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Logo of nihpaAbout Author manuscriptsSubmit a manuscriptHHS Public Access; Author Manuscript; Accepted for publication in peer reviewed journal;
Nature. Author manuscript; available in PMC 2011 July 6.
Published in final edited form as:
Published online 2010 November 28. doi:  10.1038/nature09635
PMCID: PMC3030448

Reversing EphB2 depletion rescues cognitive functions in Alzheimer model


Amyloid-β oligomers may cause cognitive deficits in Alzheimer's disease by impairing neuronal NMDA-type glutamate receptors, whose function is regulated by the receptor tyrosine kinase EphB2. Here we show that amyloid-β oligomers bind to the fibronectin repeats domain of EphB2 and trigger EphB2 degradation in the proteasome. To determine the pathogenic importance of EphB2 depletions in Alzheimer's disease and related models, we used lentiviral constructs to reduce or increase neuronal expression of EphB2 in memory centres of the mouse brain. In nontransgenic mice, knockdown of EphB2 mediated by short hairpin RNA reduced NMDA receptor currents and impaired long-term potentiation in the dentate gyrus, which are important for memory formation. Increasing EphB2 expression in the dentate gyrus of human amyloid precursor protein transgenic mice reversed deficits in NMDA receptor-dependent long-term potentiation and memory impairments. Thus, depletion of EphB2 is critical in amyloid-β-induced neuronal dysfunction. Increasing EphB2 levels or function could be beneficial in Alzheimer's disease.

Soluble amyloid-β oligomers may contribute to learning and memory deficits in Alzheimer's disease by inhibiting NMDA-receptor-dependent long-term potentiation (LTP)13, thought to underlie memory formation4. In Alzheimer's disease, hippocampal NMDA-receptor-subunit levels are reduced5, and protein levels and the phosphorylation status of NMDA-receptor subunits NR1, NR2A and NR2B correlate with cognitive performance6. Human amyloid precursor protein (hAPP) transgenic mice with high brain levels of amyloid-β oligomers have reduced hippocampal levels of tyrosine-phosphorylated NMDA receptors and key components of NMDA-receptor-dependent signalling pathways7,8. Alzheimer's disease patients and hAPP mice have hippocampal depletions of the receptor tyrosine kinase EphB29, which regulates NMDA-receptor trafficking and function by interacting with NMDA receptors and Src-mediated tyrosine phosphorylation1013. EphB2 regulates NMDA-receptor-dependent Ca2+ influx and downstream transcription factors involved in LTP formation12, such as Fos, which is depleted in the dentate gyrus of hAPP mice. Mice lacking EphB210,14 or Fos15 have impaired NMDA-receptor-dependent LTP and memory deficits. We hypothesized that EphB2 depletion in Alzheimer's disease-related models is caused by amyloid-β oligomers and that reductions in EphB2 contribute to amyloid-β-induced deficits in synaptic plasticity and cognitive functions (Supplementary Fig. 1). Here we confirm these hypotheses and show that reversing EphB2 depletion in the dentate gyrus of hAPP mice reverses LTP and memory impairments.

Amyloid-β oligomers bind to EphB2

To determine if amyloid-β oligomers interact directly with EphB2, we measured binding of biotinylated synthetic amyloid-β1–42 oligomers to a purified recombinant EphB2–Fc chimaera. Biotinylated amyloid-β oligomers and EphB2–Fc were pulled down together by avidin agarose beads (Supplementary Fig. 2a, b) and co-immunoprecipitated under cell-free conditions (Supplementary Fig. 2c, d). EphB2 and amyloid-β oligomers also co-immunoprecipitated from homogenates of primary neurons (Supplementary Fig. 2e–g). Thus, amyloid-β oligomers may interact directly with the extracellular region of EphB2.

This region comprises a ligand-binding (LB) domain, a cysteine-rich (CR) domain, and a fibronectin type III repeats (FN) domain (Fig. 1a). To determine which domain mediates the interaction with amyloid-β oligomers, we generated EphB2–GST deletion mutants lacking the LB domain (DLB-EphB2) or the FN domain (DFN-EphB2) (Fig. 1a). Amyloid-β oligomers bound to FL-EphB2 and DLB-EphB2 but not DFN-EphB2 (Fig. 1b, c), indicating that the FN domain is critical for their interaction with EphB2.

Figure 1
Amyloid-β oligomers bind to the fibronectin repeats domain of EphB2 and cause degradation of EphB2 in the proteasome

Deleting FN domain did not affect trafficking of EphB2 to the cell surface (Supplementary Fig. 3a). FL-EphB2 and DFN-EphB2 both phosphorylated the NMDA-receptor subunit NR1 after stimulation with the EphB2 ligand, Fc-ephrin-B2 (Supplementary Fig. 3b–d). Thus, deleting the FN domain did not eliminate the kinase function of EphB2. Deleting the LB domain prevented Fc-ephrin-B2-induced phosphorylation of NR1 (Supplementary Fig. 3b–d).

Mechanisms of amyloid-β-induced EphB2 depletion

At 3–4 but not 2 months of age, EphB2 messenger RNA (mRNA) and protein levels in the hippocampus were lower in hAPP mice than in nontransgenic controls, and were lower in humans with Alzheimer's disease than in nondemented controls (data not shown), consistent with previous findings9.

As reported by others16, we observed a doublet of putative EphB2 carboxy-terminal fragments (CTFs) of 45–50 kDa in hippocampi of hAPP mice and nontransgenic controls on western blots (not shown). Relative to nontransgenic controls, hAPP mice showed a comparable decrease in CTFs and FL-EphB2 (not shown) and no difference in the ratio of CTF1+CTF2:FL−EphB2 (hAPP, 2.7 ± 0.36; nontransgenic, 2.3 ± 0.59; P = 0.55 by t test). Thus, pathologically raised levels of amyloid-β do not affect EphB2 cleavage into CTFs.

Treating primary neuronal cultures from wild-type rats with naturally secreted amyloid-β oligomers caused severe EphB2 depletions by 3 days (Fig. 1d–f). Amyloid-β oligomers reduced EphB2 mRNA levels (Fig. 1g), but the reduction was subtle and unlikely to account for the severe EphB2 protein depletion.

Amyloid-β-induced depletion of EphB2 was blocked by the proteasome inhibitor lactacystin (Fig. 1h, i). Bafilomycin, an inhibitor of endosomal acidification, had no effect (Supplementary Fig. 4b, c). Compared with amyloid-β treatment alone, treatment of cells with lactacystin alone or together with amyloid-β increased ubiquitinated EphB2 (Supplementary Fig. 4a). These results indicate that amyloid-β depletes neuronal EphB2 mainly by enhancing its proteasomal degradation.

EphB2 depletion impairs synaptic plasticity

To determine if EphB2 depletion interferes with NMDA-receptor-dependent functions, we generated lentiviral vectors expressing green fluorescent protein (GFP) and anti-EphB2 shRNA (Lenti-sh-EphB2–GFP) or scrambled control shRNA (Lenti-sh-SCR–GFP). In neuronal cultures, Lenti-sh-EphB2–GFP reduced EphB2 mRNA and protein levels (Fig. 2a, b) and surface levels of NR1 (Fig. 2c–e). In cultures co-infected with a mutant EphB2 construct whose mRNA is resistant to sh-EphB2 (Lenti-mut-EphB2–Flag) and Lenti-sh-EphB2–GFP, EphB2 and surface NR1 were not reduced (Supplementary Fig. 5), excluding an off-target effect. Next we examined the effects of sh-EphB2 on expression of the immediate-early gene c-fos, which depends on NMDA receptors and is regulated by EphB212. Anti-EphB2 shRNA prevented Fc-ephrin-B2-induced increases in Fos expression in neurons expressing wild-type EphB2, but not in neurons expressing mutant EphB2 (Fig. 2f). Thus, depleting EphB2 reduces surface NR1 expression and impairs NMDA-receptor-dependent gene expression.

Figure 2
Knockdown of EphB2 reduces surface NR1 levels and Fc-ephrin-B2-dependent Fos expression

To explore whether EphB2 depletion accounts for LTP deficits in hAPP mice8, we reduced EphB2 in the dentate gyrus of nontransgenic mice. Although granule cells are not very susceptible to degeneration in Alzheimer's disease, perforant path to granule cell synapses are affected early and severely17,18.

Two anti-EphB2 shRNAs reduced EphB2 mRNA and protein levels in neuronal culture (Supplementary Fig. 6). Mice injected with lentiviral vectors expressing sh-EphB2-308–GFP (Fig. 3a, b) or sh-EphB2-306–GFP (Supplementary Fig. 7a, b) had lower EphB2 mRNA levels in the dentate gyrus than controls. Transduction efficiencies (Supplementary Fig. 8) were 50–74% (mean ± s.e.m., 62.4 ± 6.2; n = 7 mice), consistent with other reports19,20.

Figure 3
Knockdown of EphB2 reduces LTP in dentate gyrus granule cells of nontransgenic mice

Field (Fig. 3c) and whole-cell patch-clamp recordings (Fig. 3e) from dentate gyrus granule cells in acute hippocampal slices from Lenti-sh-EphB2–GFP-injected nontransgenic mice revealed prominent LTP deficits similar to those in untreated hAPP J20 (Fig. 3d, f) and other lines of hAPP mice21,22. Lenti-sh-SCR–GFP-injected nontransgenic mice had robust LTP in the dentate gyrus (Fig. 3c, e). Whole-cell recordings from individual GFP-negative granule cells in Lenti-sh-ephB2–GFP-injected mice revealed no LTP deficits, compared with GFP-negative granule cells in untreated nontransgenic mice and GFP-positive granule cells in Lenti-sh-SCR–GFP-injected mice (P > 0.1 by repeated-measures ANOVA, n = 6 neurons from 3 mice per group; data not shown).

EphB2 depletion reduces synaptic strength

LTP at the medial perforant path to granule cell synapse depends on NMDA-receptor activity23. We determined whether impaired synaptic plasticity in sh-EphB2-treated nontransgenic and untreated hAPP mice is related to a selective impairment of these glutamate receptors. NMDA-receptor-mediated, but not a-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor–mediated synaptic transmission strength at this synapse was affected in sh-EphB2-treated nontransgenic mice (Fig. 3g) and untreated hAPP mice (Fig. 3h), as determined by field recordings and analysis of input-output (I/O) curves. These alterations markedly reduced ratios of NMDA-receptor- to AMPA-receptor-mediated synaptic strength in both groups (Fig. 3j). Similar results were obtained by whole-cell recordings from individual granule cells (Fig. 3i, k). To exclude a contribution of alterations in AMPA-receptor currents to the altered ratios, we recorded pharmacologically isolated, AMPA-receptor-mediated miniature excitatory synaptic currents (mEPSCs). The four groups of mice had comparable mEPSC peak amplitudes (Supplementary Fig. 9). Thus, like amyloid-β, EphB2 depletion probably reduces LTP by impairing NMDA-receptor function.

EphB2 rescues synaptic functions in hAPP mice

To determine if increasing EphB2 expression in the dentate gyrus of hAPP mice reverses LTP deficits, we used a lentivirus expressing EphB2–Flag (Lenti-EphB2–Flag). Lenti-EphB2–Flag-treated hAPP and nontransgenic mice had comparable EphB2–Flag expression levels in the dentate gyrus (Fig. 4a and Supplementary Fig. 10). Lenti-empty-treated nontransgenic mice and Lenti-EphB2–Flag-treated hAPP mice had comparable dentate gyrus levels of total (endogenous and exogenous) EphB2 (Fig. 4b), indicating that EphB2 levels in hAPP mice were normalized. EphB2 levels were lower in Lenti-empty-injected hAPP mice and higher in Lenti-EphB2–Flag-injected nontransgenic mice (Fig. 4b). Increasing dentate gyrus EphB2 levels reversed LTP deficits in two independent cohorts of hAPP mice but did not alter LTP in nontransgenic mice (Fig. 4c).

Figure 4
Increasing EphB2 expression rescues synaptic plasticity in hAPP mice

Lenti-EphB2–Flag-treated mice showed a trend toward lower amyloid-β levels in the dentate gyrus (Supplementary Fig. 11), but this trend did not reach statistical significance. At analysis, hAPP mice were 4–5-months old and had not yet formed plaques, excluding EphB2 effects on plaque formation. To determine if LTP rescue was due to improved NMDA-receptor function, we again measured AMPA-receptor- and NMDA-receptor-mediated synaptic strength. Increasing EphB2 levels in the dentate gyrus of hAPP mice reversed deficits in NMDA-receptor-mediated synaptic strength without changing AMPA-receptor-mediated synaptic strength (Fig. 4d, e), normalizing the balance between them (Fig. 4f). Overexpressing EphB2 did not alter NMDA-receptor- or AMPA-receptor-mediated synaptic strength in nontransgenic mice (Fig. 4d–f).

Increasing EphB2 expression in granule cells did not reverse impairments in paired pulse modification at perforant path to granule cell synapses (Supplementary Fig. 12a) or in synaptic strength at Schaffer collateral to CA1 pyramidal cell synapses (Supplementary Fig. 12b, c).

EphB2 ameliorates cognitive deficits in hAPP mice

To determine if increasing EphB2 levels in the dentate gyrus also reverses learning and memory deficits in hAPP mice2427, we injected Lenti-EphB2–Flag or Lenti-empty bilaterally into the dentate gyrus of hAPP and nontransgenic mice and analysed them behaviourally 2 months later.

Spatial learning and memory in the Morris water maze is strongly affected by dentate gyrus impairments28. In the spatial, hidden-platform component, Lenti-EphB2–Flag-treated but not Lenti-empty-treated hAPP mice performed at control levels (Fig. 5a, b). Overexpressing EphB2 did not alter learning in nontransgenic mice (Fig. 5a, b). All groups of mice learned similarly well in the cued-platform component (data not shown).

Figure 5
Increasing EphB2 expression in the dentate gyrus ameliorates learning and memory deficits in hAPP mice

In a probe trial, Lenti-empty-treated but not Lenti-EphB2–Flag-treated hAPP mice took longer to reach the original platform location than Lenti-empty-treated nontransgenic controls (Fig. 5c). Lenti-EphB2–Flag-treated nontransgenic mice performed slightly worse than Lenti-empty-treated nontransgenic mice (Fig. 5c) (P = 1.0 by one-way ANOVA and Bonferroni post-hoc test).

In the novel object recognition test, Lenti-EphB2-treated but not Lenti-empty-treated hAPP mice spent more time exploring the novel object (Fig. 5d). In the novel place recognition test, Lenti-EphB2-treated but not Lenti-empty-treated hAPP mice spent more time exploring the object whose location had changed (Fig. 5e). Thus, increasing EphB2 expression in the dentate gyrus of hAPP mice ameliorates deficits in both spatial and nonspatial learning and memory.

Finally, we assessed passive avoidance learning, which depends at least partly on hippocampal functions29,30. During training, escape latencies were similar across groups (Fig. 5f). However, 24 h later, Lenti-empty-treated hAPP mice were severely impaired, whereas all other groups performed well (Fig. 5f). Increasing dentate gyrus EphB2 levels in hAPP mice did not reverse behavioural deficits that were probably caused by impairments of other brain regions, including hyperactivity in the open field and disinhibition in the elevated plus maze (Supplementary Fig. 13).


Our study shows that EphB2 depletion contributes to amyloid-β-induced neuronal deficits and cognitive dysfunction. Reducing neuronal EphB2 levels caused functional deficits similar to those caused by amyloid-β, including deficits in NMDA-receptor-dependent synaptic strength and gene expression and impaired LTP and memory. Increasing neuronal EphB2 levels in hAPP mice reversed these deficits, indicating that EphB2 impairment is necessary and sufficient to elicit them and that increasing EphB2 activity counteracts amyloid-β-induced neuronal dysfunction. Consistent with a previous report9, EphB2 depletion in memory-related brain regions was detected not only in hAPP mice, but also in humans with Alzheimer's disease, underlining the potential clinical relevance of our findings. Our data further indicate that the depletion of EphB2 by amyloid-β oligomers involves direct binding of amyloid-β oligomers to the FN repeats domain of EphB2 and EphB2 degradation in the proteasome. Reduction of EphB2 mRNA may have an additional role.

Our results and those of others indicate that neuronal EphB2 depletion causes deficits in learning and memory by impairing NMDA-receptor functions (Supplementary Fig. 1). EphB2 modulates NMDA receptors by tyrosine phosphorylation and recruits active NMDA receptors to excitatory synapses1012. EphB2-deficient mice have LTP deficits10,14 and fewer NR1 subunits in the postsynaptic density10. Our results are consistent with these findings, although LTP deficits after shRNA knockdown of EphB2 in adult nontransgenic mice were more severe than those in EphB2-deficient mice. Other members of the large Eph family might partially compensate for EphB2 ablation during early development. The more severe deficits after acute EphB2 knockdown in adults probably reflect the lack of such compensation. Modulation of other LTP-related proteins also results in different outcomes, depending on when it is initiated31.

Amyloid-β may impair LTP by inducing internalization of NMDA receptors32,33. We found that depletion of EphB2 contributes to the amyloid-β-induced decrease in NMDA receptors and that increasing EphB2 expression markedly improves LTP and memory even in the presence of high amyloid-β levels. Increased EphB2 levels probably increase surface NMDA-receptor expression. Indeed, increasing neuronal EphB2 expression reversed amyloid-β-induced deficits in NMDA-receptor-mediated synaptic strength.

Opposition of amyloid-β-induced surface depletion of NMDA receptors is the most parsimonious interpretation of the EphB2-mediated rescue effects (Supplementary Fig. 1). However, amyloid-β may impair LTP and memory through alternative processes, and increased expression of EphB2 may counteract amyloid-β effects also through downstream signalling mechanisms.

Manipulating individual functional hubs of neurons can profoundly affect a larger network34,35. Even manipulating an individual neuron can affect the global brain state36. Thus, improving the function of a subset of neurons might allow an impaired brain region to better support specific behaviours. The current study supports this hypothesis: increasing EphB2 expression in a subset of granule cells improved dentate gyrus LTP and learning and memory in hAPP mice. It remains to be determined whether EphB2 depletions contribute to amyloid-β-dependent impairments in other brain regions and whether increasing neuronal EphB2 levels in these regions is tolerated as well as it was in the dentate gyrus. If so, pharmacological treatments might be used to increase EphB2 expression or activity. Our results indicate additional entry points for interventions (Supplementary Fig. 1). For example, it may be possible to identify small molecules that block the binding of amyloid-β oligomers to EphB2's FN repeats domain, prevent proteasomal degradation of EphB2, or improve its interactions with NMDA receptors. 1555 (1997).



Unless indicated otherwise, all data reported in this paper were obtained in blind-coded experiments, in which the investigators who obtained the data were unaware of the specific genotype and treatment of mice, brain slices and cell cultures. For number of mice, slices and cell cultures analysed in each experiment, refer to Supplementary Table 1. For experimental details related to each figure legend, refer to Supplementary Table 2.

Experimental models

Heterozygous transgenic and nontransgenic mice were from hAPP line J207,8,37,38. Primary neuronal cultures from wild-type rats were treated with medium conditioned by CHO cells that do or do not produce human amyloid-β oligomers (Supplementary Figs 14 and 15 and refs 39, 40.).

Experimental manipulations

Lentiviral constructs directing neuronal expression of no transgene products, EphB2-Flag, or GFP in combination with anti-EphB2 shRNAs or scrambled control shRNA were injected stereotactically into the dentate gyrus of mice20,41. Neuronal cultures were infected with some of these constructs and stimulated with Fc-ephrin-B2 or Fc control12,42.

Outcome measures

The interaction between biotinylated or naturally secreted amyloid-β oligomers and EphB2 was assessed under cell-free conditions and in neuronal cultures of primary neurons or HEK cells by pull-down with avidin agarose beads43 or immunoprecipitation and western blot44. EphB2 and NR1 levels in brain tissues or neuronal cultures were determined by immunoprecipitation and western blot or western blot alone44. Corresponding transcripts were measured by quantitative polymerase chain reaction with reverse transcription (RT-qPCR). Fos expression in neuronal cultures was determined by western blot44. Field recordings8 or whole-cell patch-clamp recordings45 from acute hippocampal slices were used to determine synaptic strength (fEPSP I/O relationships; mediated by either AMPA receptors or NMDA receptors), synaptic plasticity (LTP), and NMDA-receptor:AMPA-receptor ratios of EPSCs at the medial perforant path to dentate gyrus granule cell synapses. Learning and memory were assessed in the Morris water maze, novel object recognition test, novel place recognition test, and passive avoidance test4649. Amyloid-β levels in the dentate gyrus of hAPP-J20 mice were determined by ELISA50.

Supplementary Material

Supplementary Material


We thank I. Ethell for the plasmid encoding the Flag-tagged EphB2 receptor; D. J. Selkoe and D. Walsh for CHO-7PA2 cells; S. Finkbeiner for the plasmid encoding the NMDA receptor subunit NR1; J. Palop for comments; H. Solanoy, M. Thwin and X. Wang for technical support; G. Howard and S. Ordway for editorial review; J. Carroll for preparation of graphics; and M. Dela Cruz for administrative assistance. The study was supported by NIH grants AG011385, AG022074 and NS041787 to L.M., a fellowship from the McBean Family Foundation to M.C., and the National Center for Research Resources Grant RR18928-01 to the Gladstone Institutes.


Full Methods and any associated references are available in the online version of the paper at


1. Walsh DM, Selkoe DJ. Deciphering the molecular basis of memory failure in Alzheimer's disease. Neuron. 2004;44:181–193. [PubMed]
2. Shankar GM, et al. Amyloid-β protein dimers isolated directly from Alzheimer's brains impair synaptic plasticity and memory. Nature Med. 2008;14:837–842. [PMC free article] [PubMed]
3. Kamenetz F, et al. APP processing and synaptic function. Neuron. 2003;37:925–937. [PubMed]
4. Malenka RC, Bear MF. LTP and LTD: an embarrassment of riches. Neuron. 2004;44:5–21. [PubMed]
5. Ikonomovic MD, et al. Distribution of glutamate receptor subunit NMDAR1 in the hippocampus of normal elderly and patients with Alzheimer's disease. Exp. Neurol. 1999;160:194–204. [PubMed]
6. Sze C, Bi H, Kleinschmidt-DeMasters BK, Filley CM, Martin LJ. N-Methyl-D-aspartate receptor subunit proteins and their phosphorylation status are altered selectively in Alzheimer's disease. J. Neurol. Sci. 2001;182:151–159. [PubMed]
7. Palop JJ, et al. Vulnerability of dentate granule cells to disruption of Arc expression in human amyloid precursor protein transgenic mice. J. Neurosci. 2005;25:9686–9693. [PubMed]
8. Palop JJ, et al. Aberrant excitatory neuronal activity and compensatory remodeling of inhibitory hippocampal circuits in mouse models of Alzheimer's disease. Neuron. 2007;55:697–711. [PubMed]
9. Simon AM, et al. Early changes in hippocampal Eph receptors precede the onset of memory decline in mouse models of Alzheimer's disease. J. Alzheimers Dis. 2009;17:773–786. [PubMed]
10. Henderson JT, et al. The receptor tyrosine kinase EphB2 regulates NMDA-dependent synaptic function. Neuron. 2001;32:1041–1056. [PubMed]
11. Dalva MB, et al. EphB receptors interact with NMDA receptors and regulate excitatory synapse formation. Cell. 2000;103:945–956. [PubMed]
12. Takasu MA, Dalva MB, Zigmond RE, Greenberg ME. Modulation of NMDA receptor-dependent calcium influx and gene expression through EphB receptors. Science. 2002;295:491–495. [PubMed]
13. Chen Y, Fu AK, Ip NY. Bidirectional signaling of ErbB and Eph receptors at synapses. Neuron Glia Biol. 2008;4:211–221. [PubMed]
14. Grunwald IC, et al. Kinase-independent requirement of EphB2 receptors in hippocampal synaptic plasticity. Neuron. 2001;32:1027–1040. [PubMed]
15. Fleischmann A, et al. Impaired long-term memory and NR2A-type NMDA receptor-dependent synaptic plasticity in mice lacking c-Fos in the CNS. J. Neurosci. 2003;23:9116–9122. [PubMed]
16. Litterst C, et al. Ligand binding and calcium influx induce distinct ectodomain/γ-secretase-processing pathways of EphB2 receptor. J. Biol. Chem. 2007;282:16155–16163. [PubMed]
17. Wakabayashi K, Honer WG, Masliah E. Synapse alterations in the hippocampalentorhinal formation in Alzheimer's disease with and without Lewy body disease. Brain Res. 1994;667:24–32. [PubMed]
18. Scheff SW, Price DA. Alzheimer's disease-related alterations in synaptic density: neocortex and hippocampus. J. Alzheimers Dis. 2006;9:101–115. [PubMed]
19. Mueller-Steiner S, et al. Anti-amyloidogenic and neuroprotective functions of cathepsin B: implications for Alzheimer's disease. Neuron. 2006;51:703–714. [PubMed]
20. Sun B, et al. Imbalance between GABAergic and glutamatergic transmissions impairs adult neurogenesis in an animal model of Alzheimer's disease. Cell Stem Cell. 2009;5:624–633. [PMC free article] [PubMed]
21. Shemer I, et al. Non-fibrillar β-amyloid abates spike-timing-dependent synaptic potentiation at excitatory synapses in layer 2/3 of the neocortex by targeting postsynaptic AMPA receptors. Eur. J. Neurosci. 2006;23:2035–2047. [PubMed]
22. Ashe KH, Zahs KR. Probing the biology of Alzheimer's disease in mice. Neuron. 2010;66:631–645. [PMC free article] [PubMed]
23. Colino A, Malenka RC. Mechanisms underlying induction of long-term potentiation in rat medial and lateral perforant paths in vitro. J. Neurophysiol. 1993;69:1150–1159. [PubMed]
24. Harris JA, et al. Many neuronal and behavioral impairments in transgenic mouse models of Alzheimer's disease are independent of caspase cleavage of the amyloid precursor protein. J. Neurosci. 2010;30:372–381. [PMC free article] [PubMed]
25. Sanchez-Mejia RO, et al. Phospholipase A2 reduction ameliorates cognitve deficits in mouse model of Alzheimer's disease. Nature Neurosci. 2008;11:1311–1318. [PMC free article] [PubMed]
26. Meilandt WJ, et al. Enkephalin elevations contribute to neuronal and behavioral impairments in a transgenic mouse model of Alzheimer's disease. J. Neurosci. 2008;28:5007–5017. [PMC free article] [PubMed]
27. Roberson ED, et al. Reducing endogenous tau ameliorates amyloid b-induced deficits in an Alzheimer's disease mouse model. Science. 2007;316:750–754. [PubMed]
28. Nguyen PV, Abel T, Kandel ER, Bourtchouladze R. Strain-dependent differences in LTP and hippocampus-dependent memory in inbred mice. Learn. Mem. 2000;7:170–179. [PubMed]
29. Nakajima R, et al. Comprehensive behavioral phenotyping of calpastatin-knockout mice. Mol. Brain. 2008;1:7. [PMC free article] [PubMed]
30. Potter MC, et al. Reduction of endogenous kynurenic acid formation enhances extracellular glutamate, hippocampal plasticity, and cognitive behavior. Neuropsychopharmacology. 2010;35:1734–1742. [PMC free article] [PubMed]
31. Terashima A, et al. An essential role for PICK1 in NMDA receptor-dependent bidirectional synaptic plasticity. Neuron. 2008;57:872–882. [PMC free article] [PubMed]
32. Snyder EM, et al. Regulation of NMDA receptor trafficking by amyloid-β Nature Neurosci. 2005;8:1051–1058. [PubMed]
33. Kurup P, et al. Aβ-mediated NMDA receptor endocytosis in Alzheimer's disease involves ubiquitination of the tyrosine phosphatase STEP61. J. Neurosci. 2010;30:5948–5957. [PMC free article] [PubMed]
34. Bonifazi P, et al. GABAergic hub neurons orchestrate synchrony in developing hippocampal networks. Science. 2009;326:1419–1424. [PubMed]
35. Han JH, et al. Selective erasure of a fear memory. Science. 2009;323:1492–1496. [PubMed]
36. Li CY, Poo MM, Dan Y. Burst spiking of a single cortical neuron modifies global brain state. Science. 2009;324:643–646. [PMC free article] [PubMed]
37. Rockenstein EM, et al. Levels and alternative splicing of amyloid β protein precursor (APP) transcripts in brains of transgenic mice and humans with Alzheimer's disease. J. Biol. Chem. 1995;270:28257–28267. [PubMed]
38. Mucke L, et al. High-level neuronal expression of Ab1–42 in wild-type human amyloid protein precursor transgenic mice: synaptotoxicity without plaque formation. J. Neurosci. 2000;20:4050–4058. [PubMed]
39. Koo EH, Squazzo SL. Evidence that production and release of amyloid β-protein involves the endocytic pathway. J. Biol. Chem. 1994;269:17386–17389. [PubMed]
40. Walsh DM, et al. Naturally secreted oligomers of amyloid b protein potently inhibit hippocampal long-term potentiation in vivo. Nature. 2002;416:535–539. [PubMed]
41. Franklin KBJ, Paxinos G. The Mouse Brain in Stereotaxic Coordinates. Academic; 1997.
42. Xia Z, Dudek H, Miranti CK, Greenberg ME. Calcium influx via the NMDA receptor induces immediate early gene transcription by a MAP kinase/ERK-dependent mechanism. J. Neurosci. 1996;16:5425–5436. [PubMed]
43. Laurén J, Gimbel DA, Nygaard HB, Gilbert JW, Strittmatter SM. Cellular prion protein mediates impairment of synaptic plasticity by amyloid-β oligomers. Nature. 2009;457:1128–1132. [PMC free article] [PubMed]
44. Alfa Cisse M, et al. M1 and M3 muscarinic receptors control physiological processing of cellular prion by modulating Alzheimer's disease AM17 phosphorylation and activity. J. Neurosci. 2007;27:4083–4092. [PubMed]
45. Wu J, Rush A, Rowan MJ, Anwyl R. NMDA receptor- and metabotropic glutamate receptor-dependent synaptic plasticity induced by high frequency stimulation in the rat dentate gyrus in vitro. J. Physiol. (Lond.) 2001;533:745–755. [PubMed]
46. Raber J, et al. Hypothalamic-pituitary-adrenal function in Apoe−/− mice: possible role in behavioral and metabolic alterations. J. Neurosci. 2000;20:2064–2071. [PubMed]
47. Raber J, LeFevour A, Buttini M, Mucke L. Androgens protect against Apolipoprotein E4-induced cognitive deficits. J. Neurosci. 2002;22:5204–5209. [PubMed]
48. Dere E, Huston JP, De Souza Silva MA. Episodic-like memory in mice: simultaneous assessment of object, place and temporal order memory. Brain Res. Protoc. 2005;16:10–19. [PubMed]
49. Benice T, Rizk A, Kohama S, Pfankuch T, Raber J. Sex-differences in age-related cognitive decline in C57BL/6J mice associated with increased brain microtubule-associated protein 2 and synaptophysin immunoreactivity. Neuroscience. 2006;137:413–423. [PubMed]
50. Johnson-Wood K, et al. Amyloid precursor protein processing and Ab42 deposition in a transgenic mouse model of Alzheimer disease. Proc. Natl Acad. Sci. USA. 94:1550. [PubMed]