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BCL-2 family proteins play a central role in regulating clonal selection and survival of lymphocytes and are frequently over expressed in lymphomas. Navitoclax (ABT-263) is a targeted high-affinity small molecule that occupies the BH3 binding groove of BCL-2 and BCL-XL and inhibits their anti-apoptotic activity. Experimentally, navitoclax kills cells in a BAX/BAK-dependent manner and results in regression of lymphoid tumors in xenograft models.
This is a phase I dose-escalation study of navitoclax in patients with relapsed or refractory lymphoid malignancies. Study endpoints included safety, maximum tolerated dose (MTD), pharmacokinetic profile and clinical activity. In addition, mechanism-based pharmacodynamic effects on platelets and lymphocytes were assessed. Navitoclax was orally administered and assessed on an intermittent schedule of once daily for 14 days followed by 7 days off (14/21 days) or on a continuous once daily schedule (21/21 days). This trial is registered with ClinicalTrials.gov, number NCT00406809.
Fifty-five patients were enrolled, (median age 59 years, IQR 51–67), of whom two did not complete the first cycle and were not evaluable for assessment of dose-limiting toxicity (DLT). Common toxicities included grade 1/2 diarrhea and fatigue in 31 and 21 patients, respectively. Thrombocytopenia and neutropenia were the serious common toxicities with grade 3/4 observed in 29 and 17 patients, respectively. On the intermittent schedule (14/21), 5 DLT’s were observed; two due to hospitalizations for bronchitis and pleural effusion, and one each due to grade 3 transaminase elevation, grade 4 thrombocytopenia and grade 3 cardiac arrhythmia. Navitoclax caused a rapid and dose-dependent decline in peripheral platelets following initial drug exposure, followed by a rebound. To reduce the platelet nadir associated with intermittent dosing, a lead-in dose followed by continuous dosing (21/21 schedule) was examined. Three DLT’s were observed on this schedule (21/21); one each due to grade 4 thrombocytopenia, grade 3 transaminase elevation and grade 3 gastrointestinal bleed. Navitoclax showed a pharmacodynamic effect on circulating platelets and T-cells. Based on these findings, a 150 mg 7-day lead-in dose followed by 325 mg dose administered on a continuous (21/21) schedule was selected for phase II study. Clinical responses occurred at all dose levels and in multiple histologies. Partial responses were observed in 10 of 46 patients with evaluable disease, and the responders had a median progression-free survival of 455 days (IQR 40-218).
BCL-2 family proteins play a central role in lymphocyte biology where they regulate clonal selection and survival. (1–3) It is therefore not unexpected that pro-survival BCL-2 proteins are benefactors of upstream driver mutations or are themselves over expressed through translocation or amplification in many lymphoma subtypes.(4–7) The importance of these proteins in normal and malignant lymphoid biology has driven the search for inhibitors. An effective strategy to develop a highly specific inhibitor involves high-throughput NMR-based screening, parallel synthesis and structure-based design to identify small molecules that bind BCL-XL.(8, 9) This effort yielded ABT-737, which showed high affinity binding to BH3-only proteins with an affinity two to three orders of magnitude greater than previously reported compounds. Mechanistic studies showed that ABT-737 does not directly initiate apoptosis but enhances the effect of death signal and is synergistic with cytotoxic agents and radiation.(10) To overcome the low solubility and oral bioavailability of ABT-737, the ABT-263 analog (navitoclax) was developed for clinical investigation. Pre-clinical studies confirmed that like ABT-737, navitoclax had a high affinity for the anti-apoptotic BCL-2 family proteins and killed in a BAX/BAK-dependent manner.
Navitoclax demonstrated broad activity against a panel of human tumor cell lines including 11 of 23 hematological cell lines at an EC50 < 1 µmol/L.(10, 11) In vivo, navitoclax induced durable and complete tumor regressions in a murine xenograft model of acute lymphocytic leukemia and significantly improved the cure rate of rituximab plus chemotherapy in a xenograft model of mantle cell lymphoma.(11) We report the first in-human phase 1 and pharmacodynamic results of navitoclax, which induced durable responses in drug resistant lymphoid malignancies and mechanism specific pharmacodynamic adverse effects.
This phase 1 dose-escalation study utilized a modified Fibonacci 3+3 design to evaluate the safety, pharmacokinetics, pharmacodynamics, and preliminary efficacy of navitoclax in relapsed/refractory lymphoid malignancies. Eligibility included subjects with a histologically confirmed lymphoid malignancy as defined in the World Health Organization (WHO) classification; at least 1 prior chemotherapy regimen and relapsed or refractory disease; an Eastern Cooperative Oncology Group (ECOG) performance status 0–1; age ≥ 18 years; adequate bone marrow (platelets ≥ 100,000/µl; absolute neutrophil count ≥ 1000/µl; hemoglobin ≥ 9.0 g/dL); serum creatinine ≤ 2.0 mg/dL or calculated creatinine clearance ≥ 50; adequate hepatic function (AST and ALT ≤ 3.0 upper limit of normal (ULN); bilirubin ≤ 1.5 × ULN unless presence of Gilbert’s Syndrome); and adequate coagulation (PTT, and PT ≤ 1.2 × ULN). Initial evaluation included a history and physical examination, standard blood tests, whole body computed tomography (CT), and bone marrow biopsy. Tumor responses were evaluated by CT, bone marrow biopsy and peripheral lymphocyte counts after Cycle (C) 2 and C4, and every third cycle thereafter, and followed International Working Group criteria.(12, 13) Overall survival and progression-free survival were calculated by the Kaplan-Meier method.(14)
This study was Institutional Review Board approved, complied with the Declaration of Helsinki, and patients gave written informed consent. The study was conducted at seven sites and co-sponsored by Abbott Laboratories and Genentech, and All authors had access to the primary data and approved the manuscript.
Navitoclax was orally administered over multiple dose levels (Table 1). Two schedules were evaluated: (i) an intermittent schedule on days 1–14, every 21 days (14/21) and (ii) a continuous schedule (21/21) that was preceded by a lead-in dose of 150 mg for 7–14 days to reduce acute thrombocytopenia (Figure 1). Navitoclax was escalated in cohorts of 3–9 patients. Treatment was continued until tumor progression or unacceptable toxicity as defined below. The maximum tolerated dose (MTD) was defined as the dose level at which no more than one of six patients experienced dose-limiting toxicity (DLT). DLT was defined as grade 4 thrombocytopenia, and/or at least grade 2 bleeding. Other grade 3, 4, or 5 adverse events were considered dose-limiting except grade 3 or 4 neutropenia less than or equal to 7 days without fever, grade 3 or 4 lymphopenia, and grade 3 nausea, vomiting or diarrhea unless treatment unresponsive. Unexpected grade 2 toxicity that required dose modification or delay of ≥ 1 week was also dose limiting. Adverse events were graded using NCI CTCAE V3.
The dose of navitoclax was interrupted for any pre-dose platelet count < 25,000/µl and could be restarted at a reduced dose level if the platelets recovered to > 50,000//µl. Navitoclax was also interrupted for any clinically significant bleeding, defined as Grade 2 or higher hemorrhage and/or a DLT and could be restarted at a reduced dose level if the toxicities resolved. The investigator and study sponsor jointly determined the reduced dose level. Patients were removed from study if they underwent more than three dose reductions and had no objective response.
Blood samples for pharmacokinetics (PK) were collected fasting on C1, Day (D) –3 and non-fasting on D1 and 14 pre-infusion, and on C2–6 on Day 14 pre-infusion. A 24-hour urine collection was obtained after dosing on C1 D–3. Navitoclax levels were determined using a liquid chromatography method with Tandem Mass Spectrometric detection. PK parameters assessed included maximum concentration (Cmax) time to Cmax, (Tmax0), oral clearance (CL/F), and area under the concentration curve (AUC).
Descriptive statistics of medians and ranges were calculated for continuous parameters, as well as frequencies and percentages for categorical parameters. Time to event analyses used the method of Kaplan and Meier. Progression free survival (PFS) is defined as the number of days from the date a subject started study drug to the date the subject experiences an event of disease progression, or to the date of death if disease progression is not reached and the death occurred within 2 cycles of the date of last available tumor evaluation. Subjects were censored if they had not experienced disease progression or death at their last available tumor evaluation. For overall survival, subjects were censored on the last day known to be alive. Statistical Analyses Software (SAS) version 9.2 was used for all analyses except for determination of the P-values of the Sign-test on change in CD3+ from baseline, which used SAS version 8.2.
This trial was sponsored by Abbott Laboratories and Genentech. M06-814 was the first in human protocol submitted by Abbott with the initial IND for ABT-263. Although Abbott collaborated with advisors (including the authors) during the study design and subsequent amendments, the study was designed and data collected, analyzed and interpreted by the trial sponsor, with input from the authors and investigators in accordance with Good Clinical Practices. The initial draft provided by WHW, was reviewed and commented on by all authors and by employees of Abbott Laboratories and Genentech. WHW, the first and corresponding author, and Abbott authors HX, YLC, YC, TBB, SWE, SHR, APK, SEH and RAH had full access to the study raw data. Authors OAO, MSC, ASC, JFG, JPL, AT and KD were provided with the full data set upon request. WHW took full responsibility for the writing and final decision to submit this manuscript.
Fifty-five patients were enrolled and 53 were evaluable for DLT; two patients enrolled at the 275 mg dose level did not complete cycle one due to voluntary withdrawal and disease progression, respectively (Table 1). The patients had a median age of 59 years and most were heavily pretreated.
Two dose schedules were examined; daily for 14 days followed by a 7-day rest (14/21) and continuous dosing as described earlier (21/21). Dose cohorts ranged from 10–440 mg orally per day for the 14/21 schedule. Overall, five DLT’s were observed; one at 160 mg and two each at 315 and 440 mg dose levels. Two DLT’s due to hospitalizations for bronchitis and pleural effusion were judged unrelated to navitoclax, and one each due to grade 3 transaminase elevations, grade 4 thrombocytopenia and grade 3 atrial fibrillation were judged possibly or probably related to drug. Based on the finding that the patient with the cardiac arrhythmia had a prior history of atrial fibrillation and no other significant cardiac events were observed on this study (Table 2), it appears unlikely that navitoclax causes significant cardiac toxicity. Based on the occurrence of only one DLT in the first 6 patients treated at the 315 mg cohort, and two DLT’s in the 440 mg cohort, 315 mg was identified as the safe tolerated dose for the intermittent schedule.
On the intermittent schedule, significant platelet nadirs occurred with the initial doses of each cycle, followed by a modest rebound. To help reduce the acute platelet nadirs and grade 4 thormbocytopenia, a lead-in dose followed by continuous dosing (21/21 schedule) was examined in dose cohorts of 200–425 mg. Three DLT’s were observed, one at 275 mg and two at 425 mg dose levels; grade 4 thrombocytopenia, grade 3 transaminase elevation and grade 3 gastrointestinal bleed, respectively, all possibly or probably related to navitoclax. Based on these findings, a 150 mg 7-day lead-in dose followed by 325 mg dose administered on a continuous (21/21) schedule was selected for the phase II study.
When all dose levels are considered, the most common toxicities were grade 1/2 gastrointestinal complaints likely due to the drug vehicle (Table 2). Nausea and vomiting were managed with anti-emetics and diarrhea was managed with diphenoxylate and atropine (lomotil). Fatigue was relatively common but not dose limiting. Eight patients developed respiratory infections and/or bronchitis (Table 2), none of which were associated with grade 4 neutropenia. Thrombocytopenia and neutropenia were the serious common toxicities with grade 3 or 4 thrombocytopenia or neutropenia observed in 29 and 17 patients, respectively (Table 2). Unlike the thrombocytopenia, neutropenia was not clearly associated with dose level and tended to occur on the later cycles; it was also reversible upon navitoclax discontinuation. Grade 4 thrombocytopenia and neutropenia were managed by temporary suspension and dose reduction of navitoclax and filgrastim was used for persistent grade 4 neutropenia. These results indicate that navitoclax may cause unacceptable hematological toxicity in patients with limited bone marrow reserve. Overall dose levels, 11 patients required at least one dose reduction and 6 patients withdrew their consent for treatment, two of which were due to toxicity.
Navitoclax PK was assessed on the 14/21-day schedule (Figure 2 and Table 3). To assess food effects on absorption, navitoclax was administered fasting on day –3 and non-fasting on days 1–14 of cycle one. Overall, food increased the oral bioavailability by approximately 20% with the current lipid formulation. Exposure was dose-proportional between 10 mg and 440 mg with approximately 40% interpatient variability in the plasma AUC. Navitoclax achieved peak concentrations (Cmax) at approximately 9 hours post-dose with a half-life of approximately 17 hours, and first order elimination kinetics. The projected effective exposure of 55–88 µg·hr/mL based on animal models was achieved at dose levels of at least 315 mg/day in patients. Navitoclax could not be detected in urine, indicating negligible renal elimination.
We hypothesized that if navitoclax functionally inhibited BCL-2 and BCL-XL, it should decrease the survival of normal cells in which these proteins regulate survival such as in T-cells and platelets, respectively.(15, 16) To assess this, we measured the absolute number of CD3+ cells in the circulation before, on day 14 of cycle 1, and at the end of treatment (Figure 3A). Patients showed a relatively rapid decrease in T-cells after only 14 days of drug exposure (median reduction 241 CD3+ cells/µl), which did not worsen with further treatment (Figure 3A). Importantly, the loss of T-cells was modest and not associated with opportunistic infections.
We also examined the kinetics of circulating platelets during navitoclax exposure. There was a significant reduction following a single dose of navitoclax, which was observed to begin as soon as one hour after dosing (data not shown) (Figure 3B). With continued dosing, there was a modest rise from nadir levels. To obviate the acute nadirs associated with intermittent dosing (14/21), we tested a lead-in dose followed by continuous dosing (21/21) and observed higher nadirs. Consistent with a pharmacodynamic effect, the severity of the platelet nadirs was concentration dependent (Figure 3C) and related to dose level (data not shown).
Overall, 21 of 46 patients with evaluable adenopathy showed some tumor reduction. Furthermore, 10 of these patients achieved a partial response (PR) lasting a median (range) of 455 days (IQR 40–218) (Figure 4). Responses occurred across dose levels and tumor types and were observed after a median (range) of 3.5 (2–10) cycles. Chronic lymphocytic leukemia/lymphoma (CLL/SLL), a disease of B-cell accumulation, showed the greatest sensitivity. Among the seven patients with CLL, all achieved at least a 50% reduction in their leukemia cells, and 8 of 16 patients with measurable adenopathy achieved a PR, which included patients with bulky adenopathy. Including all 20 patients with CLL/SLL, the median (range) PFS was 246 days (IQR 49-309) and the median overall survival has not been reached. Measurable tumor reduction was also observed in 6 of 16 patients with follicular lymphoma. Overall, the 16 patients with follicular lymphoma had a median PFS of 88 days (IQR 42-92). A PR was achieved in a single patient with an NK/T-cell lymphoma. The patients with CLL/SLL or follicular lymphomas had received a similar number of prior regimens with a median (range) of 4 (1–12) and 5 (1–11), respectively.
Fifty-three evaluable patients received navitoclax on two different treatment schedules. Navitoclax demonstrated a high therapeutic index with a low incidence of off-target toxicities. The major off-target toxicity was gastrointestinal, which appears likely related to the phosphatidylcholine solubilizer. Though uncommon, transaminase elevation at higher dose levels and neutropenia after prolonged drug exposure were also observed. Navitoclax also demonstrated favorable pharmacokinetic properties and therapeutic index. Due to good oral absorption, exposure was dose proportional, and the approximate 17-hour half-life allowed daily dosing. Furthermore, concentrations shown to be effective in preclinical models were achieved at the recommended phase 2 dose of 325 g/day.
The pharmacodynamic effects of navitoclax on circulating lymphocytes and platelets are novel and consistent with on-target mechanisms. Based on preclinical evidence that platelet senescence entails an apoptosis-like process mediated through BCL-XL, it is likely that intravascular apoptosis is responsible for the acute thrombocytopenia following navitoclax.(17, 18) Furthermore, the relative resistance of younger platelets to navitoclax appears to be due to their higher levels of BCL-XL, which explains the platelet kinetics observed in patients and in pre-clinical animal models using ABT-737.(18). It is also likely that navitoclax induces apoptosis of normal lymphocytes through its inhibitory effect on BCL-2. These results suggest that the pharmacodynamic effects of navitoclax are biomarkers of pharmacological inhibition of BCL-2 and BCL-XL, and should be observed with all effective inhibitors. We also observed grade 3 or 4 neutropenia in 17 patients, which raises the question of whether navitoclax may also have a pharmacodynamic effect on myeloid cells. To help assess this, we performed preliminary methylcellulose-based in vitro colony forming assays to assess the effects of ABT-737, a navitoclax analog, on human hematopoietic progenitors. In the presence of multilineage cytokines, ABT-737 inhibited the growth of both erythroid and common myeloid precursors (IC50 of 1.0 µM and 0.9 µM, respectively) (data not shown). Furthermore, BCL-2 has been shown to be necessary for the in vitro survival of myeloid progenitors under conditions of cytokine withdrawal, suggesting the neutropenia we observed in our study could be due to inhibition of BCL-2 by navitoclax.(19)
Navitoclax demonstrated clinical activity at all dose levels and across tumor types with the greatest activity seen in CLL. Letai et al. recently proposed a model, termed BH3 profiling, which may explain the differential sensitivity of lymphoma cells to BCL-2 inhibition.(20, 21) They proposed that sequestration of the activator BH3 only proteins, BIM or BID, by BCL-2 produces a “primed” state in which BCL-2 inhibition releases activator proteins and induces apoptosis. Using BH3 profiling, the group found a pattern of BCL-2 dependence in CLL with high BIM:BCL-2 complex levels and exquisite sensitivity to ABT-737.(20) Interestingly, we observed few bone marrow responses with navitoclax, even among patients with robust nodal and blood responses, which may be due to the influence of the microenvironment on increased expression of MCL-1, BCL-XL or BCL-2A1.(22) Though the activity of navitoclax was less apparent in other lymphoma subtypes, it has synergistic activity with chemotherapeutic agents in preclinical models. Given the complexity of the primed BCL-2 phenotype, and the influence of the microenvironment and upstream pathways, we hypothesize that the greatest benefit of navitoclax will be observed in combination with other agents.
Several other inhibitors of BCL-2 have undergone clinical testing. Two of these, obatoclax and gossypol, are small molecules that are reported to be pan-BCL-2 inhibitors.(23, 24) Thus far, they have shown little to no clinical activity. Furthermore, they have been shown to kill cells in a BAX/BAK-independent manner, challenging the functional significance of their weak affinity for BCL-2 family proteins and their true mechanisms of action.(25–27) A review of their toxicities also showed no on-target pharmacodynamic effects on platelets, suggesting they do not achieve effective inhibition of BCL-XL.(23, 25–28) Gossypol primarily caused gastrointestinal side effects, which were dose limiting, but caused no significant laboratory abnormalities.(23) While navitoclax also had gastrointestinal side effects, they were low grade and likely due to the drug solubilizer. Infusion-related somnolence and neurologic symptoms were the most common toxicities associated with obatoclax, and were dose limiting.(24) Modest hematological effects were also observed, including mild thrombocytopenia, which were attributed to progression of the patients’ underlying CLL.(24) A third drug, oblimersen, is an antisense oligodeoxyribonucleotide that down regulates BCL-2 translation.(29) A phase I study demonstrated limited single agent activity with only one response in 21 patients.(30) Interestingly, like navitoclax, oblimersen caused thrombocytopenia, which progressively worsened during the drug infusion and correlated with its plasma concentration.(30) While these results indicate that oblimersen has a pharmacodynamic effect on platelets, its indirect effect on BCL-2 levels through inhibition of BCL-2 mRNA is inconsistent with a direct inhibition of BCL-XL. In contrast to these other putative BCL-2 inhibitors, induction of apoptosis by navitoclax in vitro can be attributed to inhibition of BCL-2 family proteins. Coimmunoprecipitation studies show that navitoclax induced a dose-dependent decrease in BIM:BCL-2 family protein interactions in BCL-XL and BCL-2 over expressing prolymphocytic murine cell lines. Navitoclax also induced a dose-dependent decrease in cytosolic BAX and an increase in cytochrome c within 2 hours of treatment of a BCL-2 dependent human small cell lung cancer (SCLC) cell line.(11, 27)
The importance of BCL-2 family proteins in lymphoid biology and pathogenesis has driven the search for small molecule inhibitors of this pathway. With few exceptions, lymphomas express increased BCL-2, which may be physiological or pathogenetic. In follicular lymphoma and the germinal center subset of diffuse large B-cell lymphoma (DLBCL), translocation of the BCL-2 locus and immunoglobulin heavy chain promoter (t(14;18)) drives BCL-2 production, whereas in the post-germinal center subset of DLBCL, BCL-2 may be over expressed through amplification or transcriptional activation.(6, 31–33) CLL employs yet another mechanism whereby deletion or down regulation of miRNA miR-16-1 and miR-15a drives post-transcriptional increases in BCL-2.(34, 35) The occurrence of such varied pathogenetic mechanisms that increase BCL-2 expression points to the evolutionary significance of this pathway in lymphomagenesis, and the potential importance of this target in lymphoid malignancies.
Our study provides the first clinical insights to our knowledge into a pharmacologically active BCL-2 family inhibitor. We are currently investigating navitoclax in an expanded cohort of indolent and aggressive B-cell lymphomas. There are ongoing phase I studies of navitoclax with other agents including rituximab (CD20 monoclonal antibody), bendamustine and rituximab, and fludarabine, cyclophosphamide and rituximab combinations in lymphoma and CLL. Furthermore, to overcome the pharmacodynamic effect of navitoclax on circulating platelets, which will limit its ability to be combined with cytotoxic agents, a selective inhibitor of BCL-2 is under development.(36)
There exists an extensive literature that demonstrates apoptosis, or programmed cell death is the principal mechanism through which unwanted or damaged cells are safely eliminated.(37–40) Although cancer has historically been considered a disease of uncontrolled cell division, abnormal resistance to apoptosis is now understood to contribute to tumor initiation, progression, and resistance to chemotherapy. Defects in the apoptotic pathway confer a survival advantage that allows a net increase in tumor cell number and the accumulation of oncogenic mutations, which gives rise to highly aggressive tumors. Interactions between pro-apoptotic (pro-death) and antiapoptotic (pro-survival) BCL-2 family proteins regulate the initiation of the intrinsic apoptosis pathway. The pro-death proteins of BAX and BAK are direct mediators of apoptosis and are absolutely required for the initiation of the mitochondrial apoptosis pathway.(41) Over expression of anti-apoptotic BCL-2 family proteins (BCL-XL, BCL-2, BCL-W, A1, MCL-1) suppresses BAX and BAK and prevents the initiation of the apoptosis, thereby protecting cancer cells from responding to proapoptotic signals.
Compelling evidence for the role of BCL-2 family proteins in lymphoid biology and pathogenesis has driven the search for inhibitors.(42) With infrequent exception, lymphomas express BCL-2, which may be physiological and/or pathogenetic. The search for BCL-2 inhibitors has primarily relied on cytotoxicity screening. While such methodologies have lead to the identification of small molecules with low affinity inhibition and/or off target effects, these agents have shown relatively little single agent activity.(23, 26) An alternative strategy employed in the development of navitoclax entailed a structure-based design to identify small molecules that bind BCL-XL, which lead to the high affinity inhibitor navitoclax.(8, 10)
In the present study, we report that navitoclax, a high affinity inhibitor of BCL-2 family proteins, has clinical activity in lymphoid malignancies and has on-target pharmacodynamic effects on platelets and T-cells, where BCL-XL and BCL-2 regulate survival. While other putative BCL-2 inhibitors have undergone clinical testing, they have not shown significant clinical activity or targeted pharmacodynamic effects, which likely reflects low inhibition of BCL-2 family proteins. Thus, the present study provides the first proof of concept in humans, to our knowledge, that inhibition of BCL-2 family proteins leads to tumor cell death and targeted cell death of platelets and T-cells. As most cytotoxic agents induce apoptosis as a primary mechanism of cell kill, modulation of the apoptotic “threshold” with agents such as navitoclax is hypothesized to significantly increase the efficacy of current cytotoxic treatments. Presently, phase I trials are underway to assess the safety of navitoclax with cytotoxic agents. Further studies will be necessary to determine if navitoclax is safe and effective before it can be used in standard treatment.
The authors would like to thank Juliann M. Dziubinski, Katherine Papp, Lori Gressick, Michael D. Dawson and Renee Greco, for operational support; Di Li, and Joseph E. Beason for statistical analyses; Christin Tse, Morey L. Smith, Stephen K. Tahir, Kennan C. Marsh, Joy L. Bauch, Sherry J. Morgan, Joel Leverson, and Anne H. Illi-Love for their knowledge of the preclinical data referenced and their contributions during manuscript preparation, and Ai Q. Lockard for editorial assistance for the manuscript. The authors would also like to thank the contributions of the research data managers, coordinators and nurses including Margaret Shovlin, Barbara MacGregor Cortelli, Ameet Narwal, Barbara Anderson, Alice Mohr, Hazel Reynolds, Susan Twohig, Jennifer Pappanicholaou, Payal Dixit, June Greenberg, and Nancy Berman. This study was funded by Abbott Laboratories and Genentech, Inc.
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Author ContributionsWyndham H. Wilson, Owen A. O’Connor, Myron S. Czucman, Ann S. La Casce, John F. Gerecitano, John P. Leonard, Anil Tulpule and Kieron Dunleavy were responsible for patient enrollment and collection and assembly of data. Wyndham H. Wilson, Sari H. Enschede, Andrew P. Krivoshik, Rod A. Humerickhouse, Hao Xiong, Yi-Lin Chiu, Yue Cui, Todd B. Busman, Steven W. Elmore, and Saul H. Rosenberg were responsible for data analysis and interpretation. All authors were responsible for writing, editing and final approval of the manuscript.
Conflict of Interest
WHW received funds from Abbott Laboratories to support his travel to one protocol meeting for this study. MSC receives grant and consulting fees and honorarium from Abbott Laboratories. HX, YLC, YC, TB, SWE, SHR, APK, SHE and RAH are employee of Abbott Laboratories; HX, YLC, TB, SWE, APK, SHE and RAH have stock in the company, and APK holds patents assigned to and receives funds for travel, accommodation and meeting expenses from Abbott Laboratories. JFG, JPL, KD, have no conflicts to declare. OAO, ASL and AT have yet to provide their conflict of interest and financial disclosures.