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Terpenoids are among the most important constituents of grape flavour and wine bouquet, and serve as useful metabolite markers in viticulture and enology. Based on the initial 8-fold sequencing of a nearly homozygous Pinot noir inbred line, 89 putative terpenoid synthase genes (VvTPS) were predicted by in silico analysis of the grapevine (Vitis vinifera) genome assembly . The finding of this very large VvTPS family, combined with the importance of terpenoid metabolism for the organoleptic properties of grapevine berries and finished wines, prompted a detailed examination of this gene family at the genomic level as well as an investigation into VvTPS biochemical functions.
We present findings from the analysis of the up-dated 12-fold sequencing and assembly of the grapevine genome that place the number of predicted VvTPS genes at 69 putatively functional VvTPS, 20 partial VvTPS, and 63 VvTPS probable pseudogenes. Gene discovery and annotation included information about gene architecture and chromosomal location. A dense cluster of 45 VvTPS is localized on chromosome 18. Extensive FLcDNA cloning, gene synthesis, and protein expression enabled functional characterization of 39 VvTPS; this is the largest number of functionally characterized TPS for any species reported to date. Of these enzymes, 23 have unique functions and/or phylogenetic locations within the plant TPS gene family. Phylogenetic analyses of the TPS gene family showed that while most VvTPS form species-specific gene clusters, there are several examples of gene orthology with TPS of other plant species, representing perhaps more ancient VvTPS, which have maintained functions independent of speciation.
The highly expanded VvTPS gene family underpins the prominence of terpenoid metabolism in grapevine. We provide a detailed experimental functional annotation of 39 members of this important gene family in grapevine and comprehensive information about gene structure and phylogeny for the entire currently known VvTPS gene family.
Terpenoids are a large class of metabolites that are involved in the fragrance and aroma constituents of flowers and fruits, plant defense, and primary plant metabolism [2-4]. Although all terpenoids arise from a few structurally simple prenyldiphosphate precursors, an enormous assortment of thousands of possible molecules comes to fruition. This chemical diversity of terpenoid structures is attributed, in large part, to the myriad ways of folding and the eventual quenching of reactive carbocation intermediates in the reaction catalyzed by terpenoid synthases (TPS) [5,6]. The products of TPS can be further modified by other enzymes such a cytochrome P450 dependent monooxygenases and various transferases.
The initial 8-fold sequencing and assembly of a grapevine (Vitis vinifera L.) inbred Pinot noir genome (PN40024) lead to the prediction of 89 grapevine TPS (VvTPS) genes, which mirrors a vibrant role for terpenoid secondary metabolism in grapevine biology [7,8]. For example, in wine made from aromatic grape varieties, monoterpene alcohols such as linalool, geraniol, and cis-rose oxide impart important floral flavour qualities . Sesquiterpenes have also been identified as important indicators of grape aroma. Recently, Parker et al.  identified α-ylangene as a sesquiterpene metabolite marker associated with peppery aroma and taste in Australian Shiraz grape berries, and the sesquiterpene ketone, rotundone, was found to be the compound responsible for this attribute in peppery/spicy Australian Shiraz grapes and wines . Monoterpenes such as 1,8-cineol, and sesquiterpenes such as α-humulene, β-caryophyllene as wells as α- and γ-muurolene have been described in Cabernet Sauvignon pre-veraison berries . Similar profiles of terpene volatiles have also been documented from the headspace above Chardonnay leaves, flowers, and green berries . The presence of some terpenes early in berry development and in other parts of the plant may indicate a role in defense. Terpenoid volatiles are released from grapevines following insect feeding  or the application of methyl jasmonate .
Of all plant species for which genome sequences are available, the TPS gene family has been comprehensively explored only in Arabidopsis thaliana, in which 32 intact AtTPS genes were identified ; functions have been established for several of these genes [17-20]. A number of TPS genes have also been characterized against the background of the sequenced rice (Oryza sativa) genome [21-26] which has at least 40 TPS-like sequences identified (S. Aubourg unpublished results). For comparison, genome sequence analysis of poplar (Populus trichocarpa) identified 47 TPS genes , only two of which have been functionally characterized [28,29]. Prior to the sequencing of a grapevine genome, we reported on the cDNA cloning and product profiles of three VvTPS [30,31] and we detailed the involvement of valencene synthase, a sesquiterpene synthase, in the evolution of grapevine floral scent , but a comprehensive analysis of VvTPS has not been reported thus far.
The importance of terpenoids in grapevine biology and wine flavour and quality motivated a genome-wide inventory and functional characterization of the VvTPS gene family. We present the manually curated annotation of the VvTPS gene family from the current 12-fold genome sequence coverage. This work defines 69 putatively functional VvTPS, 20 partial VvTPS, and 63 probable VvTPS pseudogenes including VvTPS gene architecture and chromosome localizations. The VvTPS gene family shows extensive gene duplication and in many instances, functional diversification across all subfamilies except those involved in primary metabolism (subfamilies TPS-c and TPS-e). Conclusions regarding diversification are supported by phylogenetic analyses of the VvTPS family and functional characterization of heterologously expressed VvTPS proteins.
Screening of the predicted proteome and the six-frames-translated 12-fold genome sequence of V. vinifera with protein sequences of previously characterized TPS identified 152 loci exhibiting significant similarities with known TPS (see the Methods section for details). Our annotation of the 152 TPS-like gene models (Additional File 1) classified them into four types: (i) 53 are complete VvTPS genes that contain the expected functional motifs and domains [16,33,34] required to render them functional; (ii) 16 are complete VvTPS genes but the ORFs contain a frameshift or premature stop codon either due to a point mutation or a possible sequencing error; (iii) 20 are partial TPS genes disrupted by sequence gaps or located in scaffold extremities; and (iv) 63 are obvious pseudogenes disrupted by numerous deletions, frameshifts and/or stop codons (Additional File 1). After removing the genes of this last type, the number of potentially functional VvTPS ranges from a minimum of 53 up to 89 genes. The missing sequences of the partial genes (group iii) prevented meaningful sequence alignments and gene classification; therefore, we removed them from our further analysis which focused on the 69 VvTPS genes of groups (i) and (ii). The presence of cognate EST and/or cDNA sequences provides proof of transcription for 40 (58%) of them (Additional File 1).
The relatively high gene sequence and structure conservation across the plant TPS family  allow us to be confident in the result of the genome-wide VvTPS gene prediction, combining automatic and manual annotations. Manual curation and evaluation have substantially improved the identification of VvTPS genes: For example, out of the 69 VvTPS genes 12 were missed by the automated pipeline used for annotation of the grapevine genome [1,35]. Furthermore, intron-exon structures of 40 VvTPS genes required manual correction to obtain complete and consistent coding sequences. The results of the VvTPS gene annotations confirmed a large VvTPS gene family previously predicted from the 8-fold genome assembly  and expand the previous estimation of the VvTPS family size. While a lower estimate of only 35 VvTPS genes was reported from the analysis of a second heterozygous Pinot noir genome sequence , the sequence information available for this genome in NCBI GenBank http://www.ncbi.nlm.nih.gov/genbank also revealed about 70 VvTPS. A comparison with other plant genomes in which TPS genes have been annotated showed that the grapevine VvTPS gene family is the largest identified to date. The Arabidopsis thaliana genome contains 32 complete AtTPS genes and eight AtTPS pseudogenes , while the rice and poplar genomes are predicted to encode 40 to 50 TPS-like genes according to  and unpublished results (S. Aubourg).
The 69 candidate VvTPS sequences identified as intact or potentially intact represent five of the seven plant TPS gene subfamilies TPS-a through TPS-g previously described [16,33,37] (Figure (Figure1;1; Additional File 1). The TPS-f subfamily of Clarkia brewerii linalool synthase-like genes and the gymnosperm-specific TPS-d subfamily , were the only subfamilies missing full-length VvTPS members. Although the previous 8-fold grapevine genome assembly  contained one VvTPS-f subfamily member, in the manually curated assembly and annotation of the 12-fold genome sequence, this gene was now fragmented into two partial TPS (Additional File 1).
The TPS-a subfamily is substantially extended in grapevine with 30 VvTPS existing on just two chromosomes, chromosomes 18 and 19, compared to 22 AtTPS of the TPS-a subfamily in A. thaliana . This subfamily typically contains sesquiterpene synthases and possibly diterpene synthases of secondary metabolism. A total of 19 VvTPS were found in the TPS-b subfamily of angiosperm monoterpene synthases and these were located on at least three chromosomes, chromosomes 8, 12 and 13. The TPS-g subfamily, which contains synthases for acyclic monoterpenes of floral scent  is greatly extended in grapevine with 17 VvTPS annotated compared to Arabidopsis with just one AtTPS gene in this subfamily. The chromosomal location of most of the VvTPS of the TPS-g subfamily is unknown. We found two VvTPS of the TPS-c subfamily and one VvTPS of the TPS-e subfamily. These two subfamilies contain TPS genes of primary plant hormone metabolism that are not typically represented with multiple gene copies in plant genomes [16,33,38].
The topological organization of the VvTPS family in the grapevine genome is characterized by massively tandemly repeated genes. Of the complete set of 152 VvTPS loci identified in this study, 129 (85%) are organized in 13 distinct clusters covering from 2 to 45 VvTPS genes or pseudogenes (Additional File 1). The largest VvTPS cluster, localized on chromosome 18 and spanning 690 kb, contains 20 complete VvTPS genes (all are members of the TPS-a subfamily), 25 VvTPS pseudogenes and numerous traces of Copia-like retrotransposons (Figure (Figure2).2). Although many VvTPS cluster together, of the 152 VvTPS loci, only 2 VvTPS genes also localize in the vicinity of other putative terpenoid pathway genes with the same gene orientation: one (VvTPS42) co-localizes with a prenyltranferase gene and the other (the pseudogene GSVIVT01014893001) with a cytochrome P450 gene. Dynamically expanding or contracting clusters of closely related genes can evolve as the result of unequal cross-over, which enriches genetic variability but limits the divergence through an opposing mechanism of gene conversion as has been shown for plant resistance genes [39,40]. These processes can be intensified by the presence of pseudogenes which contribute to the frequency of crossing-over and increase in gene diversity . As previously shown for the Drosophila melanogaster genome, a high density of repeat elements can also impact the recombination dynamic within gene clusters . The genome architecture of the VvTPS gene family (i.e., the number, size and nature of VvTPS clusters in the grapevine genome) suggests a large potential for diversification and variation of terpenoid metabolism in this species, and may thus account for variability of terpenoid profiles among grapevine varieties and cultivars. The identification of VvTPS gene clusters allows for future work in which resequencing of these regions in different varieties and testing for associations of gene cluster and terpenoid aroma traits can be undertaken.
In agreement with highly conserved intron-exon structure of plant TPS genes [16,43,44], all but one of the 66 VvTPS genes of the subfamilies TPS-a, TPS-b and TPS-g contain seven coding exons (Figure (Figure3).3). The only exception is VvTPS17 (TPS-a) in which the 3'-most exon was disrupted by a large and probably recent intron insertion. The three genes of the subfamilies TPS-c and TPS-e are characterized by longer sequences (15 and 13 exons respectively) as a consequence of the presence of an additional exon encoding an ancestral 200 amino acid N-terminal domain of unknown function [16,33,38].
The grapevine VvTPS protein family is characterized by two large domains defined in the PFAM resource : PF01397 corresponds to the N-terminal region and PF03936 corresponds to the C-terminal metal cofactor binding domain . Just upstream of the PF01397 N-terminal domain, in the region encoded by the first exon, all VvTPS that putatively function as monoterpene synthases, also contain the RR(x)8W motif. This motif may play a role in the initiation of the isomerization-cyclization reaction  or act to stabilize the protein through electrostatic interactions ; however, TPS in subfamily TPS-g, as well as two VvTPS predicted in TPS-a, and those in TPS-c and TPS-e do not consistently contain this motif or they have a highly modified version of it (Figure (Figure33 and and4A).4A). Several of the TPS-g members are also truncated with the starting M at position five of this motif. This may effectively open up the three dimensional structure of these TPS or it may affect subcellular compartmentation of these proteins. Mono- and diterpene synthases typically contain an N-terminal plastidial targeting peptide upstream of the conserved or modified RR(x)8W , and such targeting peptides have been predicted in silico for 21 VvTPS (Figure (Figure33).
The C-terminal domain contains two highly conserved aspartate-rich motifs. The first of these, the DDxxD motif (Figure (Figure3),3), is involved in the coordination of divalent ion(s), water molecules and the stabilization of the active site [46,49,50]. Only four predicted VvTPS (VvTPS48, VvTPS66 and the two TPS-c proteins VvTPS67 and VvTPS68) lack the exact DDxxD motif characteristic of class I TPS which catalyze reactions initiated by cleavage of the diphosphate group of the prenyl diphosphate substrate. The TPS-c proteins are not expected to have this domain as they do not cleave the prenyl diphosphate unit; however, they do contain the DXDD sequence critical to the protonation initiated reaction mechanism of class II TPS .
A second important motif in the C-terminal domain is the NSE/DTE motif [52,53]. The reported consensus sequence of this motif is (L,V)(V,L,A)(N,D)D(L,I,V)x(S,T)xxxE and a modified version, (L,F)(M,I,S,C,W)(N,D)D(L,M,I)x(S,T,D)xxxE, is found in almost all VvTPS of the subfamilies TPS-a, -b, and -g (Figure (Figure4B).4B). Three predicted VvTPS are lacking the terminal E. Members of the TPS-g subfamily have an altered and highly conserved sequence, LWDDLx(S,T)xxxE.
Since specific functions of TPS genes cannot be accurately predicted from sequence analysis alone, it was important to clone and functionally characterize VvTPS full length (FL)cDNAs. We used the previously published 8-fold  and the new 12-fold genome sequence (GenBank, NCBI project ID 18785) assembly of the Pinot noir inbred line for primer design to clone VvTPS FLcDNAs from three grapevine varieties, Pinot noir (PN), Cabernet Sauvignon (CS), and Gewürztraminer (Gw). FLcDNAs were expressed in E. coli using one of several cloning vectors (see Materials and Methods), and recombinant VvTPS proteins were functionally characterized via purified protein or in vivo recombinant E. coli assays with each of the following potential substrates, geranyl diphosphate (C10, GPP), (E,E)-farnesyl diphosphate (C15, FPP), and (E,E,E)-geranylgeranyl diphosphate (C20, GGPP). Although a couple of recent studies identified two tomato TPS which utilize neryl diphosphate or (Z,Z)-farnesyl diphosphate as substrates [54,55], these TPS are members of the TPS-e subfamily and none of the VvTPS that we characterized belonged to this subfamily. In addition, a screen of the grapevine genome sequence did not reveal the presence of Z-isoprenyl diphosphate synthases (unpublished results, M. Chavez, S. Aubourg, and J. Bohlmann), therefore, we did not include these alternative substrates when assaying for VvTPS activity. Products were analyzed by GCMS and the majority of VvTPS analyzed produced multiple products (Table (Table1,1, Table Table2,2, Table Table3,3, and Table Table4),4), which is a common feature of plant TPS [56,57]. Since many of the VvTPS FLcDNAs arose from cloning efforts in all three V. vinifera varieties PN, CS, and Gw, a number of VvTPS were uncovered as cultivar-specific variants, each differing by only a few amino acids. To ensure that these were not unique genes capable of producing a distinct product profile, each variant was functionally characterized. However, only one representative cDNA clone will be described for its function following below, while the additional clones from the other cultivars are listed in Table Table1,1, Table Table2,2, Table Table3,3, and Table Table44.
A subset of VvTPS FLcDNAs of the TPS-a subfamily (Table (Table1)1) were chemically synthesized and characterized using an E. coli strain engineered to produce the FPP substrate from mevalonate. Based on previous work , an operon encoding the mevalonate lower pathway of Streptoccoccus pneumoniae was subcloned into a bacterial expression vector together with the Saccharomyces cerevisiae FPP synthase. VvTPS FLcDNAs were additionally expressed into this engineered strain and product formation was measured by GCMS in the culture extract as has been done for the characterization of other TPS and cytochrome P450 s .
We assigned gene identifiers that include references to both function and the cultivar (PN, CS, or Gw) from which the gene was isolated (see Functional Gene ID in Table Table1,1, Table Table2,2, Table Table3,3, and Table Table4).4). These functional gene identifiers will be used throughout the following sections to describe individual genes. Table Table1,1, Table Table2,2, Table Table3,3, and Table Table44 provide additional detailed information for each FLcDNA regarding clone ID, tissue origin (see table legend), product profiles with relative quantitative information, as well as identification of the closest annotated VvTPS gene model reported in this paper. In some instances multiple cDNAs share the same functional gene identifier, but are represented as distinct genes because they occupy unique locations within the VvTPS phylogeny. These functional gene IDs are designated with numbers in both the tables and within the phylogenetic trees.
The majority of VvTPS genes belong to the TPS-a subfamily for which we functionally characterized 13 unique FLcDNAs (Table (Table1).1). All of the VvTPS-a members were characterized as sesquiterpene synthases, and all but one formed multiple products with FPP as substrate. In several cases, the product profiles included both terpenoid hydrocarbons and alcohols. As a group, the VvTPS of the TPS-a subfamily produce a diverse array of sesquiterpene products.
All five individual VvGwECar and VvPNECar FLcDNA clones produced predominantly (E)-β-caryophyllene. Four of these clones also produced α-humulene and a small amount of germacrene D, while one clone (CAN82172) produced only (E)-β-caryophyllene (94%) and α-humulene (6%). The two VvPNECar enzymes characterized in metabolically engineered E. coli (clones CAO16256 and CAN82172) showed similar product profiles to VvGwECar clones characterized by in vitro enzyme assays. One of the VvPNECar enzyme (CAO16256) also produced a low amount ( < 1%) of an unknown sesquiterpene alcohol in addition to (E)-β-caryophyllene, α-humulene and germacrene D. The TPS VvGwGerA (Gw38F3) produced primarily germacrene A (52%) and α-selinene (24%) and a small amount of selin-11-en-4-α-ol (12%). The product profile of VvGwaBer (Gw56B1) consisted of (E)-α-bergamotene (56%), zingiberenol (17%) and nerolidol (14%) as well as two minor compounds. Germacrene D was the primary product of VvGwGerD (Gw64B1). VvCSAFar (CS102B7) was the only single-product member of the TPS-a subfamily identified here, producing 100% (E,E)-α-farnesene. Two products, γ-cadinene (83%) and an unidentified sesquiterpene (17%), were the only detected products of VvGwgCad (Gw330M5). VvPNbCur (PN62M1) produced β-curcumene (22%) (E)-γ-bisabolene (18%), iso-italicene) (16%), (-)-α-bisabolol (14%), and at least 9 additional products. The VvPNSesq (CAO16252) generated a sesquiterpene olefin as major product that was not identified unambiguously by GCMS. The product was, therefore, produced in larger quantity, purified by preparative GC and identified by NMR spectroscopy as sesquithujene ((1 S,5S)-2-methyl-5-((R)-6-methylhept-5-en-2-yl)bicyclo[3.1.0]hex-2-ene). Additional reaction products of this TPS included α-bergamotene, sesquisabinene, β- and γ-bisabolene, β-bisabolol and a trace amount of an unidentified sequiterpene alcohol. VvPNaZin (CAO16257) is a zingiberene synthase that also produced β-sesquiphellandrene and β-bisabolene. The two major products generated by VvPNSeInt (CAO39293) were found to be selina 4,11-diene and intermedeol. At least, five other sesquiterpenes including α-guaiene, selina 5,11-diene, γ-selinene, and 7-epi-α-selinene, were identified as reaction products. VvPNCuCAD (CAN76781) encodes a multi-product sesquiterpene synthase capable of producing cubebol, δ-cadinene, α-copaene, α-cubebene and an unknown sesquiterpene alcohol as dominant reaction products; additional minor products included α-gurjunene, γ-cadinene, β-cubebene and an unknown sesquiterpene. The VvPNaHum (CAN64791) produced α-humulene (56%), (E)- β-caryophyllene (6.5%) and a sesquiterpene alcohol (37.5%) that we initially failed to identify by GCMS; the latter compound was then produced in milligram quantities, purified by liquid chromatography and identified by 1H- and 13C-NMR spectroscopy as hyemalol (3,7,10,10-tetramethylcycloundeca-3,7-dien-1-ol) a humulane-type sesquiterpenoid recently discovered in Zanthoxylum hyemale . VvPNEb/2epiCar (CAO39418), was identified as a third type of caryophyllene synthase, which in contrast to VvPNECar and VvGwECar also catalyzed the formation of 2-epi-(E)-β-caryophyllene as a substantial (25%) product.
Collectively, these characterized VvTPS-a enzymes produce some of the major sesquiterpenes identified from grapevine. Furthermore, the prevalence of VvTPS producing (E)-β-caryophyllene, α-humulene and germacrene D may in part explain the reported prominence of these compounds in grapevine berries and vegetative tissues [12-15].
We functionally characterized seven unique VvTPS (nine different FLcDNA clones) from the TPS-b subfamily (Table (Table2).2). All of the characterized VvTPS genes of this group produce monoterpenes, and most are multi-product enzymes. The products of VvGwaPhe included (+)-α-phellandrene (40%), myrcene (15%), terpinolene (15%), and seven other minor products. VvPNaPIN produced (+)-α-pinene (47%), (+)-limonene (25%), (+)-camphene (9%), and eight other minor products. Five of the nine VvTPS of the TPS-b subfamily (VvGwbOci, VvCSbOci, VvCSbOciM, VvGwbOciF, VvCSbOciF) produced (E)-β-ocimene as a major product with individual variations of additional products. VvGwbOci and VvCSbOci produced additional minor amounts of (Z)-β-ocimene; VvCSbOciM produced additional major amounts of myrcene (42%) along with minor amounts of β-pinene and limonene; VvGwbOciF and VvCSbOciF also converted FPP into (E,E)-α-farnesene. Lastly, VvPNRLin produced a single oxygenated product, (3R)-linalool. Together the VvTPS-b subfamily members account for many of the acyclic and cyclic monoterpene hydrocarbons and a few of the monoterpene alcohols found in Vitis vinifera.
The TPS-g subfamily is greatly expanded in V. vinifera (Figure (Figure2,2, Addiitonal File 1). Functional characterization of ten different FLcDNA clones of this subfamily identified three unique gene functions (Table (Table3).3). All VvTPS of this group produce exclusively acyclic terpene alcohols, but the three types differ by their range of substrates. The first type of VvTPS gene function within the TPS-g subfamily is represented by three genes, VvPNLinNer1, VvPNLinNer2, and VvCSLinNer. These enzymes accept two substrates, C10 GPP and C15 FPP, and produce (3S)-linalool and (E)-nerolidol, respectively (Table (Table3).3). The second group is represented by four VvPNLNGl enzymes which also accept the additional C20 substrate GGPP to produce (E,E)-geranyl linalool. The third unique function in this subfamily is represented by three genes, VvGwGer, VvCSGer and VvPNGer, which had activity only with GPP to produce geraniol. While seven of the VvTPS of the TPS-g subfamily accept more than one substrate in vitro and contribute potentially to the formation of terpene alcohols of different chain lengths, it is not known whether these enzymes indeed encounter more than one type of substrate in vivo.
Although the analysis of the 12-fold genome sequence coverage did not identify any intact VvTPS genes of the TPS-f subfamily, a unique VvTPS function of the TPS-f subfamily was characterized with the two FLcDNAs VvCSENerGl and VvPNENerGl (Table (Table4).4). These enzymes accepted either FPP or GGPP to produce (E)-nerolidol or (E,E)-geranyl linalool, respectively. Unlike the VvTPS of the TPS-g these enzymes had no activity with GPP. VvCSENerGl and VvPNENerGl are only 62% identical and 76% similar on an amino acid level.
The phylogenetic analyses presented here include V. vinifera TPS from the 12-fold sequence assembly of the nearly homozygous Pinot noir genotype  and the functionally characterized VvTPS described here. The analyses also included full-length TPS sequences that contained the known TPS motifs predicted from the genome assembly of the heterozygous Pinot noir genotype  for a more complete annotation of the VvTPS family. In this way, we have integrated the predictions of VvTPS gene models from the two grapevine genome sequences [1,36] in a compatible fashion and we are proposing a unified VvTPS classification and nomenclature.
Within the TPS-a subfamily of sesquiterpene synthases the functionally characterized VvTPS are close to most of the VvTPS predicted in the 12-fold genome sequence assembly (Figure (Figure5).5). This topology suggests that the diversity of functions for grapevine sesquiterpene synthases is well represented with the functionally characterized VvTPS described in this work. Relative to TPS-a enzymes of other plant species, the VvTPS exhibit a large paralogous cluster with VvTPS-a members more closely related to one another than they are to TPS from other species, regardless of function. Paralogous TPS gene clusters were found previously for other species examined in depth such as A. thaliana  and Norway spruce  and indicate post-speciation gene duplication events. The large number of VvTPS-a suggests that this subfamily plays an important role in grapevine biology.
VvTPS of the TPS-b subfamily fall into two clades, VvTPS-b clade I and VvTPS-b clade II, bifurcated by representative TPS from other plants (Figure (Figure6).6). The majority of the VvTPS of clade I make cyclic products while those of clade II produce only acyclic terpenoids. It is possible that clade-specific conserved sequence features determine whether a TPS is able to produce cyclic or acyclic products ; thus, the two clades may represent an evolutionary pattern of sub-functionalization from cyclic-product TPS in clade I to those TPS producing acyclic products in clade II. In contrast to other TPS subfamilies, the VvTPS clades of the TPS-b subfamily include members that have functional equivalents in distantly related species. For example, Lotus japonica (E)-β-ocimene synthase clusters closely with grapevine ocimene synthases. Malus x domestica (E,E)-α-farnesene synthases also clusters closest to VvTPS of the same function. This pattern suggests that these functions arose prior to speciation events.
The TPS-g subfamily of plant TPS was defined by previous work on TPS of floral scent biosynthesis in snapdragon (Anthirrrhinum majus) . Phylogenetic analyses that include the large number of VvTPS gene (Figure (Figure1)1) conclusively resolved a bifurcation of the TPS-b and TPS-g subfamilies at a juncture that was previously ambiguous and had misclassified some TPS-g genes as TPS-b members. Specifically, the newly characterized grapevine geraniol synthase VvPNGer which matches gene model VvTPS52 (Figure (Figure1)1) as well as the geraniol and linalool synthases from basil (Ocimum basilicum) (Figure (Figure7),7), clustered closely with the TPS-g subfamily. The phylogenetic proximity between the basil and grapevine geraniol synthases indicates that these TPS functions already existed in a common ancestor. In contrast, the remaining VvTPS of the TPS-g subfamily, which are all linalool/nerolidol synthases, cluster closest to other VvTPS. As mentioned above, the entire predicted VvTPS-g subfamily has a conserved NSF/DTE motif (Figure (Figure4).4). This same motif is present in the cloned VvTPS cDNAs as well as other members of the TPS-g subfamily from different species. Prominent in this motif of the VvTPS-g members is a W in the second position; this residue may affect the magnesium binding and/or substrate orientation. Also noteworthy is the highly modified or absent RRX8W motif from this group of TPS (Figure (Figure4)4) and which may imply that these acyclic products are formed via the geranyl cation rather than the linalyl cation [37,53].
The TPS-e and TPS-c subfamilies in V. vinifera contain one and two members, respectively (Figure (Figure8).8). Although these were not functionally characterized in this paper, they are almost certainly involved as diterpene synthases in ent-kaurene biosynthesis . Surprisingly, the 12-fold sequence coverage of the grapevine genome did not reveal any members of the TPS-f subfamily; however, our FLcDNA cloning identified two members of this subfamily and both were characterized as nerolidol/geranyl linalool synthases (Figure (Figure8).8). These are related to the Clarkia brewerii linalool synthases  and the recently characterized A. thaliana geranyl linalool synthase , each of which produces acyclic terpene alcohols as a functional signature of this subfamily.
The present study provides the first comprehensive annotation of the very large VvTPS gene family with regard to chromosomal localization, enzyme functions, and phylogeny relative to the overall plant TPS gene family. The VvTPS gene family is one of the largest gene families of specialized (i.e., secondary) metabolism in grapevine where TPS enzymes contribute to berry and wine flavour, floral scent and potentially a diversity of other biological functions such as defense and resistance. The emerging profile of the VvTPS family described here illustrates how this large gene family has expanded across the genome through gene duplication events and functional diversification. Notably, the large number of functionally diverse sesquiterpene synthases identified in our biochemical characterization of the VvTPS-a genes suggests that these enzymes and their products may contribute substantially to grapevine biology and wine quality. The recent reports of sesquiterpenes in Shiraz grapes and wine [10,11] or the identification of sesquiterpene volatiles in anthers and pollen of grapevine flowers  are early insights to the emerging roles for sesquiterpene metabolism in V. vinifera.
Phylogenetic analyses of the VvTPS show a result similar to many plant species studied thus far in that most of the VvTPS form clusters of paralogous genes within the plant TPS family. This finding indicates a dominant process of post-speciation gene duplications, although there are also examples of conserved TPS functions of a more ancient order. Furthermore, our analyses substantiate separation of the TPS-b and the TPS-g subfamilies. The separation based on sequence relatedness is matched by separation of gene functions, since all known members of the TPS-g subfamily produce acyclic products.
Of the monoterpene synthases presented here, those that produce (3S)-linalool (VvTPS-g), geraniol (VvTPS-g), and the previously identified α-terpineol synthase (VvTPS-b) will be of much interest to viticulturalists and wine makers as these are some of the most prevalent compounds responsible for the floral characteristics of aromatic varieties. Furthermore, compounds such as geraniol and linalool can be further modified in grape musts and wine to produce citronellol, rose oxide, and wine lactone [63,64]. Linalool and α-terpineol have also been found to contribute to the character of non-aromatic red grapevine varieties . It is conceivable that different viticultural regimes may modify the expression of these TPS in grape berries and can thereby impact the quality of the resultant wines.
While many of the terpenoid products of the VvTPS enzymes characterized here have been described in the viticulture and oenology literature [8,66], still several have yet to be associated with traits in grapes or wine. Taken together, the present VvTPS genomic annotations and the VvTPS functional characterizations provide a reference framework for future studies, including transcript and protein expression profiling, as well as terpenoid molecular marker development through, for example association mapping.
The predicted proteome of the 12-fold coverage grapevine genome sequence assembly (GenBank, NCBI project ID 18785; Genoscope website: http://www.genoscope.cns.fr/externe/GenomeBrowser/Vitis/) was screened with two HMM profiles of the PFAM motifs  PF01397 (N-terminal TPS domain) and PF03936 (TPS, metal binding domain). In addition, the 12-fold genome sequence assembly was screened (TBLASTN) with known TPS sequences from Swiss-Prot in order to be not dependent of the automatic annotation. The 152 loci exhibiting significant similarities with known TPS (all BLAST hits with an e-value lower than 1.e-4 were individually evaluated) were manually annotated to correct erroneous automatic annotation and to discriminate between complete, partial and pseudo-TPS. Genomic regions with similarities spanning on less than 50 amino acids with TPS have not been considered. The manual annotation is based on the results of the EuGène predictor-combiner software  that was specifically trained for Vitis vinifera, sequence alignments with previously characterized TPS proteins and related PFAM motifs, spliced alignments  of cognate EST and cDNA sequences and knowledge of TPS gene structure and protein sequences. Data and other related information were imported and merged in the ARTEMIS tool  to evaluate each resource and produce the final annotation. The EuGène predictions, the manual structural annotation of the 152 loci and the corresponding sequences are available in the FLAGdb++ database http://urgv.evry.inra.fr/FLAGdb. Protein sequences deduced from the 69 full VvTPS genes were analyzed with ChloroP for prediction of N-terminal plastidial targeting peptides 
Amino acid alignments were made using Dialign (dialign.gobics.de/anchor/submission.php) with a threshold value of 10. Manual adjustments such as aligning conserved motifs and manual trimming were performed using GeneDoc http://www.nrbsc.org/gfx/genedoc. For all analyses, sequence information upstream of the partially conserved RR(X)8W motif was trimmed. Maximum likelihood analyses were completed using Phyml  available at http://www.atgc-montpellier.fr/phyml/. For each analysis, the LG amino acid substitution model and four substitution rate categories were used, the proportion of invariable sites and the gamma distribution parameter were estimated, and the branch lengths and tree topology were optimized from the data set. The estimated values for the proportion of invariable sites and the gamma shape parameter were then used when performing 100 bootstrap replicas. Phylogenetic trees were visualized using TreeView http://taxonomy.zoology.gla.ac.uk/rod/treeview.html.
RNA was isolated from Gewürztraminer, Pinot noir, and Cabernet Sauvignon grapevine shoot cuttings grown in the greenhouse as previously described . RNA was isolated from stem (S), leaf (L), berry (B), root (R) and flower (F) tissues (see FLcDNA ID in Table Table1,1, Table Table2,2, Table Table3,3, and Table Table4)4) as detailed in Reid et al. . To up-regulate expression of TPS genes, a subset of grapevine cuttings were treated with methyl jasmonate (0.01% v/v in water and 0.1% Tween) two days prior to RNA isolation. The Superscript Vilo cDNA synthesis kit (http://www.invitrogen.com was used according to the manufacturer's instructions. Primers for VvTPS cDNA cloning were designed based on TPS sequences obtained through iterative BLAST searches in NCBI GenBank using members of each of the TPS subfamilies. Additional information for the design of PCR primers came from the predicted VvTPS gene models identified in the 12-fold genome sequence assembly. To increase the likelihood of a successful PCR amplification of VvTPS cDNAs, cDNA templates from various tissues from the same cultivar were often combined. These clones are designated "M" for mixed template while those TPS cloned from individual tissues are labeled with the single letter abbreviations described above (Table (Table1,1, Table Table2,2, Table Table3,3, and Table Table4).4). PCR reactions were done using touchdown PCR with proofreading polymerases, as per the product manufacturer's instructions. PCR products of the expected sizes were cloned into the pJet1.2 cloning vector http://www.fermentas.com and transformed into E. coli α-select cells http://www.bioline.com. Plasmids containing a correctly sized insert were sequenced followed by insert amplification and ligation into the pET28b expression vector (Novagen, http://www.emdchemicals.com) using sticky end PCR . When cloning difficulties were encountered, as was the case with several sequences, pASK-IBA3plus (IBA, http://www.iba-go.com) vector was used .
For TPS protein expression, Cip41 Rare E. coli cells  containing recombinant VvTPS plasmids were grown until 0.8 OD, induced with 0.5 mM IPTG and then grown for an additional 16 h at 16 °C. Recombinant VvTPS were partially purified using His SpinTrap columns (GE Biosciences, http://www.apbiotech.com). Protein expression was verified using silver stained SDS-PAGE gels and western blot analysis using Murine anti-polyHistidine Monoclonal antibody (1/4000 dilution, Sigma-Aldrich, http://www.sigmaaldrich.com) and 5-bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium (CalBiochem, http://www.emdchemicals.com). For Strep-tag II clones, the pASK-IBA3plus vector combined with Cip41 Rare cells were used for expression and protein was isolated as per the manufacturer's instructions using a Strep-Tactin affinity purification column (IBA).
For functional enzyme characterization, approximately 100 μg total protein of semi-purified recombinant VvTPS were added to a single vial assay in 50 μL total volume as described [32,75,76]. Each recombinant VvTPS was incubated with GPP (109 μM), (E,E)-FPP (92 μM) or (E,E,E)-GGPP (20 μM) for 2 h at 30 °C. Buffers used for sesquiterpene and diterpene synthase assays contained 50 mM HEPES pH 7.0, 10 mM MgCl2, 20 μM MnCl2, 10% glycerol (v/v), and 1 mM DTT. For monoterpene synthase assays, the buffer consisted of 50 mM HEPES pH 7.0, 10 mM MgCl2, 20 μM MnCl2 100 mM KCl, 10% glycerol (v/v), and 1 mM DTT.
Gas chromatography coupled with mass spectrometry (GCMS) analysis was employed to determine the product profiles of each TPS as previously described [32,56]. Columns used to separate product mixtures included HP-5MS (Agilent), DB-Wax (Agilent), SolGelWax (SGE), and Cyclodex-B (Aglient) for chiral analyses. For sesquiterpene analysis, a cool-on column inlet (starting temperature of 35 °C and track oven program after injection) was used to prevent thermo-rearrangements of terpenes in the injector. Identities of individual terpenes were made using a combination of authentic standards and/or retention indices along with MS library matches (including WileyNist 2005, , and/or MassFinder4 [massfinder.com/wiki/MassFinder_4]).
A subset of VvTPS encoding sesquiterpene synthases were codon-optimized for expression in E. coli, using gene synthesis by DNA2.0 https://www.dna20.com/ and subcloned into the pET-3a or pET-Duet1 expression plasmids (Novagen, http://www.emdchemicals.com). Enzymatic activity was assessed in E. coli cells co-expressing the TPS together with the enzymes of a five-step biosynthetic pathway converting mevalonic acid to FPP. The FPP synthase gene was amplified from S. cerevisiae genomic DNA and ligated into the first multiple cloning site (MCS) of the pACYCDuet-1 expression plasmid (Novagen, http://www.emdchemicals.com) providing the plasmid FPPs-pACYCDuet. An operon encoding the genes for a mevalonate kinase (MvaK1), a phosphomevalonate kinase (MvaK2), a mevalonate diphosphate decarboxylase (MvaD) and an isopentenyl diphospahte isomerase (idi) was amplified from genomic DNA of Streptococcus pneumoniae (ATCC BAA-334) and ligated into the second MCS of the FPPs-pACYCDuet plasmid providing the plasmid pACYCDuet-4506. BL21 Star™(DE3) E. coli cells http://www.invitrogen.com were co-transformed with the plasmids, pACYCDuet-4506 and either of the pET series plasmids harboring candidate VvTPS coding sequences. Single colonies were used to inoculate 5 mL of LB medium supplemented with carbenicillin (50 mg/mL) and chloramphenicol (34 mg/mL). Cultures were incubated overnight at 37°C. The next day 250 mL to 1 L of Terrific Broth medium supplemented with the appropriate antibiotics were inoculated with 1/100 volume of the overnight culture. After 6 h incubation at 37°C, cultures were cooled down to 28°C and then 1 mM IPTG, 2 mg/mL mevalonate prepared by dissolving mevalonolactone http://www.sigmaaldrich.com in 0.5N NaOH at a concentration of 1 g/mL and incubating the solution for 30 min at 37°C) and 0.1 volume of decane were added to the cultures. After 48 h incubation, the decane fraction was directly analysed by GCMS on a Hewlett Packard 6890 series GC system equipped with a DB1 column 30 m × 0.25 mm × 0.25 mm film thickness http://www.agilent.com and coupled with a 5975 series mass spectrometer. The carrier gas was helium at a constant flow of 1 ml.min-1. Injection was in splitless mode with the injector temperature set at 120°C and the oven temperature was programmed from 60°C to 265°C at 5°C.min-1. Identification of VvTPS products was based on retention time index, mass spectra of authentic standards, and on published  or Firmenich MS database. Co-injections were also used for some of the compounds (caryophyllene, humulene, cubebol, cubebene, intermedeol). For compounds that could not be identified by GCMS, the products were purified and their structures determined by NMR spectroscopy. Sesquithujene was isolated using manual, preparative-GC. The crude sample (bi-phasic bacteria culture) was first distilled using a Fisher column to remove the decane (temperature: 95 °C; pressure: 25 mbar). The compounds were then separated using a GC equipped with a.1.83 m × 2.1 mm i.d., 10% OV -1 packed column at a flow rate of 10 mL/min. The oven temperature was programmed from 160 °C (held 10 minutes) to 230 °C at 10 °C/min. Helium was used as the carrier gas. Hyemalol was purified by silica gel flash column chromatography (Silicagel 60, 12*150 mm, 40-63 μM, Merck) using a 98:2 mixture of toluene and diethyl ether as solvent system. NMR data were acquired at 298 K using a Bruker Avance 500 MHz spectrometer. The structure was established by 1 D 1H- and 13C- NMR, as well as 2 D HSQC, COSY and HMBC experiments.
VvTPS: Vitis vinifera terpene synthase; AtTPS: Arabidopsis thaliana terpene synthase; FLcDNA: full length cDNA; PN: Pinot noir; CS: Cabernet Sauvignon; Gw: Gewürztraminer; FPP: farnesyl diphosphate; GPP: geranyl diphosphate; GGPP: geranylgeranyl diphosphate.
DMM, OT, MBS, LD, and SA, performed experiments and analyzed data. DMM, JB, LD, MS and SA conceived of the study, interpreted results and wrote the paper. OT, LD, MS and STL reviewed the paper prior to submission and provided valuable comments on the interpretation and presentation of results. JB and STL secured funding. All authors read and approved the final manuscript.
The Excel file contains all the information relative to the 152 TPS loci detected and curated in the 12-fold PN40024 grapevine genome. The content of each column is: - Name: VvTPS nomenclature for the 69 complete terpene synthases. - Gene ID (12x): The official ID of the gene automatically annotated by IGGP (International Grape Genome Program) and used by GenBank/EMBL. The genes called 'newX' correspond to TPS loci completely missed by the automatic annotation pipeline. The curated gene structures and sequences are available in the FLAGdb++ database http://urgv.evry.inra.fr/FLAGdb. - merged ID: If the re-annotated TPS genes fit or overlap with several IGGP consecutive genes (erroneous splitting of the automatic annotation), their IDs are mentioned here. - Prot size: The size (in amino acids) of the re-annotated TPS proteins. For not complete or coherent CDS (partial or pseudo-TPS), the size fits with the longest rebuilt protein sequence (in italic). - Chr: The chromosome number. '10R' means that the gene is on the chromosome 10 but on a not mapped scaffold. 'R' means that the gene is inside an unmapped scaffold. - Exons: Number of annotated exons in the CDS (after curation). - Subfamily: Classification of TPS (TPS-a to TPS-g) according to the phylogeny and functional studies. The table is colored according to this column. - DDxxD: 'yes' means that the exact motif is present at the expected position (end of the exon 4). '?' means that the corresponding part of the gene is absent (partial gene or pseudogene). - RRx(8)W: 'yes' means that the exact motif is present at the expected position (end of the exon 1). '?' means that the corresponding part of the gene is absent (partial gene or pseudogene). - NSE/DTE: sequence of the NSE/DTE motif present in the C-terminal region of the proteins from TPS-a, -b and -g subfamilies. - Start: first position of the curated CDS in the 12-fold pseudo-chromosomes. - Stop: last position of the curated CDS in the 12-fold pseudo-chromosomes. - Strand: 'm' means minus strand and 'p' means plus strand relatively to the pseudo-chromosomes. - Manual re-annotation result: information about the evaluation and curation process. Protein IDs are listed for the VvTPS genes where automatic annotation predicted a correct structure. - Type: 'full' means that the TPS gene (CDS) is complete, without sequence problem. 'full ?' means that CDS is complete excepted one punctual sequence problem. A sequencing error is therefore possible and the gene could be functional. 'partial' means that the gene is disrupted by an un-sequenced region (gap of N) and additional sequencing is necessary to have a full CDS. 'pseudo' means that the gene structure is disturbed by stop(s) in frame, frameshift(s) and/or deletion(s). As it is, the gene cannot be functional and is qualified as pseudogene. - EST: The number of available cognate transcript sequences. (+) means that the CDS is fully covered by the EST contig. - Note: Additional information about the partial genes and pseudogenes (nature of the problem, gap positions...). - Cluster: TPS with the same letter are organized in tandem in the same physical cluster. - Subcell. loc.: The result of the ChloroP prediction tool. A score greater than 0.5 means that the TPS protein is probably targeted to the plastids. 'x' means that the prediction has not been done because the N-terminal region of the protein is lacking. - Best hit in SwissProt: Biochemical function of the first hit obtained in the SwissProt database with BLASTP.
We are grateful to Eric J. Abbott for bioinformatics and technical work on the cloning of TPS. We acknowledge Vincor International for allowing us to collect grape shoot material from their vineyards in Okanagan Falls, BC, and Osoyoos, BC. DMM was supported by a postdoctoral fellowship from the Natural Sciences and Engineering Research Council of Canada (NSERC). Salary support for JB was provided, in part, by the UBC Distinguished University Scholar program. Funding for this project was provided by the Natural Sciences and Engineering Research Council of Canada (to JB), Genome British Columbia, and Genome Canada (to JB and STL). Fabienne Deguerry, Laurence Pastore and Eggen Xian-Wen Gan (Firmenich SA) are thanked for excellent technical assistance.