PMCCPMCCPMCC

Search tips
Search criteria 

Advanced

Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
 
From:
Obstet Gynecol. Author manuscript; available in PMC 2011 June 1.
Published in final edited form as:
Obstet Gynecol. 2010 June; 115(6): 1209–1216.
doi: 10.1097/AOG.0b013e3181e01415

Table 1

Comparison of HSV PCR Results by Rapid PCR and TaqMan Assay using 3 Different Cut-off Strategies.

Rapid PCR
Interpretation
Strategy

Cut-off
(c/ml)
Rapid
PCR
Result

TaqMan

Positive

Result

Negative


Sensitivity


Specificity
A50Positive2061997.296.2
Negative6485

BPositive206497.299.2
Negative6500

CPositive206597.299.2
Negative6499

A100Positive2061297.297.6
Negative6492
BPositive206497.299.2
Negative6500
CPositive206697.299.2
Negative6500

A150Positive206897.298.4
Negative6496
BPositive205296.799.6
Negative7502
CPositive206497.299.2
Negative6500

A250Positive203495.899.2
Negative9500
BPositive199193.999.8
Negative13503
CPositive203395.899.4
Negative9501

A500Positive199193.999.8
Negative13503
BPositive197092.9100.0
Negative15504
CPositive199193.999.8
Negative13503

Strategy A averages the quantitative values of both wells of the rapid test into a single value that is compared to the cut-off value to determine test positivity.

Strategy B uses the quantitative data from both wells separately with both wells required to exceed cut-off for the specimen to be considered positive.

Strategy C compares the average of both wells after excluding specimens with one positive(defined as >0 c/ml) and one negative to the stated cut-off values.