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Logo of jbcThe Journal of Biological Chemistry
 
From:
Published online 2010 November 24. doi: 10.1074/jbc.M110.148726

FIGURE 5.

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Inhibition of miR-217 + miR-377 increases HO-1 protein expression. Western blot of primary HUVEC cell samples that were treated for 1 h with 20 μm hemin followed by an additional 24-h incubation in growth medium 36 h after nucleofection with the miRIDIAN hairpin inhibitor against miRNA-217 and miRIDIAN hairpin inhibitor against miR-377. Bar graph below Western blots represents densitometry of HO-1 protein expression normalized to GAPDH protein expression, in which n = 3 independent trials. *, p < 0.05 compared with hemin-treated cells using a one-way ANOVA with Bonferroni t test.

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