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Journal of Community Health
J Community Health. 2011 February; 36(1): 42–46.
Published online 2010 June 8. doi:  10.1007/s10900-010-9283-0
PMCID: PMC3020291

Prevalence of Hepatitis B Virus (HBV) Infection Among Hmong Immigrants in the San Joaquin Valley


Chronic hepatitis B infection (HBV) is the major cause of primary liver cancer worldwide and Asians are disproportionately affected. The prevalence of HBV among most Asian American groups has been well documented, except in Hmong immigrants in the United States. The aim of this study was to determine the prevalence of HBV among Hmong immigrants in the San Joaquin Valley of California. A convenient sample of 534 Hmong age ≥18 years was recruited at various locations throughout Fresno County. Blood samples from study participants were collected and tested for hepatitis B surface antigen (HBsAg) by enzyme-immunoassay. Two hundred and eighty-nine females and 245 males of Hmong descent (mean age, 43.93) were screened. Eighty-nine (41 males and 48 females) were positive for HBsAg, which accounts for a prevalence of 16.7% (95% C.I. 13.5–19.9). The majorities of HBsAg positive patients were ≥40 years (64.2%), married (66.7%), born in Laos (87.3%), and had lived in the United States ≥20 years (62.5%). Only 37.5% of the participants reported having a primary care physician. Our study revealed that approximately one out of every six Hmong immigrants screened was infected with HBV. Based on our findings, more than one-third of these infected patients have no primary care physician to provide further treatment, surveillance for liver cancer, or vaccination of their families. This supports the Institute of Medicine’s recent recommendations to the Center for Disease Control to engage in a national Hepatitis B surveillance system.

Keywords: Hepatitis B, Hmong, Hepatocellular cancer


HBV is endemic in the Asia–Pacific region. Many studies have suggested that this is due to vertical transmission from mother to newborn coupled with low vaccination rates [1, 2]. Approximately 90% of infants and 25–50% of children aged 1–5 years will remain chronically infected with HBV after exposure [3]. Twenty-five percent of those who become chronically infected during childhood and 15% of those who become chronically infected after childhood die prematurely from cirrhosis or liver cancer [4, 5]. In the United States, there are an estimated 1.25 million people with chronic HBV infection [6, 7]. The prevalence of chronic HBV infection in Asian Americans is between 10 and 15% compared to only 0.3% for the general United States population [8]. Chronic HBV infection is a major cause of hepatocellular carcinoma (HCC). A recent Taiwanese study reported 94.9% infection rate with HBV at the time of HCC diagnosis [9]. HCC is the leading cause of death among Asian Americans between the ages of 25–65 [1012]. In the United States, chronic HBV infection results in estimated 2,000–4,000 deaths per year [3].

Approximately 12 million Asian Americans currently reside in the United States; of these, 5–10% of first-generation immigrants may be chronically infected with HBV. Thus, the true prevalence of chronic HBV infection is probably in excess of 2 million. On the basis of immigration patterns in the last decade, it is estimated that every year 40,000–45,000 people from HBV-endemic countries legally immigrate to the United States. In addition, approximately 45,000 new HBV infections occur each year. The prevalence of HBsAg among Asian refugees entering the United States between 2006 and 2008 has been reported as 4.8% [13]. In 2001, the lead author of our study participated in a prospective survey of Vietnamese immigrants in Orange County, California and found that 12.9% were HBsAg positive [14].

Several studies have demonstrated the prevalence of HBV among other Asian groups in the United States, however, there are no prospective data available for the Hmong population. A retrospective study conducted in St. Paul, Minnesota found that Hmong Americans have an 18% prevalence of HBV, with the rate of infection being highest among patients who were between 15 and 19 years of age [15]. Another retrospective study from Michigan reported that Hmong Americans had the highest prevalence rate of HBV infection among those screened [16].

In the mid 1970s after the Vietnam War, large numbers of Hmong began arriving in the United States as refugees from Laos. According to the United States Census bureau in 2000, there were approximately 186,310 Hmong in the United States. The San Joaquin Valley in California is one of the fastest growing Hmong communities with a population estimated at 65,095 [17]. There are limited data regarding the health status of the Hmong in central valley especially morbidity and mortality associated with cancer [18, 19]. To date, there is no prospective HBV screening study in the Hmong population. Therefore, the prevalence of HBV among Hmong in San Joaquin Valley remains unknown. Such screening is essential to educate this population about the risks associated with HBV and optimize strategies for early detection and prevention of HCC [2022]. The objective of this HBV screening study was to determine the prevalence of HBV among Hmong population residing in Fresno County.


Sampling and Recruitment

Serological screening was provided to 534 Hmong age ≥18 years between December 2006 and December 2008. Recruiting efforts took place at many popular Hmong locations in the Fresno area. Local Hmong were made aware of the screening through several means including newspaper, frequent radio announcements, and flyers in Hmong. Educational slide presentations were delivered at Hmong church gatherings prior to all screening sessions. Two consecutive years at the International Hmong New Year Celebration in Fresno, stations for screening were established and educational material was provided. Blood samples were collected, coded, centrifuged on-site, and serum was tested for HBsAg by enzyme-immunoassays at a local laboratory.

After signing an informed consent, the study participants were given a choice of either a Hmong or English demographic and medical history questionnaire to complete. Hmong research volunteers assisted the participants in completing the questionnaire. All participants were notified of their blood test result through a confidential letter. Those who were found positive for HBsAg were invited for counseling. They were educated on the modes of transmission, complications of infection, prevention strategies and treatment options of HBV. The University of California, San Francisco—Fresno Institutional Review Board approved all study procedures.

Data Analysis

The presence of HBsAg in serum was determined by enzyme-immunoassays. Descriptive statistics were calculated and reported for demographic variables such as: age, sex, birthplace, years lived in US, marital status, vaccination, and establishment of care with a primary care provider. Ninety-five percent confidence intervals (CIs) were calculated for each variable.


A total of 534 Hmong were screened for HBV infection. Of these 45.9% (n = 245) were males and 54.1% (n = 289) were females. The mean age was 43.94 (SD = 15.62), the range being 18–90 years. The majority of the participants (88.1%, n = 457) were born outside of the United States, mainly in Laos (80.9%). Only 37.5% (n = 200) of the participants indicated that they had a primary care physician. The duration of residence in the United States was categorized into 10-year intervals, and the majority (63.6%) had lived in the United States for about 20–29 years. These characteristics are summarized in Table 1.

Table 1
Prevalence of hepatitis B virus (HBV) infection among screened participants by selected demographic characteristics

Of 534 participants tested for HBsAg, 89 (41 males and 48 females) were tested positive for HBsAg with a prevalence of 16.7% (95% CI 13.5–19.9). Among these subjects, the mean age was 45.22 years (SD = 17.35) with a range of 18–89 years. Nearly 92% (n = 79) were born outside the United States, of which 87.3% were born in Laos. The majority were married (67.7%, n = 56) and 53.1% (n = 17) had been living in the United States for about 20–29 years. Among these infected subjects, only 37.1% (n = 33) reported having a primary care physician (Table 1).


Our study found that 16.7% of Hmong in the San Joaquin Valley might be chronically infected with HBV. We did not evaluate the mode of transmission of HBV among the study participants. However, the literature suggests that the majority of Asians contract the virus during birth or in their early childhood [1, 2]. Our study showed that nearly 92% (n = 79) of HBsAg positive patients were born outside the United States. It is most likely that this Hmong population was already infected at the time of immigration to the United States. Our study found that nearly 75% (n = 63) of those who tested positive for HBV infection were married or previously married. Engaging in high risk behavior, such as having multiple sex partners, tattooing, and injecting drugs are not common practices in this community. A similar observation was made among Hmong in Thailand in an earlier study [23]. The majority of the participants were unaware of any known liver disease. These findings are compatible with previously published data in Asian populations [2428].

Rein et al. [13] published a recent study reporting the prevalence of HBsAg among refugees entering the United States during 2006–2008. They report a prevalence of 6.5% in Eastern Asians and 10.5% in Southeast Asians. The prevalence in those from Vietnam and Laos were 3.2 and 2.3%, respectively. However, this study was limited due to lack of information about the age and sex of the refugees. In comparison to CDC data from 1991 [29], the prevalence in Hmong refugees decreased from 15.5 to 2.3% in 2008. This 1991 prevalence for Laos was reported for the Hmong and for other Laotians without a single population-wide estimate. The decline in prevalence may be attributed to many factors, such as increased vaccination. The prevalence of 15.5% reported in 1991 is more consistent with the prevalence reported in our study because the majority (53.1%) of the Hmong we screened had lived in the United States for 20–29 years.

There are several limitations to our study. First, the free HBV screening sites were all located in Fresno County. Therefore, our findings may not be generalized to the Hmong population in other parts of the United States, or even to the entire Hmong population in the Central Valley due to our sampling frame. Second, the reluctance of this population to participate in screening may have affected the true prevalence. Many Hmong were afraid of getting their blood drawn and were apprehensive of the results. They were also worried about their family members knowing their health status. In the Hmong and other Asian American communities, hepatitis B may be stigmatized with having sexual partners, which can lead to ostracism. These observations are consistent with findings from other Asian American screening studies [2428]. Therefore, the prevalence reported in this study may be an underestimate of the true prevalence. Nearly 62.4% of the 534 screened Hmong indicated that they were not vaccinated or were unsure of their vaccination status. The majority of these patients had no primary care physician to provide further treatment, screening for liver cancer, or offer vaccination and preventive measures to curtail the transmission of HBV to their families.

The viral hepatitis surveillance system in the United States has been highly fragmented and poorly developed. There is a paucity of knowledge and awareness about chronic viral hepatitis on the part of health care and social service providers, at risk populations, members of the public, and policy makers. There is insufficient understanding about the extent and seriousness of this public health problem and inadequate public resources have been allocated to prevention, control, and surveillance programs. Due to inaccurate estimates of the burden of disease in the United States, policy-makers and the state governments could not allocate sufficient resources to viral hepatitis prevention and control programs. Between 2007 and 2008, the California Department of Health provided vaccination against hepatitis B to nearly 30,000 individuals. However, Asians Americans comprised only 7% of the vaccinated population. STD clinics made up 63% of the setting in which vaccination occurred, with community medical centers only accounting for 7% [30].

The Centers for Disease Control and Prevention has been recently recommended by the Institute of Medicine to conduct a comprehensive evaluation of the national viral hepatitis public health surveillance system. As per these recommendations [31] there is a need to develop specific cooperative viral hepatitis agreements with all state and county health departments to support core surveillance for viral hepatitis. This cooperative effort would support and conduct targeted active surveillance, including serologic testing, to monitor incidence and prevalence of viral hepatitis in populations not fully captured by core surveillance. The collaboration would allow key stakeholders to develop innovative and effective educational outreach programs to at risk individuals while increasing awareness in the general population.

Hepatitis B infection and its complications such as cirrhosis and HCC are largely preventable. We hope that adherence to the Institute of Medicine’s recommendations will provide an opportunity to decrease the impact of this disease in Asian Americans as well as the entire global community.


We would like to thank several volunteers who served as interpreters and phlebotomists for this study and helped the investigators to make this difficult screening project a success.

Open Access This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.


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