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BMC Biotechnol. 2010; 10: 90.
Published online 2010 December 21. doi:  10.1186/1472-6750-10-90
PMCID: PMC3018389

Analysis of conditional gene deletion using probe based Real-Time PCR

Abstract

Following publication of this article [1] the authors noticed that an incorrect probe reference was cited on page 3, 4, 5 and 6 ("UP #69, Roche Applied Science"). The correct probe that was used for the 1lox/2lox allele ratio analysis in the paper is as follows

Probe for 1lox/2lox allele quantification:

5'-6-FAM-atAaCtTCgtatagCATaCattatac-BHQ-1 -3'

(uppercase letters = LNA bases)

Manufacturer: EUROGENTEC, Seraing, Belgium

All other information and reaction conditions in the paper are correct as stated.

References

  • Weis B, Schmidt J, Lyko F, Linhart HG. Analysis of conditional gene deletion using probe based Real-Time PCR. BMC Biotechnology. 2010;10:75. doi: 10.1186/1472-6750-10-75. [PMC free article] [PubMed] [Cross Ref]

Articles from BMC Biotechnology are provided here courtesy of BioMed Central