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Logo of jbcThe Journal of Biological Chemistry
 
From:
Published online 2010 November 24. doi: 10.1074/jbc.M110.148726

FIGURE 2.

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miR-217 and miR-377 interact with the HMOX1-3′ UTR. Combinations of the three 3′ UTR luciferase reporter vectors containing the HMOX1–3′ UTR or a mutation in the proposed miR-217 binding site or the proposed miR-377 binding site HMOX1–3′ UTR was transfected into HEK 293 cells along with pcDNATM6.2-GW/EmGFP-miR-217 (miR-217), pcDNATM6.2-GW/EmGFP-miR-377 (miR-377), scrambled (scrmiR) controls pcDNATM6.2-GW/EmGFP-scrmiR-217 (scrmiR-217), and hsa-scrmir-377 (scrmiR-377). Twenty-four hours after transfection a luciferase assay was performed. Results demonstrate that miR-377 alone, and the combination of miR-217 and miR-377 significantly (*, p < 0.05) decreased luciferase reporter activity when compared with reporter alone (Kruskal-Wallis One Way Analysis of Variance on Ranks with Student-Newman-Keuls multiple comparisons). Data are presented as mean relative percent luciferase expression, mean ± S.E., with n = 2–3 replications per condition.

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