PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

 
Logo of sigsLink to Publisher's site
 
Stand Genomic Sci. Jul 20, 2009; 1(1): 21–28.
Published online Jul 20, 2009. doi:  10.4056/sigs.1162
PMCID: PMC3035217
Complete genome sequence of Beutenbergia cavernae type strain (HKI 0122T)
Miriam Land,1,2 Rüdiger Pukall,3 Birte Abt,3 Markus Göker,3 Manfred Rohde,4 Tijana Glavina Del Rio,1 Hope Tice,1 Alex Copeland,1 Jan-Fang Cheng,1 Susan Lucas,1 Feng Chen,1 Matt Nolan,1 David Bruce,1,5 Lynne Goodwin,1,5 Sam Pitluck,1 Natalia Ivanova,1 Konstantinos Mavromatis,1 Galina Ovchinnikova,1 Amrita Pati,1 Amy Chen,6 Krishna Palaniappan,6 Loren Hauser,1,2 Yun-Juan Chang,1,2 Cynthia C. Jefferies,1,2 Elizabeth Saunders,5 Thomas Brettin,1,5 John C. Detter,1,5 Cliff Han,1,5 Patrick Chain,1,7 James Bristow,1 Jonathan A. Eisen,1,8 Victor Markowitz,6 Philip Hugenholtz,1 Nikos C. Kyrpides,1 Hans-Peter Klenk,3 and Alla Lapidus1*
1DOE Joint Genome Institute, Walnut Creek, California, USA
2Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA
3DSMZ - German Collection of Microorganisms and Cell Cultures GmbH, Braunschweig, Germany
4HZI - Helmholtz Centre for Infection Research, Braunschweig, Germany
5Los Alamos National Laboratory, Bioscience Division, Los Alamos, New Mexico USA
6Biological Data Management and Technology Center, Lawrence Berkeley National Laboratory, Berkeley, California, USA
7Lawrence Livermore National Laboratory, Livermore, California, USA
8University of California Davis Genome Center, Davis, California, USA
*Corresponding author: Alla Lapidus
Beutenbergia cavernae (Groth et al. 1999) is the type species of the genus and is of phylogenetic interest because of its isolated location in the actinobacterial suborder Micrococcineae. B. cavernae HKI 0122T is a Gram-positive, non-motile, non-spore-forming bacterium isolated from a cave in Guangxi (China). B. cavernae grows best under aerobic conditions and shows a rod-coccus growth cycle. Its cell wall peptidoglycan contains the diagnostic L-lysine ← L-glutamate interpeptide bridge. Here we describe the features of this organism, together with the complete genome sequence, and annotation. This is the first completed genome sequence from the poorly populated micrococcineal family Beutenbergiaceae, and this 4,669,183 bp long single replicon genome with its 4225 protein-coding and 53 RNA genes is part of the Genomic Encyclopedia of Bacteria and Archaea project.
Keywords: mesophile, non-pathogenic, aerobic and microaerophilic, rod-coccus growth cycle, MK-8(H4), actinomycete, Micrococcineae
Beutenbergia cavernae strain HKI 0122T (DSM 12333 = ATCC BAA-8 = JCM 11478) is the type strain of the species, which represents the type species of the genus Beutenbergia, the type genus of the family Beutenbergiaceae [1]. B. cavernae was described by Groth et al. 1999 as Gram-positive, non-motile and non-spore-forming [1].
The organism is of significant interest for its position in the tree of life within the small (2 type strains) family Beutenbergiaceae Zhi, et al, 2009 emend. Schumann et al. 2009 in the actinobacterial suborder Micrococcineae [2], which in addition to the genus Beutenbergia contains only the genus Salana [3,4] (Figure 1), also otherwise stated in a recent overview on the class Actinobacteria [2]. Here we present a summary classification and a set of features for B. cavernae strain HKI 0122T (Table 1), together with the description of the complete genome sequencing and annotation.
Figure 1
Figure 1
Phylogenetic tree of B. cavernae HKI 0122T and all type strains of the genus Beutenbergia, inferred from 1411 aligned characters [5,6] of the 16S rRNA sequence under the maximum likelihood criterion [7]. The tree was rooted with species from the genera (more ...)
Table 1
Table 1
Classification and general features of B. cavernae HKI 0122T based on the MIGS recommendations [9]
In addition to strain HKI 0122T, only one additional strain (HKI 0132) was isolated from the soil sample collected in the Reed Flute Cave near Guilin, Guangxi, China. HKI 0132 was also classified in the species B. cavernae [1]. No closely related isolates and uncultivated clones with more than 97% 16S rRNA gene sequence identity are recorded in the microbiological literature, nor can any phylotype from environmental samples or genomic surveys be directly linked to B. cavernae.
B. cavernae cells vary in shape and colonies grown on rich medium vary in color from cream to bright yellow. In young cultures, cells are irregular rods arranged in palisades, clusters or in pairs at an angle to give V-formations (Figure 2) [1]. Cells in stationary cultures are predominantly coccoid, occurring singly, in pairs, irregular clusters and short chains. During growth in complex media a rod-coccus growth cycle was observed [1]. B. cavernae grow well under aerobic and microaerophilic conditions, but not under anaerobic conditions [1]. The optimal growth temperature is 28°C [1].
Figure 2
Figure 2
Scanning electron micrograph of B. cavernae HKI 0122T
B. cavernae is able to degrade casein, esculin, gelatin and potato starch. Acids are produced from L-arabinose, D-cellobiose, dextrin, D-fructose, D-galactose, D-glucose, glycerol, inulin, maltose, D-mannose, D-raffinose, L-rhamnose, D-ribose, salicin, sucrose, starch, trehalose and D-xylose. There is no acid production from D-glucitol, lactose and D-mannitol. Nitrate is reduced to nitrite, H2S is produced [1].
Figure 1. shows the phylogenetic neighborhood of B. cavernae strain HKI 0122T in a 16S rRNA based tree. Analysis of the two identical 16S rRNA gene sequences in the genome of strain HKI differed by four nucleotides from the previously published 16S rRNA sequence generated from DSM 12333 (Y18378). The slight differences between the genome data and the reported 16S rRNA gene sequence is most likely due to sequencing errors in the previously reported sequence data .
Chemotaxonomy
The peptidoglycan of B. cavernae HKI 0122T contains D- and L-alanine, D- and L-glutamic acid and L-lysine, with the latter widely distributed among actinobacteria [1]. The strain possesses a type A4left angle bracket peptidoglycan with a diagnostic LLys←L-Glu interpeptide bridge, type A11.54 according to http://www.dsmz.de/microorganisms/. Glucose, mannose and galactose are the cell wall sugars [1]. The fatty acid profile of strain B. cavernae HKI 0122T is dominated by 13-methyl tetradecanoic (iso-C15:0; 43.7%) and 12-methyl tetradecanoic (anteiso-C15:0; 34.6%) saturated, branched chain acids. Other predominantly saturated fatty acids play a minor role in the cellular fatty acid composition of the strain: iso-C14:0 (0.9%), C14:0 (1.9%); C15:0 (0.9%) isoC16:0 (2.3%), C16:0 (6.8%), isoC17:0 (3.1%), anteiso-C17:0 (4.9%), und C18:1 (0.9%) [1]. Mycolic acids are not present [1]. MK-8(H4) is the major menaquinone, complemented by minor amounts of MK-8(H2), MK-8 and MK-9(H4) [1]. The combination of the LLys←L-Glu interpeptide bridge and MK-8(H4) as the dominating menaquinone is shared with the organisms from the neighboring genera Bogoriella and Georgenia. The polar lipids of strain HKI 0122T consist of phosphatidylinositol and diphosphatidylglycerol together with three yet unidentified phospholipids [1].
Genome project history
This organism was selected for sequencing on the basis of its phylogenetic position, and is part of the Genomic Encyclopedia of Bacteria and Archaea project. The genome project is deposited in the Genomes OnLine Database [8] and the complete genome sequence in GenBank (CP001618). Sequencing, finishing and annotation were performed by the DOE Joint Genome Institute (JGI). A summary of the project information is shown in Table 2.
Table 2
Table 2
Genome sequencing project information
Growth conditions and DNA isolation
B. cavernae HKI 0122T, DSM 12333, was grown in DSMZ medium 736 (Rich Medium) [13] at 28°C. DNA was isolated from 0.5-1 g of cell paste using Qiagen Genomic 500 DNA Kit (Qiagen, Hilden, Germany) with a modification of the standard protocol for cell lysis in first freezing for 20 min. (-70°C), then heating 5 min. (98°C), and cooling 15 min to 37°C; adding 1.5 ml lysozyme (standard: 0.3 ml, only), 1.0 ml achromopeptidase, 0.12 ml lysostaphine, 0.12 ml mutanolysine, 1.5 ml proteinase K (standard: 0.5 ml, only). Over night incubation at 35°C.
Genome sequencing and assembly
The genome was sequenced using a combination of Sanger and 454 sequencing platforms. All general aspects of library construction and sequencing performed at the JGI can be found at the JGI website. 454 Pyrosequencing reads were assembled using the Newbler assembler version 1.1.02.15 (Roche). Large Newbler contigs were broken into 5,256 overlapping fragments of 1000 bp and entered into the assembly as pseudo-reads. The sequences were assigned quality scores based on Newbler consensus q-scores with modifications to account for overlap redundancy and to adjust inflated q-scores. A hybrid 454/Sanger assembly was made using the parallel phrap assembler (High Performance Software, LLC). Possible mis-assemblies were corrected with Dupfinisher or transposon bombing of bridging clones [14]. Gaps between contigs were closed by editing in Consed, custom primer walking or PCR amplification. A total of 1627 Sanger finishing reads were produced to close gaps, to resolve repetitive regions, and to raise the quality of the finished sequence. The error rate of the completed genome sequence is less than 1 in 100,000. Together all sequence types provided 19.42x coverage of the genome.
Genome annotation
Genes were identified using Prodigal [15] as part of the Oak Ridge National Laboratory genome annotation pipeline, followed by a round of manual curation using the JGIGenePRIMP pipeline [16]. The predicted CDSs were translated and used to search the National Center for Biotechnology Information (NCBI) nonredundant database, UniProt, TIGRFam, Pfam, PRIAM, KEGG, COG, and InterPro databases. Additional gene prediction analysis and functional annotation was performed within the Integrated Microbial Genomes (IMG-ER) platform [17].
The genome is 4,669,183 bp long and comprises one main circular chromosome with a 73.1% GC content. (Table 3 and Figure 3). Of the 4278 genes predicted, 4225 were protein coding genes, and 53 RNAs. Twenty eight pseudogenes were also identified. The majority of the genes (74.3%) were assigned a putative function while the remaining ones were annotated as hypothetical proteins. The distribution of genes into COGs functional categories is presented in Table 4.
Table 3
Table 3
Genome Statistics
Figure 3
Figure 3
Graphical circular map of the genome. From outside to the center: Genes on forward strand (color by COG categories), Genes on reverse strand (color by COG categories), RNA genes (tRNAs green, rRNAs red, other RNAs black), GC content, GC skew
Table 4
Table 4
Number of genes associated with the 21 general COG functional categories
Acknowledgements
We would like to gratefully acknowledge the help of Katja Steenblock for growing B. cavernae cultures and Susanne Schneider for DNA extraction and quality analysis (both at DSMZ). This work was performed under the auspices of the US Department of Energy's Office of Science, Biological and Environmental Research Program, and by the University of California, Lawrence Berkeley National Laboratory under contract No. DE-AC02-05CH11231, Lawrence Livermore National Laboratory under Contract No. DE-AC52-07NA27344, and Los Alamos National Laboratory under contract No. DE-AC02-06NA25396, as well as German Research Foundation (DFG) INST 599/1-1.
1. Groth I, Schumann P, Schütze B, Augsten K, Kramer I, Stackebrandt E. Beutenbergia cavernae, gen. nov., sp. nov., a L-lysine-containing actinomycete isolated from a cave. Int J Syst Bacteriol 1999; 49:1733-1740. doi: 10.1099/00207713-49-4-1733. [PubMed] [Cross Ref]
2. Zhi XY, Li WJ, Stackebrandt E. An update of the structure and 16S rRNA gene sequence-based definition of higher ranks of the class Actinobacteria, with the proposal of two new suborders and four new families and embedded descriptions of the existing higher taxa. Int J Syst Evol Microbiol 2009; 59:589-608. doi: 10.1099/ijs.0.65780-0. [PubMed] [Cross Ref]
3. von Wintzingerode F, Göbel UB, Siddiqui RA, Rösick U, Schumann P, Frühling A, Rohde M, Pukall R, Stackebrandt E. Salana multivorans gen. nov., sp. nov., a novel actinobacterium isolated from an anaerobic bioreactor and capable of selenate reduction. Int J Syst Evol Microbiol 2001; 51:1653-1661. [PubMed]
4. Schumann P, Kampfer P, Busse HJ, Evtushenko LI. Proposed minimal standards for describing new genera and species of the suborder Micrococcineae. Int J Syst Evol Microbiol 2009; 59:1823-1849. doi: 10.1099/ijs.0.012971-0. [PubMed] [Cross Ref]
5. Lee C, Grasso C, Sharlow MF. Multiple sequence alignment using partial order graphs. Bioinformatics 2002; 18:452-464. doi: 10.1093/bioinformatics/18.3.452. [PubMed] [Cross Ref]
6. Castresana J. Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol 2000; 17:540-552. [PubMed]
7. Stamatakis A, Hoover P, Rougemont J. A rapid bootstrap algorithm for the RAxML web-servers. Syst Biol 2008; 57:758-771. doi: 10.1080/10635150802429642. [PubMed] [Cross Ref]
8. Liolios K, Mavromatis K, Tavernarakis N, Kyrpides NC. The Genomes OnLine Database (GOLD) in 2007: status of genomic and metagenomic projects and their associated metadata. Nucleic Acids Res 2008; 36:D475-D479. doi: 10.1093/nar/gkm884. [PMC free article] [PubMed] [Cross Ref]
9. Field D, Garrity G, Gray T, Morrison N, Selengut J, Sterk P, Tatusova T, Thomson N, Allen MJ, Angiuoli SV. Towards a richer description of our complete collection of genomes and metagenomes: the “Minimum Information about a Genome Sequence” (MIGS) specification. Nat Biotechnol 2008; 26:541-547. doi: 10.1038/nbt1360. [PMC free article] [PubMed] [Cross Ref]
10. Stackebrandt E, Rainey FA, Ward-Rainey NL. Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 1997; 47:479-491. doi: 10.1099/00207713-47-2-479. [Cross Ref]
11. Biological Agents. Technical rules for biological agents www.baua.de TRBA 466.
12. Ashburner M, Ball CA, Blake JA, Botstein D, Butler H, Cherry JM, Davis AP, Dolinski K, Dwight SS, Eppig JT, et al. Gene ontology: tool for the unification of biology. The Gene Ontology Consortium. Nat Genet 2000; 25:25-29. doi: 10.1038/75556. [PMC free article] [PubMed] [Cross Ref]
13. Yamada K, Komagata K. Taxonomic studies on coryneform bacteria IV. Morphological, cultural, biochemical, and physiological characteristics. J Gen Appl Microbiol 1972; 18:399-416. doi: 10.2323/jgam.18.399. [Cross Ref]
14. Sims D, Brettin T, Detter JC, Han C, Lapidus A, Copeland A, Glavina Del Rio T, Nolan M, Chen F, Lucas S, et al. Complete genome of Kytococcus sedentarius type strain (strain 541T). Stand Genomic Sci 2009; 1:12-20. doi: 10.4056/sigs.761. [PMC free article] [PubMed] [Cross Ref]
16. Markowitz VM, Mavromatis K, Ivanova NN, Chen IMA, Kyrpides NC. Expert Review of Functional Annotations for Microbial Genomes. Bioinformatics 2009; 25:2271-2278. doi: 10.1093/bioinformatics/btp393. [PubMed] [Cross Ref]
17. Pati A, Ivanova N, Mikhailova N, Ovchinikova G, Hooper SD, Lykidis A, Kyrpides NC. GenePRIMP: A Gene Prediction Improvement Pipeline for microbial genomes. in preparation. Nat Methods 2010; 7:455-457. doi: 10.1038/nmeth.1457. [PubMed] [Cross Ref]
Articles from Standards in Genomic Sciences are provided here courtesy of
BioMed Central