A 60-year-old woman being treated for hypertension suffered from severe headaches, abnormal behaviour, apathy, motor aphasia, bradykinesia and gait disturbance after mild abdominal pain and fever. There was a small norovirus outbreak in her area of the town and five of her family members living with her—her son, daughter-in-law and three grandchildren—got severe diarrhoea and slight fever. Two days before she visited our hospital, she felt tired and was bedridden with a fever of around 38.0 °C. When she got up at 02:00 and went to the bathroom, her husband mentioned that she kept opening a bathroom door contrary to her normal behaviour. She did not say anything when she was asked to answer easy questions. The only thing she could do to answer was nod. Her husband took her to their family doctor and she was transferred to our hospital. In the emergency room, her body temperature was 36.9°C, and neurological examinations revealed slight dull consciousness, motor aphasia, mild nuchal stiffness and bradykinesia without rigidity of her extremities or paresis. She was able to walk slowly without a stick at that time.
Initially, she was given a CT scan and MRI for suspected brain stroke; however, there was no abnormal finding apart from mild brain atrophy related to her age. Follow-up brain MRI study the next day showed slightly high signals in the cortex of the opercular part and insula at left frontal lobe on the fluid-attenuated inversion-recovery (FLAIR) images, but no abnormal signal in other images, such as diffusion-weighted imaging (DWI), T2WI, T1WI and enhanced T1WI (). EEG presented generalised slow waves as a background activity without any spike or slow wave complex. Her whole body CT scan and Gallium-67 scintigraphy detected no sign of tumour. Laboratory tests revealed normal red blood cell count (402×104/µl), a haemoglobin of 13.0 g/dl, normal white blood cell count (5300/µl) consisting 65.0% of neutrophils and normal platelet count (29×104/µl). Blood chemistry studies were also normal including electrolytes: creatine phosphokinase 123 U/l, alanine transaminase 17 U/l, aspartate aminotransferase 13 U/l, triglyceride (TG) 81 mg/dl, high-density lipoprotein cholesterol 39 mg/dl, total cholesterol (T-CHO) 194 mg/dl, HbA1c 5.5%, blood urea nitrogen (BUN) 11.7 mg/dl, creatinine 0.51 mg/dl, Na 137 mEq/l, Cl 100 mEq/l, K 4.1 mEq/l, Ca 8.5 mg/dl and C reactive protein (CRP) 0.16 mg/dl. Her thyroid hormone levels were within normal range: free-T4 1.08 ng/dl, free-T3 2.10 pg/ml, thyroid-stimulating hormone 2.00 μIU/ml. No autoimmune antibodies were detected: anti-thyroglobulin antibody 1.8 ng/ml, anti-thyroid peroxidase antibody <0.5 U/ml and she was negative for lupus erythematosus test and rheumatoid factor. There was no detectable antiviral IgG or IgM in her serum against echovirus type 3, 7, 11, 12; coxsackievirus A9, B1, 2, 3, 4, 5, 6; herpes simplex virus; varicella-zoster virus; cytomegalovirus; influenza virus; human T cell lymphotropic virus type 1; hepatitis C virus or hepatitis B virus. β-D glucan was negative: 8.3 pg/ml (<20). On the first hospital day, her cerebrospinal fluid (CSF) study showed normal cerebrospinal pressure (120 mm H2O) and her cell count was mildly increased (25/mm3) and all mononuclear cells. CSF protein level was 46.4 g/dl and CSF glucose level was 69 mg/dl. Day 2, her CSF was still watery clear tapped with normal pressure (65 mm H2O) and her cell count was still mildly increased (10/mm3) and all mononuclear cells. CSF protein level was clearly high at 130 mg/dl with lower IgG index (0.63) and CSF glucose level was normal at 59 mg/dl (blood glucose 77 mg/dl). Myelin basic protein level was also normal (31.8 pg/ml) and negative for oligo clonal IgG band. No pathogens that might cause meningitis were detected by PCR in her CSF samples: herpes simplex virus, Epstein–Barr virus, cytomegalovirus and tuberculosis genomic DNA. Cryptococcus antigen was also negative. She was still not able to talk with her family and she did not get well by feeding nutrition, drip infusion of glycerol (1000 ml/day) or and acyclovir (1500 mg/day). On day 3, we decided to treat her with drip infusion of methyl-prednisolone (1000 mg/day) and globulin (total 12.5 g) for 3 days (from day 3 to day 5). Soon after the first drip infusion she started to speak more than one phrase and ate jelly with a spoon by herself. She improved quickly over the days and she totally recovered without any further disability on day 10. After recovering, she was able to explain the situation during her severest days: she could understand what she was asked about, but could not say anything to answer.
Figure 1 MRI on day 2 of hospital admission shown. There is a slight high signal on the cortex of the opercular part and insula at left frontal lobe on the fluid-attenuated inversion-recovery (FLAIR) image, but no signal change on diffusion-weighted image (DWI), (more ...)
The RT-PCR (and nested RT-PCR) tests7
were done with specific primer pairs for norovirus, sapovirus, astrovirus, aichi-virus, adenovirus, rotavirus group A and C, enterovirus from her CSF and stool samples, following a usual protocol previously described.2
In her stool sample, we only detected group 2 of the norovirus RNA genome as well as in those of all six family members. However, even using a nested RT-PCR method, we could not detect any evidence of norovirus genome in her CSF tapped on the first and second hospital day. We clinically diagnosed this patient to be suffering from an adult NvREE on circumstantial evidence.