNO and oxidation was performed using an in-house software program designed as a label-free approach to peptide quantification by LC-MS/MS.25

S-nitrosylation site identification with resin-assisted capture

A modified version of the SNO-RAC protocol was used to examine protein SNO (Fig. 1c).22 Briefly, samples (1 mg) were diluted in HEN buffer with 2.5% SDS and an EDTA-free protease inhibitor tablet (Roche Diagnostics Corporation). All buffers were de-gassed prior to use in order to prevent oxidation of the resin. Homogenates were then incubated with 50 mmol/L NEM for 20 minutes at 50°C in order to block unmodified (i.e., free) thiol groups from modification; NEM was removed via acetone precipitation. Samples were then resuspended in HENS and subjected to the same procedure as described above for the Ox-RAC protocol, with the exception that samples were not reduced with DTT and were co-incubated with thiopropyl sepharose and 20 mmol/L ascorbate to reduce SNO.

Statistics

Statistical significance (p<0.05) was determined between groups using an ANOVA for multiple groups or a Student's t-test for two groups.

For additional information regarding MATERIALS AND METHODS, please see the Online Supplement available at http://circres.ahajournals.org.

Results

To test whether S-nitrosylation (SNO) provides transient protection of cysteine residues against oxidation, we developed the oxidation-resin-assisted capture (Ox-RAC) protocol for the measurement and site identification of protein oxidation, and used this in tandem with the SNO-RAC method (see Fig. 1c).22 The Ox-RAC protocol uses a combination of procedures adapted from the SNO-RAC method and redox difference electrophoresis (redox DIGE).26 We made several modifications to the original SNO-RAC method so that it would be better suited for use in combination with the Ox-RAC protocol. These changes included the use of N-ethylmaleimide (NEM) rather than methyl methanethiosulfonate (MMTS) as a blocking agent. This substitution was made because MMTS is susceptible to dithiothreitol (DTT) reduction during the step in which peptides are eluted from the resin. Conversely, NEM is not affected by DTT. This is particularly important with peptides that contain multiple cysteine residues; unmodified cysteine residues (i.e., not SNO or oxidized) will remain blocked with NEM and will be detected as such by mass spectrometry, thus allowing us to distinguish unmodified cysteine residues from SNO or oxidized cysteine residues (which will not be labeled with NEM) in the case of peptides that have multiple cysteines. The MMTS would be removed by DTT during the elution step and unmodified cysteine residues would not be distinguishable from SNO or oxidized cysteine residues in peptides with multiple cysteines.

As a negative control for the Ox-RAC protocol, whole heart homogenates were pretreated with ascorbate and blocked with NEM. Non-DTT-reduced samples were then incubated with the resin, subjected to trypsin digestion, and analyzed via LC-MS/MS. Only one cysteine-containing peptide was detected by LC-MS/MS, indicating that ascorbate pre-treatment is effective at reducing S-nitrosylation, and that NEM is effective at blocking both unmodified and ascorbate-reduced thiol groups. These data also demonstrate that DTT-dependent false positive signals with the Ox-RAC protocol are extremely low. Further, the use of a higher concentration of ascorbate (50 mmol/L) yielded similar results to that observed with 20 mmol/L ascorbate. Identifications from non-specifically bound peptides (i.e., non-cysteine containing peptides) accounted for ~6% of all peptide identifications; non-cysteine containing peptides were filtered from the data set. These data demonstrate that the SNO-RAC and Ox-RAC techniques are very specific for the identification of SNO and oxidized cysteine residues, respectively.

Ischemic preconditioning transiently increases protein S-nitrosylation

Using the modified SNO-RAC protocol, we identified a total of 33 unique SNO proteins (47 sites corresponding to 39 peptides; note some proteins have multiple peptides and/or multiple cysteines on the same peptide) among the four perfusion protocols (see Table 1 and Fig. 2a). We identified 11 proteins at baseline, an additional 20 SNO proteins with IPC, and two additional SNO proteins with IPC-IR. All 11 of the SNO proteins observed at baseline were also found with IPC, along with 20 additional proteins for a combination of 31 SNO proteins (45 sites corresponding to 37 peptides). Label-free peptide analysis was performed for the 11 common proteins comparing baseline and IPC, and we determined that seven of eleven proteins showed a significant increase in SNO with IPC compared to baseline. Note that this includes isocitrate dehydrogenase [NADP], which had one peptide detected at baseline that did not show a significant increase and a new peptide with IPC that was significant. These SNO proteins included glyceraldehyde-3-phosphate dehydrogenase (GAPDH), lactate dehydrogenase, and triosephosphate isomerase. The remaining four common proteins did not show an increase in SNO with IPC compared to baseline. After 20 minutes of ischemia and 5 minutes of reperfusion, the total number of SNO proteins identified in IPC-IR samples decreased by ~50% (16 proteins) compared to IPC alone, indicating that IPC-induced SNO formation is quickly reversible. Two of the SNO proteins (2 sites corresponding to 2 peptides) consistently observed with IPC-IR were not observed with IPC alone. Following 20 minutes of ischemia and 5 minutes of reperfusion in the absence of IPC, the total number of SNO proteins identified via SNO-RAC fell to 6 proteins and of these six proteins, five showed a significant decrease in SNO compared to baseline.

Figure 2
SNO-RAC and Ox-RAC protein identifications
Table 1
Peptides showing cysteine S-nitrosylation as identified via SNO-RAC proteomic analysis

In order to determine if the reduction in protein SNO observed with IPC-IR occurred as a result of IR or was a time-dependent process, we conducted an additional set of SNO-RAC experiments. Hearts (n = 5) were subjected to a perfusion protocol identical to that used for IPC-IR, with the exception that hearts were not exposed to an ischemic period (IPC-R). Instead, hearts were subjected to a post-IPC reperfusion period equal to that of the combined duration of the ischemic and reperfusion periods of the IPC-IR protocol (Fig. 1b). Following four cycles of IPC and a 25 minute reperfusion period, the number of SNO proteins detected in IPC-R samples was similar to that observed with IPC-IR (12 proteins vs. 16 proteins). This result indicates that IPC-induced protein SNO is transient and decays with time.

Ischemic preconditioning reduces protein oxidation

Using the newly developed Ox-RAC protocol, we identified a large set of oxidized proteins for each of the four perfusion protocols (Fig. 2b). A subset of these oxidized proteins can be found in Online Table I, which shows oxidation site identifications for the same proteins identified in Table 1. As shown in Online Table II, we identified 158 proteins that showed constitutive oxidative modification at baseline. Although there are a large number of proteins with some degree of oxidation at baseline, this number includes proteins with disulfide bonds and iron-sulfur groups. There were a total of 127 proteins that showed oxidation with all four perfusion protocols. Following IR injury, we found 181 oxidized proteins (Online Table III); more than 35% of the common proteins between perfusion and IR showed a significant increase in protein oxidation. Interestingly, 17 unique proteins were oxidized with IR alone and were not detected in the other perfusion protocols. These 17 unique proteins may include those responsible for the detrimental effects of IR-induced oxidation. With IPC, we detected 177 oxidized proteins (Online Table IV), including 13 unique proteins that were not detected in the other perfusion protocols. A closer examination of the proteins oxidized with IPC compared to those oxidized with IR revealed that the two populations of oxidized proteins were very different and, excluding proteins common among all four perfusion protocols (127 proteins), only 18 proteins were shared (~35% similarity). Following IPC-IR, 162 oxidized proteins were identified (Online Table V). The drop in oxidized proteins with IPC-IR compared to IPC alone may result, in part, from the IPC-induced activation of oxidant repair mechanisms. Additionally, 138 common proteins were identified between IR and IPC-IR, and 55% of these common proteins showed a significant reduction in cysteine oxidation with IPC-IR compared to IR alone. Note that Online Tables II-V provide all protein/peptide identifications, including those detected in fewer than three of five samples.

Ischemic preconditioning-induced protein S-nitrosylation provides a direct protective effect against cysteine oxidation following IR injury

Consistent with the hypothesis that SNO can provide direct protection against cysteine oxidation, we examined the oxidation state of the 27 proteins that showed a significant increase in SNO with IPC (42 sites corresponding to 34 peptides). Of these proteins, six did not show any oxidation at the sites of SNO in either IR or IPC-IR and were excluded from comparison. Of the remaining 21 SNO proteins, 76% (16 of 21 proteins) showed a significant reduction in oxidation or no oxidation at all following IPC-IR at the same site when compared to IR alone (Table 2). For example, we detected oxidation of isocitrate dehydrogenase [NAD] subunit α with IR alone, but we did not detect oxidation of this protein following IPC-IR. In contrast, only 50% of non-SNO proteins showed a significant reduction in oxidation. Taken together, these data suggest that IPC-induced protein SNO can protect from cysteine oxidation, and this occurs in part, through a direct block against oxidation.

Table 2
Oxidation state of peptides showing a significant increase in cysteine S-nitrosylation with IPC as identified via SNO-RAC/Ox-RAC proteomic analysis

IPC-induced S-nitrosylation of GAPDH results in faster recovery of activity during reperfusion

We performed a set of experiments examining GAPDH activity in whole heart homogenates subjected to the perfusion protocols shown in Fig. 1b. GAPDH activity was largely inhibited with IPC and following IR (Fig. 3a), and this is likely due to an increase in SNO (Fig. 3b) and/or oxidation (Fig. 3c). Both SNO and oxidation have been shown to inhibit GAPDH activity.24, 27 Interestingly, GAPDH activity levels returned to baseline with IPC-IR, as did SNO and oxidation levels, thus providing further evidence that IPC-induced protein SNO provides direct and transient protection against cysteine oxidation.

Figure 3
Ischemic preconditioning-induced protein S-nitrosylation provides protection against cysteine oxidation

Discussion

Protein S-nitrosylation is known to increase following IPC,2 and is thought to contribute to cardioprotection, in part, by reducing the oxidation of critical cysteine residues. This hypothesis is illustrated in Fig. 1. It has been suggested that cysteine residues which are S-nitrosylated during cardioprotection are shielded from oxidation at the start of reperfusion.18-20 Further, it is well known that the burst of ROS generated during the first few minutes of reperfusion results in irreversible oxidation of many critical proteins. This hypothesis requires that S-nitrosylation be a transient modification; S-nitrosylation would need to be present on the cysteine residue at the start of reperfusion and during the initial burst of ROS (which is thought to last only a few minutes), but the S-nitrosylation would then need to degrade quickly after the burst of ROS so that normal protein function could resume. A rigorous test of this hypothesis requires a method for the measurement and identification of oxidation sites, as well as S-nitrosylation sites. Here we describe a new method for measuring the oxidation sites of proteins in tandem with the measurement of S-nitrosylation. This Ox-RAC method was adapted from the recently published SNO-RAC method.22 We also modified the SNO-RAC technique to accommodate the simultaneous measurement of oxidation and S-nitrosylation, thus allowing us to directly examine the hypothesis that S-nitrosylation shields cysteine residues from oxidation. Utilizing this novel methodology, we have identified a large set of S-nitrosylated and oxidized proteins in our model of cardioprotection (Fig. 2), and find that 76% of proteins showing an IPC-induced increase in S-nitrosylation, exhibit reduced or no oxidation at the same site following ischemia and early reperfusion (Table 2). Further, the IPC-induced increase in protein S-nitrosylation is quickly reversed during early reperfusion (Fig. 3). These results are consistent with the hypothesis that S-nitrosylation provides protection against cysteine oxidation during IR injury.

Nitroso-redox balance in cardioprotection

IPC has been shown to reduce the burst of ROS that occurs during early reperfusion.7 Consistent with this observation, we find that IPC reduced protein oxidation during early reperfusion (see Fig. 2). Compared to IR alone, which is a condition in which the formation of ROS is favored,21 there was a clear reduction in protein oxidation with IPC-IR. Interestingly, there were 17 proteins that were only oxidized with IR and these proteins might include irreversibly damaged proteins that contribute to dysfunction and cell death. Among the proteins oxidized with IR alone was a subunit of the mitochondrial import inner membrane translocase, Tim8B, and proteasome activator complex subunit 1. The mechanism through which IPC reduces protein oxidation is not well characterized, but is known to include a reduction in reperfusion ROS. NO has been shown to reduce reperfusion ROS, suggesting an important role for NO. Oxidant defense and/or oxidant repair mechanisms may also be activated with IPC. The reduction in reperfusion ROS is likely to be separate from the ability of S-nitrosylation to shield cysteine residues against ROS-induced oxidation. We hypothesize that NO, acting in part via S-nitrosylation, can also modify protein function during ischemia and early reperfusion and therefore play an additional role in protection. We have previously demonstrated that S-nitrosylation of the mitochondrial F1F0-ATPase dose dependently decreased activity.2 This effect is consistent with the reduced ATP consumption observed in cardioprotective mechanisms.28 IPC has also been proposed to signal through redox-sensitive mechanisms and antioxidants have been shown to block IPC-mediated protection.6 Interestingly, IPC resulted in the oxidation of 13 unique proteins, including F-actin-capping protein subunit β, and heat shock protein 60, which is an important mitochondrial chaperone protein. Furthermore, the fact that the proteins oxidized with IPC are quite dissimilar from those oxidized with IR alone and are quickly reversed with IPC-IR, may provide further evidence for redox-sensitive signaling.

Evaluation of the Ox-RAC method

We report on a novel procedure for the measurement and site determination of protein cysteine oxidation. We identified 158 proteins with oxidative (non-S-nitrosylation) modifications at baseline. The majority of these proteins showed oxidative modification under all four conditions (Perfusion, IPC, IR, IPC-IR). These modifications include disulfide bonds, sulfenic acids, glutathiolation, and others. A number of methods have been described to measure redox sensitive cysteine residues.26, 29, 30 Hurd et al. used redox DIGE with a backward labeling strategy and, upon treatment of rat heart mitochondria with hydrogen peroxide, found 40 proteins with increased cysteine oxidation (this included S-nitrosylation as they did not pre-treat with ascorbate).26 The use of more physiological ROS generators (i.e., antimycin treatment, stimulation of reverse electron transport) yielded only 6 oxidized proteins. Sites of oxidation were not identified with this method. Fu et al. utilized ICAT-labeling and DIGE with a forward labeling strategy to examine oxidized cysteine residues in extracts with and without hydrogen peroxide.30 This method identified 63 proteins as targets of hydrogen peroxide; ICAT identified 60 sites from 50 proteins. Thus, the Ox-RAC method compares favorably with published protocols. This technique allows for high-throughput identification of oxidized proteins and modification sites under biologically relevant conditions. As a result, this method promises to provide a new tool for further assessing the role of redox signaling in cardiovascular function.

Study Limitations

For the small percentage of peptides identified with the SNO-RAC protocol that have multiple S-nitrosylation sites, some of these cysteine residues may actually be sites of oxidative modification. This possibility exists because oxidative modifications, if present on resin-bound peptides, could be reduced by DTT during the elution step, thus leaving a free cysteine residue. SNO-RAC/Ox-RAC identifications from non-specifically bound peptides are also possible. However, identifications from non-cysteine containing peptides accounted for approximately 6% of all peptide identifications for both the SNO-RAC and Ox-RAC protocols.

Summary

The nitroso-redox balance is crucial in the maintenance of normal myocardial function and this balance can be critically affected by the widespread oxidation that occurs with IR injury. This newly developed SNO/Ox-RAC protocol provides the means to assess the nitroso-redox balance in the myocardium. When applied to a model of IPC, we determined that S-nitrosylation plays an important cardioprotective role by shielding cysteine residues from ROS-induced oxidation, thus maintaining the nitroso-redox balance.

Supplementary Material

Acknowledgments

Sources of Funding: This work was supported by NIH grants 1F32HL096142 (MK) and 5R01HL039752 (CS), and the NHLBI/NIH Intramural Program (AA, EM, GW, JS, & MG).

Non-Standard Abbreviations and Acronyms

cGMP
Cyclic guanosine monophosphate
DIGE
Difference gel electrophoresis
DTT
Dithiothreitol
GAPDH
Glyceraldehyde-3-phosphate dehydrogenase
GSNO
S-nitrosoglutathione
ICAT
Isotope coded affinity tag
IPC
Ischemic preconditioning
IPC-IR
Ischemic preconditioning-ischemia/reperfusion
IPC-R
Ischemic preconditioning-reperfusion
IR
Ischemia/reperfusion
LC-MS/MS
Liquid chromatography-tandem mass spectrometry
MMTS
Methyl methanethiosulfonate
MS
Mass spectrometry
NEM
N-ethylmaleimide
NO
Nitric oxide
Ox
Oxidation
RAC
Resin-assisted capture
RYR2
Sarcoplasmic reticulum Ca2+ release channel
ROS
Reactive oxygen species
SNO
S-nitrosylation

Footnotes

Disclosures: None.

References

1. Sun J, Picht E, Ginsburg KS, Bers DM, Steenbergen C, Murphy E. Hypercontractile female hearts exhibit increased S-nitrosylation of the L-type Ca2+ channel alpha1 subunit and reduced ischemia/reperfusion injury. Circ Res. 2006;98:403–411. [PubMed]
2. Sun J, Morgan M, Shen RF, Steenbergen C, Murphy E. Preconditioning results in S-nitrosylation of proteins involved in regulation of mitochondrial energetics and calcium transport. Circ Res. 2007;101:1155–1163. [PubMed]
3. Adachi T, Weisbrod RM, Pimentel DR, Ying J, Sharov VS, Schoneich C, Cohen RA. S-Glutathiolation by peroxynitrite activates SERCA during arterial relaxation by nitric oxide. Nat Med. 2004;10:1200–1207. [PubMed]
4. Wang H, Viatchenko-Karpinski S, Sun J, Gyorke I, Benkusky NA, Kohr MJ, Valdivia HH, Murphy E, Gyorke S, Ziolo MT. Regulation of myocyte contraction via neuronal nitric oxide synthase: role of ryanodine receptor S-nitrosylation. J Physiol. 588:2905–2917. [PubMed]
5. Gonzalez DR, Beigi F, Treuer AV, Hare JM. Deficient ryanodine receptor S-nitrosylation increases sarcoplasmic reticulum calcium leak and arrhythmogenesis in cardiomyocytes. Proc Natl Acad Sci U S A. 2007;104:20612–20617. [PubMed]
6. Chen W, Gabel S, Steenbergen C, Murphy E. A redox-based mechanism for cardioprotection induced by ischemic preconditioning in perfused rat heart. Circ Res. 1995;77:424–429. [PubMed]
7. Vanden Hoek T, Becker LB, Shao ZH, Li CQ, Schumacker PT. Preconditioning in cardiomyocytes protects by attenuating oxidant stress at reperfusion. Circ Res. 2000;86:541–548. [PubMed]
8. Jones SP, Bolli R. The ubiquitous role of nitric oxide in cardioprotection. J Mol Cell Cardiol. 2006;40:16–23. [PubMed]
9. Costa AD, Garlid KD, West IC, Lincoln TM, Downey JM, Cohen MV, Critz SD. Protein kinase G transmits the cardioprotective signal from cytosol to mitochondria. Circ Res. 2005;97:329–336. [PubMed]
10. Lima B, Lam GK, Xie L, Diesen DL, Villamizar N, Nienaber J, Messina E, Bowles D, Kontos CD, Hare JM, Stamler JS, Rockman HA. Endogenous S-nitrosothiols protect against myocardial injury. Proc Natl Acad Sci U S A. 2009;106:6297–6302. [PubMed]
11. Burwell LS, Nadtochiy SM, Tompkins AJ, Young S, Brookes PS. Direct evidence for S-nitrosation of mitochondrial complex I. Biochem J. 2006;394:627–634. [PubMed]
12. Handy DE, Loscalzo J. Nitric oxide and posttranslational modification of the vascular proteome: S-nitrosation of reactive thiols. Arterioscler Thromb Vasc Biol. 2006;26:1207–1214. [PubMed]
13. Hess DT, Matsumoto A, Kim SO, Marshall HE, Stamler JS. Protein S-nitrosylation: purview and parameters. Nat Rev Mol Cell Biol. 2005;6:150–166. [PubMed]
14. Martinez-Ruiz A, Lamas S. S-nitrosylation: a potential new paradigm in signal transduction. Cardiovasc Res. 2004;62:43–52. [PubMed]
15. Sun J, Xin C, Eu JP, Stamler JS, Meissner G. Cysteine-3635 is responsible for skeletal muscle ryanodine receptor modulation by NO. Proc Natl Acad Sci U S A. 2001;98:11158–11162. [PubMed]
16. Tao L, Gao E, Bryan NS, Qu Y, Liu HR, Hu A, Christopher TA, Lopez BL, Yodoi J, Koch WJ, Feelisch M, Ma XL. Cardioprotective effects of thioredoxin in myocardial ischemia and reperfusion: role of S-nitrosation. Proc Natl Acad Sci U S A. 2004;101:11471–11476. [PubMed]
17. Chouchani ET, Hurd TR, Nadtochiy SM, Brookes PS, Fearnley IM, Lilley KS, Smith RA, Murphy MP. Identification of S-nitrosated mitochondrial proteins by S-nitrosothiol difference in gel electrophoresis (SNO-DIGE): implications for the regulation of mitochondrial function by reversible S-nitrosation. Biochem J. 2010;430:49–59. [PMC free article] [PubMed]
18. Sun J, Steenbergen C, Murphy E. S-nitrosylation: NO-related redox signaling to protect against oxidative stress. Antioxid Redox Signal. 2006;8:1693–1705. [PMC free article] [PubMed]
19. Zimmet JM, Hare JM. Nitroso-redox interactions in the cardiovascular system. Circulation. 2006;114:1531–1544. [PubMed]
20. Sun J, Murphy E. Protein S-nitrosylation and cardioprotection. Circ Res. 2010;106:285–296. [PMC free article] [PubMed]
21. Zweier JL, Talukder MA. The role of oxidants and free radicals in reperfusion injury. Cardiovasc Res. 2006;70:181–190. [PubMed]
22. Forrester MT, Thompson JW, Foster MW, Nogueira L, Moseley MA, Stamler JS. Proteomic analysis of S-nitrosylation and denitrosylation by resin-assisted capture. Nat Biotechnol. 2009;27:557–559. [PMC free article] [PubMed]
23. Lin J, Steenbergen C, Murphy E, Sun J. Estrogen receptor-beta activation results in S-nitrosylation of proteins involved in cardioprotection. Circulation. 2009;120:245–254. [PMC free article] [PubMed]
24. Padgett CM, Whorton AR. S-nitrosoglutathione reversibly inhibits GAPDH by S-nitrosylation. Am J Physiol. 1995;269:C739–C749. [PubMed]
25. Wang G, Wu WW, Zeng W, Chou CL, Shen RF. Label-free protein quantification using LC-coupled ion trap or FT mass spectrometry: Reproducibility, linearity, and application with complex proteomes. J Proteome Res. 2006;5:1214–1223. [PubMed]
26. Hurd TR, Prime TA, Harbour ME, Lilley KS, Murphy MP. Detection of reactive oxygen species-sensitive thiol proteins by redox difference gel electrophoresis: implications for mitochondrial redox signaling. J Biol Chem. 2007;282:22040–22051. [PubMed]
27. Ishii T, Sunami O, Nakajima H, Nishio H, Takeuchi T, Hata F. Critical role of sulfenic acid formation of thiols in the inactivation of glyceraldehyde-3-phosphate dehydrogenase by nitric oxide. Biochem Pharmacol. 1999;58:133–143. [PubMed]
28. Murry CE, Jennings RB, Reimer KA. Preconditioning with ischemia: a delay of lethal cell injury in ischemic myocardium. Circulation. 1986;74:1124–1136. [PubMed]
29. Wetzelberger K, Baba SP, Thirunavukkarasu M, Ho YS, Maulik N, Barski OA, Conklin DJ, Bhatnagar A. Postischemic deactivation of cardiac aldose reductase: role of glutathione S-transferase P and glutaredoxin in regeneration of reduced thiols from sulfenic acids. J Biol Chem. 285:26135–26148. [PubMed]
30. Fu C, Hu J, Liu T, Ago T, Sadoshima J, Li H. Quantitative analysis of redox-sensitive proteome with DIGE and ICAT. J Proteome Res. 2008;7:3789–3802. [PMC free article] [PubMed]